ISArsp12

  • Family IS481
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
MH067968 ND Arthrobacter sp.
Arthrobacter sp.
Arthrobacter sp. ANT_H2 pA2H1
DNA section
IS Length : 2615 bp

Ends


IR Length : 24/26

IRL : TGTACTGACCGGACACGTTGATCGATAGACAAGTCGCCACTTGTGACAGA
IRR : TGTACTGACCGGACACGTTGGTCAATCGTGAGAAGGGCGGCTGGTTGCTG

Insertion site


Left flankDirect repeatRight flankDR Length
tgtactgaccggacacgttgcctagatgtactgaccggacacgttg6

DNA sequence

TGTACTGACCGGACACGTTGATCGATAGACAAGTCGCCACTTGTGACAGAAGGCCACGCTTGTCTATAGTGTGGGTATGACAGTTTCACTGGATGTTGCC
CTGCCGCGCACGGACCTGTCGGCGCGGGATCACGCGCGGATGCTTGCTCCGTTGTTGAAGCCTTTGTCAGACGAGAACAGACTGATGATCGTGCTGACGC
TTGCGGCGGGGGCGTGCTCGAACAAGAAGTTGCAGGAGGCCACCGGGTTGAGCCAGGCGCTGGTGAGTCACCATGTGGCAGCCTTGCGGCAAGCGCAGCT
GATCAGTGTGCGGGCCGAGGGCCGGTCCAACATCTACGCGCTGTGTTGTGAGCAGTTGGCGGCGCCGGTGCAGTGGCTCGCTCACTTGGCGACTCTCACT
CCAGAGGGACAGAAGGCGTGCTGCACTGCCCCAGCAGGGGAGGGTCAGGCATCGACTGAGGGTGCTGCGCGGTGATTCATGCGGTACAGACCTTCGGGTT
GATCCTGGTCGAGTTGCTCGTCCTGTTCTCGCTGATCTCGGTGCTCGTTGCGCTGGTGAACCGGCGGTTCGGGCCGGATCGGATCCAGAGCTGGATGGCC
GATGGGCGCCTTCCCGGCCCGCTCAAGGGGCTACTGCTGGGGGCGATCACGCCGTTCTGTTCCTGTTCGACGCTGCCGGTGCTGGCTGGGATGTTGAAGT
CGGGGGTGGCGTTCCGGACGTCGATGACCTTCCTGATCTCGTCACCGCTGCTGGATCCCATCATCGTGGCCGGGGTGGTGCTGCTGTTCGACTGGCGTAT
CGCGTTGGTCTACACGGTGGTCACCGCCGCATGGTCCCTGCTCGCCCCCCTAGTATGGGAACGACTCGGCATGGCCAGCCAGCTCAAGCGAGTCAAGGTC
GTCGGCGACGACAGCACCCCCCACCCCTGGGCAGGCCTGCGCAACGAGATCCGGCCCGCACTCGGCGAGGCCTGGGCCGATCTGAGACCGCTGCTCGTCC
CGATGGTGCTGGGTGTCTCGGTCGGTGCATTTATCTACGGTTTCGTGCCTCAGGACCAACTCGCCGCGGTCGCTGGCGACGACAAACCGTGGGCCGTGCC
GTTGGCCGCCGTGCTTGGCGTTCCGCTTTACGTGCGTATCGAGACGATGCTGCCCATCGGCTTGGCCCTCAGCTCGACCGGCATGGGGCTAGGTGCAGTG
TTCGCGCTGATGATCGGCGGCGCCGGAGCCTCGATCCCCGAGATCTCGATGCTCACCACCTTGTTCAAGCCCCGACTGGTCGTCACTTTCGTGGTCACTG
TCATCGGCACGGCCATCGCCGCCGGCTACCTCATCCCACTGATCGCCTGACCCAACCACCACATTCCCAACCACACGAGGAGAACAAGCAACCATGGGAC
TCAAGGACCTCTTCACCGCACGCAAGCAGGACAGCAGTTGCTGCGGGGCCCAGATCGTGCCCGACGATGACGACCAGCCCCAGAGCGAGCAGGGAACACC
CACCGAGACGGCTGCCTCTTCAGCGCCGAACAGCGAGGACACGGACGGCAACTGACATACCGAATCGATGGGGCTGCACCACAGGGTGCAGCCCCATCAC
TGTCTCCACCCGCTCGTCACCAGGAAGACCACCATGTCCCACGCCAATGCCGCCCTCACTCCGCGTCACCGCCTCAAGGTTGCCCAGCTGGTTGTTGACC
ACGGATGGCCGATCAGCGAGGTCGCTGCCCGGTTCCAGGTCTCCTGGCCCACCGTGAAGCGCTGGGCTGACCGCTACCGGGCCGGTCAGTCCATGCAGGA
CCGCAGCTCACGGCCTCACCGCTCCCCGAACAAGACCAGCCCCACGACTGCGAAGCGCTGTATCCAGCTCCGGCTGCGTCTGCGGGAAGGTCCGGTTCAG
TTGGCATACCGGATTGGCGTTGCTCCGTCGACAGTCCACCGGATCTTGGTCGACGTCCACTTGAACCGCCTGTCGCACGTCGACCGTGCCACTGGAGAGC
CTGTTCGTCGCTACGAGCACGACCACCCCGGGGCGATGCTGCACGTCGACGTGAAGAAGCTCGGCAACATCCCAGACGGCGGAGGCTGGCGCTACGTCGG
CCGACGACAAGGCGAGAAGAACCGCGCAGCCACACCCGACAAGCCCAAGAACAAGCACTACGACCCGTTGATGGGCAAGGCCTACGTCCACACCGTCATC
GACGACCACTCCCGCGTCGCCTACGCCGAGATCCACGACGACGAAACAGCCCACACCGCCACAGCGGTCCTGGCCAGGGCCGTCAAGTGGTTCAACGCCC
GCGGGGTGACCGTCGAGCGGGTCCTGTCGGACAACGGTGGCGCCTATCGCTCACACCTGTGGCGCGACACCTGCGCCGAGCTGGGAATCAGGCACAAACG
GACCAGGCCGTATCGCCCGCAGACCAACGGCAAGATCGAGCGCTTCCACCGCACCCTGGCCGACGGCTGGGCCTACGCCCGCTGCTACACCTCCGAGACG
GAGCGTCGCGGCGAACTCGACGGCTGGCTGCACTACTACAACCATCACCGGCCCCACACAGCCTGCAGCAACCAGCCGCCCTTCTCACGATTGACCAACG
TGTCCGGTCAGTACA
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
399 bp132 aa77475+No
ORF function : Passenger Gene
Annotation : ArsR family transcriptional regulatorDescription : Transcriptional Regulator factor

ORF sequence :

MTVSLDVALPRTDLSARDHARMLAPLLKPLSDENRLMIVLTLAAGACSNKKLQEATGLSQALVSHHVAALRQAQLISVRAEGRSNIYALCCEQLAAPVQW
LAHLATLTPEGQKACCTAPAGEGQASTEGAAR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
879 bp292 aa4721350+No
ORF function : Passenger Gene
Annotation : permeaseDescription :

ORF sequence :

VIHAVQTFGLILVELLVLFSLISVLVALVNRRFGPDRIQSWMADGRLPGPLKGLLLGAITPFCSCSTLPVLAGMLKSGVAFRTSMTFLISSPLLDPIIVA
GVVLLFDWRIALVYTVVTAAWSLLAPLVWERLGMASQLKRVKVVGDDSTPHPWAGLRNEIRPALGEAWADLRPLLVPMVLGVSVGAFIYGFVPQDQLAAV
AGDDKPWAVPLAAVLGVPLYVRIETMLPIGLALSSTGMGLGAVFALMIGGAGASIPEISMLTTLFKPRLVVTFVVTVIGTAIAAGYLIPLIA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
987 bp328 aa16342620+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSHANAALTPRHRLKVAQLVVDHGWPISEVAARFQVSWPTVKRWADRYRAGQSMQDRSSRPHRSPNKTSPTTAKRCIQLRLRLREGPVQLAYRIGVAPST
VHRILVDVHLNRLSHVDRATGEPVRRYEHDHPGAMLHVDVKKLGNIPDGGGWRYVGRRQGEKNRAATPDKPKNKHYDPLMGKAYVHTVIDDHSRVAYAEI
HDDETAHTATAVLARAVKWFNARGVTVERVLSDNGGAYRSHLWRDTCAELGIRHKRTRPYRPQTNGKIERFHRTLADGWAYARCYTSETERRGELDGWLH
YYNHHRPHTACSNQPPFSRLTNVSGQYT

 

Blast result :
Comments
ISArsp12 is 92% aa similar to ISPfr21.
References
1] Romaniuk, K. (2018) Direct submission.