ISAlw17
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Acinetobacter lwoffii | Acinetobacter lwoffii Acinetobacter lwoffii M2a |
DNA section
IS Length : 877 bp
Ends
IR Length : 15
IRL : TATAGCCAAATACTTAAAAATATAGAACAATAGAAACATGGGATATTCAA
IRR : TATAGCTAAATACTAAATAAATCATTACAGTAATCTTTAAACAATTTATC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| attcacgaatttctgtgtca | TA | gctacagaattgtgctgtat | 0 |
| gctataccaatatcacaaac | TA | gttcactattcttctgagaa | 0 |
DNA sequence
TATAGCCAAATACTTAAAAATATAGAACAATAGAAACATGGGATATTCAAAAGATTTTAAAGACAAAGTCATAGAAATTATGACTAGAGATAAAATGTCT
GTGCGAAAAGCAGCACAGCATTTTAACGTCTGTATACAGACGATTCAGAATTGGAAAAAGTCCACTGTAACTAAACCTATTCCTGGTCGCCCAGCTAAAA
TTAGCAAAGAGCAAATATTGAATGATGTTGAACAATATCCCGATGATTACATCAGTGAACGGGCAGAACGTTTTGGTGTCAGTACACATGCAGTATTTAA
TGCGCTACATGCTGCAGGAATATCGCGTAAAAAAAGACGTTAAAGCACCCTAAAGCAGATCAAAATCAACGTCGGCAATTTCAGAACTTGATTGAATGTT
TAGAACAACAATTACCACTGGTTTATATCGATGAAAGTGGTTTTCAAAGCGAAAGTACGAGATCGCATAGTTATAGCCCTAAAGGAGCGCGAGCTGAAGC
TGAATTTAACTGGCAGTTAAAAAATTGTAGCAATGCGATTGGCGCATTAATCGAAAATAGATTGCTTACAGTTGGAGTATTTGATTGTTCAGTGAATACC
GAGGTATTTGAATGCTGGATTGAACAGGATTTATTGCCAAAGCTGAAAAAAACCAGTGTATTGATTATGGATAATGCCACCTTTCACAAAGGAACAAGGC
TTCAGGAGTTGATACGTTGTGCAGGCCATTACATTGTGTGGCTTCCTAAATATAGCCCGGACTTAAATCCTATAGAAAAAATGTGGTCACGAGTAAAAAT
GATTCGCAATAAATTTAGAGTTAAAGATATTGATAAATTGTTTAAAGATTACTGTAATGATTTATTTAGTATTTAGCTATA
GTGCGAAAAGCAGCACAGCATTTTAACGTCTGTATACAGACGATTCAGAATTGGAAAAAGTCCACTGTAACTAAACCTATTCCTGGTCGCCCAGCTAAAA
TTAGCAAAGAGCAAATATTGAATGATGTTGAACAATATCCCGATGATTACATCAGTGAACGGGCAGAACGTTTTGGTGTCAGTACACATGCAGTATTTAA
TGCGCTACATGCTGCAGGAATATCGCGTAAAAAAAGACGTTAAAGCACCCTAAAGCAGATCAAAATCAACGTCGGCAATTTCAGAACTTGATTGAATGTT
TAGAACAACAATTACCACTGGTTTATATCGATGAAAGTGGTTTTCAAAGCGAAAGTACGAGATCGCATAGTTATAGCCCTAAAGGAGCGCGAGCTGAAGC
TGAATTTAACTGGCAGTTAAAAAATTGTAGCAATGCGATTGGCGCATTAATCGAAAATAGATTGCTTACAGTTGGAGTATTTGATTGTTCAGTGAATACC
GAGGTATTTGAATGCTGGATTGAACAGGATTTATTGCCAAAGCTGAAAAAAACCAGTGTATTGATTATGGATAATGCCACCTTTCACAAAGGAACAAGGC
TTCAGGAGTTGATACGTTGTGCAGGCCATTACATTGTGTGGCTTCCTAAATATAGCCCGGACTTAAATCCTATAGAAAAAATGTGGTCACGAGTAAAAAT
GATTCGCAATAAATTTAGAGTTAAAGATATTGATAAATTGTTTAAAGATTACTGTAATGATTTATTTAGTATTTAGCTATA
Recoding section
- Recoding by frameshift
- Frame +1
- Type transcriptional
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 406 bp | 101 aa | 36 | 441 | + | No |
Description : First part of the transposase
ORF sequence :
MGYSKDFKDKVIEIMTRDKMSVRKAAQHFNVCIQTIQNWKKSTVTKPIPGRPAKISKEQILNDVEQYPDDYISERAERFGVSTHAVFNALHAAGISRKKR
R
R
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 546 bp | 181 aa | 329 | 874 | + | No |
Description : Second part of the transposase
ORF sequence :
KKTLKHPKADQNQRRQFQNLIECLEQQLPLVYIDESGFQSESTRSHSYSPKGARAEAEFNWQLKNCSNAIGALIENRLLTVGVFDCSVNTEVFECWIEQD
LLPKLKKTSVLIMDNATFHKGTRLQELIRCAGHYIVWLPKYSPDLNPIEKMWSRVKMIRNKFRVKDIDKLFKDYCNDLFSI
LLPKLKKTSVLIMDNATFHKGTRLQELIRCAGHYIVWLPKYSPDLNPIEKMWSRVKMIRNKFRVKDIDKLFKDYCNDLFSI
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 839 bp | 278 aa | 36 | 874 | + | Yes |
Chemistry : DDE
ORF sequence :
MGYSKDFKDKVIEIMTRDKMSVRKAAQHFNVCIQTIQNWKKSTVTKPIPGRPAKISKEQILNDVEQYPDDYISERAERFGVSTHAVFNALHAAGISRKKT
LKHPKADQNQRRQFQNLIECLEQQLPLVYIDESGFQSESTRSHSYSPKGARAEAEFNWQLKNCSNAIGALIENRLLTVGVFDCSVNTEVFECWIEQDLLP
KLKKTSVLIMDNATFHKGTRLQELIRCAGHYIVWLPKYSPDLNPIEKMWSRVKMIRNKFRVKDIDKLFKDYCNDLFSI
LKHPKADQNQRRQFQNLIECLEQQLPLVYIDESGFQSESTRSHSYSPKGARAEAEFNWQLKNCSNAIGALIENRLLTVGVFDCSVNTEVFECWIEQDLLP
KLKKTSVLIMDNATFHKGTRLQELIRCAGHYIVWLPKYSPDLNPIEKMWSRVKMIRNKFRVKDIDKLFKDYCNDLFSI
Blast result :
Comments
ISAlw17 is 63% aa (transposase) similar to ISAba44.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible +1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible +1 frameshift.
References