ISAlw14
- Family IS5
- Group ISL2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Acinetobacter lwoffii | Acinetobacter lwoffii Acinetobacter lwoffii M2a |
DNA section
IS Length : 916 bp
Ends
IR Length : 19
IRL : AGATCTCTTTCATAAATCAAAAAACGATCTTCTTTTTGGTTAATATTTGC
IRR : AGACCTCTTTCATAAATCATTTTTTGCTCAGTTCCAAGTTGTAGATTCCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| tttttattttaattttgata | agt | gttatattaattttggtttg | 3 |
DNA sequence
AGATCTCTTTCATAAATCAAAAAACGATCTTCTTTTTGGTTAATATTTGCACTCCGGATTTCTTGGCATTCGTGCATAATCTGCGGATGAAATACATCGA
TTCAAACAAGCTTTCTGATCCTCAGTTCAAGCGATACACAGGCATCTCATGGTCAACTTTCTATTTGATGGTTGAGCAATTGAAGAAACATGTCCCTGCC
AAAGGCAGACCGCCCAAGTTAAGCCTGGAAGACCAGGTTCTGCTCTGTCTGAGCTATTGGCGGGAATACCGAACCTTATTTCACGTTGCGACGAGCTACG
GGGTTTCAGAGCCCACAGCCTCAAGAATTGTCCGTCATGTTGAAGACTGCCTGATTCGGTCCAATCTCTTTAATTTACCCAAAGATTTACCCGAGGGCGA
AGGCATCGATTGGAATGTTGTGATCGTGGATGCCACAGAAATTCCAATCCAAAGGCCTAAAAAAACAGAAGAAAAGCTATAGCGGCAAGAAAAAGACCCA
CACTTTCAAAGTACAGGCCATGATTCACTACAAAACTCAGCAAATTCTGAGTTTATGTACCAGTCGCGGTGCAGTGCATGATTTCGAGTTGTTCAAACGC
AATTTAAACCAGATTCCTTTTGGGGCTTTTATCCTTGCAGATAAAGGCTATCAGGGGATTTATGTGTTGTATCCGAATAGCCTGTTGCCATTAAAAGCCA
AAAGACACTGTAAATTGGATCCTGAATTGAAAATCTATAATCAAGAAATCAATAAAAGAAGAATCGGAATTGAGCATGTATTTGGCAGCCTGAAAACCTT
CAAAATCCTTGCTGAGCGTTATCGAAATAGAGGTAAAAGGCTTGGTTTGAGATTCAATTTAATCGCTGGAATCTACAACTTGGAACTGAGCAAAAAATGA
TTTATGAAAGAGGTCT
TTCAAACAAGCTTTCTGATCCTCAGTTCAAGCGATACACAGGCATCTCATGGTCAACTTTCTATTTGATGGTTGAGCAATTGAAGAAACATGTCCCTGCC
AAAGGCAGACCGCCCAAGTTAAGCCTGGAAGACCAGGTTCTGCTCTGTCTGAGCTATTGGCGGGAATACCGAACCTTATTTCACGTTGCGACGAGCTACG
GGGTTTCAGAGCCCACAGCCTCAAGAATTGTCCGTCATGTTGAAGACTGCCTGATTCGGTCCAATCTCTTTAATTTACCCAAAGATTTACCCGAGGGCGA
AGGCATCGATTGGAATGTTGTGATCGTGGATGCCACAGAAATTCCAATCCAAAGGCCTAAAAAAACAGAAGAAAAGCTATAGCGGCAAGAAAAAGACCCA
CACTTTCAAAGTACAGGCCATGATTCACTACAAAACTCAGCAAATTCTGAGTTTATGTACCAGTCGCGGTGCAGTGCATGATTTCGAGTTGTTCAAACGC
AATTTAAACCAGATTCCTTTTGGGGCTTTTATCCTTGCAGATAAAGGCTATCAGGGGATTTATGTGTTGTATCCGAATAGCCTGTTGCCATTAAAAGCCA
AAAGACACTGTAAATTGGATCCTGAATTGAAAATCTATAATCAAGAAATCAATAAAAGAAGAATCGGAATTGAGCATGTATTTGGCAGCCTGAAAACCTT
CAAAATCCTTGCTGAGCGTTATCGAAATAGAGGTAAAAGGCTTGGTTTGAGATTCAATTTAATCGCTGGAATCTACAACTTGGAACTGAGCAAAAAATGA
TTTATGAAAGAGGTCT
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 396 bp | 131 aa | 87 | 482 | + | No |
Description : First part of the transposase
ORF sequence :
MKYIDSNKLSDPQFKRYTGISWSTFYLMVEQLKKHVPAKGRPPKLSLEDQVLLCLSYWREYRTLFHVATSYGVSEPTASRIVRHVEDCLIRSNLFNLPKD
LPEGEGIDWNVVIVDATEIPIQRPKKTEEKL
LPEGEGIDWNVVIVDATEIPIQRPKKTEEKL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 477 bp | 158 aa | 424 | 900 | + | No |
Description : Second part of the transposase
ORF sequence :
SWMPQKFQSKGLKKQKKSYSGKKKTHTFKVQAMIHYKTQQILSLCTSRGAVHDFELFKRNLNQIPFGAFILADKGYQGIYVLYPNSLLPLKAKRHCKLDP
ELKIYNQEINKRRIGIEHVFGSLKTFKILAERYRNRGKRLGLRFNLIAGIYNLELSKK
ELKIYNQEINKRRIGIEHVFGSLKTFKILAERYRNRGKRLGLRFNLIAGIYNLELSKK
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 814 bp | 270 aa | 87 | 900 | + | Yes |
Chemistry : DDE
ORF sequence :
MKYIDSNKLSDPQFKRYTGISWSTFYLMVEQLKKHVPAKGRPPKLSLEDQVLLCLSYWREYRTLFHVATSYGVSEPTASRIVRHVEDCLIRSNLFNLPKD
LPEGEGIDWNVVIVDATEIPIQRPKKQKKSYSGKKKTHTFKVQAMIHYKTQQILSLCTSRGAVHDFELFKRNLNQIPFGAFILADKGYQGIYVLYPNSLL
PLKAKRHCKLDPELKIYNQEINKRRIGIEHVFGSLKTFKILAERYRNRGKRLGLRFNLIAGIYNLELSKK
LPEGEGIDWNVVIVDATEIPIQRPKKQKKSYSGKKKTHTFKVQAMIHYKTQQILSLCTSRGAVHDFELFKRNLNQIPFGAFILADKGYQGIYVLYPNSLL
PLKAKRHCKLDPELKIYNQEINKRRIGIEHVFGSLKTFKILAERYRNRGKRLGLRFNLIAGIYNLELSKK
Blast result :Comments : The third ORF is a putative ORFAB transposase reconstructed in silico by a possible +1 frameshift.
Comments
ISAlw14 is 62% aa similar to ISCaa3.
The third ORF is the putative ORFAB transposase reconstructed in silico by a possible +1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by a possible +1 frameshift.
References