ISAlw23
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Acinetobacter lwoffii | Acinetobacter lwoffii Acinetobacter lwoffii M2a |
DNA section
IS Length : 1308 bp
Ends
IR Length : 25
IRL : TGAACCGTACCGGGTTTGTCGGAGAGTCAATATTCTGAGAGACTATCCCG
IRR : TGAACCGTACCGGGTTTGTCGGAGACTTTTTATTTAAGTTAGGCCACCTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| 0 |
DNA sequence
TGAACCGTACCGGGTTTGTCGGAGAGTCAATATTCTGAGAGACTATCCCGATGACAAAATTAAAATATACCCCTGAAATCAGAGAAAGAGCGGTTCAATT
ATTGATTGAATCTGAAAAAGATTATCCATCGAATTGGGCTGCGATCACCGCTATTGCTCCCAAGATAGGTTGTACTCCTGAAACACTCCGTGTTTGGTAT
CAAAAATACTTAGATCAACAAAATCCTATTAAAGTACAACAGATATCTGATCAAGAAAAAATGAAGCAAATGGAACGTGAAATTAAAGAATTAAAGCGTG
CCAATGAAATTCTACGTAAAGCAGCCGCTTTTTTCGCCCAGGCGGAGCTCGACCGCCCACACAAATAATGGTGGATTTTATCCATAACAATAAAGACTTA
TATGGTGTTGATGCGATTTGTAGGATTTTACCGATCGCAGCTTCAACCTATTACCGGACTCTAGATCTCTGCGAAAATCCAGAACATCGAGCAAAGCGAG
ATTTACATGATTTACATCATGCAGAGCAAATCAAACGTATTTGGAAAGAAAGTTCAGGTCGATATGGTGTACGTAAAGTTTGGCAACAATTGAAACGTGA
GGGTCATGTTATTGCACGTTGTACAGTTGCTCGATTGATGCAAAAGCTAGATATACAAGGTGTTTGGCGTGGTAAGAACAAACAAACTACCCGCAGCCGA
GATGATCAAAAACGGGCAGGTGATTTAGTGAAACGGAATTTTAGTGCTGATCGACCTGACCAATTATGGGTCAGTGACTTTACGTATATTCAAACCAATT
CTGGCTGGGTCTACACCGCATTTATTATTGATGTGTTCTCACGAGCAATTGTTGGATGGAAAGTATCTACACGGATGAATACAGATATGGTGCTCGATGC
ATTGGAGCAAGCATTGCACGATCGAGGCATGCCAAAGAATGTAATTCATCATTCAGATAGAGGTGTTCAATATCTTTCTATTCGCTATACCAATCGTTTA
GAAGCAGCAAATTTACGAGCATCAGTCGGTACAACGGGTGATTCATACGATAATGCTTTGGCTGAAACGGTGAATGGCTTATACAAAACAGAGGTGATTG
AATATTTAAAAGCAGATTGGCAAGGTTTAGCAGATGTACAACTTGCGACATTAAATTGGGTAGATTGGTTCAACAAAAAGCGTGTACACGGTGCACTGGG
TTATGTATCGCCTTTTGAGTTTGAAGCAATGTACTATGATAAGATTAATCCGTTAGGTCAGGTGGCCTAACTTAAATAAAAAGTCTCCGACAAACCCGGT
ACGGTTCA
ATTGATTGAATCTGAAAAAGATTATCCATCGAATTGGGCTGCGATCACCGCTATTGCTCCCAAGATAGGTTGTACTCCTGAAACACTCCGTGTTTGGTAT
CAAAAATACTTAGATCAACAAAATCCTATTAAAGTACAACAGATATCTGATCAAGAAAAAATGAAGCAAATGGAACGTGAAATTAAAGAATTAAAGCGTG
CCAATGAAATTCTACGTAAAGCAGCCGCTTTTTTCGCCCAGGCGGAGCTCGACCGCCCACACAAATAATGGTGGATTTTATCCATAACAATAAAGACTTA
TATGGTGTTGATGCGATTTGTAGGATTTTACCGATCGCAGCTTCAACCTATTACCGGACTCTAGATCTCTGCGAAAATCCAGAACATCGAGCAAAGCGAG
ATTTACATGATTTACATCATGCAGAGCAAATCAAACGTATTTGGAAAGAAAGTTCAGGTCGATATGGTGTACGTAAAGTTTGGCAACAATTGAAACGTGA
GGGTCATGTTATTGCACGTTGTACAGTTGCTCGATTGATGCAAAAGCTAGATATACAAGGTGTTTGGCGTGGTAAGAACAAACAAACTACCCGCAGCCGA
GATGATCAAAAACGGGCAGGTGATTTAGTGAAACGGAATTTTAGTGCTGATCGACCTGACCAATTATGGGTCAGTGACTTTACGTATATTCAAACCAATT
CTGGCTGGGTCTACACCGCATTTATTATTGATGTGTTCTCACGAGCAATTGTTGGATGGAAAGTATCTACACGGATGAATACAGATATGGTGCTCGATGC
ATTGGAGCAAGCATTGCACGATCGAGGCATGCCAAAGAATGTAATTCATCATTCAGATAGAGGTGTTCAATATCTTTCTATTCGCTATACCAATCGTTTA
GAAGCAGCAAATTTACGAGCATCAGTCGGTACAACGGGTGATTCATACGATAATGCTTTGGCTGAAACGGTGAATGGCTTATACAAAACAGAGGTGATTG
AATATTTAAAAGCAGATTGGCAAGGTTTAGCAGATGTACAACTTGCGACATTAAATTGGGTAGATTGGTTCAACAAAAAGCGTGTACACGGTGCACTGGG
TTATGTATCGCCTTTTGAGTTTGAAGCAATGTACTATGATAAGATTAATCCGTTAGGTCAGGTGGCCTAACTTAAATAAAAAGTCTCCGACAAACCCGGT
ACGGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 318 bp | 105 aa | 51 | 368 | + | No |
Description : First part of the transposase
ORF sequence :
MTKLKYTPEIRERAVQLLIESEKDYPSNWAAITAIAPKIGCTPETLRVWYQKYLDQQNPIKVQQISDQEKMKQMEREIKELKRANEILRKAAAFFAQAEL
DRPHK
DRPHK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 951 bp | 316 aa | 320 | 1270 | + | No |
Description : Second part of the transposase
ORF sequence :
SSRFFRPGGARPPTQIMVDFIHNNKDLYGVDAICRILPIAASTYYRTLDLCENPEHRAKRDLHDLHHAEQIKRIWKESSGRYGVRKVWQQLKREGHVIAR
CTVARLMQKLDIQGVWRGKNKQTTRSRDDQKRAGDLVKRNFSADRPDQLWVSDFTYIQTNSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALH
DRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHGALGYVSPFE
FEAMYYDKINPLGQVA
CTVARLMQKLDIQGVWRGKNKQTTRSRDDQKRAGDLVKRNFSADRPDQLWVSDFTYIQTNSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALH
DRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHGALGYVSPFE
FEAMYYDKINPLGQVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1220 bp | 406 aa | 51 | 1270 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKLKYTPEIRERAVQLLIESEKDYPSNWAAITAIAPKIGCTPETLRVWYQKYLDQQNPIKVQQISDQEKMKQMEREIKELKRANEILRKAAAFFRPGGA
RPPTQIMVDFIHNNKDLYGVDAICRILPIAASTYYRTLDLCENPEHRAKRDLHDLHHAEQIKRIWKESSGRYGVRKVWQQLKREGHVIARCTVARLMQKL
DIQGVWRGKNKQTTRSRDDQKRAGDLVKRNFSADRPDQLWVSDFTYIQTNSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALHDRGMPKNVIH
HSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHGALGYVSPFEFEAMYYDKIN
PLGQVA
RPPTQIMVDFIHNNKDLYGVDAICRILPIAASTYYRTLDLCENPEHRAKRDLHDLHHAEQIKRIWKESSGRYGVRKVWQQLKREGHVIARCTVARLMQKL
DIQGVWRGKNKQTTRSRDDQKRAGDLVKRNFSADRPDQLWVSDFTYIQTNSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALHDRGMPKNVIH
HSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHGALGYVSPFEFEAMYYDKIN
PLGQVA
Blast result :Comments : The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
Comments
ISAlw23 is 98% aa (transposase) similar to ISAba2.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References