ISArsp10

  • Family IS1380
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
MH067976 ND Arthrobacter sp.
Arthrobacter sp.
Arthrobacter sp. ANT_H58 pA58H3
DNA section
IS Length : 1667 bp

Ends


IR Length : 15

IRL : ccttgatccaccgatgagatttgggagctgctcggcgttttaaccacgaa
IRR : ccttgatccaccgatccgcccccggtcgtcgtttgttgacgacggacggg

Insertion site


Left flankDirect repeatRight flankDR Length
GGTGCCGAGACTTAGGGGGCCTGGT5

DNA sequence

CCTTGATCCACCGATGAGATTTGGGAGCTGCTCGGCGTTTTAACCACGAAATGCCCGTCGTATCAGGGAAAATTGAACTTGCGACAGTAACAACATTCCT
GAAATGAAGGGCATCTCGTAGATGCAAGTTTCCCACAAACCCTCCGCCATGTCAGTTTCCTTCGACGAACCGAACCTCGTGTCCACCGCCGGGTTGCTGC
CGGCCATGGTCCTGGCCCGGGACTCAGGCCTGCATGAACTGGCTGACCAATGGCTCAGCGTGCCCACCGACAAAGGTGCCAACCCCGGGTTAAAGATTGC
TTCCCTCGTGGCAGGGATGGTGGCCGGCGCCGACTCCATTGACGATATGGCCCTGTTGCGGCACGGAGCCATGAAAAAGGTCTTCACCGCCTGCTATGCC
CCCTCCACCCTGGGGTCCTTCCTGCGCTCCTTCACCTTCGGGCACGTACGCCAGCTCGATGCCGTGGCCTCCAGGTTCCTGGTGAGCTTGAACGGCAAGG
CCCCACTGCTGGGTGATGTTGCCGCTGATGACTACGTTTACCTCGACGTCGATGACACCATCATCGAGGTCCACGGCTACCAGAAGCAGGGCTCGGGTTA
CGGATATTCTGGGGTGCGCGGGCTCAACGCCTTGCTGGCCACAGCATCCACGAAGGACGCGGTGCCGTTCATTGCCGCCCAACGCCTGCGCAAGGGCGCA
GCCAACTCCGCCCGCGGAGCCAAGAAGTTCGTCACCGAGGCACTGGGCACGCTCACACGTTGCCAACCCGGATCGAAGGTGTTGTGCCGTTTCGACTCGG
CCTACTACGGCCACGGGCCGGTATCGGCGGCTTTAAAGGCGGGCGCGCAGGTCTCGGTGACGGTGCGCATGGACCCGGCCGTGAAGCGGGCGATCGCCAC
GATCCCGGAGGATGCCTGGGAGACCATCCACTACACCGATGCGATCCTTGACGAATCCACCGGGGTCTTGGTCTCCAGCGCCGAGGTCGCCGAAACTTGC
TTCCCCGCGTTCACCTCCCGCAAGAAAGCCGAACGGATTCCCGGGCGCCTAGTGGTGCGCCGGATCCCGGAACTGAACCCGAAGAAGGCCGCCGGGCAAG
GCACCCTCTTTGACACCCACCGTTTCCACGCGTTCTTCACCACCGTGGATTCCGGTGTCCTGGACACTGTCGCTGCAGACAAGACCCACCGCCAACACGC
GGTCATCGAGCAGGTCAACGCGGATTTGAAGGACAGCGCACTGGCCCATATGCCCTCCGGGCACTTCGGAGCCAATAGTGCATGGCTGGTGGCCGCGGCC
ATCGCCTACAACCTCACCCGCGCCGCCGGGATCCTGGCTGGCGGCACCTTCGCCAAGGCTAGGAGCGCAACGGTCCGGCGCAAGCTCATCCAGGTTCCGG
CGCGCATCGCGCGCAGTGCCAGGAAAACCAAGCTCCGGCTTCCGCTGGATTGGCCCTGGGAAACCGGATGGGAACGGCTCTTCACTTCCGTGCACGCCCC
ACCCCAAATCGCCTAGAACCGTTCACCGAGCCCTCTGCGGCGCAACCAGGAAACCATCGTGGACCACCCGCCGGCAGAGAGGCTGGCGCCCTTCCCGTGC
CCAAAAACGAAACCGCCCCCGTCCGTCGTCAACAAACGACGACCGGGGGCGGATCGGTGGATCAAGG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1395 bp464 aa1221516+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MQVSHKPSAMSVSFDEPNLVSTAGLLPAMVLARDSGLHELADQWLSVPTDKGANPGLKIASLVAGMVAGADSIDDMALLRHGAMKKVFTACYAPSTLGSF
LRSFTFGHVRQLDAVASRFLVSLNGKAPLLGDVAADDYVYLDVDDTIIEVHGYQKQGSGYGYSGVRGLNALLATASTKDAVPFIAAQRLRKGAANSARGA
KKFVTEALGTLTRCQPGSKVLCRFDSAYYGHGPVSAALKAGAQVSVTVRMDPAVKRAIATIPEDAWETIHYTDAILDESTGVLVSSAEVAETCFPAFTSR
KKAERIPGRLVVRRIPELNPKKAAGQGTLFDTHRFHAFFTTVDSGVLDTVAADKTHRQHAVIEQVNADLKDSALAHMPSGHFGANSAWLVAAAIAYNLTR
AAGILAGGTFAKARSATVRRKLIQVPARIARSARKTKLRLPLDWPWETGWERLFTSVHAPPQIA

 

Blast result :
Comments
ISArsp10 is 77% aa similar to ISCgl4.
References
1] Romaniuk K. (2018) Direct submission.