ISPa107
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| LT969519 | ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa RW109 |
DNA section
IS Length : 1241 bp
Ends
IR Length : 21/30
IRL : TGGCCTAATGATCCCGGACACCTCTTAAGGGCGATATGATTCGCCCAATC
IRR : TGGTCAAGTATTTCCGGACACCGGTTACGGTGATCAAGCCGCCTGCTGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATGAGCAGGCT | GAAC | AGGCCGGCACCTATGA | 4 |
DNA sequence
TGGCCTAATGATCCCGGACACCTCTTAAGGGCGATATGATTCGCCCAATCAGGAGGTTCCATGCAAGAACGAAAGACCTACACCCGCGAGTTCAAGCAAC
GTGCTGCCAGCATGGTCCTTGATGACCACTGCTCGATTCCCGACGTCTGCGCCTCAATGGACGTCGGCCCTACAGCCCTGCGCCGCTGGGTTGATCAGGT
GCGTAAAGAGCGACACAAGGGCCAGCCAGTAGCCGGTACCAAAGCGATCAGCGACGAACAGCGCGAGCTCCAGCAGCTGCGAGCCAAGATCAAGCGCCTG
GAGACCGAGGCTGAAATCTTAAAAAAGGCTACGGCTCTCTTGATGTCGGATCCCGATCGTTTTTCCTGATTGAGGAACTGAGAGAGTCAGGCTCGTATCC
GACCAGCCTTCTTTGCAGTGCGCTGAGCGTATCCCGTAGCGCGTTTTATGACTGGCTTCGCCGTCGTTCAACACCGAATACCAAGCGTGACGAACTCAGA
GAGAAAGTAGTTCAGCTGCACGTCGAAAGCAGGGGAAGCGCGGGCGCGAGAATGATCTCTGAGTACCTGAAGGCCCAGCAGCTCAAGGTGGGACGGTATC
TGGCCGGAAAACTCATGGAGGAGGCAAATCTGGAAAGCAAGCAGCGTCGTCCTCATCGGAATCGCTCCAAAGGTGTTGAAGCGTTTGTGGCCAAGAACCT
GCTCGAACGCAACTTTGAGCCAACAGCAATTAACCAGGTCTGGTGCGGTGACGTGACCAGTTTGATGGTCGGGAAGCGCTGGTATCACCTGGCAGTAGTG
ATTGACTTGTTTGCGCGTCGGATCGTTGGCTGGGCGTTTTCCCTGATCAACGACGCCAACTTGGTTAGCAAGGCACTGAGGATGGCAGTAGGGGTTAGGG
GCAAGCATACAGGCTTGATGTTTCACTCGGATCAAGGCTGCCAATACACCAGCCAGCGCTTCCAGTCCGAGCTGCTTGAGCATGGCATAACGCAGAGCAT
GAGTCGCAGGGGACAATGCTGGGACAACGCACCAACAGAGCGTTTCTTTGGAACGCTCAAGTCAGAATGGGTGCCGCCCAAGGGCTACAAGGAAATTGAA
GAAGCCAAGCAGGACATGACCAGCTTCTTCATGCGATACAACCGAATCAGGCTCCACAGCTACAACGACTACCTGTCACCAATAGCTATGGAGCAGCAGG
CGGCTTGATCACCGTAACCGGTGTCCGGAAATACTTGACCA
GTGCTGCCAGCATGGTCCTTGATGACCACTGCTCGATTCCCGACGTCTGCGCCTCAATGGACGTCGGCCCTACAGCCCTGCGCCGCTGGGTTGATCAGGT
GCGTAAAGAGCGACACAAGGGCCAGCCAGTAGCCGGTACCAAAGCGATCAGCGACGAACAGCGCGAGCTCCAGCAGCTGCGAGCCAAGATCAAGCGCCTG
GAGACCGAGGCTGAAATCTTAAAAAAGGCTACGGCTCTCTTGATGTCGGATCCCGATCGTTTTTCCTGATTGAGGAACTGAGAGAGTCAGGCTCGTATCC
GACCAGCCTTCTTTGCAGTGCGCTGAGCGTATCCCGTAGCGCGTTTTATGACTGGCTTCGCCGTCGTTCAACACCGAATACCAAGCGTGACGAACTCAGA
GAGAAAGTAGTTCAGCTGCACGTCGAAAGCAGGGGAAGCGCGGGCGCGAGAATGATCTCTGAGTACCTGAAGGCCCAGCAGCTCAAGGTGGGACGGTATC
TGGCCGGAAAACTCATGGAGGAGGCAAATCTGGAAAGCAAGCAGCGTCGTCCTCATCGGAATCGCTCCAAAGGTGTTGAAGCGTTTGTGGCCAAGAACCT
GCTCGAACGCAACTTTGAGCCAACAGCAATTAACCAGGTCTGGTGCGGTGACGTGACCAGTTTGATGGTCGGGAAGCGCTGGTATCACCTGGCAGTAGTG
ATTGACTTGTTTGCGCGTCGGATCGTTGGCTGGGCGTTTTCCCTGATCAACGACGCCAACTTGGTTAGCAAGGCACTGAGGATGGCAGTAGGGGTTAGGG
GCAAGCATACAGGCTTGATGTTTCACTCGGATCAAGGCTGCCAATACACCAGCCAGCGCTTCCAGTCCGAGCTGCTTGAGCATGGCATAACGCAGAGCAT
GAGTCGCAGGGGACAATGCTGGGACAACGCACCAACAGAGCGTTTCTTTGGAACGCTCAAGTCAGAATGGGTGCCGCCCAAGGGCTACAAGGAAATTGAA
GAAGCCAAGCAGGACATGACCAGCTTCTTCATGCGATACAACCGAATCAGGCTCCACAGCTACAACGACTACCTGTCACCAATAGCTATGGAGCAGCAGG
CGGCTTGATCACCGTAACCGGTGTCCGGAAATACTTGACCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 309 bp | 102 aa | 61 | 369 | + | No |
Description : First part of the transposase
ORF sequence :
MQERKTYTREFKQRAASMVLDDHCSIPDVCASMDVGPTALRRWVDQVRKERHKGQPVAGTKAISDEQRELQQLRAKIKRLETEAEILKKATALLMSDPDR
FS
FS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 894 bp | 297 aa | 315 | 1208 | + | No |
Description : Second part of the transposase
ORF sequence :
NLKKGYGSLDVGSRSFFLIEELRESGSYPTSLLCSALSVSRSAFYDWLRRRSTPNTKRDELREKVVQLHVESRGSAGARMISEYLKAQQLKVGRYLAGKL
MEEANLESKQRRPHRNRSKGVEAFVAKNLLERNFEPTAINQVWCGDVTSLMVGKRWYHLAVVIDLFARRIVGWAFSLINDANLVSKALRMAVGVRGKHTG
LMFHSDQGCQYTSQRFQSELLEHGITQSMSRRGQCWDNAPTERFFGTLKSEWVPPKGYKEIEEAKQDMTSFFMRYNRIRLHSYNDYLSPIAMEQQAA
MEEANLESKQRRPHRNRSKGVEAFVAKNLLERNFEPTAINQVWCGDVTSLMVGKRWYHLAVVIDLFARRIVGWAFSLINDANLVSKALRMAVGVRGKHTG
LMFHSDQGCQYTSQRFQSELLEHGITQSMSRRGQCWDNAPTERFFGTLKSEWVPPKGYKEIEEAKQDMTSFFMRYNRIRLHSYNDYLSPIAMEQQAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1148 bp | 382 aa | 61 | 1208 | - | No |
Chemistry : DDE
ORF sequence :
MQERKTYTREFKQRAASMVLDDHCSIPDVCASMDVGPTALRRWVDQVRKERHKGQPVAGTKAISDEQRELQQLRAKIKRLETEAEILKKGYGSLDVGSRS
FFLIEELRESGSYPTSLLCSALSVSRSAFYDWLRRRSTPNTKRDELREKVVQLHVESRGSAGARMISEYLKAQQLKVGRYLAGKLMEEANLESKQRRPHR
NRSKGVEAFVAKNLLERNFEPTAINQVWCGDVTSLMVGKRWYHLAVVIDLFARRIVGWAFSLINDANLVSKALRMAVGVRGKHTGLMFHSDQGCQYTSQR
FQSELLEHGITQSMSRRGQCWDNAPTERFFGTLKSEWVPPKGYKEIEEAKQDMTSFFMRYNRIRLHSYNDYLSPIAMEQQAA
FFLIEELRESGSYPTSLLCSALSVSRSAFYDWLRRRSTPNTKRDELREKVVQLHVESRGSAGARMISEYLKAQQLKVGRYLAGKLMEEANLESKQRRPHR
NRSKGVEAFVAKNLLERNFEPTAINQVWCGDVTSLMVGKRWYHLAVVIDLFARRIVGWAFSLINDANLVSKALRMAVGVRGKHTGLMFHSDQGCQYTSQR
FQSELLEHGITQSMSRRGQCWDNAPTERFFGTLKSEWVPPKGYKEIEEAKQDMTSFFMRYNRIRLHSYNDYLSPIAMEQQAA
Blast result :
Comments
ISPa107 is 96% aa similar to ISPa98.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Xiaoyuan Jiang (2019) Direct submission.
2] Green,A. (2017) Direct GenBank submission.
2] Green,A. (2017) Direct GenBank submission.