ISArsp8

  • Family IS481
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
MH067975 ND Arthrobacter sp.
Arthrobacter sp.
Arthrobacter sp. ANT_H58 pA58H2
DNA section
IS Length : 1718 bp

Ends


IR Length : 35/50

IRL : TGTGATCTCTCAGAACATCGTTTACAACATCACTCAGGACATCGCTTACA
IRR : TGTGAACACTCGCTTCTTTGTTTACAGTCGCACTCACTACATCGTTTACA

Insertion site


Left flankDirect repeatRight flankDR Length
ACAATTCCGATGCTGCAAATGCCGACTCCAGAAAC15

DNA sequence

TGTGATCTCTCAGAACATCGTTTACAACATCACTCAGGACATCGCTTACAGGATCTCTCAAGGCATCGCTGACAGGTAGCAGTTGCGTGGCAGACCCGGA
TCTGACCTCGGATTATTCGATAATGGTGGGTGTCAAATACATTATCGGCGTCGGTACGCCGTCAAATCATTGAGTTCGATCCGGGTCTGCCAGACTCCCT
CAGTATCTCGCAATTCTGCGATCAACTGGGCATCAGCCGACCGTCCTATTACAAAGTCAAAAAACGCTATATCGCCGAAGGCAACAAGGCCCTTAACCCG
CATTCCCGGGCTCCGAAGAGCCCGGCGAGGACTTACGGGGAGCCAACCAAGGAATTGGTGTTGCGGATCCGTCAACGCATGGCCAAAGCCGGTTGGGACC
ACGGTCCGCAATCAATCTGGTTCGAAGGTGTGGACTCCGGAGAATTTGGGGAACAGGTCCCGTCGGTGGCAACCATTGGACGAATCCTTGCCGAATCGGG
CGTCACCAAAACCAACCCCCGAAAGCGACCGCGCAGTGCGTGGATGAGGTTCGCTCGGTCATATCCGATGGAGATGTGGCAGCTCGATGGGCTGGAATAT
CGGCTCTTCGATCAAGACGGAACCAAGGCAACGATCTACCAGCTCTTGGATGATGGAACCCGTTTTGATGTTGGATCGCTTTGCTTTGAACGACCCGAAA
ACAGCGAAGACGCGATCTCCACGTTGCGTGCAGCCTTCGACGAATACGGGGTTCCGCAGGAGCTTTTGACTGACAACGGACTCTCTTTCAACCAGGCCCG
TCGTGGAAAGATTAGCAGCACAGAGATCTTCCTAGCCGAGCGTGGTTGTCGGGGTATCACGGGCCGTATTTGGCATCCAGAAACCCAAGGAAAAAATGAA
AGAAGCCATCAGACCCTGGTCCGGTTTCTCGATGCTCACGCCCCTAAGAACCTCGATGATCTTTCCAAGCTTTTGGTGCGCTTCAGGGAGCACTACAACT
ATCGACGGCGTCATCAGTCTCTGAAAGTTGGAAGCACTCATCTCACCCCTGGCCAGGCATGGGAAGCAGGGGAACACCGGGGCTCCGACGGCACTCCCAT
TGGCATTGAGCAGTTGCAGGCCAAGGCTGATGCCTACCGCGACAAACAAATCGCTCTCAAAGCTGCTGCATCATCCACCGATACCGGTGGCACCATCATG
GCCACGCAGCAACAGGCCCAGGAAGCTTTGCCCGTCCAGACCGGCAAGCTGCAGGACCAGCCAAGTGACCTGGTGCAGATTACTCGAACCAACCCACAGA
TCTATTACCGTGGCCGTGCGTTTAAGGTGCCGACTCATCTGGTCGGCGAACACCGATTGGTCACCACCGAAACAGGATTTACGTTGTTCAGCACCCTTGA
TGGGGAGGAAAGTCTCTATTTTCCGTTGCCGCTGCGTCTTGCCTCCAGCAAAAAGCTGATGCCGCTGTGGCAGGTCCACGGTGCCCGCATCCGCGAACCG
AGACCGGCGTGGGTGCTGAAGCGGATCGACTACGAAAAGCAGCATTACCAGCTGGAACTTACCGGCTGACTTGACTGAAAATCTGAGTACACACGGTGTC
AGCGATGTTGTGAGTTTTGGTGTCAACGATGTGCTGACATCGTGTGTAAAGGAAGTGTCGAGTGCGTGTGTAAACGATGTAGTGAGTGCGACTGTAAACA
AAGAAGCGAGTGTTCACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1440 bp479 aa1301569+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

GTGTCAAATACATTATCGGCGTCGGTACGCCGTCAAATCATTGAGTTCGATCCGGGTCTGCCAGACTCCCTCAGTATCTCGCAATTCTGCGATCAACTGG
GCATCAGCCGACCGTCCTATTACAAAGTCAAAAAACGCTATATCGCCGAAGGCAACAAGGCCCTTAACCCGCATTCCCGGGCTCCGAAGAGCCCGGCGAG
GACTTACGGGGAGCCAACCAAGGAATTGGTGTTGCGGATCCGTCAACGCATGGCCAAAGCCGGTTGGGACCACGGTCCGCAATCAATCTGGTTCGAAGGT
GTGGACTCCGGAGAATTTGGGGAACAGGTCCCGTCGGTGGCAACCATTGGACGAATCCTTGCCGAATCGGGCGTCACCAAAACCAACCCCCGAAAGCGAC
CGCGCAGTGCGTGGATGAGGTTCGCTCGGTCATATCCGATGGAGATGTGGCAGCTCGATGGGCTGGAATATCGGCTCTTCGATCAAGACGGAACCAAGGC
AACGATCTACCAGCTCTTGGATGATGGAACCCGTTTTGATGTTGGATCGCTTTGCTTTGAACGACCCGAAAACAGCGAAGACGCGATCTCCACGTTGCGT
GCAGCCTTCGACGAATACGGGGTTCCGCAGGAGCTTTTGACTGACAACGGACTCTCTTTCAACCAGGCCCGTCGTGGAAAGATTAGCAGCACAGAGATCT
TCCTAGCCGAGCGTGGTTGTCGGGGTATCACGGGCCGTATTTGGCATCCAGAAACCCAAGGAAAAAATGAAAGAAGCCATCAGACCCTGGTCCGGTTTCT
CGATGCTCACGCCCCTAAGAACCTCGATGATCTTTCCAAGCTTTTGGTGCGCTTCAGGGAGCACTACAACTATCGACGGCGTCATCAGTCTCTGAAAGTT
GGAAGCACTCATCTCACCCCTGGCCAGGCATGGGAAGCAGGGGAACACCGGGGCTCCGACGGCACTCCCATTGGCATTGAGCAGTTGCAGGCCAAGGCTG
ATGCCTACCGCGACAAACAAATCGCTCTCAAAGCTGCTGCATCATCCACCGATACCGGTGGCACCATCATGGCCACGCAGCAACAGGCCCAGGAAGCTTT
GCCCGTCCAGACCGGCAAGCTGCAGGACCAGCCAAGTGACCTGGTGCAGATTACTCGAACCAACCCACAGATCTATTACCGTGGCCGTGCGTTTAAGGTG
CCGACTCATCTGGTCGGCGAACACCGATTGGTCACCACCGAAACAGGATTTACGTTGTTCAGCACCCTTGATGGGGAGGAAAGTCTCTATTTTCCGTTGC
CGCTGCGTCTTGCCTCCAGCAAAAAGCTGATGCCGCTGTGGCAGGTCCACGGTGCCCGCATCCGCGAACCGAGACCGGCGTGGGTGCTGAAGCGGATCGA
CTACGAAAAGCAGCATTACCAGCTGGAACTTACCGGCTGA

 

Blast result :
Comments
ISArps8 is 81% aa similar to ISAar22.
References
1] Krzysztof Romaniuk (2018) Direct submission.