ISEnfa200
- Family IS200/IS605
- Group IS200
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF125554 | ND | Enterococcus faecium | Enterococcus faecium TUH12-5 Enterococcus faecium TUH7-15 Enterococcus faecium TUH12-1 Enterococcus faecium TUH12-3 Enterococcus faecium TUH12-4 |
DNA section
IS Length : 787 bp
Ends
Left end : TTAGGCCAAGGGCCTGTTTTCTGGCCCGGAGTTTTTCGGGTTCCGAAAAACGGAGGGTTAGAAAACCAAAAAACCACTTGCTATGCAAGTGGATAAGGAT II struct. : No
Right end : TGCGATTGGCGAACCAAATCGATGAGTCTTTAGACTCCAGTGCCGGTAAAAGACCCTTATAGGGTCAAAGCAAGCCACCGGCTAAGCCGGTGGTCTTGAC II struct. : No
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| GGTCTTTTTT | CTGCTACAGG |
DNA sequence
TTAGGCCAAGGGCCTGTTTTCTGGCCCGGAGTTTTTCGGGTTCCGAAAAACGGAGGGTTAGAAAACCAAAAAACCACTTGCTATGCAAGTGGATAAGGAT
GCTTCAGCTTACAGAAAAACCTCCAATGTTATAATTAATGCAGGTTCGCCAACCGCATTAAAAACAAAGGAGGTATCCATATGGATAGTAATAGTCTATC
ACATACCAGATGGAATTGTAAGTATCATATAGTTTTTGCACCGAAGTATAGAAGAAAAGTAGCATATGGAAAAATAAAACAGGATATAGCCAATATCCTA
AGTATGTTATGCAAGAGGAAAGGCGTAAAAATTATAGAAGCAGAGATATGTCCAGATCACGTCCATATGCTGGTAGAAATTCCGCCAAGCATGAGTGTAT
CGTATTTTGTAGGGTATCTGAAAGGGAAAAGTACACTAATGATATTCGAGCGGCATGCCAATTTGAAATATAAATATGGGAACCGACATTTCTGGTGCAG
AGGATACTACGTAGATACGGTAGGGAAAAATGCGAAGAAAATAGAGGAATATATAGCCAATCAGCTACAGGAAGATCTGGAATATGATCAAATGACACTG
AAAGAGTATATAGACCCGTTCACGGGTGAGCCAGTAAAGCAAAACAGATAAGAAGAGCCCTTTAGGGCTCAAGTAAGAAAATGATGTTGCGATTGGCGAA
CCAAATCGATGAGTCTTTAGACTCCAGTGCCGGTAAAAGACCCTTATAGGGTCAAAGCAAGCCACCGGCTAAGCCGGTGGTCTTGAC
GCTTCAGCTTACAGAAAAACCTCCAATGTTATAATTAATGCAGGTTCGCCAACCGCATTAAAAACAAAGGAGGTATCCATATGGATAGTAATAGTCTATC
ACATACCAGATGGAATTGTAAGTATCATATAGTTTTTGCACCGAAGTATAGAAGAAAAGTAGCATATGGAAAAATAAAACAGGATATAGCCAATATCCTA
AGTATGTTATGCAAGAGGAAAGGCGTAAAAATTATAGAAGCAGAGATATGTCCAGATCACGTCCATATGCTGGTAGAAATTCCGCCAAGCATGAGTGTAT
CGTATTTTGTAGGGTATCTGAAAGGGAAAAGTACACTAATGATATTCGAGCGGCATGCCAATTTGAAATATAAATATGGGAACCGACATTTCTGGTGCAG
AGGATACTACGTAGATACGGTAGGGAAAAATGCGAAGAAAATAGAGGAATATATAGCCAATCAGCTACAGGAAGATCTGGAATATGATCAAATGACACTG
AAAGAGTATATAGACCCGTTCACGGGTGAGCCAGTAAAGCAAAACAGATAAGAAGAGCCCTTTAGGGCTCAAGTAAGAAAATGATGTTGCGATTGGCGAA
CCAAATCGATGAGTCTTTAGACTCCAGTGCCGGTAAAAGACCCTTATAGGGTCAAAGCAAGCCACCGGCTAAGCCGGTGGTCTTGAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 471 bp | 156 aa | 181 | 651 | + | No |
Chemistry : Y1
ORF sequence :
MDSNSLSHTRWNCKYHIVFAPKYRRKVAYGKIKQDIANILSMLCKRKGVKIIEAEICPDHVHMLVEIPPSMSVSYFVGYLKGKSTLMIFERHANLKYKYG
NRHFWCRGYYVDTVGKNAKKIEEYIANQLQEDLEYDQMTLKEYIDPFTGEPVKQNR
NRHFWCRGYYVDTVGKNAKKIEEYIANQLQEDLEYDQMTLKEYIDPFTGEPVKQNR
Blast result :
Comments
Occurence: Only 5 of 197 Enterococci screened were positive for this IS element. Typing by PFGE showed possible relatedness between the 5 hosts. These 5 strains had one copy of the IS element which were located at the exact same target site in the intergenic region between vanS and vanY in VanB-type vancomycin resistance gene cluster.
References