ISEhe4
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF324174 | ND | Erwinia herbicola | Erwinia herbicola pv. gypsophilae plasmid pPATH |
DNA section
IS Length : 1221 bp
Ends
IR Length : 13/15
IRL : TGACCTGCTCCCTGTTGATTAGTACACACCAATGTTAGTAATGTCTTCAT
IRR : TGACCTGCCCCCAGTATTAGATACAAGGCTCAGTTAGTAATGTCGGATCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TGACCTGCTCCCTGTTGATTAGTACACACCAATGTTAGTAATGTCTTCATAAGCCACATGAGGACATCCCCATGAAGAAGCGTTTTTCCGACGAACAGAT
CATCAGTATCCTCCGCGAGGCTGAAGCCGGGGTTTCTGCCCGGGAACTCTGCCGCAAGCATGCTATTTCAGACGCTACTTTTTACACCTGGCGCAAGAAG
TTTGGCGGCATGGAAGTCCCTGAGGTAAAGCGGCTTAAGTCACCTGAAGAGGAGAACGCCCGCCTTAAGAAGCTGCTTGCCGAAGCCATGCTGGATAAGG
AGGCGCTTCAGGTGGCTCTGGGTCGAAAGTTCTGACGACAGACCAGAAGCGGGAAGCCGTGGACGTCATGTGTGAGGTCGCCGGTCTGTCGCAACGTCGT
GCCTGCAGGCTGGCAGGTCTGTCTCTTTCAACCTGCCGTTATTCAGCTCAGCGTCCGGCTGCTGACGCGCAGCTATCTCTGCGCATCACAGAGCTGGCGC
CTGAACGCCGTCGCTTTGGTTATCGGCGGATCTGGCAGTTATTGCGTCGTGAGGGCCTTCACGTTAACCACAAGCGGGTATACCGCATTTATCATCTTAA
CGGCCTGAGTGTAAAGCGCAGACGACGTCGTAAGGGTCTGGCGACTGAGCGGCTTCCGCTGCTTCGCCCGGATTCGCCCAATCTGACCTGGTCAATGGAT
TTCGTCATGGATGCACTGGCCACCGGTCGCAGGATCAAGTGCCTTACCTGCGTCGATGACTTCACAAAGGAATGCCTGACTGTCACTGTTGCCTTTGGGA
TTTCAGGCGTGCAGGTCACGCGTATTCTGGACAGTATTGCGCTGTTTCGCGGCTATCCGGCTACGATAAGAACTGACCAGGGGCCGGAGTTTACCAGTAG
AGCACCTGACCAGTGGGCCTTTGAGCATTGCGTGGAGCTGCGGCTTATTCAGCCCGGTAAACCGACACAGAACGGATTTATTGAGAGTTTTAACGGACGC
TTTCGCGATGAATGCCTGAATGAACACTGGTTTAGCGACATCAGCCACGCCAGGAAAACCATCAGTGAATGGCGACAGGATTATAACGAATGTCGCCCAC
ACTCCACGCTGAATTATCAGACGCCGTCTGAATTTGCAGCGGGCTGGAGAAAGGGTAATTCTGAGAGTGAAGGATCCGACATTACTAACTGAGCCTTGTA
TCTAATACTGGGGGCAGGTCA
CATCAGTATCCTCCGCGAGGCTGAAGCCGGGGTTTCTGCCCGGGAACTCTGCCGCAAGCATGCTATTTCAGACGCTACTTTTTACACCTGGCGCAAGAAG
TTTGGCGGCATGGAAGTCCCTGAGGTAAAGCGGCTTAAGTCACCTGAAGAGGAGAACGCCCGCCTTAAGAAGCTGCTTGCCGAAGCCATGCTGGATAAGG
AGGCGCTTCAGGTGGCTCTGGGTCGAAAGTTCTGACGACAGACCAGAAGCGGGAAGCCGTGGACGTCATGTGTGAGGTCGCCGGTCTGTCGCAACGTCGT
GCCTGCAGGCTGGCAGGTCTGTCTCTTTCAACCTGCCGTTATTCAGCTCAGCGTCCGGCTGCTGACGCGCAGCTATCTCTGCGCATCACAGAGCTGGCGC
CTGAACGCCGTCGCTTTGGTTATCGGCGGATCTGGCAGTTATTGCGTCGTGAGGGCCTTCACGTTAACCACAAGCGGGTATACCGCATTTATCATCTTAA
CGGCCTGAGTGTAAAGCGCAGACGACGTCGTAAGGGTCTGGCGACTGAGCGGCTTCCGCTGCTTCGCCCGGATTCGCCCAATCTGACCTGGTCAATGGAT
TTCGTCATGGATGCACTGGCCACCGGTCGCAGGATCAAGTGCCTTACCTGCGTCGATGACTTCACAAAGGAATGCCTGACTGTCACTGTTGCCTTTGGGA
TTTCAGGCGTGCAGGTCACGCGTATTCTGGACAGTATTGCGCTGTTTCGCGGCTATCCGGCTACGATAAGAACTGACCAGGGGCCGGAGTTTACCAGTAG
AGCACCTGACCAGTGGGCCTTTGAGCATTGCGTGGAGCTGCGGCTTATTCAGCCCGGTAAACCGACACAGAACGGATTTATTGAGAGTTTTAACGGACGC
TTTCGCGATGAATGCCTGAATGAACACTGGTTTAGCGACATCAGCCACGCCAGGAAAACCATCAGTGAATGGCGACAGGATTATAACGAATGTCGCCCAC
ACTCCACGCTGAATTATCAGACGCCGTCTGAATTTGCAGCGGGCTGGAGAAAGGGTAATTCTGAGAGTGAAGGATCCGACATTACTAACTGAGCCTTGTA
TCTAATACTGGGGGCAGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 264 bp | 87 aa | 72 | 335 | + | No |
Description : First part of the transposase
ORF sequence :
MKKRFSDEQIISILREAEAGVSARELCRKHAISDATFYTWRKKFGGMEVPEVKRLKSPEEENARLKKLLAEAMLDKEALQVALGRKF
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 903 bp | 297 aa | 290 | 1192 | + | No |
Description : Second part of the transposase
ORF sequence :
GGASGGSGSKVLTTDQKREAVDVMCEVAGLSQRRACRLAGLSLSTCRYSAQRPAADAQLSLRITELAPERRRFGYRRIWQLLRREGLHVNHKRVYRIYHL
NGLSVKRRRRRKGLATERLPLLRPDSPNLTWSMDFVMDALATGRRIKCLTCVDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPATIRTDQGPEFTS
RAPDQWAFEHCVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDISHARKTISEWRQDYNECRPHSTLNYQTPSEFAAGWRKGNSESEGSDITN
NGLSVKRRRRRKGLATERLPLLRPDSPNLTWSMDFVMDALATGRRIKCLTCVDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPATIRTDQGPEFTS
RAPDQWAFEHCVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDISHARKTISEWRQDYNECRPHSTLNYQTPSEFAAGWRKGNSESEGSDITN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1121 bp | 313 aa | 72 | 1192 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKRFSDEQIISILREAEAGVSARELCRKHAISDATFYTWRKKFGGMEVPEVKRLKSPEEENARLKKLLAEAMLDKEALQVALGRKVLTTDQKREAVDVM
CEVAGLSQRRACRLAGLSLSTCRYSAQRPAADAQLSLRITELAPERRRFGYRRIWQLLRREGLHVNHKRVYRIYHLNGLSVKRRRRRKGLATERLPLLRP
DSPNLTWSMDFVMDALATGRRIKCLTCVDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPATIRTDQGPEFTSRAPDQWAFEHCVELRLIQPGKPTQ
NGFIESFNGRFRDECLNEHWFSDISHARKTISEWRQDYNECRPHSTLNYQTPSEFAAGWRKGNSESEGSDITN
CEVAGLSQRRACRLAGLSLSTCRYSAQRPAADAQLSLRITELAPERRRFGYRRIWQLLRREGLHVNHKRVYRIYHLNGLSVKRRRRRKGLATERLPLLRP
DSPNLTWSMDFVMDALATGRRIKCLTCVDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPATIRTDQGPEFTSRAPDQWAFEHCVELRLIQPGKPTQ
NGFIESFNGRFRDECLNEHWFSDISHARKTISEWRQDYNECRPHSTLNYQTPSEFAAGWRKGNSESEGSDITN
Blast result :
Comments
ISEhe4 is 98% aa similar to ORF1 of IS1222 and 93% to ORF2 of IS1222.
References
1] Guo,M., Mor,H.,Barash,I and Manulis,S. (2000) Direct submission GenBank.