ISAlw16
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Acinetobacter lwoffii | Acinetobacter lwoffii Acinetobacter lwoffii M2a |
DNA section
IS Length : 2512 bp
Ends
IR Length : 19
IRL : GTAAGCGTCCGCTGAACACACCTTATGAATTCATCCTATAAGCCTTAATT
IRR : GTAAGCGTCCGCTGAACCCCATGCCTATTTTTTAATTCGGTTCAGGTTTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| aaagaaaacgaatagccaat | cgaacaat | gaccatatggactggtattt | 8 |
| aaatttcatgcatttatcat | gaaatatc | attatatttttcacttattt | 8 |
| tttttctgaaaatataatag | actggttt | tcactcaaagttccacttaa | 8 |
| tttataagctttgttacaaa | gttaatcc | atctaattaaatgcgcttta | 8 |
DNA sequence
GTAAGCGTCCGCTGAACACACCTTATGAATTCATCCTATAAGCCTTAATTTAGTCCCCACTTAAAAACAAGTGGGGACTAAAATTTATGACTACAAATCA
CCAGACATCCATCGCATCTCTTGCGAAAAAACGAAGAACATACAGTGCTGAATTTAAACAGCAGATCGTTCAGGCTTGTAAAGCACCAGACGTTTCAATT
GCTTCAGTCGCTTTGCAACATGGATTGAATACAAATCTTGTATCCAAATGGATTCGCTTAATTGATGCTAAGCCAGGGAATGATCGCTCACCACTACCGA
ATAAACCTGCATTTATTGCCTTATCTTGCTCTGCACCATTAGATCCTACTCCTACTGACATGTTAAAGGTTCAAATTACTTTACCCCACTCAAAAGCAGA
AATTGGCTTGAAATGGCAAGTATCAGAAATATCTGCTTTAGCAGAATTACTCAAGGCACTTGCAACATGATCCGCATTGATGAAATCTGGTTGTCTACTC
AGCCCATGGACATGCGTGCAGGTATGGATACGACCATGGCTCAGGTGGTGAGAGCCTTTGGCTACATCAAACCGCATTGTGCTTACCTGTTCTGTAATAA
ACGTGGCCATCGCATGAAAGTACTGGTGCATGATGGACTGGGCATCTGGCTGTGTGCCCGGCGGCTGGAACAGGGCAAATTTCACTGGGCTCAAGTTCAC
CAGGGTGAAAGCGTAGCTCTCAGCCCGGAACAGTTACAGGCACTGATCCAGGGTTTGCCCTGGCAGCGCATTGGACGACAGCAGGTGGTGACGATGCTTT
AAACCAAGCTGTTCCATTCTGCTATTCTCCAAACGTTCTATTTCATTCTTCTCATGACCTCAGGCATACTGCGGTCATGAATACGCTGCCTGACTTAAGC
CAACTGACCCATGAACAACTGCTGGAATTTACCAGGCAGTTGGCGCTGCAGCATCAGTCTCTGGCACAATCAAATCAAGAATTAGAAAAATCAAACCAGC
AATTGGATGCCAAAGTTCAGCATCTTTCTATTCTCAATCAAAAATACGAGCATGAACTGGCGCTGTTTAAAAAGCACAAATTCGGCAGTAAAAACGAACA
TCTCACCGCAAAACAAATCCATCTCTGGGATGAAGCGGTCGAAGAAGATATCGCAGCCGTTGATCTGGAACTGGAACGGCTGAATGCAGATAAAACCGAT
GCAGCGACACACAAAGCCAAAGCAAATAAACCTAAACGTCGACCACTGCCAGATCATCTACACACCATCCGTATTGAGCATGAACCTGCATCAACCCAAT
GCGCTTGTGGCTGCCAACTCCGTCGTATCGGCGAAGATGTCAGTGAAAAACTGCATTTCAGACCGGCACAGTTCTATAAGGAACAGCATGTGCGTGGTAA
ATGGGTCTGTGATCAGTGTGACACCCTGACTCAGCAAGCGATGCCTGCCTATGTAATTGATAAAGGCATTGCTTCACCTGAATTGCTCAGCCATGTGCTG
GTATCGAAATATGCCGATCATTTGCCGCTGTACCGTCAACGTCTGATCTATCAGCGGGCGGGAATCGAACTTTCTAGATCAACTTTATCTGACTGGATAG
GTCGCTGCGGTGTAGAACTGGAACCTCTGGCCAATGCCTTAAAACAGGTGGTGCTGCAACAGCGGGTGATCCATGCCGATGAAACGCCGGTCACCATCAT
GCGGATGGGTGATGATGAGAAAAAACCGAAAAAAGGTTATGTCTGGGCCTATGCCACTACACAGTACAATCCAGTTCAGGCAGTGATCTATGACTTTCAG
GATAGTCGTTCAGGCCAGCATGCTGAAGCGTTCTTGAAAGGCTGGCAGGGCAATCTAGTCTGTGATGATTACAGTGGTTATAAAGCACGCTTTAAATCAG
GTCAGGTCATAGAGGTGGGCTGCATGGCCCATGCACGTCGTAAATTCCATGAACTGCATGTGACCGGGAAAAGTCAGGTCGCTGAACAGGCATTGGTGCT
GATTCAGAAACTGTATGCGATAGAAGTAGAACTCAGGAAAAAGACCGATGGTACAGCAGAACAGCGCCGCGAATACCGACAACAGCATAGTCAACCAGTG
ATGCAACAACTGTATGAATGGCTCAACCAACATCATCTGACAGTGCCATCGAGTTCTCCCACCGCCAAGGCGATCAATTACAGCCTAAAACGTTGGCAGG
CACTCTGCCGCTATCTGGATGATGGTAATTTGCCTATAGATAATAACTGGATTGAAAATCAGATGCGTCCCTGGGCGTTGGGGCGCAAGAACTGGCTGTT
TGCTGGCTCGCTGCGCAGTGGTCAGCGAGCGGCAAACATCATGACGTTAATCCAGTCAGCAAAGCTGAATGGGCTGGATCCGTATGCCTATTTAAGTGAT
GTGCTGAAAAGGCTGCCGACACATAAAGTGACCCAGATTGAAGAGTTACTGCCACACTGCTGGAAACCTGAACCGAATTAAAAAATAGGCATGGGGTTCA
GCGGACGCTTAC
CCAGACATCCATCGCATCTCTTGCGAAAAAACGAAGAACATACAGTGCTGAATTTAAACAGCAGATCGTTCAGGCTTGTAAAGCACCAGACGTTTCAATT
GCTTCAGTCGCTTTGCAACATGGATTGAATACAAATCTTGTATCCAAATGGATTCGCTTAATTGATGCTAAGCCAGGGAATGATCGCTCACCACTACCGA
ATAAACCTGCATTTATTGCCTTATCTTGCTCTGCACCATTAGATCCTACTCCTACTGACATGTTAAAGGTTCAAATTACTTTACCCCACTCAAAAGCAGA
AATTGGCTTGAAATGGCAAGTATCAGAAATATCTGCTTTAGCAGAATTACTCAAGGCACTTGCAACATGATCCGCATTGATGAAATCTGGTTGTCTACTC
AGCCCATGGACATGCGTGCAGGTATGGATACGACCATGGCTCAGGTGGTGAGAGCCTTTGGCTACATCAAACCGCATTGTGCTTACCTGTTCTGTAATAA
ACGTGGCCATCGCATGAAAGTACTGGTGCATGATGGACTGGGCATCTGGCTGTGTGCCCGGCGGCTGGAACAGGGCAAATTTCACTGGGCTCAAGTTCAC
CAGGGTGAAAGCGTAGCTCTCAGCCCGGAACAGTTACAGGCACTGATCCAGGGTTTGCCCTGGCAGCGCATTGGACGACAGCAGGTGGTGACGATGCTTT
AAACCAAGCTGTTCCATTCTGCTATTCTCCAAACGTTCTATTTCATTCTTCTCATGACCTCAGGCATACTGCGGTCATGAATACGCTGCCTGACTTAAGC
CAACTGACCCATGAACAACTGCTGGAATTTACCAGGCAGTTGGCGCTGCAGCATCAGTCTCTGGCACAATCAAATCAAGAATTAGAAAAATCAAACCAGC
AATTGGATGCCAAAGTTCAGCATCTTTCTATTCTCAATCAAAAATACGAGCATGAACTGGCGCTGTTTAAAAAGCACAAATTCGGCAGTAAAAACGAACA
TCTCACCGCAAAACAAATCCATCTCTGGGATGAAGCGGTCGAAGAAGATATCGCAGCCGTTGATCTGGAACTGGAACGGCTGAATGCAGATAAAACCGAT
GCAGCGACACACAAAGCCAAAGCAAATAAACCTAAACGTCGACCACTGCCAGATCATCTACACACCATCCGTATTGAGCATGAACCTGCATCAACCCAAT
GCGCTTGTGGCTGCCAACTCCGTCGTATCGGCGAAGATGTCAGTGAAAAACTGCATTTCAGACCGGCACAGTTCTATAAGGAACAGCATGTGCGTGGTAA
ATGGGTCTGTGATCAGTGTGACACCCTGACTCAGCAAGCGATGCCTGCCTATGTAATTGATAAAGGCATTGCTTCACCTGAATTGCTCAGCCATGTGCTG
GTATCGAAATATGCCGATCATTTGCCGCTGTACCGTCAACGTCTGATCTATCAGCGGGCGGGAATCGAACTTTCTAGATCAACTTTATCTGACTGGATAG
GTCGCTGCGGTGTAGAACTGGAACCTCTGGCCAATGCCTTAAAACAGGTGGTGCTGCAACAGCGGGTGATCCATGCCGATGAAACGCCGGTCACCATCAT
GCGGATGGGTGATGATGAGAAAAAACCGAAAAAAGGTTATGTCTGGGCCTATGCCACTACACAGTACAATCCAGTTCAGGCAGTGATCTATGACTTTCAG
GATAGTCGTTCAGGCCAGCATGCTGAAGCGTTCTTGAAAGGCTGGCAGGGCAATCTAGTCTGTGATGATTACAGTGGTTATAAAGCACGCTTTAAATCAG
GTCAGGTCATAGAGGTGGGCTGCATGGCCCATGCACGTCGTAAATTCCATGAACTGCATGTGACCGGGAAAAGTCAGGTCGCTGAACAGGCATTGGTGCT
GATTCAGAAACTGTATGCGATAGAAGTAGAACTCAGGAAAAAGACCGATGGTACAGCAGAACAGCGCCGCGAATACCGACAACAGCATAGTCAACCAGTG
ATGCAACAACTGTATGAATGGCTCAACCAACATCATCTGACAGTGCCATCGAGTTCTCCCACCGCCAAGGCGATCAATTACAGCCTAAAACGTTGGCAGG
CACTCTGCCGCTATCTGGATGATGGTAATTTGCCTATAGATAATAACTGGATTGAAAATCAGATGCGTCCCTGGGCGTTGGGGCGCAAGAACTGGCTGTT
TGCTGGCTCGCTGCGCAGTGGTCAGCGAGCGGCAAACATCATGACGTTAATCCAGTCAGCAAAGCTGAATGGGCTGGATCCGTATGCCTATTTAAGTGAT
GTGCTGAAAAGGCTGCCGACACATAAAGTGACCCAGATTGAAGAGTTACTGCCACACTGCTGGAAACCTGAACCGAATTAAAAAATAGGCATGGGGTTCA
GCGGACGCTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 384 bp | 127 aa | 87 | 470 | + | No |
AG : IS66 TnpA
ORF sequence :
MTTNHQTSIASLAKKRRTYSAEFKQQIVQACKAPDVSIASVALQHGLNTNLVSKWIRLIDAKPGNDRSPLPNKPAFIALSCSAPLDPTPTDMLKVQITLP
HSKAEIGLKWQVSEISALAELLKALAT
HSKAEIGLKWQVSEISALAELLKALAT
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 390 bp | 129 aa | 413 | 802 | + | No |
AG : IS66 TnpB
ORF sequence :
MASIRNICFSRITQGTCNMIRIDEIWLSTQPMDMRAGMDTTMAQVVRAFGYIKPHCAYLFCNKRGHRMKVLVHDGLGIWLCARRLEQGKFHWAQVHQGES
VALSPEQLQALIQGLPWQRIGRQQVVTML
VALSPEQLQALIQGLPWQRIGRQQVVTML
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1605 bp | 534 aa | 877 | 2481 | + | Yes |
Chemistry : DDE
ORF sequence :
MNTLPDLSQLTHEQLLEFTRQLALQHQSLAQSNQELEKSNQQLDAKVQHLSILNQKYEHELALFKKHKFGSKNEHLTAKQIHLWDEAVEEDIAAVDLELE
RLNADKTDAATHKAKANKPKRRPLPDHLHTIRIEHEPASTQCACGCQLRRIGEDVSEKLHFRPAQFYKEQHVRGKWVCDQCDTLTQQAMPAYVIDKGIAS
PELLSHVLVSKYADHLPLYRQRLIYQRAGIELSRSTLSDWIGRCGVELEPLANALKQVVLQQRVIHADETPVTIMRMGDDEKKPKKGYVWAYATTQYNPV
QAVIYDFQDSRSGQHAEAFLKGWQGNLVCDDYSGYKARFKSGQVIEVGCMAHARRKFHELHVTGKSQVAEQALVLIQKLYAIEVELRKKTDGTAEQRREY
RQQHSQPVMQQLYEWLNQHHLTVPSSSPTAKAINYSLKRWQALCRYLDDGNLPIDNNWIENQMRPWALGRKNWLFAGSLRSGQRAANIMTLIQSAKLNGL
DPYAYLSDVLKRLPTHKVTQIEELLPHCWKPEPN
RLNADKTDAATHKAKANKPKRRPLPDHLHTIRIEHEPASTQCACGCQLRRIGEDVSEKLHFRPAQFYKEQHVRGKWVCDQCDTLTQQAMPAYVIDKGIAS
PELLSHVLVSKYADHLPLYRQRLIYQRAGIELSRSTLSDWIGRCGVELEPLANALKQVVLQQRVIHADETPVTIMRMGDDEKKPKKGYVWAYATTQYNPV
QAVIYDFQDSRSGQHAEAFLKGWQGNLVCDDYSGYKARFKSGQVIEVGCMAHARRKFHELHVTGKSQVAEQALVLIQKLYAIEVELRKKTDGTAEQRREY
RQQHSQPVMQQLYEWLNQHHLTVPSSSPTAKAINYSLKRWQALCRYLDDGNLPIDNNWIENQMRPWALGRKNWLFAGSLRSGQRAANIMTLIQSAKLNGL
DPYAYLSDVLKRLPTHKVTQIEELLPHCWKPEPN
Blast result :
Comments
ISAlw16 is 95% aa (transposase)similar to ISAba49.
ISAlw16 has at least four slightly different copies in A. lwoffii M2a. The strain also contains several further IS copies that are related to ISAlw16, but are closer to ISAba46 or ISAba25. The prototype of ISAlw16 was defined according to the copy located on plasmid pAVAci127 of M2a.
ISAlw16 has at least four slightly different copies in A. lwoffii M2a. The strain also contains several further IS copies that are related to ISAlw16, but are closer to ISAba46 or ISAba25. The prototype of ISAlw16 was defined according to the copy located on plasmid pAVAci127 of M2a.
References