ISAlw22
- Family ISNCY
- Group IS1202
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Acinetobacter lwoffii | Acinetobacter lwoffii Acinetobacter lwoffii M2a Acinetobacter lwoffii ED9-5a plasmid pALWED3.6 A. lwoffii ED45-23 plasmid pALWED2.1 |
DNA section
IS Length : 1482 bp
Ends
IR Length : 26
IRL : TGTTAGAACCCATTTAAAGTGTCTATATTCTCACCAATAAAAATGTCTGG
IRR : TGTTATTCACCAATTAAAATGTCTATGCGATAAACCATTTAAAATGTCCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| taatggcgattatacgaaag | gccct | tgtaagtccaaaatagaaat | 5 |
| TAATACACCTTATACGAAATGCGTT | TGTACCAGATGAGCTATTAGATCTA | 0 | |
| TAATAGAGCTTATACGAAACGTATA | CCCAAGGCATGGCTTTTAGTCATGC | 0 | |
| TAATGGAGGTTATGCGAAGT | CGACT | TGTAATGCAATTTGCATTAC | 5 |
| TAATGGCGGTTATACGAAGT | CGACT | TGTAATGCAATTTGCATTAC | 5 |
DNA sequence
TGTTAGAACCCATTTAAAGTGTCTATATTCTCACCAATAAAAATGTCTGGTTTATGATGGTTTTTATCACCATGGACTGGATATAAAATATAGATGCTGA
TCACTATGTCTGACAAAGAAATTCAACGTCTTGCAGTTCTGCAAGACGTTCGAGATCATCGTATTACCCAAGTCCGTGCTGCTGAAATCCTGAATCTTTC
CACCCGTCAAATTACCCGGTTATTGCAGAAGCTCAATCAAGATGGTGTTTCAGGCATGACGCATGCTAGTCGTGGTCAACCGGGTCATCGTCGCCATGAC
GTTCTGTTAAAATCAGAATGTCTTTCCATTATTTCTGAACATCTGCTGGGCTTTGGACCTACCTTGGCGCATGAGAAGCTCAGCAGCATGTTTGACCTGA
ATATCCCGGTAGAAACGGTTCGGCGCTGGATGACTGCAAATGACCTTTGGATTCCGCGATCCAAACGTCTAAAACGCCCATACCAGCCACGCTATAACCG
TGATTGCTTCGGTGAGCTGATCCAGATTGATGGCTCATATCATGACTGGTTTGAAGGAAGAGCCACCAAATGCTGTTTGCTGGTTTATATCGATGATGCT
ACCGGCAAGCTGTTGCATCTACGCTTTTGTGAGGCTGAAACGACCTTTGACTATATGCTTTCAACCCGAGCCTACATTGAGCAATACGGCAAGCCCTTGG
CCTTTTATAGCGATAAACACTCGGTATTTAGAGTCAACCAGAAATCCAGGCAAGACAGCCAGATCACGCAATTTGGGCGGATTCTGAATGAGTTAAACAT
TGATATTATCTTCGCCAATTCACCCCAGGCCAAAGGTCGCGTAGAGCGAGCGAATAGAACCCTTCAGGACCGTTTGATCAAGGAGATGCGCCTGGAAGGC
ATCTGTTCGATTGCAGAAGCAAATGCCTGGTTGCCCTGCTTTATTGAGCAGTTCAATCAGAAGTTCGCCAAATGTGCGCGAAACTCAAAGAACCTGCATC
GACCATTAACCGAATCTGATCTTGAACTGGATGATATTTTTACCTGGCAGGAACCGCGTAAGGTCACCAAGAACCTGACGATTACCTATGACAAATGTAT
TTATCTGCTTGAACCGACAGAGCTGAATCATAAGCTTGTTGGGCAATATATTTCATTTCTGGAGTACCCGGATGGGACTGTGGCCCTCATGCACGAGGGA
CGAAAGCTCAACTACAGCATTTTCAATAAATTAGCTGGGCTACAGCAGAATGAGATCGTTGAGAATAAACGGTTAGGTTCAGTCCTGGCACATATTCAGC
AACAGCATGAAGAGTTGGAAAAACAGAATAAACGTTCCCGTCTCAAGAAAAGTATGCCGAGTCGTAAAGCTCAGAAAGCTGTTATTGAACAAAGAAAGTT
AAATCCTGTGCTTGACTCTTGTGGTTAAAAACAGGACATTTTAAATGGTTTATCGCATAGACATTTTAATTGGTGAATAACA
TCACTATGTCTGACAAAGAAATTCAACGTCTTGCAGTTCTGCAAGACGTTCGAGATCATCGTATTACCCAAGTCCGTGCTGCTGAAATCCTGAATCTTTC
CACCCGTCAAATTACCCGGTTATTGCAGAAGCTCAATCAAGATGGTGTTTCAGGCATGACGCATGCTAGTCGTGGTCAACCGGGTCATCGTCGCCATGAC
GTTCTGTTAAAATCAGAATGTCTTTCCATTATTTCTGAACATCTGCTGGGCTTTGGACCTACCTTGGCGCATGAGAAGCTCAGCAGCATGTTTGACCTGA
ATATCCCGGTAGAAACGGTTCGGCGCTGGATGACTGCAAATGACCTTTGGATTCCGCGATCCAAACGTCTAAAACGCCCATACCAGCCACGCTATAACCG
TGATTGCTTCGGTGAGCTGATCCAGATTGATGGCTCATATCATGACTGGTTTGAAGGAAGAGCCACCAAATGCTGTTTGCTGGTTTATATCGATGATGCT
ACCGGCAAGCTGTTGCATCTACGCTTTTGTGAGGCTGAAACGACCTTTGACTATATGCTTTCAACCCGAGCCTACATTGAGCAATACGGCAAGCCCTTGG
CCTTTTATAGCGATAAACACTCGGTATTTAGAGTCAACCAGAAATCCAGGCAAGACAGCCAGATCACGCAATTTGGGCGGATTCTGAATGAGTTAAACAT
TGATATTATCTTCGCCAATTCACCCCAGGCCAAAGGTCGCGTAGAGCGAGCGAATAGAACCCTTCAGGACCGTTTGATCAAGGAGATGCGCCTGGAAGGC
ATCTGTTCGATTGCAGAAGCAAATGCCTGGTTGCCCTGCTTTATTGAGCAGTTCAATCAGAAGTTCGCCAAATGTGCGCGAAACTCAAAGAACCTGCATC
GACCATTAACCGAATCTGATCTTGAACTGGATGATATTTTTACCTGGCAGGAACCGCGTAAGGTCACCAAGAACCTGACGATTACCTATGACAAATGTAT
TTATCTGCTTGAACCGACAGAGCTGAATCATAAGCTTGTTGGGCAATATATTTCATTTCTGGAGTACCCGGATGGGACTGTGGCCCTCATGCACGAGGGA
CGAAAGCTCAACTACAGCATTTTCAATAAATTAGCTGGGCTACAGCAGAATGAGATCGTTGAGAATAAACGGTTAGGTTCAGTCCTGGCACATATTCAGC
AACAGCATGAAGAGTTGGAAAAACAGAATAAACGTTCCCGTCTCAAGAAAAGTATGCCGAGTCGTAAAGCTCAGAAAGCTGTTATTGAACAAAGAAAGTT
AAATCCTGTGCTTGACTCTTGTGGTTAAAAACAGGACATTTTAAATGGTTTATCGCATAGACATTTTAATTGGTGAATAACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1335 bp | 444 aa | 94 | 1428 | + | No |
Chemistry : DDE
ORF sequence :
MLITMSDKEIQRLAVLQDVRDHRITQVRAAEILNLSTRQITRLLQKLNQDGVSGMTHASRGQPGHRRHDVLLKSECLSIISEHLLGFGPTLAHEKLSSMF
DLNIPVETVRRWMTANDLWIPRSKRLKRPYQPRYNRDCFGELIQIDGSYHDWFEGRATKCCLLVYIDDATGKLLHLRFCEAETTFDYMLSTRAYIEQYGK
PLAFYSDKHSVFRVNQKSRQDSQITQFGRILNELNIDIIFANSPQAKGRVERANRTLQDRLIKEMRLEGICSIAEANAWLPCFIEQFNQKFAKCARNSKN
LHRPLTESDLELDDIFTWQEPRKVTKNLTITYDKCIYLLEPTELNHKLVGQYISFLEYPDGTVALMHEGRKLNYSIFNKLAGLQQNEIVENKRLGSVLAH
IQQQHEELEKQNKRSRLKKSMPSRKAQKAVIEQRKLNPVLDSCG
DLNIPVETVRRWMTANDLWIPRSKRLKRPYQPRYNRDCFGELIQIDGSYHDWFEGRATKCCLLVYIDDATGKLLHLRFCEAETTFDYMLSTRAYIEQYGK
PLAFYSDKHSVFRVNQKSRQDSQITQFGRILNELNIDIIFANSPQAKGRVERANRTLQDRLIKEMRLEGICSIAEANAWLPCFIEQFNQKFAKCARNSKN
LHRPLTESDLELDDIFTWQEPRKVTKNLTITYDKCIYLLEPTELNHKLVGQYISFLEYPDGTVALMHEGRKLNYSIFNKLAGLQQNEIVENKRLGSVLAH
IQQQHEELEKQNKRSRLKKSMPSRKAQKAVIEQRKLNPVLDSCG
Blast result :
Comments
ISAlw22 is 98% aa similar to ISAba32.
Three and one copies of ISAlw22 occur in A. lwoffii plasmids pALWED3.6 and pALWED2.1, respectively. Two copies have no direct repeat in pALWED3.6. The third one is flanked by 5-bp DR and located at the same position as the single copy of pALWED2.1.
Three and one copies of ISAlw22 occur in A. lwoffii plasmids pALWED3.6 and pALWED2.1, respectively. Two copies have no direct repeat in pALWED3.6. The third one is flanked by 5-bp DR and located at the same position as the single copy of pALWED2.1.
References