ISAlw15
- Family IS5
- Group ISL2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Acinetobacter lwoffii | Acinetobacter lwoffii Acinetobacter lwoffii M2a |
DNA section
IS Length : 951 bp
Ends
IR Length : 20
IRL : TAGACTTCCGTCATAAATCAAAAAACGTACAATTTATTTGATCTTAAATT
IRR : TAGACTTCCTTCATAAATCATTTTTCAATCAATTTAATTGTTTATCTTTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| gtctatttaggcagcattat | ttgattggggaatatgaagc | 0 | |
| ttttatatctcgtgattcag | gtgaatatgaggtttattga | 0 | |
| ctatgtgggtgctaaaacag | gtatcaataatacctctgat | 0 | |
| tgtattgcatttatattcaa | atgtttaaggttgatataaa | 0 | |
| agtcattttatttttatcaa | atgacagcaagttttcttaa | 0 | |
| ttaaagtcgtcaaaagctat | ttgaaagatggttatacaat | 0 |
DNA sequence
TAGACTTCCGTCATAAATCAAAAAACGTACAATTTATTTGATCTTAAATTAATCAGCAAAATTATTTAATTCAAGAGATAATCCTTGGATGAAATTCAAA
GATATTCAAAAATTATCAAACGTTAAGTTTCGTAGGCTTACCGGTGTTAGTTGGGCTACATTTAACCTCATGTTGGCCGAGCTAAATAAGCATTTACCTC
GTCATATTGGTAAAGGACGACCGCATAAATTACCGTTGGAAGATCGTTTGCTCTTATGTATAGAATATTGGAGAGAATATCGAACATTTTTCCATCTTGG
TATGAGTTACGGTGTATCTGAAACGAGTGCAATTCGTATCACACGTGTTATTGAAGATACCTTAATCCGTTCTGGAAAATTTAACTTGCCAAAACAACTT
CCTAATCGAGATGAAGTGGATTGGGAAGTTGTTGTGATTGATGCAACTGAAATATTAGTTCAACGTCCAAAAAAAACAGAAGAAATGGTATAGCGGTAAA
AAAAAGCGACATACCTTTAAATTTCAGCTATCTATGCACTATACAACAGGTGAAATACTGAGTGTATGTGGAAGTCATGGAAGCATGCATGATTTTAAAA
TATTTAAAAAAAGTATGAGGAAATTAAAATTTAAGCCCTTTTTTATCGTTGATAAGGGGTATTTAGGGATAAAAAAATTGGGTTTTGGATACCTCATGCC
ATCTAAAGCAAAGAAAACTGAAAAATTAGATTCTGAATTAAAAAAGTTAAATACAGAGATTGGTCGTAGACGAATTCAAGTAGAGCATGTATTTGGAAGG
ATGAAATGTTTTAAGATTTTATCTTGTGTATATAGAAATCGTCGTAAAAGATTAAACCTGCGGTTTAATTTACTTGCTGGCATATACAATTTAGATTGGG
TGAAAGATAAACAATTAAATTGATTGAAAAATGATTTATGAAGGAAGTCTA
GATATTCAAAAATTATCAAACGTTAAGTTTCGTAGGCTTACCGGTGTTAGTTGGGCTACATTTAACCTCATGTTGGCCGAGCTAAATAAGCATTTACCTC
GTCATATTGGTAAAGGACGACCGCATAAATTACCGTTGGAAGATCGTTTGCTCTTATGTATAGAATATTGGAGAGAATATCGAACATTTTTCCATCTTGG
TATGAGTTACGGTGTATCTGAAACGAGTGCAATTCGTATCACACGTGTTATTGAAGATACCTTAATCCGTTCTGGAAAATTTAACTTGCCAAAACAACTT
CCTAATCGAGATGAAGTGGATTGGGAAGTTGTTGTGATTGATGCAACTGAAATATTAGTTCAACGTCCAAAAAAAACAGAAGAAATGGTATAGCGGTAAA
AAAAAGCGACATACCTTTAAATTTCAGCTATCTATGCACTATACAACAGGTGAAATACTGAGTGTATGTGGAAGTCATGGAAGCATGCATGATTTTAAAA
TATTTAAAAAAAGTATGAGGAAATTAAAATTTAAGCCCTTTTTTATCGTTGATAAGGGGTATTTAGGGATAAAAAAATTGGGTTTTGGATACCTCATGCC
ATCTAAAGCAAAGAAAACTGAAAAATTAGATTCTGAATTAAAAAAGTTAAATACAGAGATTGGTCGTAGACGAATTCAAGTAGAGCATGTATTTGGAAGG
ATGAAATGTTTTAAGATTTTATCTTGTGTATATAGAAATCGTCGTAAAAGATTAAACCTGCGGTTTAATTTACTTGCTGGCATATACAATTTAGATTGGG
TGAAAGATAAACAATTAAATTGATTGAAAAATGATTTATGAAGGAAGTCTA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 405 bp | 134 aa | 89 | 493 | + | No |
Description : First part of the transposase
ORF sequence :
MKFKDIQKLSNVKFRRLTGVSWATFNLMLAELNKHLPRHIGKGRPHKLPLEDRLLLCIEYWREYRTFFHLGMSYGVSETSAIRITRVIEDTLIRSGKFNL
PKQLPNRDEVDWEVVVIDATEILVQRPKKTEEMV
PKQLPNRDEVDWEVVVIDATEILVQRPKKTEEMV
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 465 bp | 154 aa | 459 | 923 | + | No |
Description : Second part of the transposase
ORF sequence :
FNVQKKQKKWYSGKKKRHTFKFQLSMHYTTGEILSVCGSHGSMHDFKIFKKSMRKLKFKPFFIVDKGYLGIKKLGFGYLMPSKAKKTEKLDSELKKLNTE
IGRRRIQVEHVFGRMKCFKILSCVYRNRRKRLNLRFNLLAGIYNLDWVKDKQLN
IGRRRIQVEHVFGRMKCFKILSCVYRNRRKRLNLRFNLLAGIYNLDWVKDKQLN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 835 bp | 277 aa | 89 | 923 | + | Yes |
Chemistry : DDE
ORF sequence :
MKFKDIQKLSNVKFRRLTGVSWATFNLMLAELNKHLPRHIGKGRPHKLPLEDRLLLCIEYWREYRTFFHLGMSYGVSETSAIRITRVIEDTLIRSGKFNL
PKQLPNRDEVDWEVVVIDATEILVQRPKKQKKWYSGKKKRHTFKFQLSMHYTTGEILSVCGSHGSMHDFKIFKKSMRKLKFKPFFIVDKGYLGIKKLGFG
YLMPSKAKKTEKLDSELKKLNTEIGRRRIQVEHVFGRMKCFKILSCVYRNRRKRLNLRFNLLAGIYNLDWVKDKQLN
PKQLPNRDEVDWEVVVIDATEILVQRPKKQKKWYSGKKKRHTFKFQLSMHYTTGEILSVCGSHGSMHDFKIFKKSMRKLKFKPFFIVDKGYLGIKKLGFG
YLMPSKAKKTEKLDSELKKLNTEIGRRRIQVEHVFGRMKCFKILSCVYRNRRKRLNLRFNLLAGIYNLDWVKDKQLN
Blast result :
Comments
ISAlw15 is 63% aa similar to ISCaa3.
The third ORF is the putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
Five slightly different variants occur among the 6 ISAlw15 copies of A. lwoffii M2a (2, 3, 5, 9 SNPs compared to the prototype). The prototype was defined according to the only copy, which was fully assembled by A5-Miseq assembly pipeline.
The third ORF is the putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
Five slightly different variants occur among the 6 ISAlw15 copies of A. lwoffii M2a (2, 3, 5, 9 SNPs compared to the prototype). The prototype was defined according to the only copy, which was fully assembled by A5-Miseq assembly pipeline.
References