ISCfr3
- Family ISNCY
- Group ISPlu15
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Citrobacter freundii | Citrobacter freundii Klebsiella pneumoniae 160111 plasmid pIncFII_L111 |
DNA section
IS Length : 1081 bp
Ends
IR Length : 14/19
IRL : TCCGTTCACCGCCAATATTAGCTTTTCCCGGGCTGTGATAACATCTCCGG
IRR : TCCCTGCCCCACCAGTATTCTCTGATGGCCGGACAGGGAGTGCCAGGAAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCTGGCAGGCAGAGCCTGAGTCTGGTGCC | ATTGCCATTCCTTCAGGGGACCGTTCCGGG | 0 |
DNA sequence
TCCGTTCACCGCCAATATTAGCTTTTCCCGGGCTGTGATAACATCTCCGGGTCATTTTTCAGAGCCCAGGACTGATGAAGAAGAAACCAGGTACACCCAC
ACCCCATGACGCGACATTTCGCCAGTTCCTGGCGAATCCCGATGTCGCCCGTGACTTCATGATGTTACATCTCCCGGCTGAATTACGCGCGCTGTGCGAT
TTGACCACGCTGAAGCTGGAGTCCGGTACCTTCGTTGAGGACGACTTACGTCAGTACGTCAGCGACATTCTCTGGAGCATGAAAACCACCACCGGCGATG
ACGGGTACATCCATCTGCTTCTGGAACACCAGAGTTCGGCGGATAAAAATATGGCCTTTCGTCAGCTCAGATATGCTGTCGCCGCCATGCAACGTCATCT
TGAAGCGGGTCATAAAAAACTGCCGCTGGTCATCCCGGTTCTGTTTTACCACGGCAGCCGGAGTCCGTACCCGTTCTCCACGAACTGGCTGGATTGTTTC
AGTAACCCGGAACAGGCCGGAAAAGTATACACGACAGGATTCCCGCTGGTTGATGTCACCGTCATCGATGATGATGAGATAATGAACCACAGACGGATGG
CCGCACTGACCCTGCTGATGAAACATATCCGCCAGCGGGACATGATGGAACTGCTGGACAGGCTACCCCGGGTAATGGTGGAATGGATTTCCCCCGAACA
GGTGCGCGTTCTGATAAACTACATGGTAAATGCAGGTGAAGCGCCTGCACCTGAATTTATGCGGGCGCTGGCAGAGCGTCTGCCGCAACACGAGGATGAA
CTGATGACCATCGCACAACAGCTTGAACAGATTGGCATCGAGAAGGGCCTGCAGCAGGGTCTGCAGCAGGGCCTGCAGTTAGGTGAACAACGCGGTATTG
AAAAAGGCGAGCGCGAAGCCACCCTTAAAATTGCACGAACCATGCTGCAGAACGGAATTGACCGCAATACCGTCATGAAAATGACCGGTCTGACTGAAGA
CGAACTAGCGCAGATCCGCCACTAATCCCCCTTTCCTGGCACTCCCTGTCCGGCCATCAGAGAATACTGGTGGGGCAGGGA
ACCCCATGACGCGACATTTCGCCAGTTCCTGGCGAATCCCGATGTCGCCCGTGACTTCATGATGTTACATCTCCCGGCTGAATTACGCGCGCTGTGCGAT
TTGACCACGCTGAAGCTGGAGTCCGGTACCTTCGTTGAGGACGACTTACGTCAGTACGTCAGCGACATTCTCTGGAGCATGAAAACCACCACCGGCGATG
ACGGGTACATCCATCTGCTTCTGGAACACCAGAGTTCGGCGGATAAAAATATGGCCTTTCGTCAGCTCAGATATGCTGTCGCCGCCATGCAACGTCATCT
TGAAGCGGGTCATAAAAAACTGCCGCTGGTCATCCCGGTTCTGTTTTACCACGGCAGCCGGAGTCCGTACCCGTTCTCCACGAACTGGCTGGATTGTTTC
AGTAACCCGGAACAGGCCGGAAAAGTATACACGACAGGATTCCCGCTGGTTGATGTCACCGTCATCGATGATGATGAGATAATGAACCACAGACGGATGG
CCGCACTGACCCTGCTGATGAAACATATCCGCCAGCGGGACATGATGGAACTGCTGGACAGGCTACCCCGGGTAATGGTGGAATGGATTTCCCCCGAACA
GGTGCGCGTTCTGATAAACTACATGGTAAATGCAGGTGAAGCGCCTGCACCTGAATTTATGCGGGCGCTGGCAGAGCGTCTGCCGCAACACGAGGATGAA
CTGATGACCATCGCACAACAGCTTGAACAGATTGGCATCGAGAAGGGCCTGCAGCAGGGTCTGCAGCAGGGCCTGCAGTTAGGTGAACAACGCGGTATTG
AAAAAGGCGAGCGCGAAGCCACCCTTAAAATTGCACGAACCATGCTGCAGAACGGAATTGACCGCAATACCGTCATGAAAATGACCGGTCTGACTGAAGA
CGAACTAGCGCAGATCCGCCACTAATCCCCCTTTCCTGGCACTCCCTGTCCGGCCATCAGAGAATACTGGTGGGGCAGGGA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 951 bp | 316 aa | 105 | 1055 | + | No |
Chemistry : Unknow
ORF sequence :
MKKKPGTPTPHDATFRQFLANPDVARDFMMLHLPAELRALCDLTTLKLESGTFVEDDLRQYVSDILWSMKTTTGDDGYIHLLLEHQSSADKNMAFRQLRY
AVAAMQRHLEAGHKKLPLVIPVLFYHGSRSPYPFSTNWLDCFSNPEQAGKVYTTGFPLVDVTVIDDDEIMNHRRMAALTLLMKHIRQRDMMELLDRLPRV
MVEWISPEQVRVLINYMVNAGEAPAPEFMRALAERLPQHEDELMTIAQQLEQIGIEKGLQQGLQQGLQLGEQRGIEKGEREATLKIARTMLQNGIDRNTV
MKMTGLTEDELAQIRH
AVAAMQRHLEAGHKKLPLVIPVLFYHGSRSPYPFSTNWLDCFSNPEQAGKVYTTGFPLVDVTVIDDDEIMNHRRMAALTLLMKHIRQRDMMELLDRLPRV
MVEWISPEQVRVLINYMVNAGEAPAPEFMRALAERLPQHEDELMTIAQQLEQIGIEKGLQQGLQQGLQLGEQRGIEKGEREATLKIARTMLQNGIDRNTV
MKMTGLTEDELAQIRH
Blast result :
Comments
ISCfr3 is 84% aa similar to ISRor2.
It was difficult to determine exactly the ends of the element, this sequence is inserted always at the same place in different plasmids of enterobacteria. We have not found empty site to comfort the sequence ends determination.
It was difficult to determine exactly the ends of the element, this sequence is inserted always at the same place in different plasmids of enterobacteria. We have not found empty site to comfort the sequence ends determination.
References
1] Dongsheng Zhou (2016) Direct submission.