ISEhe5
- Family IS1
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF327446 | ND | Pantoea agglomerans | Pantoea agglomerans plasmid pPATH |
DNA section
IS Length : 785 bp
Ends
IR Length : 26/33
IRL : GGTCAAGTATTCAAAGCGTTGATCATGTTATAAAATCACTCTTTTTACAG
IRR : GGTCACGTATTCAATATGTAGATCCGGTTATAATACGTAATACTCACGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GGTCAAGTATTCAAAGCGTTGATCATGTTATAAAATCACTCTTTTTACAGAGTACATCTGATGGCTAAAGTTGACGTAAAATGCCCTTTTTGTGAGCAGA
CCGAATCCGTTAAAAAACATGGGCAGGGTAAAACCGGCCATCAGCGCTATCGCTGCCAGTTATGCCGCCGTACTTTCCAGCTCGACTATGCCTATCGTGC
CTGTCAGCCCGGAATGAAAGAACAGATTGTCGATCTTGCAATGAACAATGCGTGCATTCGTTACACCGCCCGAGCGCTACATATCAGTATCAATGCCGTT
GTTCGCACCTTAAAAAACTCTCGCCGCGATGTGTAACCACACTCCCACTGGATAATATCCAGATTCAGCTTATCTGCGAAGTTGATGAAATGTGGTCATT
TGTTGGCAATAAGAAGCAACAGCGCTGGCTGTTATATGCATGGGAGCCTCGTCTCAAACGCATTATTGCCCATGCTTTCGGGGGTCGGAGTAAAAAGAGG
CTGCGTAAATTACTGGGGTTACTGTCGGGCTTCAATATTGTCTTCTGGTGTTCAGATAACTTCAGCGCTTATGAACTGTTGCCGGGTAAAAATCACATCA
CAGGCAAGCTTTACACGCAGCGCGTTGAGCGAGAAAACCTGAATCTTCGTAACCGGTTAAAACGACTCAATCGCAAGACTCTTGGGTACTCAAAATCAGC
AGAAATGCACGATAAGGTAATCGGTACGTTCATCGAGCGTGAGTATTACGTATTATAACCGGATCTACATATTGAATACGTGACC
CCGAATCCGTTAAAAAACATGGGCAGGGTAAAACCGGCCATCAGCGCTATCGCTGCCAGTTATGCCGCCGTACTTTCCAGCTCGACTATGCCTATCGTGC
CTGTCAGCCCGGAATGAAAGAACAGATTGTCGATCTTGCAATGAACAATGCGTGCATTCGTTACACCGCCCGAGCGCTACATATCAGTATCAATGCCGTT
GTTCGCACCTTAAAAAACTCTCGCCGCGATGTGTAACCACACTCCCACTGGATAATATCCAGATTCAGCTTATCTGCGAAGTTGATGAAATGTGGTCATT
TGTTGGCAATAAGAAGCAACAGCGCTGGCTGTTATATGCATGGGAGCCTCGTCTCAAACGCATTATTGCCCATGCTTTCGGGGGTCGGAGTAAAAAGAGG
CTGCGTAAATTACTGGGGTTACTGTCGGGCTTCAATATTGTCTTCTGGTGTTCAGATAACTTCAGCGCTTATGAACTGTTGCCGGGTAAAAATCACATCA
CAGGCAAGCTTTACACGCAGCGCGTTGAGCGAGAAAACCTGAATCTTCGTAACCGGTTAAAACGACTCAATCGCAAGACTCTTGGGTACTCAAAATCAGC
AGAAATGCACGATAAGGTAATCGGTACGTTCATCGAGCGTGAGTATTACGTATTATAACCGGATCTACATATTGAATACGTGACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 276 bp | 91 aa | 61 | 336 | + | No |
Description : First part of the transposase
ORF sequence :
MAKVDVKCPFCEQTESVKKHGQGKTGHQRYRCQLCRRTFQLDYAYRACQPGMKEQIVDLAMNNACIRYTARALHISINAVVRTLKNSRRDV
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 627 bp | 208 aa | 132 | 758 | + | No |
Description : Second part of the transposase
ORF sequence :
NRPSALSLPVMPPYFPARLCLSCLSARNERTDCRSCNEQCVHSLHRPSATYQYQCRCSHLKKLSPRCVTTLPLDNIQIQLICEVDEMWSFVGNKKQQRWL
LYAWEPRLKRIIAHAFGGRSKKRLRKLLGLLSGFNIVFWCSDNFSAYELLPGKNHITGKLYTQRVERENLNLRNRLKRLNRKTLGYSKSAEMHDKVIGTF
IEREYYVL
LYAWEPRLKRIIAHAFGGRSKKRLRKLLGLLSGFNIVFWCSDNFSAYELLPGKNHITGKLYTQRVERENLNLRNRLKRLNRKTLGYSKSAEMHDKVIGTF
IEREYYVL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 698 bp | 232 aa | 61 | 758 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKVDVKCPFCEQTESVKKHGQGKTGHQRYRCQLCRRTFQLDYAYRACQPGMKEQIVDLAMNNACIRYTARALHISINAVVRTLKKLSPRCVTTLPLDNI
QIQLICEVDEMWSFVGNKKQQRWLLYAWEPRLKRIIAHAFGGRSKKRLRKLLGLLSGFNIVFWCSDNFSAYELLPGKNHITGKLYTQRVERENLNLRNRL
KRLNRKTLGYSKSAEMHDKVIGTFIEREYYVL
QIQLICEVDEMWSFVGNKKQQRWLLYAWEPRLKRIIAHAFGGRSKKRLRKLLGLLSGFNIVFWCSDNFSAYELLPGKNHITGKLYTQRVERENLNLRNRL
KRLNRKTLGYSKSAEMHDKVIGTFIEREYYVL
Blast result :
Comments
ISEhe5 is 67% and 71% aa similar to respectively ORF1 and ORF2 of IS1N.
pPATH is a plasmid of Erwinia herbicola pvs gypsophilae and betae.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
pPATH is a plasmid of Erwinia herbicola pvs gypsophilae and betae.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Guo,M., Mor,H., Manulis,S. and Barash,I.(2000) Direct submission GenBank