ISEhe2
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF327444 | ND | Pantoea agglomerans | Pantoea agglomerans plasmid pPATH |
DNA section
IS Length : 929 bp
Ends
IR Length : 22/23
IRL : CACACTAGGCTGTGTCCCTTAATCACCTGAGCTATAATAACTGCCTGTTT
IRR : CAACTAGGCTGTGTCCCTTAATTGCATAACCTTCTGTAAAACAGGCGGAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
CACACTAGGCTGTGTCCCTTAATCACCTGAGCTATAATAACTGCCTGTTTCCACAGCATATTGAACTATGGCTCGCTACGACATTCCTGATGAAGCATGG
GCTATCATCCAGCCCTTACTCCCTGCTGTGCCTGCATCTCCACGGACCGGACGCCCATGGGCAGAACATCGTATGATCATCAATGGCATGTTCTGGGTCC
TTTGCTCAGGTGCACCATGGCGTGATTTACCTGAGCGATACGGCCTATGGAAGACCGTTTACAACCGCTTTAACAGATGGTCAAAGTCTGGACTGATTAA
CATCATTTTCAACAGGTTACTCTCACTACTTTATGCGAATGGCCTCGTTGACTGGTCTGCCACAGCGCTGGATGGCAGTAATATCCGGGCGCTGAAATGT
GCCGCCGGTGCTCAAAAAAACATCCCGATATTGCCGGAGATAATGGGCTGGGTCGCTCTCGAGGTGGTTTTGGCACCAAGATCCATCTGGCAACGGACGG
AAGCGGCCTCCCGTTAAATATCGTGCTGAGTCCCGGACAGGCTCATGAAAGCCAGTTCGCGCAACGACTCCTGGACGGCATTGGGGTTCAGCGTAAGAAC
GGCAGCATGAAACGCCGTGGGCATGCGGTACTGGCCGACAAAGCGTACTCCGGACATGTATTGCGCAACGAGCTGAAAAATAACGGCATAAAAGCAGTCA
TTCCCCGAAAATCAAATGAGAAAATGGCATCGGATGGACGTTCAAAGCTTGATCGTAATGCGTACCGCAATCGGAACGTCGTTGAGCGGTGTTTTGGGCG
TCTGAAAGAATACCGTCGCATCGCTACGCGTTACGACAAAACAGCGAGGAATTATCTGGCAATGGTGAAACTGGGCTGCATCCGCCTGTTTTACAGAAGG
TTATGCAATTAAGGGACACAGCCTAGTTG
GCTATCATCCAGCCCTTACTCCCTGCTGTGCCTGCATCTCCACGGACCGGACGCCCATGGGCAGAACATCGTATGATCATCAATGGCATGTTCTGGGTCC
TTTGCTCAGGTGCACCATGGCGTGATTTACCTGAGCGATACGGCCTATGGAAGACCGTTTACAACCGCTTTAACAGATGGTCAAAGTCTGGACTGATTAA
CATCATTTTCAACAGGTTACTCTCACTACTTTATGCGAATGGCCTCGTTGACTGGTCTGCCACAGCGCTGGATGGCAGTAATATCCGGGCGCTGAAATGT
GCCGCCGGTGCTCAAAAAAACATCCCGATATTGCCGGAGATAATGGGCTGGGTCGCTCTCGAGGTGGTTTTGGCACCAAGATCCATCTGGCAACGGACGG
AAGCGGCCTCCCGTTAAATATCGTGCTGAGTCCCGGACAGGCTCATGAAAGCCAGTTCGCGCAACGACTCCTGGACGGCATTGGGGTTCAGCGTAAGAAC
GGCAGCATGAAACGCCGTGGGCATGCGGTACTGGCCGACAAAGCGTACTCCGGACATGTATTGCGCAACGAGCTGAAAAATAACGGCATAAAAGCAGTCA
TTCCCCGAAAATCAAATGAGAAAATGGCATCGGATGGACGTTCAAAGCTTGATCGTAATGCGTACCGCAATCGGAACGTCGTTGAGCGGTGTTTTGGGCG
TCTGAAAGAATACCGTCGCATCGCTACGCGTTACGACAAAACAGCGAGGAATTATCTGGCAATGGTGAAACTGGGCTGCATCCGCCTGTTTTACAGAAGG
TTATGCAATTAAGGGACACAGCCTAGTTG
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 450 bp | 149 aa | 68 | 517 | + | No |
Description : First part of the transposase
ORF sequence :
MARYDIPDEAWAIIQPLLPAVPASPRTGRPWAEHRMIINGMFWVLCSGAPWRDLPERYGLWKTVYNRFNRWSKSGLINIIFNRLLSLLYANGLVDWSATA
LDGSNIRALKCAAGAQKNIPILPEIMGWVALEVVLAPRSIWQRTEAASR
LDGSNIRALKCAAGAQKNIPILPEIMGWVALEVVLAPRSIWQRTEAASR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 531 bp | 176 aa | 382 | 912 | + | No |
Description : Second part of the transposase
ORF sequence :
YPGAEMCRRCSKKHPDIAGDNGLGRSRGGFGTKIHLATDGSGLPLNIVLSPGQAHESQFAQRLLDGIGVQRKNGSMKRRGHAVLADKAYSGHVLRNELKN
NGIKAVIPRKSNEKMASDGRSKLDRNAYRNRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYRRLCN
NGIKAVIPRKSNEKMASDGRSKLDRNAYRNRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYRRLCN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 845 bp | 281 aa | 68 | 912 | + | Yes |
Chemistry : DDE
ORF sequence :
MARYDIPDEAWAIIQPLLPAVPASPRTGRPWAEHRMIINGMFWVLCSGAPWRDLPERYGLWKTVYNRFNRWSKSGLINIIFNRLLSLLYANGLVDWSATA
LDGSNIRALKCAAGAQKKHPDIAGDNGLGRSRGGFGTKIHLATDGSGLPLNIVLSPGQAHESQFAQRLLDGIGVQRKNGSMKRRGHAVLADKAYSGHVLR
NELKNNGIKAVIPRKSNEKMASDGRSKLDRNAYRNRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYRRLCN
LDGSNIRALKCAAGAQKKHPDIAGDNGLGRSRGGFGTKIHLATDGSGLPLNIVLSPGQAHESQFAQRLLDGIGVQRKNGSMKRRGHAVLADKAYSGHVLR
NELKNNGIKAVIPRKSNEKMASDGRSKLDRNAYRNRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYRRLCN
Blast result :
Comments
ISEhe2 is 88% and 95% aa similar to ISSfl7 ORF1 and ORF2 respectively.
pPATH is a plasmid of Erwinia herbicola pv gypsophilae.
pPATH is a plasmid of Erwinia herbicola pv gypsophilae.
References
1] Guo,M., Mor,H., Manulis,S. and Barash,I (2000) Direct submission GenBank.