ISAlw32
- Family IS5
- Group ISL2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP019143.1 | ND | Acinetobacter lwoffii | Acinetobacter lwoffii Acinetobacter lwoffii ZS207 Acinetobacter lwoffii NCTC5866 |
DNA section
IS Length : 916 bp
Ends
IR Length : 19
IRL : AGGCCTCTTTCATAAATCAAAAAACAAACAGTCTCTTGCTGATTATTTGT
IRR : AGACTTATTTCATAAGTCATTTTTTACTCAGTTCCATATTGTAGATTCCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ttctagttacagggaagtag | agt | gaatgctctataaaaagaaa | 3 |
| tctatgagtaagcctattac | ttt | aaatcattataaaattttat | 3 |
DNA sequence
AGGCCTCTTTCATAAATCAAAAAACAAACAGTCTCTTGCTGATTATTTGTACCGAAGAATTTTAAATATTCATGCATAATCTGCGGATGAAATACATCGA
TTCAAAGAAGCTTTCTGAAACACAGTTTAAGCGATACACAGGCATCTCATGGTCAACCTTTGATTTAATGGTGGAACAACTGAAAATGCATGTTCCTGCT
AAAGGTAGACCCACCAAATTAAGCATAGAAGATCAAATCCTTCTGTGCTTAAGTTATTGGCGTGAATATCGAACATTGTTCCATGTTGCAACTAGTTATG
GTGTGTCAGAGCCGACTGCTTCAAGAATTGTCCGTCATGTAGAGGACTGCCTGATCAAGTCTAATCTATTTAATTTACCGAAGCATTTACCTAAGGGCGA
AGGCATCGACTGGAACGTGGTGATTGTTGATGCCACAGAAATTCCAATACAAAGACCTAAAAAAACAGAAGAAAAGCTATAGTGGCAAAAAGAAGGCACA
TACTTTCAAAGTACAGGCCATCATTCATTATAGAACTCAACAAATTCTGAGTTTATGCACGAGTCGTGGTGCTGTACATGATTTCGCACTCTTCAAACGA
AACTTGCATCAGATTCCGGCAGGTGCCTTTATCCTTGCAGATAAAGGCTATCAAGGGATTTACTCAGTGTATCCAAATAGCCTATTGCCATTAAAAGCAA
AAAAGCGCTGTAAATTGAATCCTGAACTAAAAATCTATAATCAAGAAATCAACAAAAGAAGAATTGGGATTGAGCATGTATTTGGCAGTTTAAAAACCTT
CAAAATCCTTGCTGAGCGATATCGTAATCGAGGAAAAAGACTGGGTTTAAGATTCAATTTAATTGCTGGAATCTACAATATGGAACTGAGTAAAAAATGA
CTTATGAAATAAGTCT
TTCAAAGAAGCTTTCTGAAACACAGTTTAAGCGATACACAGGCATCTCATGGTCAACCTTTGATTTAATGGTGGAACAACTGAAAATGCATGTTCCTGCT
AAAGGTAGACCCACCAAATTAAGCATAGAAGATCAAATCCTTCTGTGCTTAAGTTATTGGCGTGAATATCGAACATTGTTCCATGTTGCAACTAGTTATG
GTGTGTCAGAGCCGACTGCTTCAAGAATTGTCCGTCATGTAGAGGACTGCCTGATCAAGTCTAATCTATTTAATTTACCGAAGCATTTACCTAAGGGCGA
AGGCATCGACTGGAACGTGGTGATTGTTGATGCCACAGAAATTCCAATACAAAGACCTAAAAAAACAGAAGAAAAGCTATAGTGGCAAAAAGAAGGCACA
TACTTTCAAAGTACAGGCCATCATTCATTATAGAACTCAACAAATTCTGAGTTTATGCACGAGTCGTGGTGCTGTACATGATTTCGCACTCTTCAAACGA
AACTTGCATCAGATTCCGGCAGGTGCCTTTATCCTTGCAGATAAAGGCTATCAAGGGATTTACTCAGTGTATCCAAATAGCCTATTGCCATTAAAAGCAA
AAAAGCGCTGTAAATTGAATCCTGAACTAAAAATCTATAATCAAGAAATCAACAAAAGAAGAATTGGGATTGAGCATGTATTTGGCAGTTTAAAAACCTT
CAAAATCCTTGCTGAGCGATATCGTAATCGAGGAAAAAGACTGGGTTTAAGATTCAATTTAATTGCTGGAATCTACAATATGGAACTGAGTAAAAAATGA
CTTATGAAATAAGTCT
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 411 bp | 136 aa | 72 | 482 | + | No |
Chemistry : DDE
ORF sequence :
MHNLRMKYIDSKKLSETQFKRYTGISWSTFDLMVEQLKMHVPAKGRPTKLSIEDQILLCLSYWREYRTLFHVATSYGVSEPTASRIVRHVEDCLIKSNLF
NLPKHLPKGEGIDWNVVIVDATEIPIQRPKKTEEKL
NLPKHLPKGEGIDWNVVIVDATEIPIQRPKKTEEKL
Blast result :Comments : First part of transposase.ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 477 bp | 158 aa | 424 | 900 | + | No |
Chemistry : DDE
ORF sequence :
LLMPQKFQYKDLKKQKKSYSGKKKAHTFKVQAIIHYRTQQILSLCTSRGAVHDFALFKRNLHQIPAGAFILADKGYQGIYSVYPNSLLPLKAKKRCKLNP
ELKIYNQEINKRRIGIEHVFGSLKTFKILAERYRNRGKRLGLRFNLIAGIYNMELSKK
ELKIYNQEINKRRIGIEHVFGSLKTFKILAERYRNRGKRLGLRFNLIAGIYNMELSKK
Blast result :Comments : Second part of transposase.ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 829 bp | 275 aa | 72 | 900 | + | Yes |
Chemistry : DDE
ORF sequence :
MHNLRMKYIDSKKLSETQFKRYTGISWSTFDLMVEQLKMHVPAKGRPTKLSIEDQILLCLSYWREYRTLFHVATSYGVSEPTASRIVRHVEDCLIKSNLF
NLPKHLPKGEGIDWNVVIVDATEIPIQRPKKQKKSYSGKKKAHTFKVQAIIHYRTQQILSLCTSRGAVHDFALFKRNLHQIPAGAFILADKGYQGIYSVY
PNSLLPLKAKKRCKLNPELKIYNQEINKRRIGIEHVFGSLKTFKILAERYRNRGKRLGLRFNLIAGIYNMELSKK
NLPKHLPKGEGIDWNVVIVDATEIPIQRPKKQKKSYSGKKKAHTFKVQAIIHYRTQQILSLCTSRGAVHDFALFKRNLHQIPAGAFILADKGYQGIYSVY
PNSLLPLKAKKRCKLNPELKIYNQEINKRRIGIEHVFGSLKTFKILAERYRNRGKRLGLRFNLIAGIYNMELSKK
Blast result :
Comments
ISAlw32 is 83% similar to ISAlw14.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
A. lwoffii NCTC5866 carries a single copy of ISAlw32.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
A. lwoffii NCTC5866 carries a single copy of ISAlw32.
References