ISAlw26
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP019143.1 | ND | Acinetobacter lwoffii | Acinetobacter lwoffii Acinetobacter lwoffii ZS207 |
DNA section
IS Length : 1238 bp
Ends
IR Length : 39
IRL : TGATCCTAGACAAAATAATGGACAGCACGTCCCTCTAAAGATAACGTAAC
IRR : TGATCCTAGACAAAATAATGGACAACCAGCCCCTCTAAACTTTTAATATA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TGATCCTAGACAAAATAATGGACAGCACGTCCCTCTAAAGATAACGTAACTTTAATCTTTGGAGATTCAAGAAATGGCTAAACGTTTTAGTCCCGAATTT
AAACAGCAAGCGATTGATTATGCACTTTCAAATTCACACGAGCCTATAGCTGCAATCGCCCAGAAATTAGGTGTGGGTTATTCAACATTAGATAAATGGA
TTCGTGAAACCAATCCGGCAGGTTCAAGCAAACGTCAACTTTCACCAGAACAACAGCGGATCGTGGAATTAGAGAAAGAAGTCAAACAGCTCAAGGAAGC
CAATGACATCTTAAAAAAAGCGCATGTGTACTTTCTAACAGATCATGCCAAGAAAAGTACACGGTAATTCAAGATCTAGATATGAATGAAGTCACCGTAT
CTTCTGCCTGTAAATACCTAGGTGTCAGCACTTCAGGCTATTATGCCTGGCGAAAACGTCAAGCCAATACGGCACAGAAATATAATGACTTAAAAGCCGT
ATATTGGCAGCATCATGCACGATTGGGTGCACCTTCATTAGTACATGACATGCATGATTTAGGTTACAACATGAGCGAACGTACTATTGGAAGGATGCTA
AAAAAGCTTGGTTTACGTAGCAGGATTGCGCGTAAATACAAGCATACGACTGATTCAACCCATCGTTTGCCTACAGCACCCAACTTGTTGGATCGCCAAT
TTACAGTTACTCAGCCTAATAAAGTCTGGACAACAGACATTACCTATATCCGCACTAAAGAAGGTTGGTTGTATTTATGTGTAATGCTAGATTTATTTAG
CCGTCGTATCGTGGGGTGGCAAACCAGCCATCGAATAGACCGCCAGTTGGTGTGTGATACGTTTAACTATGCAATGGCTCGTCAGGGTTATCCAACTGGT
GTTATGGTTCATTCGGACCAAGGCTCACAGTACTGTAGTCGTGATTTTAGAGCGCTGTTACTGAAAAATGATTGTACTCAGAGTATGTCTAGACGTGGAA
ACTGTTGGGATAATGCAGTGACTGAAAGCTTCTTTCATACATTAAAAGGTCATGTGGTACATGGCAGTGTGTTTTCGACTCGAAAAGAAGCGAATGCTGT
CTTGTTTGACTATATTGAAATTTATTACAATCGAGTCAGAAGACATTCTGCAAACGGCTGGTTAAGTCCAGAAGCCTTTGAACAGAAATATATTAAAAGT
TTAGAGGGGCTGGTTGTCCATTATTTTGTCTAGGATCA
AAACAGCAAGCGATTGATTATGCACTTTCAAATTCACACGAGCCTATAGCTGCAATCGCCCAGAAATTAGGTGTGGGTTATTCAACATTAGATAAATGGA
TTCGTGAAACCAATCCGGCAGGTTCAAGCAAACGTCAACTTTCACCAGAACAACAGCGGATCGTGGAATTAGAGAAAGAAGTCAAACAGCTCAAGGAAGC
CAATGACATCTTAAAAAAAGCGCATGTGTACTTTCTAACAGATCATGCCAAGAAAAGTACACGGTAATTCAAGATCTAGATATGAATGAAGTCACCGTAT
CTTCTGCCTGTAAATACCTAGGTGTCAGCACTTCAGGCTATTATGCCTGGCGAAAACGTCAAGCCAATACGGCACAGAAATATAATGACTTAAAAGCCGT
ATATTGGCAGCATCATGCACGATTGGGTGCACCTTCATTAGTACATGACATGCATGATTTAGGTTACAACATGAGCGAACGTACTATTGGAAGGATGCTA
AAAAAGCTTGGTTTACGTAGCAGGATTGCGCGTAAATACAAGCATACGACTGATTCAACCCATCGTTTGCCTACAGCACCCAACTTGTTGGATCGCCAAT
TTACAGTTACTCAGCCTAATAAAGTCTGGACAACAGACATTACCTATATCCGCACTAAAGAAGGTTGGTTGTATTTATGTGTAATGCTAGATTTATTTAG
CCGTCGTATCGTGGGGTGGCAAACCAGCCATCGAATAGACCGCCAGTTGGTGTGTGATACGTTTAACTATGCAATGGCTCGTCAGGGTTATCCAACTGGT
GTTATGGTTCATTCGGACCAAGGCTCACAGTACTGTAGTCGTGATTTTAGAGCGCTGTTACTGAAAAATGATTGTACTCAGAGTATGTCTAGACGTGGAA
ACTGTTGGGATAATGCAGTGACTGAAAGCTTCTTTCATACATTAAAAGGTCATGTGGTACATGGCAGTGTGTTTTCGACTCGAAAAGAAGCGAATGCTGT
CTTGTTTGACTATATTGAAATTTATTACAATCGAGTCAGAAGACATTCTGCAAACGGCTGGTTAAGTCCAGAAGCCTTTGAACAGAAATATATTAAAAGT
TTAGAGGGGCTGGTTGTCCATTATTTTGTCTAGGATCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 297 bp | 97 aa | 74 | 370 | + | No |
Description : First part of the transposase
ORF sequence :
MAKRFSPEFKQQAIDYALSNSHEPIAAIAQKLGVGYSTLDKWIRETNPAGSSKRQLSPEQQRIVELEKEVKQLKEANDILKKAHVYFLTDHAKKSTR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 930 bp | 308 aa | 307 | 1236 | + | No |
Description : Second part of the transposase
ORF sequence :
HLKKSACVLSNRSCQEKYTVIQDLDMNEVTVSSACKYLGVSTSGYYAWRKRQANTAQKYNDLKAVYWQHHARLGAPSLVHDMHDLGYNMSERTIGRMLKK
LGLRSRIARKYKHTTDSTHRLPTAPNLLDRQFTVTQPNKVWTTDITYIRTKEGWLYLCVMLDLFSRRIVGWQTSHRIDRQLVCDTFNYAMARQGYPTGVM
VHSDQGSQYCSRDFRALLLKNDCTQSMSRRGNCWDNAVTESFFHTLKGHVVHGSVFSTRKEANAVLFDYIEIYYNRVRRHSANGWLSPEAFEQKYIKSLE
GLVVHYFV
LGLRSRIARKYKHTTDSTHRLPTAPNLLDRQFTVTQPNKVWTTDITYIRTKEGWLYLCVMLDLFSRRIVGWQTSHRIDRQLVCDTFNYAMARQGYPTGVM
VHSDQGSQYCSRDFRALLLKNDCTQSMSRRGNCWDNAVTESFFHTLKGHVVHGSVFSTRKEANAVLFDYIEIYYNRVRRHSANGWLSPEAFEQKYIKSLE
GLVVHYFV
Blast result :Comments : Second part of transposase.ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1163 bp | 386 aa | 74 | 1236 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKRFSPEFKQQAIDYALSNSHEPIAAIAQKLGVGYSTLDKWIRETNPAGSSKRQLSPEQQRIVELEKEVKQLKEANDILKKSACVLSNRSCQEKYTVIQ
DLDMNEVTVSSACKYLGVSTSGYYAWRKRQANTAQKYNDLKAVYWQHHARLGAPSLVHDMHDLGYNMSERTIGRMLKKLGLRSRIARKYKHTTDSTHRLP
TAPNLLDRQFTVTQPNKVWTTDITYIRTKEGWLYLCVMLDLFSRRIVGWQTSHRIDRQLVCDTFNYAMARQGYPTGVMVHSDQGSQYCSRDFRALLLKND
CTQSMSRRGNCWDNAVTESFFHTLKGHVVHGSVFSTRKEANAVLFDYIEIYYNRVRRHSANGWLSPEAFEQKYIKSLEGLVVHYFV
DLDMNEVTVSSACKYLGVSTSGYYAWRKRQANTAQKYNDLKAVYWQHHARLGAPSLVHDMHDLGYNMSERTIGRMLKKLGLRSRIARKYKHTTDSTHRLP
TAPNLLDRQFTVTQPNKVWTTDITYIRTKEGWLYLCVMLDLFSRRIVGWQTSHRIDRQLVCDTFNYAMARQGYPTGVMVHSDQGSQYCSRDFRALLLKND
CTQSMSRRGNCWDNAVTESFFHTLKGHVVHGSVFSTRKEANAVLFDYIEIYYNRVRRHSANGWLSPEAFEQKYIKSLEGLVVHYFV
Blast result :
Comments
ISAlw26 is 97% aa (transposase) similar to ISAcsp3.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISAlw26 is 91% and 90% similar to ISAcsp3 and IS1236, respectively. Its closest relatives (99% similar) are present in Acinetobacter sp. WCHAc010005 (CP032134.1) and A. haemolyticus TJR01 (CP038009.1) chromosome in two and four copies, respectively.
copies and
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISAlw26 is 91% and 90% similar to ISAcsp3 and IS1236, respectively. Its closest relatives (99% similar) are present in Acinetobacter sp. WCHAc010005 (CP032134.1) and A. haemolyticus TJR01 (CP038009.1) chromosome in two and four copies, respectively.
copies and
References