ISEfa16
- Family IS110
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| WEFW01000004 | ND | Enterococcus faecium | Enterococcus faecium Enterococcus faecium VRECG20 4 |
DNA section
IS Length : 1765 bp
Ends
Left end : CGTAATATATATTTTGAAACGACTTCCATCGTTTCAGTCACCCCTTAGGGCTATTCCTTATTAAATAACTTTTAAACCTTGGCTATTGAATTTTGTTCCT
Right end : GAAAAAAATTTGATTTTGGTCGGGTCTAGTTCAGCGCGGTCTGATTTTTCAAAATCTAACTCATATTCTAAAATTTATATCTTGACTTATTACCATTAGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGATCTCCTTCT | CTTTCTCTTAGGT | 0 |
DNA sequence
CGTAATATATATTTTGAAACGACTTCCATCGTTTCAGTCACCCCTTAGGGCTATTCCTTATTAAATAACTTTTAAACCTTGGCTATTGAATTTTGTTCCT
GGATAATAGACTTATGGAAACGGTCTATCGGAACACTGCTAATTTCCTTTGTAGCTATGACTACAGCAGGAAGCCTGCATCCCGACTTATTGATTATCAT
GCCTCAAACTCATTAGCTGTACCGTTCACTTCTTAAACTATTTCTAGTAGGAAATAGGGTGGCTTTTAAGAAGTAAACAATTGAGTACTATTAAGCGAGA
CTGCGGTTATTCAATAAGATTCAGGGATAAACTTCAATAGCTAAGTATTCCAAAAAATTTATGAAAGGAGGACCTTCCATGAAATTATTTATTGGTTTAG
ACGTTAGTTCTCAAAAATTAGATACTTGTTTTTTAACAGACTCCAAAGAAATCTTGTTTGAAGGTTCACTTAGCAACGATTTAATCGGAGCCAGCGAAAT
CAAACAAAGAATTCTCGAATACCAAGAAACCTTTGGTTTCTACCAAATTGTCATAGGTATGGAATCAACTTCTGTTTATAGTTTCCACCCAGCAATGTTT
TTCTACTTAGATGAAGATTTGAAGCTATTACCTGTCGAAGTTACTGTAGAAAATCCTTATAGAATCAAACAGTATTCAAGAATGTTCGATCAGGATAAAA
CAGATAAAATTGATGCACAGATCATAGCTGATTATCTGCGAGTAGATCTCCATACATTGTCTCCTATTAAAGAAGAAGTTTATGTTGGACTTCAACGACT
AACAAGATCTCGTTATCAATTAGTGACACAAATGGTAGAAGCAAAACAACATTTTTTAGAAAATTTGACGTATAAATGTAATACTTTATCAAAAGAACTT
GCGAATGAAGATAATAAAACTTCCATATTCGGAGCTTCGATTATGAATCTACTTACAGAAGGCATTACCTTAGATGAATTTTCAGAAATGCCTCTGGAAG
CGGCCGTTGAATTACTTCAAAAGCTAGGAAAAGGTCGATTCAAAAATCCAGAGCAGCTTGTCAAAAGTATTCAAAAAGCTGTACGTAGTTCTTATCGTTT
AGGTAAAGTAACCAAGAATTCCATTGACCTTGTATTAGGTATAATCGCCAATGAAATCAAGTTTCTAAAAAAACAGATTAAAGAAATTGAAAAAGCCATT
ACTGCTTTACTTGAAACAATTCCAGAAGCCCAATGCTTATTAAGTGTCCCAGGATTAGGAGCTGTTTATACTGCGGGCTTAATCGCTGAAATTGGTCAAA
TCGAGCGATTTGATGATCAATCCAAATTAGCGAAGTATGCTGGTCTAACTTGGACTAAAAATCAGTCGGGAAACTCTCAATCTGAAAACACGCCACTAAA
TCGTTCTGGAAATCGCTATTTTCGTTACTACTTAATTGAAGCCACCAATCGAGTAAGAAGCACACTTCCAGAATTTAAAGAGTACTACCAAAAAAAGAGA
AATGAAGTACCTAAACACAAACATAAACGAGCGCTCGTCTTAACCGGAAGAAAATTTGTACGCTTGATCGATGCGCTACTACGCAACGGTCAATTCTATC
AGCCAGCGAAGGTGGTGATAGAAAAATAACGTAAAGTTATTTGCGAAAATCTTCGTGATCAACCCGAAAAAAATTTGATTTTGGTCGGGTCTAGTTCAGC
GCGGTCTGATTTTTCAAAATCTAACTCATATTCTAAAATTTATATCTTGACTTATTACCATTAGG
GGATAATAGACTTATGGAAACGGTCTATCGGAACACTGCTAATTTCCTTTGTAGCTATGACTACAGCAGGAAGCCTGCATCCCGACTTATTGATTATCAT
GCCTCAAACTCATTAGCTGTACCGTTCACTTCTTAAACTATTTCTAGTAGGAAATAGGGTGGCTTTTAAGAAGTAAACAATTGAGTACTATTAAGCGAGA
CTGCGGTTATTCAATAAGATTCAGGGATAAACTTCAATAGCTAAGTATTCCAAAAAATTTATGAAAGGAGGACCTTCCATGAAATTATTTATTGGTTTAG
ACGTTAGTTCTCAAAAATTAGATACTTGTTTTTTAACAGACTCCAAAGAAATCTTGTTTGAAGGTTCACTTAGCAACGATTTAATCGGAGCCAGCGAAAT
CAAACAAAGAATTCTCGAATACCAAGAAACCTTTGGTTTCTACCAAATTGTCATAGGTATGGAATCAACTTCTGTTTATAGTTTCCACCCAGCAATGTTT
TTCTACTTAGATGAAGATTTGAAGCTATTACCTGTCGAAGTTACTGTAGAAAATCCTTATAGAATCAAACAGTATTCAAGAATGTTCGATCAGGATAAAA
CAGATAAAATTGATGCACAGATCATAGCTGATTATCTGCGAGTAGATCTCCATACATTGTCTCCTATTAAAGAAGAAGTTTATGTTGGACTTCAACGACT
AACAAGATCTCGTTATCAATTAGTGACACAAATGGTAGAAGCAAAACAACATTTTTTAGAAAATTTGACGTATAAATGTAATACTTTATCAAAAGAACTT
GCGAATGAAGATAATAAAACTTCCATATTCGGAGCTTCGATTATGAATCTACTTACAGAAGGCATTACCTTAGATGAATTTTCAGAAATGCCTCTGGAAG
CGGCCGTTGAATTACTTCAAAAGCTAGGAAAAGGTCGATTCAAAAATCCAGAGCAGCTTGTCAAAAGTATTCAAAAAGCTGTACGTAGTTCTTATCGTTT
AGGTAAAGTAACCAAGAATTCCATTGACCTTGTATTAGGTATAATCGCCAATGAAATCAAGTTTCTAAAAAAACAGATTAAAGAAATTGAAAAAGCCATT
ACTGCTTTACTTGAAACAATTCCAGAAGCCCAATGCTTATTAAGTGTCCCAGGATTAGGAGCTGTTTATACTGCGGGCTTAATCGCTGAAATTGGTCAAA
TCGAGCGATTTGATGATCAATCCAAATTAGCGAAGTATGCTGGTCTAACTTGGACTAAAAATCAGTCGGGAAACTCTCAATCTGAAAACACGCCACTAAA
TCGTTCTGGAAATCGCTATTTTCGTTACTACTTAATTGAAGCCACCAATCGAGTAAGAAGCACACTTCCAGAATTTAAAGAGTACTACCAAAAAAAGAGA
AATGAAGTACCTAAACACAAACATAAACGAGCGCTCGTCTTAACCGGAAGAAAATTTGTACGCTTGATCGATGCGCTACTACGCAACGGTCAATTCTATC
AGCCAGCGAAGGTGGTGATAGAAAAATAACGTAAAGTTATTTGCGAAAATCTTCGTGATCAACCCGAAAAAAATTTGATTTTGGTCGGGTCTAGTTCAGC
GCGGTCTGATTTTTCAAAATCTAACTCATATTCTAAAATTTATATCTTGACTTATTACCATTAGG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1251 bp | 416 aa | 379 | 1629 | + | No |
Chemistry : DEDD
ORF sequence :
MKLFIGLDVSSQKLDTCFLTDSKEILFEGSLSNDLIGASEIKQRILEYQETFGFYQIVIGMESTSVYSFHPAMFFYLDEDLKLLPVEVTVENPYRIKQYS
RMFDQDKTDKIDAQIIADYLRVDLHTLSPIKEEVYVGLQRLTRSRYQLVTQMVEAKQHFLENLTYKCNTLSKELANEDNKTSIFGASIMNLLTEGITLDE
FSEMPLEAAVELLQKLGKGRFKNPEQLVKSIQKAVRSSYRLGKVTKNSIDLVLGIIANEIKFLKKQIKEIEKAITALLETIPEAQCLLSVPGLGAVYTAG
LIAEIGQIERFDDQSKLAKYAGLTWTKNQSGNSQSENTPLNRSGNRYFRYYLIEATNRVRSTLPEFKEYYQKKRNEVPKHKHKRALVLTGRKFVRLIDAL
LRNGQFYQPAKVVIEK
RMFDQDKTDKIDAQIIADYLRVDLHTLSPIKEEVYVGLQRLTRSRYQLVTQMVEAKQHFLENLTYKCNTLSKELANEDNKTSIFGASIMNLLTEGITLDE
FSEMPLEAAVELLQKLGKGRFKNPEQLVKSIQKAVRSSYRLGKVTKNSIDLVLGIIANEIKFLKKQIKEIEKAITALLETIPEAQCLLSVPGLGAVYTAG
LIAEIGQIERFDDQSKLAKYAGLTWTKNQSGNSQSENTPLNRSGNRYFRYYLIEATNRVRSTLPEFKEYYQKKRNEVPKHKHKRALVLTGRKFVRLIDAL
LRNGQFYQPAKVVIEK
Blast result :
Comments
ISEfa16 is 74% aa similar to ISSpn10.
There is an empty site in Enterococcus faecium isolate E7240 genome assembly, plasmid: 3 (Accession number : LR135309.1), position 23761-23762.
There is an empty site in Enterococcus faecium isolate E7240 genome assembly, plasmid: 3 (Accession number : LR135309.1), position 23761-23762.
References
1] Moutusee Islam (2020) Direct submission.
2] Chilambi,G.S., Nordstrom,H.R., Evans,D.R., Ferrolino,J., Hayden,R.T., Maron,G.M., Vo,A.N., Gilmore,M.S., Wolf,J., Rosch,J.W. and Van Tyne,D. (2019) Direct GenBank submission.
2] Chilambi,G.S., Nordstrom,H.R., Evans,D.R., Ferrolino,J., Hayden,R.T., Maron,G.M., Vo,A.N., Gilmore,M.S., Wolf,J., Rosch,J.W. and Van Tyne,D. (2019) Direct GenBank submission.