ISEhe3
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF327445 | ND | Pantoea agglomerans | Pantoea agglomerans plasmid pPATH |
DNA section
IS Length : 1229 bp
Ends
IR Length : 16/20
IRL : TGAATCGCCACGAGTTTAACAGACACCTCAGAGCCATTTAAGAGGACTTA
IRR : TGAATCGCCACGGATAATCTAGACACTTCTGAGCCGTTGATAATACTGGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| NNNNNGTAAT | AAC | ATTGCNNNNN | 3 |
DNA sequence
TGAATCGCCACGAGTTTAACAGACACCTCAGAGCCATTTAAGAGGACTTAAAAGAGAGGTGCCCATTAGCGGTAAGCGTTATCCCGAAGAGTTTAAAATT
GAAGCAGTCAAACAGGTTGTTGATCGTGGTCATTCTGTTTCCAGCGTTGCAACACGTCTCGATATCACCACCCACAGCCTTTACGCCTGGATAAAGAAGT
ACGGTCCGGATTCTTCCATTAATAAAGAACAGTCAGATGCTCAGGCCGAGATCCGCCGTCTCCAGAACGAGCTGAAGCGGGTTACCGACGGACGGGACAT
ATTAAAAAAAGCCGCGGCGTACTTCGCAAAGCTGTCCGACTGAGGTACGCCTTTATCCGTGACAACACTTTTTGCGGCCTGTTCGCCTGCTCTGTCGGGT
GCTGGATGTTCATCCGGGTGGCTTTTACGCCTGGCTTCAGCAGCCGCATTCACAACGCCATCAGGCAGACCTGAGACTGACAGGACAGATAAAACAGTTC
TGGCTGGAATCGGGATGCGTCTATGGTTATCGCAAGATCTATCTGAAGCTGCGGGACAGCGGGCAACAGTGCGGAGTCAACAGAGTCTGGCGACTGATGA
AACGTGTCGGGATAAAGGCTCAGGTCGGGTACCGGAGCCCGCGGGCACGTAAAGGCGAGGCCAGTATTGTGTCGCCCAACAGGCTCCGGCGGCAGTTCAA
TTCGGATGCTCCGGATGAGCGTTGGGTAACGGACATAACCTACATCAGGACCCACGAAGGTTGGTTGTATCTGGCCGTGGTTGTTGATCTGTTCTCACGC
AAAATTATCGGCTGGTCCATGCAATCCCGGATGACAAAGGACATTGTCCTGAACGCACTGCTGATGGCTGTATGGCGGCGTAATCCCCAAAAACAGGTGT
TGGTTCACTCGGATCAGGGCAGTCAGTACACAAGCCATGAGTGGCAGTCGTTCCTGGAATCACACGGCCTGGAGGGCAGCATGAGCCGTCGCGGTAACTG
CCACGATAATGCGGTTGCAGAAAGTTTTTTCCAGTTATTGAAACGCGAGCGGATAAAGAAAAAGATCTACGGAACGCGGGAAGAAGCACGCAGCGATATT
TTTGATTACATCAAAATGTTTTATAACAGTAAGCGTCGGCATGGTTCGAGCGATCAGATGTCCCCGACAGAATATGAAAACCAGTATTATCAACGGCTCA
GAAGTGTCTAGATTATCCGTGGCGATTCA
GAAGCAGTCAAACAGGTTGTTGATCGTGGTCATTCTGTTTCCAGCGTTGCAACACGTCTCGATATCACCACCCACAGCCTTTACGCCTGGATAAAGAAGT
ACGGTCCGGATTCTTCCATTAATAAAGAACAGTCAGATGCTCAGGCCGAGATCCGCCGTCTCCAGAACGAGCTGAAGCGGGTTACCGACGGACGGGACAT
ATTAAAAAAAGCCGCGGCGTACTTCGCAAAGCTGTCCGACTGAGGTACGCCTTTATCCGTGACAACACTTTTTGCGGCCTGTTCGCCTGCTCTGTCGGGT
GCTGGATGTTCATCCGGGTGGCTTTTACGCCTGGCTTCAGCAGCCGCATTCACAACGCCATCAGGCAGACCTGAGACTGACAGGACAGATAAAACAGTTC
TGGCTGGAATCGGGATGCGTCTATGGTTATCGCAAGATCTATCTGAAGCTGCGGGACAGCGGGCAACAGTGCGGAGTCAACAGAGTCTGGCGACTGATGA
AACGTGTCGGGATAAAGGCTCAGGTCGGGTACCGGAGCCCGCGGGCACGTAAAGGCGAGGCCAGTATTGTGTCGCCCAACAGGCTCCGGCGGCAGTTCAA
TTCGGATGCTCCGGATGAGCGTTGGGTAACGGACATAACCTACATCAGGACCCACGAAGGTTGGTTGTATCTGGCCGTGGTTGTTGATCTGTTCTCACGC
AAAATTATCGGCTGGTCCATGCAATCCCGGATGACAAAGGACATTGTCCTGAACGCACTGCTGATGGCTGTATGGCGGCGTAATCCCCAAAAACAGGTGT
TGGTTCACTCGGATCAGGGCAGTCAGTACACAAGCCATGAGTGGCAGTCGTTCCTGGAATCACACGGCCTGGAGGGCAGCATGAGCCGTCGCGGTAACTG
CCACGATAATGCGGTTGCAGAAAGTTTTTTCCAGTTATTGAAACGCGAGCGGATAAAGAAAAAGATCTACGGAACGCGGGAAGAAGCACGCAGCGATATT
TTTGATTACATCAAAATGTTTTATAACAGTAAGCGTCGGCATGGTTCGAGCGATCAGATGTCCCCGACAGAATATGAAAACCAGTATTATCAACGGCTCA
GAAGTGTCTAGATTATCCGTGGCGATTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 285 bp | 94 aa | 67 | 351 | + | No |
Description : First part of the transposase
ORF sequence :
MPISGKRYPEEFKIEAVKQVVDRGHSVSSVATRLDITTHSLYAWIKKYGPDSSINKEQSDAQAEIRRLQNELKRVTDGRDILKKAAAYFAKLSD
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 916 bp | 301 aa | 304 | 1219 | + | No |
Description : Second part of the transposase
ORF sequence :
KKPRRTSQSCPTEVRLYP*QHFLRPVRLLCRVLDVHPGGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIYLKLRDSGQQCGVNRVWRLMKR
VGIKAQVGYRSPRARKGEASIVSPNRLRRQFNSDAPDERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPQKQVLV
HSDQGSQYTSHEWQSFLESHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREEARSDIFDYIKMFYNSKRRHGSSDQMSPTEYENQYYQRLRS
V
VGIKAQVGYRSPRARKGEASIVSPNRLRRQFNSDAPDERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPQKQVLV
HSDQGSQYTSHEWQSFLESHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREEARSDIFDYIKMFYNSKRRHGSSDQMSPTEYENQYYQRLRS
V
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1153 bp | 384 aa | 67 | 1219 | + | Yes |
Chemistry : DDE
ORF sequence :
MPISGKRYPEEFKIEAVKQVVDRGHSVSSVATRLDITTHSLYAWIKKYGPDSSINKEQSDAQAEIRRLQNELKRVTDGRDILKKKPRRTSQSCPTEVRLY
P*QHFLRPVRLLCRVLDVHPGGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIYLKLRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKG
EASIVSPNRLRRQFNSDAPDERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPQKQVLVHSDQGSQYTSHEWQSFL
ESHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREEARSDIFDYIKMFYNSKRRHGSSDQMSPTEYENQYYQRLRSV
P*QHFLRPVRLLCRVLDVHPGGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIYLKLRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKG
EASIVSPNRLRRQFNSDAPDERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPQKQVLVHSDQGSQYTSHEWQSFL
ESHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREEARSDIFDYIKMFYNSKRRHGSSDQMSPTEYENQYYQRLRSV
Blast result :
Comments
ISEhe3 is 58% aa similar to ORF1 of ISSfl7 and 60% to ORF2 of ISRso8.
The first and last 8 nucleotides of the submitted sequence have been removed to generate correct ends for an IS3 family member. The deleted bases included an obvious 3 pb DR.
The first and last 8 nucleotides of the submitted sequence have been removed to generate correct ends for an IS3 family member. The deleted bases included an obvious 3 pb DR.
References
1] Guo,M., Mor,H., Manulis,S. and Barash,I (2000) Direct submission GenBank.