ISArsp13
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| MH067969 | ND | Arthrobacter sp. | Arthrobacter sp. Arthrobacter sp. ANT_H2 pA2H2 |
DNA section
IS Length : 1442 bp
Ends
IR Length : 24/25
IRL : TGAACTGCTCCCCGAAAGTTGGACTGAATAATTCAGATCCTTACTTTCGG
IRR : TGAACTGGTCCCCGAAAGTTGGACTCGGTAAGTAAAAGTCTACGCAGCCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGAACTGCTCCCCGAAAGTTGGACT | TCC | TGAACTGGTCCCCGAAAGTTGGACT | 3 |
DNA sequence
TGAACTGCTCCCCGAAAGTTGGACTGAATAATTCAGATCCTTACTTTCGGGGAGTTTTTCATGCGTCAAAATAGTTCATTGACCGCCGAGCAGCGGCTGG
CTGCCGTCGATCTTTTCGAGCAGGGAATCGGGCATAAGGCGGTATCCTCAAGACTGAACGCCGGTGAACACGCCGTTCGTGGGCTCGAGAAACGGTTCAG
GATTTGGGGTAGGGCAGCGTTGGAAAGCAAACCGACCAATCAGGTGTATTCCTTCGAGTTCAAGCTCGCGGTCGTCCGCCAGTTCCTCGACGGCGAGGCG
ACGCTGATGGAGCTTGCCCAACGACACCGGCTCAGCTCCCCGAACCTTCTCAGGACCTGGGTCAGGACCTACCGGGAACAGGGCGAGGACGGGCTGCGTC
CCAAGGCCAAGGGCCGTCCCAAGACCGCTTCCGGCGCCGAAGCGGGTGGACCCACCGAACTCGAGAAGCTGCGCCGCGAGAACCAGCGGCTGCAGGCGGA
GAATGCATACCTAAAAAAAGTTCGGGCCTTGAGGAACCAACCACCGCGCTGAAAATCAGCGCGGTGATCGCCCTCAAGGCCTCCCACCCCTTGCCCCTGC
TCCTGGCCGCTGCCGGGCTTCCCCGCTCCACGTTCTTCCACCGCCAGGCCGCGCTCAAGGCACCGGACCGGCACGCGGAGCTCCGCGCACGGATCCACGA
GATCTTCACCAAGGCCATGGGCAGGTACGGGCACCGCCGCATCCACGCCGAATTGCTCGGCGGCGGGTGGCAGGTGGCGAAGAAGACCGTGCTGAAGCTG
ATGCGCGCCGAGGGCCTGGTCTGCAAGGTCCGCAGCAAGCGAAGGTATGCCTCCTACAAGGGCACTGTCGGCAAGGTCGCCGAAAACCTGCTGAAGCGCC
AGTTCGACACCGAGGCACCGAACCTGAAGTGGGTGACCGACGTGACCGAATTCAAGATTGCCGACAGGAAGGTCTATCTTTCGCCGGTGCTGGACCTGTT
CGACCGCTCGATCGTTTCCTACTCGGTGTCGCAGTCCCCGACCGTGGCCTTCGCCAACCAATCGCTTAGCGAGGCCATCGGGACCCTGGCCGCGGGCGAG
GCGCCGATGGTGCACTCGGACCAGGGATTCCAGTACCAGCACGCCAGCTGGCAGAGGCTGCTGGCCGACGCCGGCATGACCCAATCCATGTCGCGCAAGG
GCAACTGCCTGGACAACGCCGTGATGGAAAACTTCTTCGGGCACCTGAAAGAAGAGATGTTCCACCACCACGAACACACCAGCGTCGAGGCGTTCACCGC
CGAGCTCGACGACTACATCCACTGGTACAACTATGACCGCATCTCGTTAACGCTCGAGTGCCTGAGCCCGATGAAATACCGGGCCCAGGCACTGGCTGCG
TAGACTTTTACTTACCGAGTCCAACTTTCGGGGACCAGTTCA
CTGCCGTCGATCTTTTCGAGCAGGGAATCGGGCATAAGGCGGTATCCTCAAGACTGAACGCCGGTGAACACGCCGTTCGTGGGCTCGAGAAACGGTTCAG
GATTTGGGGTAGGGCAGCGTTGGAAAGCAAACCGACCAATCAGGTGTATTCCTTCGAGTTCAAGCTCGCGGTCGTCCGCCAGTTCCTCGACGGCGAGGCG
ACGCTGATGGAGCTTGCCCAACGACACCGGCTCAGCTCCCCGAACCTTCTCAGGACCTGGGTCAGGACCTACCGGGAACAGGGCGAGGACGGGCTGCGTC
CCAAGGCCAAGGGCCGTCCCAAGACCGCTTCCGGCGCCGAAGCGGGTGGACCCACCGAACTCGAGAAGCTGCGCCGCGAGAACCAGCGGCTGCAGGCGGA
GAATGCATACCTAAAAAAAGTTCGGGCCTTGAGGAACCAACCACCGCGCTGAAAATCAGCGCGGTGATCGCCCTCAAGGCCTCCCACCCCTTGCCCCTGC
TCCTGGCCGCTGCCGGGCTTCCCCGCTCCACGTTCTTCCACCGCCAGGCCGCGCTCAAGGCACCGGACCGGCACGCGGAGCTCCGCGCACGGATCCACGA
GATCTTCACCAAGGCCATGGGCAGGTACGGGCACCGCCGCATCCACGCCGAATTGCTCGGCGGCGGGTGGCAGGTGGCGAAGAAGACCGTGCTGAAGCTG
ATGCGCGCCGAGGGCCTGGTCTGCAAGGTCCGCAGCAAGCGAAGGTATGCCTCCTACAAGGGCACTGTCGGCAAGGTCGCCGAAAACCTGCTGAAGCGCC
AGTTCGACACCGAGGCACCGAACCTGAAGTGGGTGACCGACGTGACCGAATTCAAGATTGCCGACAGGAAGGTCTATCTTTCGCCGGTGCTGGACCTGTT
CGACCGCTCGATCGTTTCCTACTCGGTGTCGCAGTCCCCGACCGTGGCCTTCGCCAACCAATCGCTTAGCGAGGCCATCGGGACCCTGGCCGCGGGCGAG
GCGCCGATGGTGCACTCGGACCAGGGATTCCAGTACCAGCACGCCAGCTGGCAGAGGCTGCTGGCCGACGCCGGCATGACCCAATCCATGTCGCGCAAGG
GCAACTGCCTGGACAACGCCGTGATGGAAAACTTCTTCGGGCACCTGAAAGAAGAGATGTTCCACCACCACGAACACACCAGCGTCGAGGCGTTCACCGC
CGAGCTCGACGACTACATCCACTGGTACAACTATGACCGCATCTCGTTAACGCTCGAGTGCCTGAGCCCGATGAAATACCGGGCCCAGGCACTGGCTGCG
TAGACTTTTACTTACCGAGTCCAACTTTCGGGGACCAGTTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 492 bp | 163 aa | 61 | 552 | + | No |
Description : First part of the transposase
ORF sequence :
MRQNSSLTAEQRLAAVDLFEQGIGHKAVSSRLNAGEHAVRGLEKRFRIWGRAALESKPTNQVYSFEFKLAVVRQFLDGEATLMELAQRHRLSSPNLLRTW
VRTYREQGEDGLRPKAKGRPKTASGAEAGGPTELEKLRRENQRLQAENAYLKKVRALRNQPPR
VRTYREQGEDGLRPKAKGRPKTASGAEAGGPTELEKLRRENQRLQAENAYLKKVRALRNQPPR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1181 bp | 396 aa | 223 | 1403 | + | No |
Description : Second part of the transposase
ORF sequence :
GSVGKQTDQSGVFLRVQARGRPPVPRRRGDADGACPTTPAQLPEPSQDLGQDLPGTGRGRAASQGQGPSQDRFRRRSGWTHRTREAAPREPAAAGGECIP
KKSSGLEEPTTALKISAVIALKASHPLPLLLAAAGLPRSTFFHRQAALKAPDRHAELRARIHEIFTKAMGRYGHRRIHAELLGGGWQVAKKTVLKLMRAE
GLVCKVRSKRRYASYKGTVGKVAENLLKRQFDTEAPNLKWVTDVTEFKIADRKVYLSPVLDLFDRSIVSYSVSQSPTVAFANQSLSEAIGTLAAGEAPMV
HSDQGFQYQHASWQRLLADAGMTQSMSRKGNCLDNAVMENFFGHLKEEMFHHHEHTSVEAFTAELDDYIHWYNYDRISLTLECLSPMKYRAQALAA
KKSSGLEEPTTALKISAVIALKASHPLPLLLAAAGLPRSTFFHRQAALKAPDRHAELRARIHEIFTKAMGRYGHRRIHAELLGGGWQVAKKTVLKLMRAE
GLVCKVRSKRRYASYKGTVGKVAENLLKRQFDTEAPNLKWVTDVTEFKIADRKVYLSPVLDLFDRSIVSYSVSQSPTVAFANQSLSEAIGTLAAGEAPMV
HSDQGFQYQHASWQRLLADAGMTQSMSRKGNCLDNAVMENFFGHLKEEMFHHHEHTSVEAFTAELDDYIHWYNYDRISLTLECLSPMKYRAQALAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1343 bp | 447 aa | 61 | 1403 | + | Yes |
Chemistry : DDE
ORF sequence :
MRQNSSLTAEQRLAAVDLFEQGIGHKAVSSRLNAGEHAVRGLEKRFRIWGRAALESKPTNQVYSFEFKLAVVRQFLDGEATLMELAQRHRLSSPNLLRTW
VRTYREQGEDGLRPKAKGRPKTASGAEAGGPTELEKLRRENQRLQAENAYLKKSSGLEEPTTALKISAVIALKASHPLPLLLAAAGLPRSTFFHRQAALK
APDRHAELRARIHEIFTKAMGRYGHRRIHAELLGGGWQVAKKTVLKLMRAEGLVCKVRSKRRYASYKGTVGKVAENLLKRQFDTEAPNLKWVTDVTEFKI
ADRKVYLSPVLDLFDRSIVSYSVSQSPTVAFANQSLSEAIGTLAAGEAPMVHSDQGFQYQHASWQRLLADAGMTQSMSRKGNCLDNAVMENFFGHLKEEM
FHHHEHTSVEAFTAELDDYIHWYNYDRISLTLECLSPMKYRAQALAA
VRTYREQGEDGLRPKAKGRPKTASGAEAGGPTELEKLRRENQRLQAENAYLKKSSGLEEPTTALKISAVIALKASHPLPLLLAAAGLPRSTFFHRQAALK
APDRHAELRARIHEIFTKAMGRYGHRRIHAELLGGGWQVAKKTVLKLMRAEGLVCKVRSKRRYASYKGTVGKVAENLLKRQFDTEAPNLKWVTDVTEFKI
ADRKVYLSPVLDLFDRSIVSYSVSQSPTVAFANQSLSEAIGTLAAGEAPMVHSDQGFQYQHASWQRLLADAGMTQSMSRKGNCLDNAVMENFFGHLKEEM
FHHHEHTSVEAFTAELDDYIHWYNYDRISLTLECLSPMKYRAQALAA
Blast result :
Comments
ISArsp13 is 69% aa similar to ISCod1. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Romaniuk, K. (2018) Direct submission.