ISFw4
- Family IS30
- Group
Isoform Synonym(s) ISFw5
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP010427 | ND | Allofrancisella guangzhouensis | Francisella sp. W12-1067 Allofrancisella guangzhouensis 08HL01032 |
DNA section
IS Length : 1046 bp
Ends
IR Length : 20/23
IRL : GCTAGATTGTATTCTGAAAGTTTTAAAATAGTTGAAAGAGAGTTCCAGAT
IRR : GCTAGATTGTAGTCTCAATGTTTATACTGATTCCGAAATGTACCTCATTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATGTTTTATA | TAA | TAAAAACTAT | 3 |
DNA sequence
GCTAGATTGTATTCTGAAAGTTTTAAAATAGTTGAAAGAGAGTTCCAGATGTAGTTTATTAGGTTTGAGAAGAACTTAAATAAACGAACAGGAACTAAAA
ATGGCACATAGACATTTAACTCTTTCAGATAGATATTATATAGAAAATTTACTTAAATTAGGATACTTAGAAGCAGCAATAGCTAATAAAATAGGATTTA
GTAAAACAGCTGTTATAAATGAACTTAAAAGGAATAAAGTAGGTAAAAGTTATTCTGCAGAGATAGCCCATAAGTTGTATTGTAGTCGTAGAAAGTCAAA
TAATCATAAACTTACTAATGAAGTTAAAAAGCTGATAATAGCTTTACTCAAAAAGAAAATATCACCGGAGTTAATTTGTGGTAGATTAAAGCATGAAGGT
ATAGCTAAGCTAAGTTTTAAAGCTGTTTATAACTTTATACAAAGACATAACCTTAAACAGTTATTATTCTTCAAAGGTAGGCGTTACAAATACAAAAAAG
AAGGCTCCTCAAATCAAGGTAAGATAAAGGATAGAGTCAACATATCACAAAGGCCTAAAGCAGCTAATGATAGAAAAGAGCTGTATCATTTTGAAGGTGA
TACTATAGTCGGTAAAGATCATAAAGGAGCTATTTTGACTATGGTAGATAGAGTTAGTAGATTCACAATTCTAGGCAAAGCTAAAGATAGAACAGCTAGT
TCAATTAACCAAATTTTATACAGAGCATCAAATGGTAATAAAATTACCACAGCTACTTTTGATAATGGTAAAGAGTTTGCTAAACACAAAAATTTATCAA
ATAAAACTGGTATTAAAGTATTCTTTGCAGATGCATATAGTCCATGGCAAAGAGGTACAAATGAAAACATGAATAGGTATATCAGACAATTTATTCCTAA
AGGTACTGATTTTAGTAATATCTCTCATCAGTATCTTAGAAAGTTGCAAATTGACTTGAATAATAGACCTAAAAAATGTTTAAATTATTTAACACCTAAT
GAGGTACATTTCGGAATCAGTATAAACATTGAGACTACAATCTAGC
ATGGCACATAGACATTTAACTCTTTCAGATAGATATTATATAGAAAATTTACTTAAATTAGGATACTTAGAAGCAGCAATAGCTAATAAAATAGGATTTA
GTAAAACAGCTGTTATAAATGAACTTAAAAGGAATAAAGTAGGTAAAAGTTATTCTGCAGAGATAGCCCATAAGTTGTATTGTAGTCGTAGAAAGTCAAA
TAATCATAAACTTACTAATGAAGTTAAAAAGCTGATAATAGCTTTACTCAAAAAGAAAATATCACCGGAGTTAATTTGTGGTAGATTAAAGCATGAAGGT
ATAGCTAAGCTAAGTTTTAAAGCTGTTTATAACTTTATACAAAGACATAACCTTAAACAGTTATTATTCTTCAAAGGTAGGCGTTACAAATACAAAAAAG
AAGGCTCCTCAAATCAAGGTAAGATAAAGGATAGAGTCAACATATCACAAAGGCCTAAAGCAGCTAATGATAGAAAAGAGCTGTATCATTTTGAAGGTGA
TACTATAGTCGGTAAAGATCATAAAGGAGCTATTTTGACTATGGTAGATAGAGTTAGTAGATTCACAATTCTAGGCAAAGCTAAAGATAGAACAGCTAGT
TCAATTAACCAAATTTTATACAGAGCATCAAATGGTAATAAAATTACCACAGCTACTTTTGATAATGGTAAAGAGTTTGCTAAACACAAAAATTTATCAA
ATAAAACTGGTATTAAAGTATTCTTTGCAGATGCATATAGTCCATGGCAAAGAGGTACAAATGAAAACATGAATAGGTATATCAGACAATTTATTCCTAA
AGGTACTGATTTTAGTAATATCTCTCATCAGTATCTTAGAAAGTTGCAAATTGACTTGAATAATAGACCTAAAAAATGTTTAAATTATTTAACACCTAAT
GAGGTACATTTCGGAATCAGTATAAACATTGAGACTACAATCTAGC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 945 bp | 314 aa | 101 | 1045 | + | No |
Chemistry : DDE
ORF sequence :
MAHRHLTLSDRYYIENLLKLGYLEAAIANKIGFSKTAVINELKRNKVGKSYSAEIAHKLYCSRRKSNNHKLTNEVKKLIIALLKKKISPELICGRLKHEG
IAKLSFKAVYNFIQRHNLKQLLFFKGRRYKYKKEGSSNQGKIKDRVNISQRPKAANDRKELYHFEGDTIVGKDHKGAILTMVDRVSRFTILGKAKDRTAS
SINQILYRASNGNKITTATFDNGKEFAKHKNLSNKTGIKVFFADAYSPWQRGTNENMNRYIRQFIPKGTDFSNISHQYLRKLQIDLNNRPKKCLNYLTPN
EVHFGISINIETTI
IAKLSFKAVYNFIQRHNLKQLLFFKGRRYKYKKEGSSNQGKIKDRVNISQRPKAANDRKELYHFEGDTIVGKDHKGAILTMVDRVSRFTILGKAKDRTAS
SINQILYRASNGNKITTATFDNGKEFAKHKNLSNKTGIKVFFADAYSPWQRGTNENMNRYIRQFIPKGTDFSNISHQYLRKLQIDLNNRPKKCLNYLTPN
EVHFGISINIETTI
Blast result :
Comments
ISFw4 is 58% aa similar to IS4351.
The first copy named ISFw4 in Francisella sp. W12-1067 is truncated.
The first copy named ISFw4 in Francisella sp. W12-1067 is truncated.
References
1] ISfinder soumission (2018)
2] Rydzewski,K., Schulz,T., Brzuszkiewicz,E., Holland,G., Luck,C., Fleischer,J., Grunow,R. and Heuner,K. (2014) BMC Microbiol. 14 (1), 169.
3] Svensson,D., Ohrman,C., Backman,S., Karlsson,E., Nilsson,E., Bystrom,M., Larkeryd,A., Stenberg,P., Scholtz,H.C., Forsman,M. and Sjodin,A. (2014) Direct GenBank submission.
2] Rydzewski,K., Schulz,T., Brzuszkiewicz,E., Holland,G., Luck,C., Fleischer,J., Grunow,R. and Heuner,K. (2014) BMC Microbiol. 14 (1), 169.
3] Svensson,D., Ohrman,C., Backman,S., Karlsson,E., Nilsson,E., Bystrom,M., Larkeryd,A., Stenberg,P., Scholtz,H.C., Forsman,M. and Sjodin,A. (2014) Direct GenBank submission.