ISAlw28
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP019144 | ND | Acinetobacter lwoffii | Acinetobacter lwoffii Acinetobacter lwoffii ZS207 plasmid pmZS |
DNA section
IS Length : 1184 bp
Ends
IR Length : 36/37
IRL : GTAATCCCTCCTAAAAACAATAGGACCTAAAAGTAGAATTTTCTCTTAAA
IRR : GTAATCCTCCCATAAATAACTGAAGTTAAAAGTAGAGGTTAAGCAGCCAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GTAATCCCTCCTAAAAACAATAGGACCTAAAAGTAGAATTTTCTCTTAAAGTATAGGCAGTAGATTCTGCATGAAAACATCTAAATTTACTGACAGTCAG
ATCATGTCCATTTTGAAACAGGGGGAGTCTGGCACACCTGTAGCCGCCCTTTGTCGTGAACACGGTATGAGTAATGCGACCTTCTATAAATGGCGTGCCA
AATATGGCGGTATGGATACATCATTAATGGCAAAACTCAAAGAACTGGAAGCAGAAAATACCAGACTTAAAAAGATGTATGCTGAGGAACGCTTAAAGGC
AGAGATCATTCAGGAAGCGATGGCAAAAAAGTGGTGAGGCCATCTTGCCGACGTAAGATGCCACAACAGGCAGTTGCCCACTATGCGATTAGCATTCGTT
TGGCTTGCCGAGCATTTAGTATCAGTGAAACCTGCTACCGTTATCAATCCAAGTTAAACGATGACAATACATTAATTGCAGAACAGCTCATTGAGCTCAC
TGAAGAAAATACAGATTGGGGTTTTGGTCTGTGTTTCTCTTATCTACGTCATGTTGAAAACCATGTTTGGAATCATAAACGTGTTTATCGCATTTACTGT
GAGTCAGCACTGAATCTGCGCATTAAACCGAGAAGAAGACTAAAACGGCATGCACCAGAACCACTGAAAGAGCCGATCCGGGCAAATCAGGTCTGGTCAC
TGGATTTTATGCATGACCAGCTGATCGATAGTCGTAAGTTCCGATTGTTGAATGTGATTGATGATTACCGTCGAGAAGGTTTGACTATTGAAGCAGAATT
CTCATTACCCACGATCAGGGTTATTCACACATTGAATCAGTTATTGGAATGGCGAGAAAAACCGTTAGTTATTCGGTGTGACAATGGCCCGGAGTTTATC
AGTCACGAATTTGTACGCTGGGCAACTGAACAAGGTATTCGTATTGAATATATTCAACCAGGTAAACCACAGCAGAATGCGTATATTGAACGCTATAATC
GGACTATACGTTATAGTTGGCTGAGCAAACATTTATTTGATACTTTGGATGAAGTACAAGATTATGCAACAAACTGGCTGTGGCATTACAACCATGAAAG
ACCACACCAAGCAAACAAAGGAAGGCCACCTCTAATGGCTGCTTAACCTCTACTTTTAACTTCAGTTATTTATGGGAGGATTAC
ATCATGTCCATTTTGAAACAGGGGGAGTCTGGCACACCTGTAGCCGCCCTTTGTCGTGAACACGGTATGAGTAATGCGACCTTCTATAAATGGCGTGCCA
AATATGGCGGTATGGATACATCATTAATGGCAAAACTCAAAGAACTGGAAGCAGAAAATACCAGACTTAAAAAGATGTATGCTGAGGAACGCTTAAAGGC
AGAGATCATTCAGGAAGCGATGGCAAAAAAGTGGTGAGGCCATCTTGCCGACGTAAGATGCCACAACAGGCAGTTGCCCACTATGCGATTAGCATTCGTT
TGGCTTGCCGAGCATTTAGTATCAGTGAAACCTGCTACCGTTATCAATCCAAGTTAAACGATGACAATACATTAATTGCAGAACAGCTCATTGAGCTCAC
TGAAGAAAATACAGATTGGGGTTTTGGTCTGTGTTTCTCTTATCTACGTCATGTTGAAAACCATGTTTGGAATCATAAACGTGTTTATCGCATTTACTGT
GAGTCAGCACTGAATCTGCGCATTAAACCGAGAAGAAGACTAAAACGGCATGCACCAGAACCACTGAAAGAGCCGATCCGGGCAAATCAGGTCTGGTCAC
TGGATTTTATGCATGACCAGCTGATCGATAGTCGTAAGTTCCGATTGTTGAATGTGATTGATGATTACCGTCGAGAAGGTTTGACTATTGAAGCAGAATT
CTCATTACCCACGATCAGGGTTATTCACACATTGAATCAGTTATTGGAATGGCGAGAAAAACCGTTAGTTATTCGGTGTGACAATGGCCCGGAGTTTATC
AGTCACGAATTTGTACGCTGGGCAACTGAACAAGGTATTCGTATTGAATATATTCAACCAGGTAAACCACAGCAGAATGCGTATATTGAACGCTATAATC
GGACTATACGTTATAGTTGGCTGAGCAAACATTTATTTGATACTTTGGATGAAGTACAAGATTATGCAACAAACTGGCTGTGGCATTACAACCATGAAAG
ACCACACCAAGCAAACAAAGGAAGGCCACCTCTAATGGCTGCTTAACCTCTACTTTTAACTTCAGTTATTTATGGGAGGATTAC
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 270 bp | 88 aa | 71 | 340 | + | No |
Description : First part of the transposase
ORF sequence :
MKTSKFTDSQIMSILKQGESGTPVAALCREHGMSNATFYKWRAKYGGMDTSLMAKLKELEAENTRLKKMYAEERLKAEIIQEAMAKKW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 864 bp | 286 aa | 286 | 1149 | + | No |
Description : Second part of the transposase
ORF sequence :
GTLKGRDHSGSDGKKVVRPSCRRKMPQQAVAHYAISIRLACRAFSISETCYRYQSKLNDDNTLIAEQLIELTEENTDWGFGLCFSYLRHVENHVWNHKRV
YRIYCESALNLRIKPRRRLKRHAPEPLKEPIRANQVWSLDFMHDQLIDSRKFRLLNVIDDYRREGLTIEAEFSLPTIRVIHTLNQLLEWREKPLVIRCDN
GPEFISHEFVRWATEQGIRIEYIQPGKPQQNAYIERYNRTIRYSWLSKHLFDTLDEVQDYATNWLWHYNHERPHQANKGRPPLMAA
YRIYCESALNLRIKPRRRLKRHAPEPLKEPIRANQVWSLDFMHDQLIDSRKFRLLNVIDDYRREGLTIEAEFSLPTIRVIHTLNQLLEWREKPLVIRCDN
GPEFISHEFVRWATEQGIRIEYIQPGKPQQNAYIERYNRTIRYSWLSKHLFDTLDEVQDYATNWLWHYNHERPHQANKGRPPLMAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1079 bp | 358 aa | 71 | 1149 | + | Yes |
Chemistry : DDE
ORF sequence :
MKTSKFTDSQIMSILKQGESGTPVAALCREHGMSNATFYKWRAKYGGMDTSLMAKLKELEAENTRLKKMYAEERLKAEIIQEAMAKKVVRPSCRRKMPQQ
AVAHYAISIRLACRAFSISETCYRYQSKLNDDNTLIAEQLIELTEENTDWGFGLCFSYLRHVENHVWNHKRVYRIYCESALNLRIKPRRRLKRHAPEPLK
EPIRANQVWSLDFMHDQLIDSRKFRLLNVIDDYRREGLTIEAEFSLPTIRVIHTLNQLLEWREKPLVIRCDNGPEFISHEFVRWATEQGIRIEYIQPGKP
QQNAYIERYNRTIRYSWLSKHLFDTLDEVQDYATNWLWHYNHERPHQANKGRPPLMAA
AVAHYAISIRLACRAFSISETCYRYQSKLNDDNTLIAEQLIELTEENTDWGFGLCFSYLRHVENHVWNHKRVYRIYCESALNLRIKPRRRLKRHAPEPLK
EPIRANQVWSLDFMHDQLIDSRKFRLLNVIDDYRREGLTIEAEFSLPTIRVIHTLNQLLEWREKPLVIRCDNGPEFISHEFVRWATEQGIRIEYIQPGKP
QQNAYIERYNRTIRYSWLSKHLFDTLDEVQDYATNWLWHYNHERPHQANKGRPPLMAA
Blast result :
Comments
ISAlw28 is 97% aa (transposase) similar to ISAca1.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISAlw28 is 93% similar to ISAca1. The closest relatives (99 and 98% similar) occur in A. lwoffii NCTC5866 (LR215976.1) and A. johnsonii IC001 (CP022298.1). Neither of these copies are flanked by DRs.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISAlw28 is 93% similar to ISAca1. The closest relatives (99 and 98% similar) occur in A. lwoffii NCTC5866 (LR215976.1) and A. johnsonii IC001 (CP022298.1). Neither of these copies are flanked by DRs.
References