ISEfa18
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP041262 | ND | Enterococcus faecium | Enterococcus faecium Enterococcus faecium VVEswe-R plasmid pVVEswe-R1 |
DNA section
IS Length : 2345 bp
Ends
IR Length : 25
IRL : GTAAGCGCCCCATAGAACAGTACCTTACTAACAAATGCACCTCAAGATAT
IRR : GTAAGCGCCCCATAGAACAGTACCTGTACGTCAAATAACCCGACCTATAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAAATCAACGT | TTTTCAAC | TTTTGAAATG | 8 |
DNA sequence
GTAAGCGCCCCATAGAACAGTACCTTACTAACAAATGCACCTCAAGATATACTTGAGCTATCAAGAAGTCTGGAGGTTTTCTATGTATAAATTAAATGAG
ATTGTTCCTGTCCAATTAGGAAACGAAAAGAGTGCACCAGAAAAAGGTAAACCCGCGGCTGCCAAGTTAGCGATGCGCATCAAAAGAGAGAATGGTTCTG
AAGTTTTGATCTATAACGGTATTAACAATTATATTCTTCAAGCTATTTTGAAAGAACTGTTGTCCAATGCTTTATGATTTACTTCAAACCAAACATATTT
ATATTGTGTGCGGAAAAACAGATTTGCGAAAAGGAATTGATGGTCTTGCCAGTCTTATTCAACAAGAGTATCAACTCGAGCTTTACGAAGATGCCGTTTT
TTTATTTTGCGGGAACCGTCAAGACCGTTTCAAACTTCTCTATTGGGACGGTGATGGTTTTCTTCTCTGTTATAAGCGGATAGAAAACGGCAAGTTAAAA
TGGCCTCGGACGAAAGACGAAGTTCGAACCTTAACGAACCAACAGGTTCGATGGTTGTTAGAAGGATTAAGTATCGATCAACCTCGAGCTATTTTGCCAG
GAAAAAAAGGAGTTTTTTAAGCTGGGGGGTTAACACAAAAAATGTGTTGAACCCCTATTTCTTGTGCGTTTCTTGTGGTATTCTGTAACAAAATCAGTTC
GATTCAAGGTGGTGAAAAATTTTGGAAACTACAGATACGTTGCTTCAGTTGCTTCAAGAAGCCCATAAAACCAATCAAGCACAACAACAAACCATTCAGA
ATCTTACAACTGAAATCCAACTATTGAATGAAAAGGTCAATTATTTAACCAACAAATTATTTGGACGCTCTAAAGAGACTCTCTTTGAGGAAACGAATGG
GCAATTGAATCTTTTTAGTGATGAAGAAATTTCTGTAAGTGTTCCAGAAGCAGCTGCCACCATTATTCCAGTAAAAGGACATCAACGAGTTGTAGGAACG
AAAGCAGACAAAATCAAACACTTACCTATAACAGAGAAAGAACATCTCCTTCCTTTGGAAGAACAATTTTGTGAACATTGCGGTTCACAGATGAAAGATA
TCGGACGGACAAAAGTACGAGAAGAAATTCGTTTCCATCAGGCTATGTTGGATTGTCTCACCCATTATCAACATACCTATTGTTGTAAAAGCTGCGAAAA
AGAAGGACTTTCTTCTTTTAAAAAAGCGATCGTTCCTAAACCTTTGATTTCTAATAGCTTAGGTTCCAATAGTTTAGTGGCGGAAACGATCCGGATGAAA
TTTGGTCAGAAAGTTCCTGCCTATCGGCAAGAGAACTATTGGAAACAAACACATGGGTTGGATATTTCGAGAGATAATATCACCAATTGGCATATTAAAG
CAGTGCAAAACGCGTTAGACCCTTTAGGAGAACGATTAAGAGTCTATCTTAATCAAGAAGAGATTCTACACGGGGATGAAACAAGTTATCGTGTGATTGA
AAGTGCCAAAACAGATACCTATTACTGGCAGTTTTGTACAGGAAAAGACAGTCAGCATCCCATCGTTTATTATCACCACGACGAAAGTCGTGCTGGAGAT
GTTCCGAAAACTTTTCTCAAGGAGTTTACAGGGTATTTACACTGTGACGGCTATAGCGGGTATAATGCCGTGGAAAGTGTACGACTTGTCTATTGTTTCG
CGCATGTTCGGCGGAAATTTTTCGAAGCAATTCCAAAAGGAAAGAAAAACACGGATATTCCCGCAGCTCAAGCAGTCAAACAATTGGATAAATGGTTTGT
CCTAGAAAAGAAATGGAAAGACTTTTCTCCTGAGAAGCGATTAAGCTGTCGGCAACAAGAACTGCGTCCATTATTTATTGCTTTTTATGAGTGGATGGCA
ACGATAGATCCTGTTGCAAAATCCAAGTTGGATGCAGCCGTTCAATATGCGTGTAAACTACGAAGTGGTTTTGAACCTATTTTTGAAGATGGTCGTTTAG
AACTCACAAATAATCGTGCGGAGCGAAATATCAAAGAGCTGGTGATAGGACGGAAAAATTGGTTGCATTCCACAAGTCTTGAAGGAGCACGAACTTCAGG
AATCATTCTGAGTGTATATAAAACAGCAGAACTTAACGGATTGAATCCTGTAAAGTATCTAGAGTTTCTATTTGATAAGATACCAAATCTTCCCGTACTT
TCAGCAGAAACTTTAGATCAACTGTTGCCTTGGAACAAGGATGTACAACAACATTTTTCTCGTAACTAAAAACGCCAAACATTCCCGATTCTATTCTATA
GGTCGGGTTATTTGACGTACAGGTACTGTTCTATGGGGCGCTTAC
ATTGTTCCTGTCCAATTAGGAAACGAAAAGAGTGCACCAGAAAAAGGTAAACCCGCGGCTGCCAAGTTAGCGATGCGCATCAAAAGAGAGAATGGTTCTG
AAGTTTTGATCTATAACGGTATTAACAATTATATTCTTCAAGCTATTTTGAAAGAACTGTTGTCCAATGCTTTATGATTTACTTCAAACCAAACATATTT
ATATTGTGTGCGGAAAAACAGATTTGCGAAAAGGAATTGATGGTCTTGCCAGTCTTATTCAACAAGAGTATCAACTCGAGCTTTACGAAGATGCCGTTTT
TTTATTTTGCGGGAACCGTCAAGACCGTTTCAAACTTCTCTATTGGGACGGTGATGGTTTTCTTCTCTGTTATAAGCGGATAGAAAACGGCAAGTTAAAA
TGGCCTCGGACGAAAGACGAAGTTCGAACCTTAACGAACCAACAGGTTCGATGGTTGTTAGAAGGATTAAGTATCGATCAACCTCGAGCTATTTTGCCAG
GAAAAAAAGGAGTTTTTTAAGCTGGGGGGTTAACACAAAAAATGTGTTGAACCCCTATTTCTTGTGCGTTTCTTGTGGTATTCTGTAACAAAATCAGTTC
GATTCAAGGTGGTGAAAAATTTTGGAAACTACAGATACGTTGCTTCAGTTGCTTCAAGAAGCCCATAAAACCAATCAAGCACAACAACAAACCATTCAGA
ATCTTACAACTGAAATCCAACTATTGAATGAAAAGGTCAATTATTTAACCAACAAATTATTTGGACGCTCTAAAGAGACTCTCTTTGAGGAAACGAATGG
GCAATTGAATCTTTTTAGTGATGAAGAAATTTCTGTAAGTGTTCCAGAAGCAGCTGCCACCATTATTCCAGTAAAAGGACATCAACGAGTTGTAGGAACG
AAAGCAGACAAAATCAAACACTTACCTATAACAGAGAAAGAACATCTCCTTCCTTTGGAAGAACAATTTTGTGAACATTGCGGTTCACAGATGAAAGATA
TCGGACGGACAAAAGTACGAGAAGAAATTCGTTTCCATCAGGCTATGTTGGATTGTCTCACCCATTATCAACATACCTATTGTTGTAAAAGCTGCGAAAA
AGAAGGACTTTCTTCTTTTAAAAAAGCGATCGTTCCTAAACCTTTGATTTCTAATAGCTTAGGTTCCAATAGTTTAGTGGCGGAAACGATCCGGATGAAA
TTTGGTCAGAAAGTTCCTGCCTATCGGCAAGAGAACTATTGGAAACAAACACATGGGTTGGATATTTCGAGAGATAATATCACCAATTGGCATATTAAAG
CAGTGCAAAACGCGTTAGACCCTTTAGGAGAACGATTAAGAGTCTATCTTAATCAAGAAGAGATTCTACACGGGGATGAAACAAGTTATCGTGTGATTGA
AAGTGCCAAAACAGATACCTATTACTGGCAGTTTTGTACAGGAAAAGACAGTCAGCATCCCATCGTTTATTATCACCACGACGAAAGTCGTGCTGGAGAT
GTTCCGAAAACTTTTCTCAAGGAGTTTACAGGGTATTTACACTGTGACGGCTATAGCGGGTATAATGCCGTGGAAAGTGTACGACTTGTCTATTGTTTCG
CGCATGTTCGGCGGAAATTTTTCGAAGCAATTCCAAAAGGAAAGAAAAACACGGATATTCCCGCAGCTCAAGCAGTCAAACAATTGGATAAATGGTTTGT
CCTAGAAAAGAAATGGAAAGACTTTTCTCCTGAGAAGCGATTAAGCTGTCGGCAACAAGAACTGCGTCCATTATTTATTGCTTTTTATGAGTGGATGGCA
ACGATAGATCCTGTTGCAAAATCCAAGTTGGATGCAGCCGTTCAATATGCGTGTAAACTACGAAGTGGTTTTGAACCTATTTTTGAAGATGGTCGTTTAG
AACTCACAAATAATCGTGCGGAGCGAAATATCAAAGAGCTGGTGATAGGACGGAAAAATTGGTTGCATTCCACAAGTCTTGAAGGAGCACGAACTTCAGG
AATCATTCTGAGTGTATATAAAACAGCAGAACTTAACGGATTGAATCCTGTAAAGTATCTAGAGTTTCTATTTGATAAGATACCAAATCTTCCCGTACTT
TCAGCAGAAACTTTAGATCAACTGTTGCCTTGGAACAAGGATGTACAACAACATTTTTCTCGTAACTAAAAACGCCAAACATTCCCGATTCTATTCTATA
GGTCGGGTTATTTGACGTACAGGTACTGTTCTATGGGGCGCTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 195 bp | 64 aa | 83 | 277 | + | No |
AG : IS66 TnpA
ORF sequence :
MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNYILQAILKELLSNAL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 354 bp | 117 aa | 267 | 620 | + | No |
AG : IS66 TnpB
ORF sequence :
MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAVFLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEG
LSIDQPRAILPGKKGVF
LSIDQPRAILPGKKGVF
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1608 bp | 515 aa | 662 | 2269 | + | No |
Chemistry : DDE
ORF sequence :
METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTNKLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKH
LPITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPA
YRQENYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLK
EFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAK
SKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQ
LLPWNKDVQQHFSRN
LPITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPA
YRQENYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLK
EFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAK
SKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQ
LLPWNKDVQQHFSRN
Blast result :
Comments
ISEfa18 is 57% (transposase) aa similar to ISLhe66.
References
1] Moutusee ISlam (2020) Direct submission.
2] Wagner,T.M., Janice,J., Sivertsen,A., Sjogren,I., Sundsfjord,A. and Hegstad,K.(2020) Direct GenBank submission.
2] Wagner,T.M., Janice,J., Sivertsen,A., Sjogren,I., Sundsfjord,A. and Hegstad,K.(2020) Direct GenBank submission.