ISAba49

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP026749 ND Acinetobacter baumannii
Acinetobacter baumannii WCHAB005133 plasmid pOXA58_005133
DNA section
IS Length : 2554 bp

Ends


IR Length : 16

IRL : GTAAGCGTCCGCTGAACACACCTTATGAATTCATCCTATAAGCCTTAATT
IRR : GTAAGCGTCCGCTGAATCCCATACCTATTTTTTAATTTTGGTCGGATTTC

Insertion site


Left flankDirect repeatRight flankDR Length
AGCAAGCTCTTTTTGAGAAATTTCGAGTTCTTCACGAATAAGAT8

DNA sequence

GTAAGCGTCCGCTGAACACACCTTATGAATTCATCCTATAAGCCTTAATTTAGTCCCCACTTAAAAACAAGTGGGGACTAAAATTTATGACTACAAATCA
CCAGACATCCATCGCATCTCTTGCGAAAAAACGAAGAACATACAGTGCTGAATTTAAACAGCAGATCGTTCAGGCTTGTAAAGCACCGGACGTTTCAATT
GCTTCAGTCGCTTTGCAACATGGATTGAATACAAATCTTGTATCCAAATGGATTCGCTTAATTGATGCTAAGCCAGGGAATGATCGCTCACCACTACCGA
ATAAACCTGCATTTATTGCCTTATCTTGCTCTGCACCATTAGATCCTACTCCTACTGACATGTTAACGGTTCAAATTACTTTACCCCACTCAAAAGCAGA
AATTGGCTTGAAATGGCAAGTATCAGAAATATCTGCTTTAGCAGAATTACTCAAGGCACTTGCAACATGATCCGCATTGATGAAATCTGGCTTTCTACCC
AACCTCTGGATATGCGAGCAGGGATGGATACGGTCATGGCGCAGATTATGAAAGCCTTTGGCTACATTAAACCGCATTGCGCCTACCTGTTCTGTAATAA
ACGTGGCCATCGCATGAAAGTGCTGGTACATGATGGACTGGGCATCTGGCTGTGTGCCCGGCGGTTGGAACAGGGCAAATTTCACTGGGCTAAAGTTCAC
CAGGGTGAAAGCGTAGCCCTCAGCCCGGAACAGTTACAGGCACTGATCCAGGGTTTGCCTTGGCAGCGCATTGGACGACAGCAAGTGGTCACAATGCTCT
AAACTAGCTTGGTCCATTCTGCTATTCTCCAAATGTTGTATTTCATTCTTCTCATGACCTCAGGCATACTGCGGTCATGAATACGCTGCCTGATTTAAGC
CAACTGACCCATGAACAACTGCTGGAATTTACCAGACAGTTGGCAATGCAGCATCAGTCTCTGGCACAATCAAATCAAGAATTAGAAAAATCAAACCAAC
AATTAGATACCAAAGTTCAGCATCTTGAAGTATCCAATCAGCAATTAGATGCTCAAGTTCAACATCTTTCCATTCTCAATCAAAAATACGAGCATGAACT
TGCGCTGTTTAAAAAGCACAAATTCGGCAGTAAAAACGAACATCTCACTGCAAAACAAATCCACCTGTGGGATGAAGCGGTTGAAGAAGATATTGCCGCG
GTTGATCTAGAACTGGAACGGCTGAATGCAGATAAAACCGATGCAGTGACACACAAAGCCAAAACCAATAAACCTAAACGTCGACCACTGCCAGATCATC
TACACACCATCCGTATTGAGCATGAACCTGCATCAACCCAATGTGCTTGTGGCTGCCAACTTCGTCGTATCGGCGAAGATGTCAGTGAAAAACTGCATTT
CAGACCGGCACAGTTCTATAAGGAACAGCATGTGCGTGGTAAATGGGTCTGTGATCAGTGTGACACCCTGACTCAGCAAGCGATGCCCGCCTATGTGATT
GATAAAGGCATTGCTTCACCTGAACTGCTCAGCCATGTTTTGGTATCGAAATATGCCGATCATTTGCCGCTGTACCGTCAACGTCTGATCTATCAGCGGG
CGGGAATCGAACTTTCTAGATCAACTTTATCTGACTGGATAGGTCGCTGCGGTGTAGAACTGGAACCTCTGGCCAATGCCTTAAAAGAGGTGGTGCTGCA
ACAGCAGGTGCTGCATGCAGATGAAACACCAGTCACCATTATGCGGATGGGTGAGAATGAGAAAAAACCGAAAAAAGGTTATGTCTGGGCCTATGCCACT
ACACAGTACAATCCAGTTCAGGCGGTGATCTATGACTTTCAGGATAGTCGTTCAGGCCAGCATGCTGCAGAGTTCTTGAAAGGCTGGCAGGGCCATCTGG
TCTGTGATGATTACAGTGGTTATAAAGCACGCTTTAAATCAGGCCAGGTCATAGAGGTGGGCTGCATGGCCCATGCACGTCGTAAATTCCATGAACTGCA
TGTGACCGGGAAAAGTCAGGTCGCTGAACAGGCCTTAGTGCTGATTCAGAAACTGTATGCGATAGAAGCAGAACTCAGGAAAAAGACCGATGGTACAGCG
GAAGACCGCCGCGAATACCGACAACAGCATAGTCAACCGGTGATGCAACAACTGTATGAATGGCTTAACCAACATCAACTGACTGTGCCATCGAGTTCTC
CAACCGCCAAGGCCATCAATTACACTCTGAAGCGTTGGCCAGCTTTAAGCCGCTATCTGGATGATGGCAATCTACCTATAGATAATAATTGGGTGGAAAA
CCAGATGCGACCTTGGGCGTTGGGACGTAAGAATTGGCTATTTGCAGGTTCGCTGCGCAGCGGTCAGCGAGCAGCCAATATCATGACATTAATCCAGTCA
GCAAAGCTGAATGGGCTGGATCCGTATGCCTATTTAAGTGATGTTCTGAAAAGGTTGCCGACACATAAAGTGACCCAAATAGAAGAATTACTGCCACACC
GCTGGAAATCCGACCAAAATTAAAAAATAGGTATGGGATTCAGCGGACGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
384 bp127 aa87470+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MTTNHQTSIASLAKKRRTYSAEFKQQIVQACKAPDVSIASVALQHGLNTNLVSKWIRLIDAKPGNDRSPLPNKPAFIALSCSAPLDPTPTDMLTVQITLP
HSKAEIGLKWQVSEISALAELLKALAT

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
390 bp129 aa413802+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MASIRNICFSRITQGTCNMIRIDEIWLSTQPLDMRAGMDTVMAQIMKAFGYIKPHCAYLFCNKRGHRMKVLVHDGLGIWLCARRLEQGKFHWAKVHQGES
VALSPEQLQALIQGLPWQRIGRQQVVTML

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1647 bp548 aa8772523+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNTLPDLSQLTHEQLLEFTRQLAMQHQSLAQSNQELEKSNQQLDTKVQHLEVSNQQLDAQVQHLSILNQKYEHELALFKKHKFGSKNEHLTAKQIHLWDE
AVEEDIAAVDLELERLNADKTDAVTHKAKTNKPKRRPLPDHLHTIRIEHEPASTQCACGCQLRRIGEDVSEKLHFRPAQFYKEQHVRGKWVCDQCDTLTQ
QAMPAYVIDKGIASPELLSHVLVSKYADHLPLYRQRLIYQRAGIELSRSTLSDWIGRCGVELEPLANALKEVVLQQQVLHADETPVTIMRMGENEKKPKK
GYVWAYATTQYNPVQAVIYDFQDSRSGQHAAEFLKGWQGHLVCDDYSGYKARFKSGQVIEVGCMAHARRKFHELHVTGKSQVAEQALVLIQKLYAIEAEL
RKKTDGTAEDRREYRQQHSQPVMQQLYEWLNQHQLTVPSSSPTAKAINYTLKRWPALSRYLDDGNLPIDNNWVENQMRPWALGRKNWLFAGSLRSGQRAA
NIMTLIQSAKLNGLDPYAYLSDVLKRLPTHKVTQIEELLPHRWKSDQN

 

Blast result :
Comments
ISAba49 is 98% aa similar to ISAba16.
References
1] ISfinder submission (2019)
2] Feng,Y., McNally,A. and Wang,X. (2018) Direct GenBank submission.