ISAlw27
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP019143.1 | ND | Acinetobacter lwoffii | Acinetobacter lwoffii Acinetobacter lwoffii ZS207 Acinetobacter lwoffii EK30A pALWEK1.1 |
DNA section
IS Length : 1282 bp
Ends
IR Length : 28
IRL : AGAGATGGCTCCGTAAATTTGAACAACTCCTATAAGTGATATTTTGCTCC
IRR : AGAGATGGTCCCACTTGTTTGAACAACTAAAAGCTTATTTATAAGTGATA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| catggttaagcctatgagtc | atta | ctaggatgtgacattctgca | 4 |
DNA sequence
AGAGATGGCTCCGTAAATTTGAACAACTCCTATAAGTGATATTTTGCTCCCCAAATGATGTTATAAACATCGATATATGGAGTATTTTATGGCACGTAGA
CCAAGAAGAAATCATTCAAATGACTTTAAAGCTAAGGTAGCGCTTGCTGCGATTAAAGCAGAAAAAACACTTGCTGAATTGAGTGCTGAATTTGATGTTC
ATCAAAACCAAATTATCGACTGGAAAAATCAATTGCTTTCAGCTTCCTCGCAAGCCTTCGATCAATCAAAAGCTCCAGCAGAACCGCCCATTGATCTAAA
AAAACTACATGCAAAAATCGGTGAACAGGCATTAGAAATTGATTTTTTAGAAGGTGTGTTGAAGAAACTGGGCCGCTTCAACCACAAAAGTTAATCGACG
ACTCACTTCAGATTTCAGTATCTAAGCAAGCTAAGCTACTGAAAGTCTCCCGTGGTTGTTATTACTATCGCCCAAAACCTGTGAGTGCATCAGATCTGAA
GCTGATGCGCTGTATTGATGAATTACATATGCAATACCCTTTTGCAGGTAGCCGTATGATGCGTGATTTATTGAACCGTCAAGGACATCATATAGGACGA
CGTCATACACGTACTTTAATGAAGAAAATGGGCATTAATGCGTTATATCGTAAACCAAATCTAAGTCAGGCCAATCAAGCTCACCGTAAATATCCATATC
TGCTCAAAGGATTGAATATTCAACGGAGTAATCAAGTGTGGGCAACGGATATAACGTATATCCCTATGGCAAAAGGCTTTGTCTACTTATGTGCTGTGAT
TGATTGGCACAGCCGTAAGGTGCTTGCCCATAGCGTATCGATTAGTATGGAGGTTGCATTTTGTATAGAAACATTAAATGAAGCTATTGAAAAATATGGC
CGACCTGAAATCTTTAATACAGACCAAGGCAGTCAATTTACCAGTGATGCATTTATTGAAGTGTTAAAATCAAATGACATCCAAATCAGCATGGATGGTA
AAGGTCGTTGGGTTGATAATGTGATGATTGAACGATTATGGCGGAGCGTTAAATACGAGGAGGTTTATCTCAAAGCCTACAGCAATGTTTTTGATGCGAA
AAAGCAATTAAATGCCTATTTTGAATTTTATAATTTGAAACGACCTCATTCGAGTCTGGACAAAATGACTCCAGATGAGTTTTACTATGACCAGCTACCA
CAACAAAATAAGGTAGCTTAACTAGAGCAGAATATCACTTATAAATAAGCTTTTAGTTGTTCAAACAAGTGGGACCATCTCT
CCAAGAAGAAATCATTCAAATGACTTTAAAGCTAAGGTAGCGCTTGCTGCGATTAAAGCAGAAAAAACACTTGCTGAATTGAGTGCTGAATTTGATGTTC
ATCAAAACCAAATTATCGACTGGAAAAATCAATTGCTTTCAGCTTCCTCGCAAGCCTTCGATCAATCAAAAGCTCCAGCAGAACCGCCCATTGATCTAAA
AAAACTACATGCAAAAATCGGTGAACAGGCATTAGAAATTGATTTTTTAGAAGGTGTGTTGAAGAAACTGGGCCGCTTCAACCACAAAAGTTAATCGACG
ACTCACTTCAGATTTCAGTATCTAAGCAAGCTAAGCTACTGAAAGTCTCCCGTGGTTGTTATTACTATCGCCCAAAACCTGTGAGTGCATCAGATCTGAA
GCTGATGCGCTGTATTGATGAATTACATATGCAATACCCTTTTGCAGGTAGCCGTATGATGCGTGATTTATTGAACCGTCAAGGACATCATATAGGACGA
CGTCATACACGTACTTTAATGAAGAAAATGGGCATTAATGCGTTATATCGTAAACCAAATCTAAGTCAGGCCAATCAAGCTCACCGTAAATATCCATATC
TGCTCAAAGGATTGAATATTCAACGGAGTAATCAAGTGTGGGCAACGGATATAACGTATATCCCTATGGCAAAAGGCTTTGTCTACTTATGTGCTGTGAT
TGATTGGCACAGCCGTAAGGTGCTTGCCCATAGCGTATCGATTAGTATGGAGGTTGCATTTTGTATAGAAACATTAAATGAAGCTATTGAAAAATATGGC
CGACCTGAAATCTTTAATACAGACCAAGGCAGTCAATTTACCAGTGATGCATTTATTGAAGTGTTAAAATCAAATGACATCCAAATCAGCATGGATGGTA
AAGGTCGTTGGGTTGATAATGTGATGATTGAACGATTATGGCGGAGCGTTAAATACGAGGAGGTTTATCTCAAAGCCTACAGCAATGTTTTTGATGCGAA
AAAGCAATTAAATGCCTATTTTGAATTTTATAATTTGAAACGACCTCATTCGAGTCTGGACAAAATGACTCCAGATGAGTTTTACTATGACCAGCTACCA
CAACAAAATAAGGTAGCTTAACTAGAGCAGAATATCACTTATAAATAAGCTTTTAGTTGTTCAAACAAGTGGGACCATCTCT
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 318 bp | 105 aa | 77 | 394 | + | No |
Description : First part of the transposase
ORF sequence :
MEYFMARRPRRNHSNDFKAKVALAAIKAEKTLAELSAEFDVHQNQIIDWKNQLLSASSQAFDQSKAPAEPPIDLKKLHAKIGEQALEIDFLEGVLKKLGR
FNHKS
FNHKS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 879 bp | 292 aa | 343 | 1221 | + | No |
Description : Second part of the transposase
ORF sequence :
FFRRCVEETGPLQPQKLIDDSLQISVSKQAKLLKVSRGCYYYRPKPVSASDLKLMRCIDELHMQYPFAGSRMMRDLLNRQGHHIGRRHTRTLMKKMGINA
LYRKPNLSQANQAHRKYPYLLKGLNIQRSNQVWATDITYIPMAKGFVYLCAVIDWHSRKVLAHSVSISMEVAFCIETLNEAIEKYGRPEIFNTDQGSQFT
SDAFIEVLKSNDIQISMDGKGRWVDNVMIERLWRSVKYEEVYLKAYSNVFDAKKQLNAYFEFYNLKRPHSSLDKMTPDEFYYDQLPQQNKVA
LYRKPNLSQANQAHRKYPYLLKGLNIQRSNQVWATDITYIPMAKGFVYLCAVIDWHSRKVLAHSVSISMEVAFCIETLNEAIEKYGRPEIFNTDQGSQFT
SDAFIEVLKSNDIQISMDGKGRWVDNVMIERLWRSVKYEEVYLKAYSNVFDAKKQLNAYFEFYNLKRPHSSLDKMTPDEFYYDQLPQQNKVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1145 bp | 381 aa | 77 | 1221 | + | Yes |
Chemistry : DDE
ORF sequence :
MEYFMARRPRRNHSNDFKAKVALAAIKAEKTLAELSAEFDVHQNQIIDWKNQLLSASSQAFDQSKAPAEPPIDLKKLHAKIGEQALEIDFFRRCVEETGP
LQPQKLIDDSLQISVSKQAKLLKVSRGCYYYRPKPVSASDLKLMRCIDELHMQYPFAGSRMMRDLLNRQGHHIGRRHTRTLMKKMGINALYRKPNLSQAN
QAHRKYPYLLKGLNIQRSNQVWATDITYIPMAKGFVYLCAVIDWHSRKVLAHSVSISMEVAFCIETLNEAIEKYGRPEIFNTDQGSQFTSDAFIEVLKSN
DIQISMDGKGRWVDNVMIERLWRSVKYEEVYLKAYSNVFDAKKQLNAYFEFYNLKRPHSSLDKMTPDEFYYDQLPQQNKVA
LQPQKLIDDSLQISVSKQAKLLKVSRGCYYYRPKPVSASDLKLMRCIDELHMQYPFAGSRMMRDLLNRQGHHIGRRHTRTLMKKMGINALYRKPNLSQAN
QAHRKYPYLLKGLNIQRSNQVWATDITYIPMAKGFVYLCAVIDWHSRKVLAHSVSISMEVAFCIETLNEAIEKYGRPEIFNTDQGSQFTSDAFIEVLKSN
DIQISMDGKGRWVDNVMIERLWRSVKYEEVYLKAYSNVFDAKKQLNAYFEFYNLKRPHSSLDKMTPDEFYYDQLPQQNKVA
Blast result :
Comments
ISAlw27 is 97% aa (transposase) similar to ISAba14.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
Acinetobacter lwoffii EK30A plasmid pALWEK1.1 carries 2 identical copies of ISAlw27.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
Acinetobacter lwoffii EK30A plasmid pALWEK1.1 carries 2 identical copies of ISAlw27.
References