ISLbp4
- Family IS4
- Group IS50
Isoform Synonym(s) ISLbp11
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| MVIT01000043 | ND | Leptospira kirschneri | Leptospira borgpetersenii serovar Hardjo-bovis L550 Leptospira kirschneri serovar Pomona M110/06 Leptospira borgpetersenii 200801926 |
DNA section
IS Length : 1484 bp
Ends
IR Length : 17/18
IRL : CCATAACCTTACCCACAAATATCATAAAAAATTAGATTTTAAAACCCAAT
IRR : TCATAACCTTACCCACAAGTCAGGCTTTTGTAAGTAATTTGAACATAAAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACGCGATCCG | TAGAGAG | CCATAACCTT | 7 |
| GAGCGACCCT | TAGGGAG | CGAGATGCTG | 7 |
DNA sequence
CCATAACCTTACCCACAAATATCATAAAAAATTAGATTTTAAAACCCAATCTTCCAAAATAGTTCAGGTGAATATAATGAACACTAAACATTGGAGTAGT
ACACTCGGAACGGAATTAGATTGGGTGGAAGAAGAATATCTGTCATTGAACTTAGGAGATAAAAGATTAGATAAACGACTAAAGAAAATAGTCAGCATGA
TGACCAAGCGAGGTGGAATGAGTTTGCCAGATATATTTGGCAATTGGTCAGGCACGAAGGGTGCATATCGTTTTTTTTCAAATCTGAAAGTATGTTATGA
TAAGATAATATCTCCTCATAGCCAAGCAACAAAGAACCGAATACAAAAACAAGAAACAGTATTGGTATTAAGCGACACAACGGAAATCTATTATAGAAAA
AGGGATCACGTTGAAGGCTTGGGACCGATGAACTCTGAATACAATCAGGGTCTTCTATTGCATCCATCGATAGCGTTTACGCCTGATGGAATTCCTTTAG
GAATATTGGACTTAAAAATGTGGTCGCGGACGGTATTAGGTGGAAATCGAAGTCAAGACGGAAGAAAGATGTCCATCGAAGATAAGGAAAGTGTAAAGTG
GATTCAAGGATACAGAGCCCTCTGTGAATTTGCAAAAGAATCCGATTCTAAATACGTATATATCTGCGATAGAGAAGCGGATATATACGAGCTATTCCAG
GAATACGTAGTTGCTGGCGAAAACGCTCCTGATATGCTTATACGCGCAAATCACGAAAGGAAGATAGAAGGTGGCGGTTGTTCTTGGTCCTATCTTGAAA
CTTTGGAACCTGCACATACATATACGATTACTGTACCAAGAAAAAAAGGAAAAGAAGCAAGGGAAGCAACCATTGAACTTCGATTTGAAAAGCTAACGAT
AAAATCCCCTCAATACAAGAAATTAGAAAATATTGATATGTATGCGTTAACCGCAACTGAAGTAGACGGACCCAAGGAAGAATCCATCAATTGGAAATTT
TTAACCACCATTCCTATTCACAATTCAGACGATGCAAAAAGAATGATCGCATATTACAAAAGCCGTTGGGGTATCGAGGTATTCTTCAAGGTTTTAAAGT
CGGGATGTAATATTGAATCGACTCAATTTAAATTTGGAGATCGATTCAAAGCTTGTATTGCAGTCTCTGCTATCGTTGCTTGGAGAGTTATGATGTTAAC
ATTCTTAGGTAGAAATATTCCAGGCTTAAAAGCGTCCATTATGTTCGAGTCTTTTGAATGGAAGGGCATTTATTGTAGGATTTTTGAAACTCCAAAACCA
CCTAACAAAGAACCAGATTTGGATTCCGTTTTGAGCTGGATCGCTAAATTAGGTGGACACTTGGATCGTAAATCCGACGCTCCCCCTGGACCGCTTGTAA
TTTTTAAAGGCCTTATGAGAGCTGTTGAAATCGGATTTATGTTCAAATTACTTACAAAAGCCTGACTTGTGGGTAAGGTTATGA
ACACTCGGAACGGAATTAGATTGGGTGGAAGAAGAATATCTGTCATTGAACTTAGGAGATAAAAGATTAGATAAACGACTAAAGAAAATAGTCAGCATGA
TGACCAAGCGAGGTGGAATGAGTTTGCCAGATATATTTGGCAATTGGTCAGGCACGAAGGGTGCATATCGTTTTTTTTCAAATCTGAAAGTATGTTATGA
TAAGATAATATCTCCTCATAGCCAAGCAACAAAGAACCGAATACAAAAACAAGAAACAGTATTGGTATTAAGCGACACAACGGAAATCTATTATAGAAAA
AGGGATCACGTTGAAGGCTTGGGACCGATGAACTCTGAATACAATCAGGGTCTTCTATTGCATCCATCGATAGCGTTTACGCCTGATGGAATTCCTTTAG
GAATATTGGACTTAAAAATGTGGTCGCGGACGGTATTAGGTGGAAATCGAAGTCAAGACGGAAGAAAGATGTCCATCGAAGATAAGGAAAGTGTAAAGTG
GATTCAAGGATACAGAGCCCTCTGTGAATTTGCAAAAGAATCCGATTCTAAATACGTATATATCTGCGATAGAGAAGCGGATATATACGAGCTATTCCAG
GAATACGTAGTTGCTGGCGAAAACGCTCCTGATATGCTTATACGCGCAAATCACGAAAGGAAGATAGAAGGTGGCGGTTGTTCTTGGTCCTATCTTGAAA
CTTTGGAACCTGCACATACATATACGATTACTGTACCAAGAAAAAAAGGAAAAGAAGCAAGGGAAGCAACCATTGAACTTCGATTTGAAAAGCTAACGAT
AAAATCCCCTCAATACAAGAAATTAGAAAATATTGATATGTATGCGTTAACCGCAACTGAAGTAGACGGACCCAAGGAAGAATCCATCAATTGGAAATTT
TTAACCACCATTCCTATTCACAATTCAGACGATGCAAAAAGAATGATCGCATATTACAAAAGCCGTTGGGGTATCGAGGTATTCTTCAAGGTTTTAAAGT
CGGGATGTAATATTGAATCGACTCAATTTAAATTTGGAGATCGATTCAAAGCTTGTATTGCAGTCTCTGCTATCGTTGCTTGGAGAGTTATGATGTTAAC
ATTCTTAGGTAGAAATATTCCAGGCTTAAAAGCGTCCATTATGTTCGAGTCTTTTGAATGGAAGGGCATTTATTGTAGGATTTTTGAAACTCCAAAACCA
CCTAACAAAGAACCAGATTTGGATTCCGTTTTGAGCTGGATCGCTAAATTAGGTGGACACTTGGATCGTAAATCCGACGCTCCCCCTGGACCGCTTGTAA
TTTTTAAAGGCCTTATGAGAGCTGTTGAAATCGGATTTATGTTCAAATTACTTACAAAAGCCTGACTTGTGGGTAAGGTTATGA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1389 bp | 462 aa | 77 | 1465 | + | No |
Chemistry : DDE
ORF sequence :
MNTKHWSSTLGTELDWVEEEYLSLNLGDKRLDKRLKKIVSMMTKRGGMSLPDIFGNWSGTKGAYRFFSNLKVCYDKIISPHSQATKNRIQKQETVLVLSD
TTEIYYRKRDHVEGLGPMNSEYNQGLLLHPSIAFTPDGIPLGILDLKMWSRTVLGGNRSQDGRKMSIEDKESVKWIQGYRALCEFAKESDSKYVYICDRE
ADIYELFQEYVVAGENAPDMLIRANHERKIEGGGCSWSYLETLEPAHTYTITVPRKKGKEAREATIELRFEKLTIKSPQYKKLENIDMYALTATEVDGPK
EESINWKFLTTIPIHNSDDAKRMIAYYKSRWGIEVFFKVLKSGCNIESTQFKFGDRFKACIAVSAIVAWRVMMLTFLGRNIPGLKASIMFESFEWKGIYC
RIFETPKPPNKEPDLDSVLSWIAKLGGHLDRKSDAPPGPLVIFKGLMRAVEIGFMFKLLTKA
TTEIYYRKRDHVEGLGPMNSEYNQGLLLHPSIAFTPDGIPLGILDLKMWSRTVLGGNRSQDGRKMSIEDKESVKWIQGYRALCEFAKESDSKYVYICDRE
ADIYELFQEYVVAGENAPDMLIRANHERKIEGGGCSWSYLETLEPAHTYTITVPRKKGKEAREATIELRFEKLTIKSPQYKKLENIDMYALTATEVDGPK
EESINWKFLTTIPIHNSDDAKRMIAYYKSRWGIEVFFKVLKSGCNIESTQFKFGDRFKACIAVSAIVAWRVMMLTFLGRNIPGLKASIMFESFEWKGIYC
RIFETPKPPNKEPDLDSVLSWIAKLGGHLDRKSDAPPGPLVIFKGLMRAVEIGFMFKLLTKA
Blast result :
Comments
ISLbp4 is 56% aa similar to ISLpn6.
In the genome of Leptospira borgpetersenii serovar Hardjo-bovis L550 (CP000348), the authors use two IS names to designate this IS : ISLbp4 for the Nter and ISLbp11 for the Cter of the transposase. In this genome, the sequence is mutated (unexpected frameshift).
In the genome of Leptospira borgpetersenii serovar Hardjo-bovis L550 (CP000348), the authors use two IS names to designate this IS : ISLbp4 for the Nter and ISLbp11 for the Cter of the transposase. In this genome, the sequence is mutated (unexpected frameshift).
References
1] ISfinder submission (2018)
2] Bulach,D.M., Zuerner,R.L., Wilson,P., Seemann,T., McGrath,A., Cullen,P.A., Davis,J., Johnson,M., Kuczek,E., Alt,D.P., Peterson-Burch,B., Coppel,R.L., Rood,J.I., Davies,J.K. and Adler,B. (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (39), 14560-14565 .
3] Moreno,L.Z., Miraglia,F., Kremer,F.S., Eslabao,M.R., Lilenbaum,W., Dellagostin,O.A. and Moreno,A.M. (2017) Direct GenBank submission.
2] Bulach,D.M., Zuerner,R.L., Wilson,P., Seemann,T., McGrath,A., Cullen,P.A., Davis,J., Johnson,M., Kuczek,E., Alt,D.P., Peterson-Burch,B., Coppel,R.L., Rood,J.I., Davies,J.K. and Adler,B. (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (39), 14560-14565 .
3] Moreno,L.Z., Miraglia,F., Kremer,F.S., Eslabao,M.R., Lilenbaum,W., Dellagostin,O.A. and Moreno,A.M. (2017) Direct GenBank submission.