ISCrsa1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Cronobacter sakazakii | Cronobacter sakazakii Escherichia coli O10:H32 NMBU-W12E19 Shigella sonnei 866 plasmid p866 |
DNA section
IS Length : 1613 bp
Ends
IR Length : 20/29
IRL : CCCCCTCTGTCAGGATAGTTGTCACCCCCATCGAAAATACTAATGACAAG
IRR : CGGTAAGTGTCAAGGTAGTTGGCACCCCCGGTTGATTTAGGCTGCCTGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCCCCTCTGTCAGGATAGTTGTCACCCCC | GGGGGTGCCAACTACCTTGACACTTACCG | 0 | |
| TTCTGCTCAA | TAT | ATGATAGAA | 3 |
DNA sequence
CCCCCTCTGTCAGGATAGTTGTCACCCCCATCGAAAATACTAATGACAAGATGGGAACAGATCGTGAAACGAATTTCACCAGAACGTAAATCGGCTGCAC
TGGCTAAATTACTACCGCCTTACAACATGACCGTCACCGCAGTTGCGCAGATGGAAGGGATATCGGAAGCTACCCTTTATTACTGGCGTAACAAGGCGAA
AGCAGAGGGAATACCGGTGCCGGGTGCAAACAAAACTACAGACCAATGGTCAGCCGAAGCCCGGCTGGCTGTGATTATCGAAACAGCTACACTCAGCGAA
ACAGAACTCGCGCAATACTGCCGTAAAAAAGGGCTTTACCTGGAACAGATTAGTCAGTGGAAACAAGGCTTTTTGCAGGTGTCTTCGCCCACTGACAAAG
CGGCGCTCAAACAAAGCCAGAAAGAAGTTAAACAACTAAAACGCGAACTGCTTCGTAAGGAAAAGGCGCTGGCGGAGGCGGCAGCGATACTGGTGCTGCG
AAAGCAGTTGCGGGATTTTTACGGGGAAACCGACGAGGACGACTGACGCCCGGAACAGAGCGGCAGGCACTTATCGGTTACGTGCGGGACGCGATGGCGT
CAGGAGCTCGGCTGTCAATAGCGCTGACGGAAGCGGGATTAAGTGAACGGACGTGGCGACGCTGGCAAACGTCGTGGGAAGACAAGCGTGTCAGCACGGT
CAGGCCCTCCCCGGTGAACCAGTTAAGTGAGCAAGAAGAGCAACAGATACTGGCAGTGTGCCATCAGCCAGACTACGCCAGCCTCCCCCCGTCACAAATC
GTACCGGCGTTGGCGGATAAGGGGATTTATCTGGCAAGCGAGTCAACCTTTTACCGGGTACTGCGTCGTCATGGAGAGGTGCATCGCCGGGGACGGCAAC
GGGCAGCGCAGAAAGTCACCCCGGCTACGAGTTGGCAGGCCAGCGGGCCGAATGAGGTGTGGACATGGGACATTACATGGGTTCCGTCAACCGTAAAGGG
GCGCTGGTTTTACCTGTATCTGGTGGAAGACCTCTACAGCCGAAAAATCGTGGGATATGAAGTGCATGAAACTGAAAGTGGTGAGCAGGCGGCAGCCCTG
ATACAACGCACGGTCCTTCGGGAAGGATGCTGGCAAAACCCGCCGGTACTGCATGCGGACAACGGCGCGGCGATGAAGTCACAGACGTTGCAAATGAAGC
TACACGAGCTGGCGATAACCCCCTCTCACAGCCGGCCGGGAGTGAGTAACGATAATGCGTATGTGGAGTCGCTGTTCCGGACACTGAAATATGTCCCGCA
GTGGCCGTCATCAGGGTTCGGCAAGCTGGAGGAGGCGCGTGAATGGATAGAGGTTTTTGCTCAGTGGTATAACGAAATGCACAGACATAGCGGCATCAGG
TATGTGACGCCGGGTCAGCGACATCGTGGAGAAGACGGTGTGTTGCTGAAAAATCGGGATGAGGTGTACAAAAAGGCAAAAGCGGAACGGCCTGAACGCT
GGTCTGGCAGAACACGAAACTGGCATCCGGTAGGCAAGGTAATGCTGAATCCAGAACGGGAAAAACAGGCAGCCTAAATCAACCGGGGGTGCCAACTACC
TTGACACTTACCG
TGGCTAAATTACTACCGCCTTACAACATGACCGTCACCGCAGTTGCGCAGATGGAAGGGATATCGGAAGCTACCCTTTATTACTGGCGTAACAAGGCGAA
AGCAGAGGGAATACCGGTGCCGGGTGCAAACAAAACTACAGACCAATGGTCAGCCGAAGCCCGGCTGGCTGTGATTATCGAAACAGCTACACTCAGCGAA
ACAGAACTCGCGCAATACTGCCGTAAAAAAGGGCTTTACCTGGAACAGATTAGTCAGTGGAAACAAGGCTTTTTGCAGGTGTCTTCGCCCACTGACAAAG
CGGCGCTCAAACAAAGCCAGAAAGAAGTTAAACAACTAAAACGCGAACTGCTTCGTAAGGAAAAGGCGCTGGCGGAGGCGGCAGCGATACTGGTGCTGCG
AAAGCAGTTGCGGGATTTTTACGGGGAAACCGACGAGGACGACTGACGCCCGGAACAGAGCGGCAGGCACTTATCGGTTACGTGCGGGACGCGATGGCGT
CAGGAGCTCGGCTGTCAATAGCGCTGACGGAAGCGGGATTAAGTGAACGGACGTGGCGACGCTGGCAAACGTCGTGGGAAGACAAGCGTGTCAGCACGGT
CAGGCCCTCCCCGGTGAACCAGTTAAGTGAGCAAGAAGAGCAACAGATACTGGCAGTGTGCCATCAGCCAGACTACGCCAGCCTCCCCCCGTCACAAATC
GTACCGGCGTTGGCGGATAAGGGGATTTATCTGGCAAGCGAGTCAACCTTTTACCGGGTACTGCGTCGTCATGGAGAGGTGCATCGCCGGGGACGGCAAC
GGGCAGCGCAGAAAGTCACCCCGGCTACGAGTTGGCAGGCCAGCGGGCCGAATGAGGTGTGGACATGGGACATTACATGGGTTCCGTCAACCGTAAAGGG
GCGCTGGTTTTACCTGTATCTGGTGGAAGACCTCTACAGCCGAAAAATCGTGGGATATGAAGTGCATGAAACTGAAAGTGGTGAGCAGGCGGCAGCCCTG
ATACAACGCACGGTCCTTCGGGAAGGATGCTGGCAAAACCCGCCGGTACTGCATGCGGACAACGGCGCGGCGATGAAGTCACAGACGTTGCAAATGAAGC
TACACGAGCTGGCGATAACCCCCTCTCACAGCCGGCCGGGAGTGAGTAACGATAATGCGTATGTGGAGTCGCTGTTCCGGACACTGAAATATGTCCCGCA
GTGGCCGTCATCAGGGTTCGGCAAGCTGGAGGAGGCGCGTGAATGGATAGAGGTTTTTGCTCAGTGGTATAACGAAATGCACAGACATAGCGGCATCAGG
TATGTGACGCCGGGTCAGCGACATCGTGGAGAAGACGGTGTGTTGCTGAAAAATCGGGATGAGGTGTACAAAAAGGCAAAAGCGGAACGGCCTGAACGCT
GGTCTGGCAGAACACGAAACTGGCATCCGGTAGGCAAGGTAATGCTGAATCCAGAACGGGAAAAACAGGCAGCCTAAATCAACCGGGGGTGCCAACTACC
TTGACACTTACCG
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 503 bp | 167 aa | 43 | 545 | + | No |
Description : First part of the transposase
ORF sequence :
MTRWEQIVKRISPERKSAALAKLLPPYNMTVTAVAQMEGISEATLYYWRNKAKAEGIPVPGANKTTDQWSAEARLAVIIETATLSETELAQYCRKKGLYL
EQISQWKQGFLQVSSPTDKAALKQSQKEVKQLKRELLRKEKALAEAAAILVLRKQLRDFYGETDEDD
EQISQWKQGFLQVSSPTDKAALKQSQKEVKQLKRELLRKEKALAEAAAILVLRKQLRDFYGETDEDD
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1119 bp | 372 aa | 459 | 1577 | + | No |
Description : Second part of the transposase
ORF sequence :
GKGAGGGGSDTGAAKAVAGFLRGNRRGRLTPGTERQALIGYVRDAMASGARLSIALTEAGLSERTWRRWQTSWEDKRVSTVRPSPVNQLSEQEEQQILAV
CHQPDYASLPPSQIVPALADKGIYLASESTFYRVLRRHGEVHRRGRQRAAQKVTPATSWQASGPNEVWTWDITWVPSTVKGRWFYLYLVEDLYSRKIVGY
EVHETESGEQAAALIQRTVLREGCWQNPPVLHADNGAAMKSQTLQMKLHELAITPSHSRPGVSNDNAYVESLFRTLKYVPQWPSSGFGKLEEAREWIEVF
AQWYNEMHRHSGIRYVTPGQRHRGEDGVLLKNRDEVYKKAKAERPERWSGRTRNWHPVGKVMLNPEREKQAA
CHQPDYASLPPSQIVPALADKGIYLASESTFYRVLRRHGEVHRRGRQRAAQKVTPATSWQASGPNEVWTWDITWVPSTVKGRWFYLYLVEDLYSRKIVGY
EVHETESGEQAAALIQRTVLREGCWQNPPVLHADNGAAMKSQTLQMKLHELAITPSHSRPGVSNDNAYVESLFRTLKYVPQWPSSGFGKLEEAREWIEVF
AQWYNEMHRHSGIRYVTPGQRHRGEDGVLLKNRDEVYKKAKAERPERWSGRTRNWHPVGKVMLNPEREKQAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1535 bp | 511 aa | 43 | 1577 | + | Yes |
Chemistry : DDE
ORF sequence :
MTRWEQIVKRISPERKSAALAKLLPPYNMTVTAVAQMEGISEATLYYWRNKAKAEGIPVPGANKTTDQWSAEARLAVIIETATLSETELAQYCRKKGLYL
EQISQWKQGFLQVSSPTDKAALKQSQKEVKQLKRELLRKEKALAEAAAILVLRKQLRDFLRGNRRGRLTPGTERQALIGYVRDAMASGARLSIALTEAGL
SERTWRRWQTSWEDKRVSTVRPSPVNQLSEQEEQQILAVCHQPDYASLPPSQIVPALADKGIYLASESTFYRVLRRHGEVHRRGRQRAAQKVTPATSWQA
SGPNEVWTWDITWVPSTVKGRWFYLYLVEDLYSRKIVGYEVHETESGEQAAALIQRTVLREGCWQNPPVLHADNGAAMKSQTLQMKLHELAITPSHSRPG
VSNDNAYVESLFRTLKYVPQWPSSGFGKLEEAREWIEVFAQWYNEMHRHSGIRYVTPGQRHRGEDGVLLKNRDEVYKKAKAERPERWSGRTRNWHPVGKV
MLNPEREKQAA
EQISQWKQGFLQVSSPTDKAALKQSQKEVKQLKRELLRKEKALAEAAAILVLRKQLRDFLRGNRRGRLTPGTERQALIGYVRDAMASGARLSIALTEAGL
SERTWRRWQTSWEDKRVSTVRPSPVNQLSEQEEQQILAVCHQPDYASLPPSQIVPALADKGIYLASESTFYRVLRRHGEVHRRGRQRAAQKVTPATSWQA
SGPNEVWTWDITWVPSTVKGRWFYLYLVEDLYSRKIVGYEVHETESGEQAAALIQRTVLREGCWQNPPVLHADNGAAMKSQTLQMKLHELAITPSHSRPG
VSNDNAYVESLFRTLKYVPQWPSSGFGKLEEAREWIEVFAQWYNEMHRHSGIRYVTPGQRHRGEDGVLLKNRDEVYKKAKAERPERWSGRTRNWHPVGKV
MLNPEREKQAA
Blast result :
Comments
ISCrsa1 is 74% (ORFAB) aa similar to ISPa74.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
Accession number of Escherichia coli O10:H32 strain NMBU-W12E19 sequence copy : CP042892.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
Accession number of Escherichia coli O10:H32 strain NMBU-W12E19 sequence copy : CP042892.
References
1] Zhiyong Zong (2020) Direct submission.
2] Finton,M.D., Meisal,R., Porcellato,D., Brandal,L.T. and Lindstedt,B.-A. (2019) Direct GenBank submission.
2] Finton,M.D., Meisal,R., Porcellato,D., Brandal,L.T. and Lindstedt,B.-A. (2019) Direct GenBank submission.