ISAlw4
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| KX426227 | ND | Acinetobacter lwoffii | Acinetobacter lwoffii Acinetobacter lwoffii M2a Acinetobacter lwoffii ED23-35 pALWED1.1 |
DNA section
IS Length : 1309 bp
Ends
IR Length : 25
IRL : TGAACCGTACCGGGTTTGTCGGAGAGTCAATATTCTGAGAGACTATCCCG
IRR : TGAACCGTACCGGGTTTGTCGGAGACTTTTTTATTTAAGTTAGGCCACCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACCGTTCCATAGAAAAA | TAG | GTACGGTGCTAAAACAC | 3 |
DNA sequence
TGAACCGTACCGGGTTTGTCGGAGAGTCAATATTCTGAGAGACTATCCCGATGACAAAATTAAAATATACCCCTGAAATCAGAGAAAGAGCGGTTCAATT
ATTGATTGAATCTGAAAAAGATTACCCTTCTACTTGGGCTGCAATCACAGCAATTGCGCCTAAGATTGGTTGTACTCCTGAAACACTTCGTGCCTGGCAT
CAAAAGCATTTAGATAAACAAAATCCAGTTAAAGTACAGCAGCTTTCAGACCAAGAACGTATCAAACAACTGGAACGCGAAAATAAAGAACTGCAACGCG
CCAATGAAATTCTACGTAAAGCAGCCGCTTTTTTCGCCCAGGCGGAGCTCGACCGCCCACACAAATAATGGTGGATTTCATCCATAATAATAAAGAGCTG
TACGGAGTCGAGGCGATTTGTAGGATTTTACCGATCGCAGCTTCAACCTATTACCGGACTCTAGATCTCTGCGAAAATCCAGAACATCGAGCAAAGCGAG
ATTTACATGACTTGCATCATGCTGAGGAGATTAAACGAATTTGGAAGGAAAGTTCAGGTCGGTATGGTGTACGTAAAGTCTGGCAACAATTGAAACGTGA
AGGCTATATTATTGCACGCTGTACTGTTGCTCGATTAATGAAAAAGCTAGGTATACAAGGTGTTTGGCGAGGTAAGAATAAACAAACCACCCGTAGCCGA
GATGATCAAAAAAGAGCAGATGATTTAGTGAAACGTAATTTTAATGCTGATCATCCTGACCAACTGTGGGTGAGTGACTTTACGTATATTCAAACTCATT
CAGGCTGGGTCTATACCGCCTTTATTATTGATGTGTTCTCACGAGCAATTGTTGGATGGAAAGTATCTACACGGATGAATACAGATATGGTACTCGATGC
ATTGGAGCAAGCATTGCACGATCGAGGCATGCCAAAGAATGTGATTCATCATTCCGATAGAGGTGTTCAATATCTTTCTATTCGCTATACCAATCGTTTA
GAAGCTGCAAATTTACGAGCATCAGTCGGTACAACGGGTGATTCATACGATAATGCTCTGGCTGAAACGGTGAATGGCTTATACAAAACAGAGGTGATTG
AATATTTAAAAGCAGATTGGCAAGGTTTAGCAGATGTACAACTTGCGACACTAAACTGGGTAGATTGGTTCAACAAAAAGCGTGTACACAGTGCACTGGG
TTATGTATCGCCTTTTGAGTTTGAAGCAATGTACTATGATAAGATTAACCCGTTAGGTCAGGTGGCCTAACTTAAATAAAAAAGTCTCCGACAAACCCGG
TACGGTTCA
ATTGATTGAATCTGAAAAAGATTACCCTTCTACTTGGGCTGCAATCACAGCAATTGCGCCTAAGATTGGTTGTACTCCTGAAACACTTCGTGCCTGGCAT
CAAAAGCATTTAGATAAACAAAATCCAGTTAAAGTACAGCAGCTTTCAGACCAAGAACGTATCAAACAACTGGAACGCGAAAATAAAGAACTGCAACGCG
CCAATGAAATTCTACGTAAAGCAGCCGCTTTTTTCGCCCAGGCGGAGCTCGACCGCCCACACAAATAATGGTGGATTTCATCCATAATAATAAAGAGCTG
TACGGAGTCGAGGCGATTTGTAGGATTTTACCGATCGCAGCTTCAACCTATTACCGGACTCTAGATCTCTGCGAAAATCCAGAACATCGAGCAAAGCGAG
ATTTACATGACTTGCATCATGCTGAGGAGATTAAACGAATTTGGAAGGAAAGTTCAGGTCGGTATGGTGTACGTAAAGTCTGGCAACAATTGAAACGTGA
AGGCTATATTATTGCACGCTGTACTGTTGCTCGATTAATGAAAAAGCTAGGTATACAAGGTGTTTGGCGAGGTAAGAATAAACAAACCACCCGTAGCCGA
GATGATCAAAAAAGAGCAGATGATTTAGTGAAACGTAATTTTAATGCTGATCATCCTGACCAACTGTGGGTGAGTGACTTTACGTATATTCAAACTCATT
CAGGCTGGGTCTATACCGCCTTTATTATTGATGTGTTCTCACGAGCAATTGTTGGATGGAAAGTATCTACACGGATGAATACAGATATGGTACTCGATGC
ATTGGAGCAAGCATTGCACGATCGAGGCATGCCAAAGAATGTGATTCATCATTCCGATAGAGGTGTTCAATATCTTTCTATTCGCTATACCAATCGTTTA
GAAGCTGCAAATTTACGAGCATCAGTCGGTACAACGGGTGATTCATACGATAATGCTCTGGCTGAAACGGTGAATGGCTTATACAAAACAGAGGTGATTG
AATATTTAAAAGCAGATTGGCAAGGTTTAGCAGATGTACAACTTGCGACACTAAACTGGGTAGATTGGTTCAACAAAAAGCGTGTACACAGTGCACTGGG
TTATGTATCGCCTTTTGAGTTTGAAGCAATGTACTATGATAAGATTAACCCGTTAGGTCAGGTGGCCTAACTTAAATAAAAAAGTCTCCGACAAACCCGG
TACGGTTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 319 bp | 105 aa | 50 | 368 | + | No |
Description : First part of the transposase
ORF sequence :
MTKLKYTPEIRERAVQLLIESEKDYPSTWAAITAIAPKIGCTPETLRAWHQKHLDKQNPVKVQQLSDQERIKQLERENKELQRANEILRKAAAFFAQAEL
DRPHK
DRPHK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 952 bp | 316 aa | 319 | 1270 | + | No |
Description : Second part of the transposase
ORF sequence :
SSRFFRPGGARPPTQIMVDFIHNNKELYGVEAICRILPIAASTYYRTLDLCENPEHRAKRDLHDLHHAEEIKRIWKESSGRYGVRKVWQQLKREGYIIAR
CTVARLMKKLGIQGVWRGKNKQTTRSRDDQKRADDLVKRNFNADHPDQLWVSDFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALH
DRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHSALGYVSPFE
FEAMYYDKINPLGQVA
CTVARLMKKLGIQGVWRGKNKQTTRSRDDQKRADDLVKRNFNADHPDQLWVSDFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALH
DRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHSALGYVSPFE
FEAMYYDKINPLGQVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1221 bp | 406 aa | 50 | 1270 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKLKYTPEIRERAVQLLIESEKDYPSTWAAITAIAPKIGCTPETLRAWHQKHLDKQNPVKVQQLSDQERIKQLERENKELQRANEILRKAAAFFRPGGA
RPPTQIMVDFIHNNKELYGVEAICRILPIAASTYYRTLDLCENPEHRAKRDLHDLHHAEEIKRIWKESSGRYGVRKVWQQLKREGYIIARCTVARLMKKL
GIQGVWRGKNKQTTRSRDDQKRADDLVKRNFNADHPDQLWVSDFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALHDRGMPKNVIH
HSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHSALGYVSPFEFEAMYYDKIN
PLGQVA
RPPTQIMVDFIHNNKELYGVEAICRILPIAASTYYRTLDLCENPEHRAKRDLHDLHHAEEIKRIWKESSGRYGVRKVWQQLKREGYIIARCTVARLMKKL
GIQGVWRGKNKQTTRSRDDQKRADDLVKRNFNADHPDQLWVSDFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALHDRGMPKNVIH
HSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHSALGYVSPFEFEAMYYDKIN
PLGQVA
Blast result :
Comments
ISAlw4 is 98% aa (transposase) similar to ISAba34.
The third ORF is the putative ORFAB reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB reconstructed in silico by possible -1 frameshift.
References