ISAlw18
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Acinetobacter lwoffii | Acinetobacter lwoffii Acinetobacter lwoffii M2a |
DNA section
IS Length : 1078 bp
Ends
IR Length : 24
IRL : agttgacaaaaataactaatgatgcgaaaaggtttttttagttaacagat
IRR : agtatccattaaaaagtaaacatgtattcttttccgaattggtttgatac
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| gaacttctatttaataacga | TA | aatgtcacactttgtttaaa | 2 |
| aaactaaaataatcttcaca | TA | gcactataactaaaatatct | 2 |
| atagataaagcgaaagataa | TA | ttgctatggtatctgttgaa | 2 |
| agtcttcaggagacactgca | TA | agagtttaaagaaataaata | 2 |
DNA sequence
AGTTGACAAAAATAACTAATGATGCGAAAAGGTTTTTTTAGTTAACAGATGTTCACACATGCCTAGAATTGTTTCTGTACCACTTAGTTTAGAACAGCGA
GAACGACTCATTTTTCTGGTCAAGCATGCAAAACATTGGCGTGAAAGGCAACGTGCCCAAACTATTCTCTGGCTTTCCGAAGGTAAAAGTGTTGCGGAAG
TTGCCACTCTACAAGAACGTATCCCAGAAACCATAAGGCTACAACGCCGACGTTGGGAATTGTATGAGCTTGAATCGATTAAAGAAGGTCATCGCTCAGG
TCGACCCAATACATTGATCTCAGACTATCAGGCGAAAATCCTAGATTGGGTCAATACAAGTCCACTCAATGCAGAACAAATCCGAGTCAAACTACATGAA
GAATACGAAGTGTCCGTGTCTGTTGAAACCATTCGTAAGTTTTTACGTGATTCAGGTATGGTCTTCAAGCGTACCCGTCATAGCTTGAAAAAAAAGAGAT
CCGATTGCATTTGAACAAGCAACACAGCAGATTGAAGAATTACGAGAGCAAGCGGCACGTGGTGAAATTATATTAGGTTATGTTGATGAAACAGGATTTT
CATCTACACCTGATAACCGCTATGCGTGGACAAAGATAGGTGATGTTCATGCGGTTGATGCAATAAGATTAAAACGAGTTAATGTCATGGGGTGTTTGCT
TTCAACGGGGAAACTGGTGACTAGTTGTTTACAAGAGTCAGTGACAAGCACTTGGTTTTATGCCTATTTAACTGGAATAGCTCAACAAGTAAAACAGACA
TATAACCTTCCATTGGTTCTTATTGTTGATAATGCCTCGATTCATAGAAGTAAAAAGATGGCTGATTACAGAGAGCTACTTAAAACTAACTTTTCGACGA
ATCTGTATTTTATTCCAGCTTATAGCCCTGAGTTAAATCGAATCGAGATGGTATGGAAGCAGATGAAATATTATTGGCGAGATTTTCAAGTCATGACAGC
TGACAAAATAGAGCAATGGGTGGAGAAAGTATCAAACCAATTCGGAAAAGAATACATGTTTACTTTTTAATGGATACT
GAACGACTCATTTTTCTGGTCAAGCATGCAAAACATTGGCGTGAAAGGCAACGTGCCCAAACTATTCTCTGGCTTTCCGAAGGTAAAAGTGTTGCGGAAG
TTGCCACTCTACAAGAACGTATCCCAGAAACCATAAGGCTACAACGCCGACGTTGGGAATTGTATGAGCTTGAATCGATTAAAGAAGGTCATCGCTCAGG
TCGACCCAATACATTGATCTCAGACTATCAGGCGAAAATCCTAGATTGGGTCAATACAAGTCCACTCAATGCAGAACAAATCCGAGTCAAACTACATGAA
GAATACGAAGTGTCCGTGTCTGTTGAAACCATTCGTAAGTTTTTACGTGATTCAGGTATGGTCTTCAAGCGTACCCGTCATAGCTTGAAAAAAAAGAGAT
CCGATTGCATTTGAACAAGCAACACAGCAGATTGAAGAATTACGAGAGCAAGCGGCACGTGGTGAAATTATATTAGGTTATGTTGATGAAACAGGATTTT
CATCTACACCTGATAACCGCTATGCGTGGACAAAGATAGGTGATGTTCATGCGGTTGATGCAATAAGATTAAAACGAGTTAATGTCATGGGGTGTTTGCT
TTCAACGGGGAAACTGGTGACTAGTTGTTTACAAGAGTCAGTGACAAGCACTTGGTTTTATGCCTATTTAACTGGAATAGCTCAACAAGTAAAACAGACA
TATAACCTTCCATTGGTTCTTATTGTTGATAATGCCTCGATTCATAGAAGTAAAAAGATGGCTGATTACAGAGAGCTACTTAAAACTAACTTTTCGACGA
ATCTGTATTTTATTCCAGCTTATAGCCCTGAGTTAAATCGAATCGAGATGGTATGGAAGCAGATGAAATATTATTGGCGAGATTTTCAAGTCATGACAGC
TGACAAAATAGAGCAATGGGTGGAGAAAGTATCAAACCAATTCGGAAAAGAATACATGTTTACTTTTTAATGGATACT
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 456 bp | 151 aa | 59 | 514 | + | No |
Description : First part of the transposase
ORF sequence :
MPRIVSVPLSLEQRERLIFLVKHAKHWRERQRAQTILWLSEGKSVAEVATLQERIPETIRLQRRRWELYELESIKEGHRSGRPNTLISDYQAKILDWVNT
SPLNAEQIRVKLHEEYEVSVSVETIRKFLRDSGMVFKRTRHSLKKKRSDCI
SPLNAEQIRVKLHEEYEVSVSVETIRKFLRDSGMVFKRTRHSLKKKRSDCI
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 582 bp | 193 aa | 489 | 1070 | + | No |
Description : Second part of the transposase
ORF sequence :
KKRDPIAFEQATQQIEELREQAARGEIILGYVDETGFSSTPDNRYAWTKIGDVHAVDAIRLKRVNVMGCLLSTGKLVTSCLQESVTSTWFYAYLTGIAQQ
VKQTYNLPLVLIVDNASIHRSKKMADYRELLKTNFSTNLYFIPAYSPELNRIEMVWKQMKYYWRDFQVMTADKIEQWVEKVSNQFGKEYMFTF
VKQTYNLPLVLIVDNASIHRSKKMADYRELLKTNFSTNLYFIPAYSPELNRIEMVWKQMKYYWRDFQVMTADKIEQWVEKVSNQFGKEYMFTF
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1012 bp | 336 aa | 59 | 1070 | + | Yes |
Chemistry : DDE
ORF sequence :
MPRIVSVPLSLEQRERLIFLVKHAKHWRERQRAQTILWLSEGKSVAEVATLQERIPETIRLQRRRWELYELESIKEGHRSGRPNTLISDYQAKILDWVNT
SPLNAEQIRVKLHEEYEVSVSVETIRKFLRDSGMVFKRTRHSLKKRDPIAFEQATQQIEELREQAARGEIILGYVDETGFSSTPDNRYAWTKIGDVHAVD
AIRLKRVNVMGCLLSTGKLVTSCLQESVTSTWFYAYLTGIAQQVKQTYNLPLVLIVDNASIHRSKKMADYRELLKTNFSTNLYFIPAYSPELNRIEMVWK
QMKYYWRDFQVMTADKIEQWVEKVSNQFGKEYMFTF
SPLNAEQIRVKLHEEYEVSVSVETIRKFLRDSGMVFKRTRHSLKKRDPIAFEQATQQIEELREQAARGEIILGYVDETGFSSTPDNRYAWTKIGDVHAVD
AIRLKRVNVMGCLLSTGKLVTSCLQESVTSTWFYAYLTGIAQQVKQTYNLPLVLIVDNASIHRSKKMADYRELLKTNFSTNLYFIPAYSPELNRIEMVWK
QMKYYWRDFQVMTADKIEQWVEKVSNQFGKEYMFTF
Blast result :
Comments
ISAlw18 is 50% aa (transposase) similar to ISAcma15.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
One of the 5 ISAlw18 copies in strain M2a has a divergent IRR (agttgtcattaaaaagtaaacatg). Acinetobacter wuhouensis strain WCHAW010062 chromosome (CP033133.1) contains 27 intact and 1 interrupted copies of this variant.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
One of the 5 ISAlw18 copies in strain M2a has a divergent IRR (agttgtcattaaaaagtaaacatg). Acinetobacter wuhouensis strain WCHAW010062 chromosome (CP033133.1) contains 27 intact and 1 interrupted copies of this variant.
References