ISLpl2
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP024414 | ND | Lactobacillus plantarum | Lactobacillus plantarum ATCC 8014 pLP49 Lactobacillus plantarum CCM 1904 |
DNA section
IS Length : 1491 bp
Ends
IR Length : 24/27
IRL : TGAAGTGAACCCCCGAGATTGGACAACAATCTCGGGGGTTTTACTATGAC
IRR : TGAAGTGAACCCACAAGTTTGGACAACTAAATTAAGCTGCTAACTGATTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAAGCCACCC | ACC | TCGTGATGAG | 3 |
DNA sequence
TGAAGTGAACCCCCGAGATTGGACAACAATCTCGGGGGTTTTACTATGACTAAATTTAATTTAGAGTTACGGATTTCTATTGTGACACAATATTTAACGG
GTATTAGTTCCATTCAATTGGCCAGAAAATACCATGTTGCCAACAATGAAACGATTCTCCTATGGGTTCATCGCTTTAATCGTTTTGGCATTGCAGGATT
AAAACCGAAACCTATGAATTTGGAATACTCTAGTGAGTTCAAGATAGAAGTATTAAACTGGAAACAACAGCACAAAGCTTCGCTTCCAGAAACAGCACTA
CATTTCAACCTCTCTTCACCAAGTACTATCTGGCAATGGCAAAGAAAGTTTGATTTAGAGGGGCTCAGTAGACTAAATAGAGTGAGAGGAAATCCAAAGA
CTATGTCTAAACATAAAAAAGTGACCAAACCCACAACGGCACAAATCCAAGCACGCCACGATAAAGATGAGTTAAAGCAACTCAAGCAAGAAAATAAGAT
GTTAAGAATCGAGAACGAATTTTTAAAAAAACTCGATGCCTTAGATCACGAAAAATCAGCTCACGAGAAACCGTGACCTTAATTGATGATCTGAGGCGGG
TCTTCAAAACGTCGATTAGTTTTATCCTTAAAGCCGTTAAGGTACCACGTAGCACGTACTATTACACTAAGCACAGTCAGGGACGAAAATATGAGGACGA
CCAGGTTATCCAAGCCATTGATGAAATTCGGCAAACAGATGCCAAGTATACTCAAAAATACGGTTACCGTCGCATAACGTTAGTTATGCATGAACAGGGA
TTTAAAGTTAATCATAAACGCGTCCTCCGAATTATGAAGGAGCAAGGCTGGACATGTCAGGCCTTTAATAAGCAAACTCGCAAATATAACTCATACAAAG
GAACTATTGGTAGAATCGCCAAAAATAAGTTACACCGTCGATTCAAGACAGATCGACCATATCAAAAACTCGTCGCCGACGTTAGTGAATTTCGATACGG
TCAGATGAGTCAAAGCGAGCGAATCTATTTAGAACCCATCATGGATTTATTTTCGGGTGAAATTTTGGCATTTAACATTAGCGCACACCCAACTCTTGAA
TTTGCGTTGAAACCACTAAAAGAAGCATTAGATGGATTACCTGAGCTTCCTTATAGAACAACGGTACACACAGATCAAGGATTTCAGTATCAACATAAAC
AGTGGCAAAAAACACTTAAAACTCATCATACCTTTCAGAGTATGTCCCGTAAGGCGACTTGCTTAGATAACGCCGCCATGGAATCATTTTTTCATCTCAT
GAAAGCAGAGATGATGGATGAACACTTCGAAAATTCAGAAAGTCTCGCGCAAGCGATGACTGAATGGATTGAATTTTATAATAACCGTAGAATCAGGACC
AAACTAAAAGGCAAGTCACCGGTACAATACCGAGAACTTGCCAATCAGTTAGCAGCTTAATTTAGTTGTCCAAACTTGTGGGTTCACTTCA
GTATTAGTTCCATTCAATTGGCCAGAAAATACCATGTTGCCAACAATGAAACGATTCTCCTATGGGTTCATCGCTTTAATCGTTTTGGCATTGCAGGATT
AAAACCGAAACCTATGAATTTGGAATACTCTAGTGAGTTCAAGATAGAAGTATTAAACTGGAAACAACAGCACAAAGCTTCGCTTCCAGAAACAGCACTA
CATTTCAACCTCTCTTCACCAAGTACTATCTGGCAATGGCAAAGAAAGTTTGATTTAGAGGGGCTCAGTAGACTAAATAGAGTGAGAGGAAATCCAAAGA
CTATGTCTAAACATAAAAAAGTGACCAAACCCACAACGGCACAAATCCAAGCACGCCACGATAAAGATGAGTTAAAGCAACTCAAGCAAGAAAATAAGAT
GTTAAGAATCGAGAACGAATTTTTAAAAAAACTCGATGCCTTAGATCACGAAAAATCAGCTCACGAGAAACCGTGACCTTAATTGATGATCTGAGGCGGG
TCTTCAAAACGTCGATTAGTTTTATCCTTAAAGCCGTTAAGGTACCACGTAGCACGTACTATTACACTAAGCACAGTCAGGGACGAAAATATGAGGACGA
CCAGGTTATCCAAGCCATTGATGAAATTCGGCAAACAGATGCCAAGTATACTCAAAAATACGGTTACCGTCGCATAACGTTAGTTATGCATGAACAGGGA
TTTAAAGTTAATCATAAACGCGTCCTCCGAATTATGAAGGAGCAAGGCTGGACATGTCAGGCCTTTAATAAGCAAACTCGCAAATATAACTCATACAAAG
GAACTATTGGTAGAATCGCCAAAAATAAGTTACACCGTCGATTCAAGACAGATCGACCATATCAAAAACTCGTCGCCGACGTTAGTGAATTTCGATACGG
TCAGATGAGTCAAAGCGAGCGAATCTATTTAGAACCCATCATGGATTTATTTTCGGGTGAAATTTTGGCATTTAACATTAGCGCACACCCAACTCTTGAA
TTTGCGTTGAAACCACTAAAAGAAGCATTAGATGGATTACCTGAGCTTCCTTATAGAACAACGGTACACACAGATCAAGGATTTCAGTATCAACATAAAC
AGTGGCAAAAAACACTTAAAACTCATCATACCTTTCAGAGTATGTCCCGTAAGGCGACTTGCTTAGATAACGCCGCCATGGAATCATTTTTTCATCTCAT
GAAAGCAGAGATGATGGATGAACACTTCGAAAATTCAGAAAGTCTCGCGCAAGCGATGACTGAATGGATTGAATTTTATAATAACCGTAGAATCAGGACC
AAACTAAAAGGCAAGTCACCGGTACAATACCGAGAACTTGCCAATCAGTTAGCAGCTTAATTTAGTTGTCCAAACTTGTGGGTTCACTTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 531 bp | 176 aa | 46 | 576 | + | No |
Description : First part of the transposase
ORF sequence :
MTKFNLELRISIVTQYLTGISSIQLARKYHVANNETILLWVHRFNRFGIAGLKPKPMNLEYSSEFKIEVLNWKQQHKASLPETALHFNLSSPSTIWQWQR
KFDLEGLSRLNRVRGNPKTMSKHKKVTKPTTAQIQARHDKDELKQLKQENKMLRIENEFLKKLDALDHEKSAHEKP
KFDLEGLSRLNRVRGNPKTMSKHKKVTKPTTAQIQARHDKDELKQLKQENKMLRIENEFLKKLDALDHEKSAHEKP
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 963 bp | 320 aa | 498 | 1460 | + | No |
Description : Second part of the transposase
ORF sequence :
DVKNRERIFKKTRCLRSRKISSRETVTLIDDLRRVFKTSISFILKAVKVPRSTYYYTKHSQGRKYEDDQVIQAIDEIRQTDAKYTQKYGYRRITLVMHEQ
GFKVNHKRVLRIMKEQGWTCQAFNKQTRKYNSYKGTIGRIAKNKLHRRFKTDRPYQKLVADVSEFRYGQMSQSERIYLEPIMDLFSGEILAFNISAHPTL
EFALKPLKEALDGLPELPYRTTVHTDQGFQYQHKQWQKTLKTHHTFQSMSRKATCLDNAAMESFFHLMKAEMMDEHFENSESLAQAMTEWIEFYNNRRIR
TKLKGKSPVQYRELANQLAA
GFKVNHKRVLRIMKEQGWTCQAFNKQTRKYNSYKGTIGRIAKNKLHRRFKTDRPYQKLVADVSEFRYGQMSQSERIYLEPIMDLFSGEILAFNISAHPTL
EFALKPLKEALDGLPELPYRTTVHTDQGFQYQHKQWQKTLKTHHTFQSMSRKATCLDNAAMESFFHLMKAEMMDEHFENSESLAQAMTEWIEFYNNRRIR
TKLKGKSPVQYRELANQLAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1415 bp | 471 aa | 46 | 1460 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKFNLELRISIVTQYLTGISSIQLARKYHVANNETILLWVHRFNRFGIAGLKPKPMNLEYSSEFKIEVLNWKQQHKASLPETALHFNLSSPSTIWQWQR
KFDLEGLSRLNRVRGNPKTMSKHKKVTKPTTAQIQARHDKDELKQLKQENKMLRIENEFLKKTRCLRSRKISSRETVTLIDDLRRVFKTSISFILKAVKV
PRSTYYYTKHSQGRKYEDDQVIQAIDEIRQTDAKYTQKYGYRRITLVMHEQGFKVNHKRVLRIMKEQGWTCQAFNKQTRKYNSYKGTIGRIAKNKLHRRF
KTDRPYQKLVADVSEFRYGQMSQSERIYLEPIMDLFSGEILAFNISAHPTLEFALKPLKEALDGLPELPYRTTVHTDQGFQYQHKQWQKTLKTHHTFQSM
SRKATCLDNAAMESFFHLMKAEMMDEHFENSESLAQAMTEWIEFYNNRRIRTKLKGKSPVQYRELANQLAA
KFDLEGLSRLNRVRGNPKTMSKHKKVTKPTTAQIQARHDKDELKQLKQENKMLRIENEFLKKTRCLRSRKISSRETVTLIDDLRRVFKTSISFILKAVKV
PRSTYYYTKHSQGRKYEDDQVIQAIDEIRQTDAKYTQKYGYRRITLVMHEQGFKVNHKRVLRIMKEQGWTCQAFNKQTRKYNSYKGTIGRIAKNKLHRRF
KTDRPYQKLVADVSEFRYGQMSQSERIYLEPIMDLFSGEILAFNISAHPTLEFALKPLKEALDGLPELPYRTTVHTDQGFQYQHKQWQKTLKTHHTFQSM
SRKATCLDNAAMESFFHLMKAEMMDEHFENSESLAQAMTEWIEFYNNRRIRTKLKGKSPVQYRELANQLAA
Blast result :
Comments
ISLpl2 is 61% aa similar to ISLsa2.
The third ORF is the putative ORFAB reconstructed in silico by possible -1 frameshift.
In the file (Accession number : AF459445) the ISLpl2 is truncated.
The third ORF is the putative ORFAB reconstructed in silico by possible -1 frameshift.
In the file (Accession number : AF459445) the ISLpl2 is truncated.
References
1] ISfinder submission (2018)
2] Nicoloff,H. and Bringel,F. (2003) Appl. Environ. Microbiol. 69 (10), 6032-6040.
3] Muhammad,S., Zheng,J. and Sun,M. (2017) Direct GenBank submission.
2] Nicoloff,H. and Bringel,F. (2003) Appl. Environ. Microbiol. 69 (10), 6032-6040.
3] Muhammad,S., Zheng,J. and Sun,M. (2017) Direct GenBank submission.