ISEch13
- Family IS110
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP002038 | ND | Dickeya dadantii | Dickeya dadantii 3937 |
DNA section
IS Length : 1525 bp
Ends
Left end : GGTCCGCTCAGTGCCAGATATAGTCATTCACACCCTGAGAATTACACTTTTAAGTGATTCAGGGTATGCGCTTACTGGTTACGAATATTGTGCCAGAATC
Right end : TACGACGAAGCGAAATATTTGCTGGCACTGGAAGAACGAAAATCGTCCTTACTTATGCCATAAAAAACTTGTCGAATGCCTCAGGGCGTGAAGCAGACAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCTTCGGGCC | ATTTATTTAA | 0 |
DNA sequence
GGTCCGCTCAGTGCCAGATATAGTCATTCACACCCTGAGAATTACACTTTTAAGTGATTCAGGGTATGCGCTTACTGGTTACGAATATTGTGCCAGAATC
ATGTGACTTGATGATTGCCGCTTTGCTTTCGAGCAAGTAAGTGCTCCACATTCGCTAACGAATACTCAGGGCATGCAGAAAAACTGCTGACTATATTTCT
TTCGAAGAACGTGGAATGCATACCAATCCCCGATAAAAAGGAGTTGGTGATGGCTGCGATAGATCAGTTTGCTGCTCATGTTGGTCTGGACTGGGCGGAT
AAAAAGCATGATGTCTGCGTTCAGTTTAAAAACGGCGAACGCATATTCCATGTGATTGAACATACTCCGGAAGCGCTTGATATCTGGCTCAACGAGTTGC
ACCAAAAGGTGAAAGGCAGGATCGCTATCGCTCTTGAGCTGAAGAAAGGCCCGGTGGTGTATGCTCTGCAGAAGTACCCATTCGTCACTGTCTTTCCTGT
ACACGCGTTGTCACTGGCCCGCTATCGGCAGACCTTCTGGCCAAGTGGTGCGAAGGATGATCCCCAGGATGCTGAGTTAGCATTAGAGCTAATGTTACGC
TATCCCCAAAAGATAAAGGCCATAGAACCCGACAATGCTGATATCCGGTTACTTCAACAATTAGTTGAGCAGCGTCGACTGCTGGTCGAGGACAAACGCC
GCTTTGTTAATCGACTCATCAACACGCTTAAGCAGTATTTTCCTCAGCCCCTGGAATGGTTCTCGCATCGGGACAGCTCACTGTTCTGCGAGCTAATAAT
CCGCTGGCCCAGTCTGCAACAGCTAAAACGTGCCCGGAGTGATACCGTCCGTCATTTTATGAACGCAAAAGGTGGCCCCGCTGTTGCGTATACAGAACAG
CGGGTTGCAAGTATTGCCAGCGCTATTCCCCTGACCACTGATAAAACTGTCATCGAGGCAAATGCATTGATGGCAACAGCACTTGCATCCCAAATCAAAC
TGGTAGGCGACCTCATCAGAACCTACGACGAGCGTATTGAATCCTTATTCGATACGCTTCCGGATGCAGAACTGTTCAAATCACTGCCAGGAATGGGGCC
GTGTATGGGACCGCGTATGCTTGCCGCACTCGGTGATAACCGCAACCGCTTCAATAGCGCCGAAGAAATTCAAAACTACGCTGGCATCGCGCCAGTGACA
GAGCGGAGTGGGCAAAAATCCTGGGTACACTGGCGTTGGCAGTGTGCGAAGTTCGTCCGACAGACATTCGTGGAATGGACTGCGAAGACGGTTAACGCAT
CATACTGGGCCAAACTTTATTACCAGGGCCAGCGAGAAAAAGGGAAATCACATCAATCAGCTATCCGGGCTCTGGCGTTCAAATGGATAAGGATCATTTA
CCGCTGTTGGAAGACCAAAACCCGGTACGACGAAGCGAAATATTTGCTGGCACTGGAAGAACGAAAATCGTCCTTACTTATGCCATAAAAAACTTGTCGA
ATGCCTCAGGGCGTGAAGCAGACAT
ATGTGACTTGATGATTGCCGCTTTGCTTTCGAGCAAGTAAGTGCTCCACATTCGCTAACGAATACTCAGGGCATGCAGAAAAACTGCTGACTATATTTCT
TTCGAAGAACGTGGAATGCATACCAATCCCCGATAAAAAGGAGTTGGTGATGGCTGCGATAGATCAGTTTGCTGCTCATGTTGGTCTGGACTGGGCGGAT
AAAAAGCATGATGTCTGCGTTCAGTTTAAAAACGGCGAACGCATATTCCATGTGATTGAACATACTCCGGAAGCGCTTGATATCTGGCTCAACGAGTTGC
ACCAAAAGGTGAAAGGCAGGATCGCTATCGCTCTTGAGCTGAAGAAAGGCCCGGTGGTGTATGCTCTGCAGAAGTACCCATTCGTCACTGTCTTTCCTGT
ACACGCGTTGTCACTGGCCCGCTATCGGCAGACCTTCTGGCCAAGTGGTGCGAAGGATGATCCCCAGGATGCTGAGTTAGCATTAGAGCTAATGTTACGC
TATCCCCAAAAGATAAAGGCCATAGAACCCGACAATGCTGATATCCGGTTACTTCAACAATTAGTTGAGCAGCGTCGACTGCTGGTCGAGGACAAACGCC
GCTTTGTTAATCGACTCATCAACACGCTTAAGCAGTATTTTCCTCAGCCCCTGGAATGGTTCTCGCATCGGGACAGCTCACTGTTCTGCGAGCTAATAAT
CCGCTGGCCCAGTCTGCAACAGCTAAAACGTGCCCGGAGTGATACCGTCCGTCATTTTATGAACGCAAAAGGTGGCCCCGCTGTTGCGTATACAGAACAG
CGGGTTGCAAGTATTGCCAGCGCTATTCCCCTGACCACTGATAAAACTGTCATCGAGGCAAATGCATTGATGGCAACAGCACTTGCATCCCAAATCAAAC
TGGTAGGCGACCTCATCAGAACCTACGACGAGCGTATTGAATCCTTATTCGATACGCTTCCGGATGCAGAACTGTTCAAATCACTGCCAGGAATGGGGCC
GTGTATGGGACCGCGTATGCTTGCCGCACTCGGTGATAACCGCAACCGCTTCAATAGCGCCGAAGAAATTCAAAACTACGCTGGCATCGCGCCAGTGACA
GAGCGGAGTGGGCAAAAATCCTGGGTACACTGGCGTTGGCAGTGTGCGAAGTTCGTCCGACAGACATTCGTGGAATGGACTGCGAAGACGGTTAACGCAT
CATACTGGGCCAAACTTTATTACCAGGGCCAGCGAGAAAAAGGGAAATCACATCAATCAGCTATCCGGGCTCTGGCGTTCAAATGGATAAGGATCATTTA
CCGCTGTTGGAAGACCAAAACCCGGTACGACGAAGCGAAATATTTGCTGGCACTGGAAGAACGAAAATCGTCCTTACTTATGCCATAAAAAACTTGTCGA
ATGCCTCAGGGCGTGAAGCAGACAT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1239 bp | 412 aa | 250 | 1488 | + | No |
Chemistry : DEDD
ORF sequence :
MAAIDQFAAHVGLDWADKKHDVCVQFKNGERIFHVIEHTPEALDIWLNELHQKVKGRIAIALELKKGPVVYALQKYPFVTVFPVHALSLARYRQTFWPSG
AKDDPQDAELALELMLRYPQKIKAIEPDNADIRLLQQLVEQRRLLVEDKRRFVNRLINTLKQYFPQPLEWFSHRDSSLFCELIIRWPSLQQLKRARSDTV
RHFMNAKGGPAVAYTEQRVASIASAIPLTTDKTVIEANALMATALASQIKLVGDLIRTYDERIESLFDTLPDAELFKSLPGMGPCMGPRMLAALGDNRNR
FNSAEEIQNYAGIAPVTERSGQKSWVHWRWQCAKFVRQTFVEWTAKTVNASYWAKLYYQGQREKGKSHQSAIRALAFKWIRIIYRCWKTKTRYDEAKYLL
ALEERKSSLLMP
AKDDPQDAELALELMLRYPQKIKAIEPDNADIRLLQQLVEQRRLLVEDKRRFVNRLINTLKQYFPQPLEWFSHRDSSLFCELIIRWPSLQQLKRARSDTV
RHFMNAKGGPAVAYTEQRVASIASAIPLTTDKTVIEANALMATALASQIKLVGDLIRTYDERIESLFDTLPDAELFKSLPGMGPCMGPRMLAALGDNRNR
FNSAEEIQNYAGIAPVTERSGQKSWVHWRWQCAKFVRQTFVEWTAKTVNASYWAKLYYQGQREKGKSHQSAIRALAFKWIRIIYRCWKTKTRYDEAKYLL
ALEERKSSLLMP
Blast result :
Comments
ISEch13 is 97% aa similar to ISCfr13.
In the genome, this IS is disrupted by insertion of ISEch14. This IS was in silico reconstructed after deletion of one copy of ISEch14 (IS3 family) and one of the DR generated by its insertion.
In the genome, this IS is disrupted by insertion of ISEch14. This IS was in silico reconstructed after deletion of one copy of ISEch14 (IS3 family) and one of the DR generated by its insertion.
References
1] ISfinder submission (2018)
2] Glasner,J.D., Yang,C.H., Reverchon,S., Hugouvieux-Cotte-Pattat,N., Condemine,G., Bohin,J.P., Van Gijsegem,F., Yang,S., Franza,T., Expert,D., Plunkett,G. III, San Francisco,M.J., Charkowski,A.O., Py,B., Bell,K., Rauscher,L., Rodriguez-Palenzuela,P., Toussaint,A., Holeva,M.C., He,S.Y., Douet,V., Boccara,M., Blanco,C., Toth,I., Anderson,B.D., Biehl,B.S., Mau,B., Flynn,S.M., Barras,F., Lindeberg,M., Birch,P.R., Tsuyumu,S., Shi,X., Hibbing,M., Yap,M.N., Carpentier,M., Dassa,E., Umehara,M., Kim,J.F., Rusch,M., Soni,P., Mayhew,G.F., Fouts,D.E., Gill,S.R., Blattner,F.R., Keen,N.T. and Perna,N.T. (2011) J. Bacteriol. 193 (8), 2076-2077.
2] Glasner,J.D., Yang,C.H., Reverchon,S., Hugouvieux-Cotte-Pattat,N., Condemine,G., Bohin,J.P., Van Gijsegem,F., Yang,S., Franza,T., Expert,D., Plunkett,G. III, San Francisco,M.J., Charkowski,A.O., Py,B., Bell,K., Rauscher,L., Rodriguez-Palenzuela,P., Toussaint,A., Holeva,M.C., He,S.Y., Douet,V., Boccara,M., Blanco,C., Toth,I., Anderson,B.D., Biehl,B.S., Mau,B., Flynn,S.M., Barras,F., Lindeberg,M., Birch,P.R., Tsuyumu,S., Shi,X., Hibbing,M., Yap,M.N., Carpentier,M., Dassa,E., Umehara,M., Kim,J.F., Rusch,M., Soni,P., Mayhew,G.F., Fouts,D.E., Gill,S.R., Blattner,F.R., Keen,N.T. and Perna,N.T. (2011) J. Bacteriol. 193 (8), 2076-2077.