ISfinder Mirror Site

ISFop2

Family IS5
Group IS427

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP022377	ND	Francisella opportunistica	Francisella sp. MA067296 Francisella opportunistica Francisella opportunistica PA05-1188 Francisella sp. PA05-1188

DNA section

IS Length : 851 bp

Ends

IR Length : 20

IRL : GGTTCCATGAACAAAAAAATTGAATAAAGCAGTTAAGTATATTTAAATAG
IRR : GGTTCCATGAACAAAAAAATCATCTAAGCCAAATAAAGGTGCTTGCTAAT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CAAAATTTAT	TA	ACTATAGCAT	2
GTTTTATAAC	TA	ACTAATATAC	2
CAATTTAGAT	CA	ATTGGGTGAA	2
AATAATAATA	TA	AGTAGTGAAA	2
ATAATGATAA	TA	ATATTAAATA	2
AGATACTCTA	TA	GCCTCATCTT	2
ATCCAATAAA	ta	ATTTACGCAT	2
TCTTTTTATC	ta	ATCACTCAAT	2
GATTGTTTAG	TA	ATATAAAGAC	2
AACAATAAAA	ta	ATAAAATATT	2
AGTGATTGAT	ta	ATTTATACTT	2

DNA sequence

GGTTCCATGAACAAAAAAATTGAATAAAGCAGTTAAGTATATTTAAATAGTTTTGTGAAACAAAAAAACATACTTAACATATATGGAATATTACATATCA
GAAACAAATTGGTCAACTATTTTAACTTTTCTAACAGCTCAAAAAGGATTGCATACAAAAGATGTAGTTAAGCTTAGAAGATTCATAGAAGCAGTGTTTT
TTATCTTAAAAACAGGAGCTCAGTGGAAATATCTTCACAAGGATTATGGTAATAGTAGAGCCATCCATAAGCGTTATAAATACTGGGCTGACAAAGGTGT
TTGGAATAATCTAATGACTTATGTTTCAGACATTGATTCACAACAGTTCATGATAGATTCACTATCTGTTAAAGCACATGCGTGTGCTAGTGGTTATGAG
ATAAATGGTAATGAAATCAATGCTTTGGGTAGAAGTGTGGGTGGCTTAACAACAAAGATTCATACTCTTACAGATGCTTTAGGAAATCCCGTTAGATTTA
TAATAACTGCTGGTAATGTCCATGATATTGTACCCTCACAACAACTCTTAGAAGGAATAACAAATGCTTATATTCTTGCTGATAAGGCATATTTCTCTGA
AGAAAATATAAAATTCCTTGAGGAAAATAAAAATAAACCAGTCATTCCTGCTAGAGAAAACTATACCAAAGGCCATAATGTTGACTGGCATATTTATAAG
GAAAGACATTTGATAGAGAACTTTTTCTCTAAAATTAAGCATTTTAGAAGGGTTTTCTCTAGGTTTGATAAGACTTGTTCTGCTTTCCTCGGCTTTATAG
CATTAGCAAGCACCTTTATTTGGCTTAGATGATTTTTTTGTTCATGGAACC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
750 bp	249 aa	83	832	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MEYYISETNWSTILTFLTAQKGLHTKDVVKLRRFIEAVFFILKTGAQWKYLHKDYGNSRAIHKRYKYWADKGVWNNLMTYVSDIDSQQFMIDSLSVKAHA
CASGYEINGNEINALGRSVGGLTTKIHTLTDALGNPVRFIITAGNVHDIVPSQQLLEGITNAYILADKAYFSEENIKFLEENKNKPVIPARENYTKGHNV
DWHIYKERHLIENFFSKIKHFRRVFSRFDKTCSAFLGFIALASTFIWLR

Blast result :

Comments

ISFop2 is 70% aa similar to ISLpn10.

References

1] Elizabeth Dietrich (2018) Direct submission.