ISAlw5
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Acinetobacter lwoffii | Acinetobacter lwoffii Acinetobacter lwoffii M2a |
DNA section
IS Length : 1309 bp
Ends
IR Length : 25
IRL : TGAACCGTACCGGGTTTGTCGGAGACTCAATATTCTGAGAGACTATTCCG
IRR : TGAACCGTACCGGGTTTGTCGGAGACTTTTTTATTTAAGTTAAGCCACCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| gaaatcaggtcgtcctcata | ctt | tagatatagaggatcagttg | 3 |
DNA sequence
TGAACCGTACCGGGTTTGTCGGAGACTCAATATTCTGAGAGACTATTCCGATGAAAAAACCAACCTATACCCCCGAAATTAGAGAAAGAGCGGTTCAATT
ACTAATTGAATCTGAAAAAGATTATCCTTCTACTTGGGCTGCAATCACAGCAATTGCGCCTAAGATTGGTTGTACTCCTGAAACACTTCGTGCCTGGCAT
CAAAAGCATTTAGATCAGCAAAATCCTATTAAAGTACAACAGATATCTGATCAAGAAAAAATGAAGCAAATGGAACGTGAAATTAAAGAATTAAAGCGTG
CCAATGAAATTCTACGTAAAGCAGCCGCTTTTTTCGCCTAGGCGGAGCTCGACCGCCCACACAAATAATGGTGGATTTCATCCATAACAATAAAGACTTA
TATGGTGTTGATGCGATTTGTAGGATTTTACCGATCGCAGCTTCAACCTATTACCGAACTTTAGATCTCGCTGACAATCCAGAACATCGAGCGAAACGAG
ATCTACATGATGAGCATCATGCTGAACAAATTAAACGAATTTGGAAGGAAAGTTTAGGTCGATATGGTGTACGTAAAGTTTGGCAAAAATTGAAACGTGA
AGGCTATGTTATTGGACGTTGTACAGTTGCTCGATTGATGCAGAAGCTAGATATACAAGGTGTTTGGCGTGGTAAGAACAAACAAACTACCCGCAACCGA
GATGATCAAAAACGAGCAGATGATTTAGTGAAACGTAATTTTAGTGCTGATCGACCTGACCAATTATGGGTCAGTGACTTTACGTATATTCAAACTCATT
CAGGCTGGGTCTATACCGCCTTTATTATTGATGTGTTCTCACGAGCAATTGTTGGATGGAAAGTATCTACACGGATGAATACAGATATGGTGCTCGATGC
ACTTGAGCAAGCATTGCACGATCGAGGCATGCCAAAGAATGTGATTCATCATTCCGACAGGGGTGTGCAATATCTTTCGATTCGCTATACCAATCGTTTA
GAAGCAGCAAATTTACGAGCATCAGTCGGTACAACTGGTGATTCATACGATAATGCTTTGGCTGAAACGGTGAATGGCTTATACAAAACAGAGGTGATTG
AATATTTAAAAGCAGATTGGCAAGGTTTAGCAGATGTACAACTTGCGACACTAAACTGGGTAGATTGGTTCAATAAAAAGCGTGTACACAGTGCACTGGG
TTATGTGTCGCCTTTTGAGTTTGAAGCAATGTACTATGATAAGATTAACCCGTTAGGTCAGGTGGCTTAACTTAAATAAAAAAGTCTCCGACAAACCCGG
TACGGTTCA
ACTAATTGAATCTGAAAAAGATTATCCTTCTACTTGGGCTGCAATCACAGCAATTGCGCCTAAGATTGGTTGTACTCCTGAAACACTTCGTGCCTGGCAT
CAAAAGCATTTAGATCAGCAAAATCCTATTAAAGTACAACAGATATCTGATCAAGAAAAAATGAAGCAAATGGAACGTGAAATTAAAGAATTAAAGCGTG
CCAATGAAATTCTACGTAAAGCAGCCGCTTTTTTCGCCTAGGCGGAGCTCGACCGCCCACACAAATAATGGTGGATTTCATCCATAACAATAAAGACTTA
TATGGTGTTGATGCGATTTGTAGGATTTTACCGATCGCAGCTTCAACCTATTACCGAACTTTAGATCTCGCTGACAATCCAGAACATCGAGCGAAACGAG
ATCTACATGATGAGCATCATGCTGAACAAATTAAACGAATTTGGAAGGAAAGTTTAGGTCGATATGGTGTACGTAAAGTTTGGCAAAAATTGAAACGTGA
AGGCTATGTTATTGGACGTTGTACAGTTGCTCGATTGATGCAGAAGCTAGATATACAAGGTGTTTGGCGTGGTAAGAACAAACAAACTACCCGCAACCGA
GATGATCAAAAACGAGCAGATGATTTAGTGAAACGTAATTTTAGTGCTGATCGACCTGACCAATTATGGGTCAGTGACTTTACGTATATTCAAACTCATT
CAGGCTGGGTCTATACCGCCTTTATTATTGATGTGTTCTCACGAGCAATTGTTGGATGGAAAGTATCTACACGGATGAATACAGATATGGTGCTCGATGC
ACTTGAGCAAGCATTGCACGATCGAGGCATGCCAAAGAATGTGATTCATCATTCCGACAGGGGTGTGCAATATCTTTCGATTCGCTATACCAATCGTTTA
GAAGCAGCAAATTTACGAGCATCAGTCGGTACAACTGGTGATTCATACGATAATGCTTTGGCTGAAACGGTGAATGGCTTATACAAAACAGAGGTGATTG
AATATTTAAAAGCAGATTGGCAAGGTTTAGCAGATGTACAACTTGCGACACTAAACTGGGTAGATTGGTTCAATAAAAAGCGTGTACACAGTGCACTGGG
TTATGTGTCGCCTTTTGAGTTTGAAGCAATGTACTATGATAAGATTAACCCGTTAGGTCAGGTGGCTTAACTTAAATAAAAAAGTCTCCGACAAACCCGG
TACGGTTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 318 bp | 96 aa | 51 | 368 | + | No |
Description : First part of the transposase
ORF sequence :
MKKPTYTPEIRERAVQLLIESEKDYPSTWAAITAIAPKIGCTPETLRAWHQKHLDQQNPIKVQQISDQEKMKQMEREIKELKRANEILRKAAAFFA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 951 bp | 316 aa | 320 | 1270 | + | No |
Description : Second part of the transposase
ORF sequence :
SSRFFRLGGARPPTQIMVDFIHNNKDLYGVDAICRILPIAASTYYRTLDLADNPEHRAKRDLHDEHHAEQIKRIWKESLGRYGVRKVWQKLKREGYVIGR
CTVARLMQKLDIQGVWRGKNKQTTRNRDDQKRADDLVKRNFSADRPDQLWVSDFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALH
DRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHSALGYVSPFE
FEAMYYDKINPLGQVA
CTVARLMQKLDIQGVWRGKNKQTTRNRDDQKRADDLVKRNFSADRPDQLWVSDFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALH
DRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHSALGYVSPFE
FEAMYYDKINPLGQVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1220 bp | 406 aa | 51 | 1270 | - | No |
ORF sequence :
MKKPTYTPEIRERAVQLLIESEKDYPSTWAAITAIAPKIGCTPETLRAWHQKHLDQQNPIKVQQISDQEKMKQMEREIKELKRANEILRKAAAFFRLGGA
RPPTQIMVDFIHNNKDLYGVDAICRILPIAASTYYRTLDLADNPEHRAKRDLHDEHHAEQIKRIWKESLGRYGVRKVWQKLKREGYVIGRCTVARLMQKL
DIQGVWRGKNKQTTRNRDDQKRADDLVKRNFSADRPDQLWVSDFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALHDRGMPKNVIH
HSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHSALGYVSPFEFEAMYYDKIN
PLGQVA
RPPTQIMVDFIHNNKDLYGVDAICRILPIAASTYYRTLDLADNPEHRAKRDLHDEHHAEQIKRIWKESLGRYGVRKVWQKLKREGYVIGRCTVARLMQKL
DIQGVWRGKNKQTTRNRDDQKRADDLVKRNFSADRPDQLWVSDFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALHDRGMPKNVIH
HSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHSALGYVSPFEFEAMYYDKIN
PLGQVA
Blast result :
Comments
ISAlw5 is 96% aa (transposase) similar to ISAba19.
The third ORF is the putative ORFAB reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB reconstructed in silico by possible -1 frameshift.
References