ISH23
- Family IS1595
- Group ISH4
Isoform ISH50Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| Y | Halobacterium salinarum | Halobacterium tunesiensis A2 Halobacterium salinarum PHH1 plasmid pHH1 Halobacterium salinarum NRC34020 Halobacterium salinarum NRL Halobacterium salinarum GN101 |
DNA section
IS Length : 1000 bp
Ends
IR Length : 23/29
IRL :
IRR :
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTCGTAGAGT | TACACACAT | ATCCTCGTTA | 9 |
| NNNNAGATAT | TCTTGGTTA | AGTTTGNNNN | 9 |
DNA sequence
Protein section
ORF number : 0
Comments
ISH23 is very similar (but not identical) to ISH50 (Pfeifer et al., 1984): although two restriction sites are different between the two elements, their first and last 200 bp were found to identical (Pfeifer, 1993). ISH23 has been isolated through its inactivation of the bacteriorhodopsin (BR, bop) gene. There are 2 ISH23/ISH50 copies in the Halobacterium salinarum NRC817 chromosome (Pfeifer et al., 1986).
References
1] Pfeifer, F., Betlach, M., Martienssen, R., Friedman, J., and Boyer, H.W. (1983) Mol. Gen. Genet. 191, 182-188.
2] Pfeifer, F., Friedman, J., Boyer, H.W., and Betlach, M. (1984) Nucl. Acids Res. 12, 2489-2497.
3] Pfeifer, F. (1986) System. Appl. Microbiol. 7, 36-40.
4] DasSarma, S. (1989) Can. J. Microbiol. 35, 65-72.
5] Pfeifer, F., Blaseio, U., and Horne, M. (1989) Can. J. Microbiol. 35, 96-100.
6] Pfeifer, F., and Englert, C. (1992) J. Bioenergetics and Biomembranes 24, 577-585.<br>7] Pfeifer, F. (1993) Personal communication.
2] Pfeifer, F., Friedman, J., Boyer, H.W., and Betlach, M. (1984) Nucl. Acids Res. 12, 2489-2497.
3] Pfeifer, F. (1986) System. Appl. Microbiol. 7, 36-40.
4] DasSarma, S. (1989) Can. J. Microbiol. 35, 65-72.
5] Pfeifer, F., Blaseio, U., and Horne, M. (1989) Can. J. Microbiol. 35, 96-100.
6] Pfeifer, F., and Englert, C. (1992) J. Bioenergetics and Biomembranes 24, 577-585.<br>7] Pfeifer, F. (1993) Personal communication.