ISEc83

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Escherichia coli
Escherichia coli
Escherichia coli O121:H19 51104
DNA section
IS Length : 2709 bp

Ends


IR Length : 24/34

IRL : GTAAGCGCCCCATCTGCGACGTCTTGTGAAAATTgtcctgtctggcaaca
IRR : gtaagcgcaccgtgaaggacgtggggtaaaaaTTAGTTTACAGATTGAGT

Insertion site


Left flankDirect repeatRight flankDR Length
ggttcagaccctttttttaatgatgatg8
tttacctgtatgacctacgccgcatgga8
agacagtgacggatgttgtcaagatatt8
ggacatgccattgttttctgactgttgg8
cggcaactgacagaaatctcagcaatga8
ctgttgttcaaatggcgacaacaatggc8
tagagtgcgattgctgtgaacaacagac8

DNA sequence

GTAAGCGCCCCATCTGCGACGTCTTGTGAAAATTgtcctgtctggcaacaatcgcgcccatctatatagatggacacgaacgATGAATTCCCAGACAAAA
AAAGATATTCCCTGCTTCCGTTCTTATTTGCCTGATGCTCTGCGTTTAAGATTTGAAGATAAACTGACCATCCGGGCCATCGCTCAGCGTCTAGGTCTCA
GTCATTCCACAATACATACGCTTTTTCAGCGATTTATTGCATCCGGTATCGCATGGCCATTGCCCGATTCAGTTTCATTAGCTCAGCTTGATGCCATCCT
TTATGCCAACAGAAAGAAGGAATTAACAGAGCCTCAAATCAGCGAAGGCACATGGCGAAAAGAACGGCGAGCCAGCTACAGCCGTGAATTTAAGGTCCGT
CTGGCTAAGCAGGCATTACAGCCCGGTGCTGTTGTTGCCCGGATCGCCAGAGAGCACGGTATCAATGATAACCTGCTGTTTAAATGGAAAAGCCAGTACG
AGGACGGCTTACTGAGCGATGATGACATACAGGAATGCATGCCTGTCCCGGTGGCTCTGACTGATACGCCAGAGCCGACCAGACCAGTTACAAATCCCTT
CTGGCGTAACAAGCCTGATGAGTGCCCTGAGAGTGATCCCGGAAACGTCCCACGGTGCGAGCTGCATCTTAAATCAGGTGTGGTAAAACTGTTTGACCCT
CTCACTCCGGAAATGTTACGGGCGCTAATCCGCGAAATGAAAGGAGGTACCCGATGAtaacgctgccaaccggtaccagaatctggatcatcgctggcat
cacagatatgcgttgtggcttcaacggcctggcttcgaaggtgcagaacacgctgaaagatgacccgttctccgggcatatcttcgtcttccggggccgc
agtggcaaaatggtgaaaatactgtgggccgatcgtgacgggttatgcctgttcgccaaacgcctggaacggggccgcttcgtctggccggtaacccggg
aagggaaagtgcacctgacgccagctcagttatccatgctactggaggggatcgcgtggcaacatcccaaacggacagaacggcctggcatccggataTA
AcccgtgataaaacaggggaATGAACAACACACTCCCCGACGACATCGAGCAACTGAAGGCCCTGCTGATCGCACAGCAGGCTGTTATCGTCCGTCTGTC
TGGTGAAATAACCGGCTATGCCCGCGAGATCAGCTCACTCAGAGCGCTGGTCGCTAAACTGCAGAGAATGTTGTTCGGTCGCAGCAGCGAGAAAAGCCGC
GAGAAGACAGAAAAGAAGATCGCACGGGCAGAAACGCGTATAACCGAGCTCCAGAACAGGCTTGGTGAGGCGCAGTTGCAACTCACCTCAATGGCCGGAG
AGACAGCGCCGAAAACATCAGACTCTCCCGTCCGCAAAGCACTTCCGGCAACACTTCCCCGTGACAGGCAGGTTATCTCCCCGGCAGAAACCGAATGCCC
CGTCTGCAGCGGCAAACTGAAACCGCTGGGAGAAAGCATCTCTGAACAACTGGATATCATCAACACCGCGTTCAGGGTAATCGAAACGGTTCGCCCAAAA
CTGGCCTGCAGCCGGTGCGACTGTATAGTTCAGGCTCCGCAGCCACCAAAACCCATCGAGCGCAGTTACGCCAGTCCGGCTCTGCTGGCCCGCATAATCA
TGGCTAAGTTCGCCGAGCATCTGCCGCTGTACCGTCAGTCGGAAATCTATGCCCGCCAGGGCGTGGAGCTGCACCGCAATACGATGGGGCGCTGGGTTGA
CATCATGGGAGAGCAGCTTCGCCCGCTGTATGATGAACTGAAGCACTATGTGCTGATGGCGGGTAAAGTGCATGCCGATGACACGCCGGTAAATGTACTG
GAGCCGGGTCAGGGTAAAACCCGTACCGGACGGCTGTGGGTCTATGTTCGTGACGATCGCAACGCCGGTTCGACCATGCCGGCAGCGGTGTGGTTCTCAT
ACTCTCCCGACCGCAAAGGCATCCACCCACAGCAACATCTGGCGGACTACAGAGGTATCCTGCAGGCCGATGCATATGCGGGTTACAATGCTCTTTACGA
AAGCGGTCAGGTAACCGAAGCGGCTTGTATGGCACATGCCCGACGCAAGATCCACGATGTACATGTCCGCCATCCAACGACAGTAACGGGAGAAGCGCTC
CGTCGTATCGGGGAACTGTACGCTATCGAGGCTGAGATCCGCGGCAGTCCGGCAGAAGAGCGACTGGCGGTCAGAAAAGCCAGAACGGTACCGCTAATGC
AGTCGTTGTATGAGTGGCTCCAGGGGCAGATGAGCACGCTGTCGCGCCACTCGGATACAGCGAAAGCGTTCACCTATCTGCTGAAGCAATGGGACGCTCT
GAACGAATACTGCCGCAATGGCTGGGTGGAGATCGACAATAACCTGTGTGAAAACGCCCTCCGGGTAGTTGCACTGGGGCGGCGTAACTACATGTTCTTC
GGCTCTGATGGTGGAGGCGAGAGTGCGGCAGTGATGTACAGCCTGATCGGTAGCTGCAAACTTAACGGAATCGAGCCGGAAACGTGGCTACGCCACGTGA
TCAGTGTAATCAACACCTGGCCTGCCAACCGCGTGAAAGAGTTGTTGCCCTGGAATGTCACTCAATCTGTAAACTAAtttttaccccacgtccttcacgg
tgcgcttac
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
675 bp224 aa83757+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTIHTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRASYS
REFKVRLAKQALQPGAVVARIAREHGINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGV
VKLFDPLTPEMLRALIREMKGGTR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
348 bp115 aa7541101+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIFVFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGI
AWQHPKRTERPGIRI

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1557 bp518 aa11212677+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNNTLPDDIEQLKALLIAQQAVIVRLSGEITGYAREISSLRALVAKLQRMLFGRSSEKSREKTEKKIARAETRITELQNRLGEAQLQLTSMAGETAPKTS
DSPVRKALPATLPRDRQVISPAETECPVCSGKLKPLGESISEQLDIINTAFRVIETVRPKLACSRCDCIVQAPQPPKPIERSYASPALLARIIMAKFAEH
LPLYRQSEIYARQGVELHRNTMGRWVDIMGEQLRPLYDELKHYVLMAGKVHADDTPVNVLEPGQGKTRTGRLWVYVRDDRNAGSTMPAAVWFSYSPDRKG
IHPQQHLADYRGILQADAYAGYNALYESGQVTEAACMAHARRKIHDVHVRHPTTVTGEALRRIGELYAIEAEIRGSPAEERLAVRKARTVPLMQSLYEWL
QGQMSTLSRHSDTAKAFTYLLKQWDALNEYCRNGWVEIDNNLCENALRVVALGRRNYMFFGSDGGGESAAVMYSLIGSCKLNGIEPETWLRHVISVINTW
PANRVKELLPWNVTQSVN

 

Blast result :
Comments
ISEc83 is 79% aa (transposase) similar to ISSfl3.
References
1] Keiji Nakamura (2018) Direct submission.