ISLhe30
- Family IS30
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CBUL010000181 | ND | Lactobacillus helveticus | Lactobacillus helveticus CIRM-BIA 104 |
DNA section
IS Length : 1025 bp
Ends
IR Length : 23/34
IRL : TGGACAGAAAAACCATCAAGGTCTTTAATGGTGTTACCGCACAATCCATT
IRR : TGGCAGAAATAACTTGAACGAATTTAGTGACGTAGATTAAGCAAGAAGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTTGAACATTTAAG | CAACTGGAAGAATA | 0 |
DNA sequence
TGGACAGAAAAACCATCAAGGTCTTTAATGGTGTTACCGCACAATCCATTAGAAAGAAGGACCTTGATGAGCACCACTATTTTATCATTCCAGAACCGTG
TTGTCATTGAAACGCTTCATAATGAAGGACGTTATGAAGGACGTTCCTTGCGATACATCGCTAACTACTTAGGCTTTAGTAAGACCACCATCTTTAACGA
ACTTCACCGGCTAAATAGTGAGTACCAGACTGGGCTAGCGCAAACTGACTTTGAACGAAAGGTTAGTCAACGGGGGCGGAAGTCTTCGCTCACTAAAAAC
CTTAAACACTTGATCGAGGAAAAGATTCAAGTCCAGAAGTGGTCCCCTGAACAAGTTGCCCATGTGGTTGGGATTGCCTACAAGACGGTCTATAACTGGA
TTGATCAAGGCTGGCTTGATATACAGTTGCCCGATTTGCCTGATCATGGAATTCGTCGTCATCGTGCTAAAGAAAAGCGTGGTACATTCAATCACGGCTG
CTCAATTGAGGAGAGTCCTCATAAAGTCGAAACTCGCCAGGAATTCGGCTACTTTGAAGCTGATACCGTATTTTCTGGCAAACGTAAAGGTCAAGCTGTG
GCTACTTTTGTGGAGCGTAAGAGTCGCCTGACAATTGTTAAACGGCTCCATGGTCGCGACAGTCAGTCCATGACTCAAGCCGTACTTGAACTAGCTAGTC
AACTTCAAGACAAGCTCAAGACGCTTACCGTGGATCATGGTAAAGAGTTCGCTAACTATCAGACAATTGAACGGCAAACTGGTACTCAGGTTTATTTTGC
CCATGCTTATTCACCACATGAACGCGGTAGTAATGAGAACCGTAACCGAGTTTTACGACGATTTATTCCTAAAGGCCAAGCCATTGAAGAGTTAAGCGAT
CACAAGCTGATTCAAATTAATTGGTATTTGAATTCCCGGCCACTTAAATGTCTTAATTGGCATACACCAATCGAGAGCTTCTTGCTTAATCTACGTCACT
AAATTCGTTCAAGTTATTTCTGCCA
TTGTCATTGAAACGCTTCATAATGAAGGACGTTATGAAGGACGTTCCTTGCGATACATCGCTAACTACTTAGGCTTTAGTAAGACCACCATCTTTAACGA
ACTTCACCGGCTAAATAGTGAGTACCAGACTGGGCTAGCGCAAACTGACTTTGAACGAAAGGTTAGTCAACGGGGGCGGAAGTCTTCGCTCACTAAAAAC
CTTAAACACTTGATCGAGGAAAAGATTCAAGTCCAGAAGTGGTCCCCTGAACAAGTTGCCCATGTGGTTGGGATTGCCTACAAGACGGTCTATAACTGGA
TTGATCAAGGCTGGCTTGATATACAGTTGCCCGATTTGCCTGATCATGGAATTCGTCGTCATCGTGCTAAAGAAAAGCGTGGTACATTCAATCACGGCTG
CTCAATTGAGGAGAGTCCTCATAAAGTCGAAACTCGCCAGGAATTCGGCTACTTTGAAGCTGATACCGTATTTTCTGGCAAACGTAAAGGTCAAGCTGTG
GCTACTTTTGTGGAGCGTAAGAGTCGCCTGACAATTGTTAAACGGCTCCATGGTCGCGACAGTCAGTCCATGACTCAAGCCGTACTTGAACTAGCTAGTC
AACTTCAAGACAAGCTCAAGACGCTTACCGTGGATCATGGTAAAGAGTTCGCTAACTATCAGACAATTGAACGGCAAACTGGTACTCAGGTTTATTTTGC
CCATGCTTATTCACCACATGAACGCGGTAGTAATGAGAACCGTAACCGAGTTTTACGACGATTTATTCCTAAAGGCCAAGCCATTGAAGAGTTAAGCGAT
CACAAGCTGATTCAAATTAATTGGTATTTGAATTCCCGGCCACTTAAATGTCTTAATTGGCATACACCAATCGAGAGCTTCTTGCTTAATCTACGTCACT
AAATTCGTTCAAGTTATTTCTGCCA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 936 bp | 311 aa | 67 | 1002 | + | No |
Chemistry : DDE
ORF sequence :
MSTTILSFQNRVVIETLHNEGRYEGRSLRYIANYLGFSKTTIFNELHRLNSEYQTGLAQTDFERKVSQRGRKSSLTKNLKHLIEEKIQVQKWSPEQVAHV
VGIAYKTVYNWIDQGWLDIQLPDLPDHGIRRHRAKEKRGTFNHGCSIEESPHKVETRQEFGYFEADTVFSGKRKGQAVATFVERKSRLTIVKRLHGRDSQ
SMTQAVLELASQLQDKLKTLTVDHGKEFANYQTIERQTGTQVYFAHAYSPHERGSNENRNRVLRRFIPKGQAIEELSDHKLIQINWYLNSRPLKCLNWHT
PIESFLLNLRH
VGIAYKTVYNWIDQGWLDIQLPDLPDHGIRRHRAKEKRGTFNHGCSIEESPHKVETRQEFGYFEADTVFSGKRKGQAVATFVERKSRLTIVKRLHGRDSQ
SMTQAVLELASQLQDKLKTLTVDHGKEFANYQTIERQTGTQVYFAHAYSPHERGSNENRNRVLRRFIPKGQAIEELSDHKLIQINWYLNSRPLKCLNWHT
PIESFLLNLRH
Blast result :
Comments
ISLhe30 is 64% aa similar to IS1070.
The original sequence named ISLhe30 in Lactobacillus helveticus DPC 4571 (CP000517) share unexpected frameshift. The submitted sequence is the closely related non-mutated sequence (98% aa similar).
The original sequence named ISLhe30 in Lactobacillus helveticus DPC 4571 (CP000517) share unexpected frameshift. The submitted sequence is the closely related non-mutated sequence (98% aa similar).
References
1] ISfinder submission (2018)
2] Valence,F., Chuat,V., Ma,L., Creno,S., Falentin,H., Lortal,S., Bizet,C., Clermont,D., Loux,V., Bouchier,C. and Cousin,S. (2013) Direct GenBank submission.
2] Valence,F., Chuat,V., Ma,L., Creno,S., Falentin,H., Lortal,S., Bizet,C., Clermont,D., Loux,V., Bouchier,C. and Cousin,S. (2013) Direct GenBank submission.