ISEcl10
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| MF344572 | ND | Enterobacter cloacae | Enterobacter cloacae 24845 plasmid p24845-AZM |
DNA section
IS Length : 1207 bp
Ends
IR Length : 30/44
IRL : TGTAAGTATCCCGCATAATCGTGCCATTCACATTTAGAGATCCTCCGGCA
IRR : TGTAAATAGACCCGTTTTAGTTCCAGGCATTTTTTGAGATCCCGGCCAAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTGTGATTTT | ATCT | CCCCGCGCCT | 4 |
DNA sequence
TGTAAGTATCCCGCATAATCGTGCCATTCACATTTAGAGATCCTCCGGCATAATCACTCTGCCAACAAAGGAGATCGCTATGCGTAAAGCCCGTTTTACT
GAGCATCAGATCATCGCCGTGATTAAGTCGGTTGAAGCCGGACGAACGGTTAAGGATGTCTGCCGGGAGGCCGGTATCTCTGAAGCGACTTACTACAACT
GGAAGTCTAAATACGGCGGTATGGAAGCTTCTGATATTAAAAAGATCAAGGAGCTTGAGGACGAGAACCGACGCCTCAAACAAATGTTTGCCGACCTGAG
CCTTGAGAACCGGGCGCTGAAAGACGTTATCGAAAAAAAGCTTTAAAACCAGCCTTTAAGCGTGAGCTGGTCACTCATCTGATAACGGCATTCGGACTCA
GTATCCGTCAGGCCTGTCGGAGTCTCAATCTGAGCAGAACGGTTTATCATTACCGTCCGGATACCACGCGTGATGAACCCGTTATTACCGCATTGCAGGC
GGCAGCTGAACGATACCCACGATACGGTTTTCCAAAACTTTTCCAGGTTCTGCGGCGGCAGGGATACGCGTGGAATCACAAAAGGATCCATCGTATTTAT
TGTCTGCTGAAGCTGAATTTTCGCCGTAAGGGTAAACAACGGCTGCCGGTGCGTAATCCCTCGCCACTGGCCACACCGGAAGCGCTGAACCAGAGTTGGT
CTGTCGATTTTATGCATGATGCCCTGGTCTGTGGCCGTCGTTTTCGCACGTTCAATGTCGTTGATGACTTTAACCGTGAGGCGTTGTCGATTGAAATAGA
TCTGAATCTGCCAGCTCCGCGAGTGGTCCGTGTGCTCGACAGGATCGCGGCAAATCGCGGCTATCCGCTCATGCTGCGCATGGATAATGGACCGGAATTT
ATCTCGCTGGCACTGGCTGAATGGGCAGAGAAACATGCAGTAAAGCTGGAGTTTATCCAGCCGGGTAAGCCGACACAGAACGCTTTTATTGAGCGTTTTA
ACAGGACATATCGCACAGAAATACTCGATTTTTATCTGTTCAGAACGCTGAATGAAGTGCGGGAAATCACGGAAAGATGGGTGTCAGAATATAACTGTGA
ACGCCCTCATGAATCACTGAACAATATGACGCCGGAGGAATATCGGCAACACAATCATTTGGCCGGGATCTCAAAAAATGCCTGGAACTAAAACGGGTCT
ATTTACA
GAGCATCAGATCATCGCCGTGATTAAGTCGGTTGAAGCCGGACGAACGGTTAAGGATGTCTGCCGGGAGGCCGGTATCTCTGAAGCGACTTACTACAACT
GGAAGTCTAAATACGGCGGTATGGAAGCTTCTGATATTAAAAAGATCAAGGAGCTTGAGGACGAGAACCGACGCCTCAAACAAATGTTTGCCGACCTGAG
CCTTGAGAACCGGGCGCTGAAAGACGTTATCGAAAAAAAGCTTTAAAACCAGCCTTTAAGCGTGAGCTGGTCACTCATCTGATAACGGCATTCGGACTCA
GTATCCGTCAGGCCTGTCGGAGTCTCAATCTGAGCAGAACGGTTTATCATTACCGTCCGGATACCACGCGTGATGAACCCGTTATTACCGCATTGCAGGC
GGCAGCTGAACGATACCCACGATACGGTTTTCCAAAACTTTTCCAGGTTCTGCGGCGGCAGGGATACGCGTGGAATCACAAAAGGATCCATCGTATTTAT
TGTCTGCTGAAGCTGAATTTTCGCCGTAAGGGTAAACAACGGCTGCCGGTGCGTAATCCCTCGCCACTGGCCACACCGGAAGCGCTGAACCAGAGTTGGT
CTGTCGATTTTATGCATGATGCCCTGGTCTGTGGCCGTCGTTTTCGCACGTTCAATGTCGTTGATGACTTTAACCGTGAGGCGTTGTCGATTGAAATAGA
TCTGAATCTGCCAGCTCCGCGAGTGGTCCGTGTGCTCGACAGGATCGCGGCAAATCGCGGCTATCCGCTCATGCTGCGCATGGATAATGGACCGGAATTT
ATCTCGCTGGCACTGGCTGAATGGGCAGAGAAACATGCAGTAAAGCTGGAGTTTATCCAGCCGGGTAAGCCGACACAGAACGCTTTTATTGAGCGTTTTA
ACAGGACATATCGCACAGAAATACTCGATTTTTATCTGTTCAGAACGCTGAATGAAGTGCGGGAAATCACGGAAAGATGGGTGTCAGAATATAACTGTGA
ACGCCCTCATGAATCACTGAACAATATGACGCCGGAGGAATATCGGCAACACAATCATTTGGCCGGGATCTCAAAAAATGCCTGGAACTAAAACGGGTCT
ATTTACA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 80 | 346 | + | No |
Description : First part of the transposase
ORF sequence :
MRKARFTEHQIIAVIKSVEAGRTVKDVCREAGISEATYYNWKSKYGGMEASDIKKIKELEDENRRLKQMFADLSLENRALKDVIEKKL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 885 bp | 294 aa | 307 | 1191 | + | No |
Description : Second part of the transposase
ORF sequence :
EPGAERRYRKKALKPAFKRELVTHLITAFGLSIRQACRSLNLSRTVYHYRPDTTRDEPVITALQAAAERYPRYGFPKLFQVLRRQGYAWNHKRIHRIYCL
LKLNFRRKGKQRLPVRNPSPLATPEALNQSWSVDFMHDALVCGRRFRTFNVVDDFNREALSIEIDLNLPAPRVVRVLDRIAANRGYPLMLRMDNGPEFIS
LALAEWAEKHAVKLEFIQPGKPTQNAFIERFNRTYRTEILDFYLFRTLNEVREITERWVSEYNCERPHESLNNMTPEEYRQHNHLAGISKNAWN
LKLNFRRKGKQRLPVRNPSPLATPEALNQSWSVDFMHDALVCGRRFRTFNVVDDFNREALSIEIDLNLPAPRVVRVLDRIAANRGYPLMLRMDNGPEFIS
LALAEWAEKHAVKLEFIQPGKPTQNAFIERFNRTYRTEILDFYLFRTLNEVREITERWVSEYNCERPHESLNNMTPEEYRQHNHLAGISKNAWN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1112 bp | 370 aa | 80 | 1191 | + | Yes |
Chemistry : DDE
ORF sequence :
MRKARFTEHQIIAVIKSVEAGRTVKDVCREAGISEATYYNWKSKYGGMEASDIKKIKELEDENRRLKQMFADLSLENRALKDVIEKKALKPAFKRELVTH
LITAFGLSIRQACRSLNLSRTVYHYRPDTTRDEPVITALQAAAERYPRYGFPKLFQVLRRQGYAWNHKRIHRIYCLLKLNFRRKGKQRLPVRNPSPLATP
EALNQSWSVDFMHDALVCGRRFRTFNVVDDFNREALSIEIDLNLPAPRVVRVLDRIAANRGYPLMLRMDNGPEFISLALAEWAEKHAVKLEFIQPGKPTQ
NAFIERFNRTYRTEILDFYLFRTLNEVREITERWVSEYNCERPHESLNNMTPEEYRQHNHLAGISKNAWN
LITAFGLSIRQACRSLNLSRTVYHYRPDTTRDEPVITALQAAAERYPRYGFPKLFQVLRRQGYAWNHKRIHRIYCLLKLNFRRKGKQRLPVRNPSPLATP
EALNQSWSVDFMHDALVCGRRFRTFNVVDDFNREALSIEIDLNLPAPRVVRVLDRIAANRGYPLMLRMDNGPEFISLALAEWAEKHAVKLEFIQPGKPTQ
NAFIERFNRTYRTEILDFYLFRTLNEVREITERWVSEYNCERPHESLNNMTPEEYRQHNHLAGISKNAWN
Blast result :
Comments
ISEcl10 is 97% aa similar to IS1351.
The third ORf is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORf is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Dongsheng Zhou (2018) Direct submission.
2] Luo,W., Feng,J., Zeng,L., Jiang,X., Zhan,Z., Zhang,D., Zhao,Y. and Zhou,D. (2017) Direct GenBank submission.
2] Luo,W., Feng,J., Zeng,L., Jiang,X., Zhan,Z., Zhang,D., Zhao,Y. and Zhou,D. (2017) Direct GenBank submission.