ISfinder Mirror Site

ISEch12

Family IS3
Group IS407

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP002038	ND	Dickeya dadantii	Dickeya dadantii 3937

DNA section

IS Length : 1211 bp

Ends

IR Length : 24/34

IRL : TGATCTCCTCCCTGAAAACAGTGCCAGTCTAAACTGAAGTATCCGGTCTT
IRR : TGACCCTGACCCAAAATCAGTGCAGTGCTAAATCAGAGATCTTCGCCTTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AAGTTGTCGTG		ATGTCGGAAGA	0

DNA sequence

TGATCTCCTCCCTGAAAACAGTGCCAGTCTAAACTGAAGTATCCGGTCTTTCTTCCATCTCGCCGGGAGGCACATCGCCATGAAAAAGACCCGTTATACC
GAAGAACAGATTGCGTTTGCGCTGAAACAGGCCGAAACCGGCACCCGCGTCGGGGAAGTCTGCAGAAAGATGGGCATTTCTGAGGCCACCTTTTACAACT
GGAAAAAGAAATTTGCCGGTCTGGGCGTGACGGAACTGCGACGGCTGAGGCAACTGGAGGATGAAAATCAGCGCCTGAAGAAACTGGTGGCTGACCTGAG
TCTGGACAAAGAGATGCTTCAGGAGGTGCTGAAGCAAAAGTTCTGAGGCCGGCTCAGAAGCGCCAGGCGGTACATTTCCTGCTGGAGGCTTACCGTATCA
GCGTCCGGCGGGGTTGTGGGCTGCTGATGCAGAGCAGAACGGTTTACCACTGGCTGAGTCGGCGTGACGATCGGGCAATAACCCTGCGCATCAGGGAGAT
AGCAGAAACCCGGATACGCTACGGTTGTCCACGCATTCATATCCAGTTACGCCGGGAAGGCTGGCCTGTTAACCACAAGAAAACCCACCGGATTTATTGT
CTGGAAGGACTGAACCTGCGCAGAAAGCGGCCCCGCAGGCATGTCAGTGCAGCACGCCGTCAGCAGCGCCCGACGCTGACGCATGTCGATCAGTGCTGGA
GTATGGATTTTGTGTCGGATAATCTGTTTAACGGGCGGCGTTTCCGGGCACTGACTGTAGTGGATAATTTTAGTCGGGAATGTCTGGCGATCCATGCCGG
AAAATCACTGAAAGGTGACGATGTGGTCAGCGTAATGGAAGCGCTGCGGGTGCTGGATAAGCGCCTGCCGGTACGTATTCAGACGGATAACGGCAGCGAG
TTTATCTCGAAAAGTCTGGATAAGTGGGCGTATGAACACGGTGTCACGATGGACTTCTCCCGCCCCGGAAAACCGACAGATAATCCGTTTATTGAATCAT
TTAACGGTAGCCTGCGCGACGAATGCCTGAACATTCACTGGTTTTTATCACTGGAAGATGCGCAGGAGAAACTCGACAACTGGCGCAGGGAATATAATCA
TGAGAGAACGCATTCATCATTAAATGACATGACTCCGGCTGAATTTATCCGAAGTCTCCAGAAAGGCGAAGATCTCTGATTTAGCACTGCACTGATTTTG
GGTCAGGGTCA

Recoding section

Recoding by frameshift
Frame -1
Type translational
Experimentally demonstrated No

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
267 bp	88 aa	80	346	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKKTRYTEEQIAFALKQAETGTRVGEVCRKMGISEATFYNWKKKFAGLGVTELRRLRQLEDENQRLKKLVADLSLDKEMLQEVLKQKF

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
885 bp	294 aa	295	1179	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

PESGQRDASGGAEAKVLRPAQKRQAVHFLLEAYRISVRRGCGLLMQSRTVYHWLSRRDDRAITLRIREIAETRIRYGCPRIHIQLRREGWPVNHKKTHRI
YCLEGLNLRRKRPRRHVSAARRQQRPTLTHVDQCWSMDFVSDNLFNGRRFRALTVVDNFSRECLAIHAGKSLKGDDVVSVMEALRVLDKRLPVRIQTDNG
SEFISKSLDKWAYEHGVTMDFSRPGKPTDNPFIESFNGSLRDECLNIHWFLSLEDAQEKLDNWRREYNHERTHSSLNDMTPAEFIRSLQKGEDL

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1100 bp	366 aa	80	1179	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKKTRYTEEQIAFALKQAETGTRVGEVCRKMGISEATFYNWKKKFAGLGVTELRRLRQLEDENQRLKKLVADLSLDKEMLQEVLKQKVLRPAQKRQAVHF
LLEAYRISVRRGCGLLMQSRTVYHWLSRRDDRAITLRIREIAETRIRYGCPRIHIQLRREGWPVNHKKTHRIYCLEGLNLRRKRPRRHVSAARRQQRPTL
THVDQCWSMDFVSDNLFNGRRFRALTVVDNFSRECLAIHAGKSLKGDDVVSVMEALRVLDKRLPVRIQTDNGSEFISKSLDKWAYEHGVTMDFSRPGKPT
DNPFIESFNGSLRDECLNIHWFLSLEDAQEKLDNWRREYNHERTHSSLNDMTPAEFIRSLQKGEDL

Blast result :

Comments

ISEch12 is 89% (transposase) aa similar to ISYps8.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] ISfinder submission (2018)
2] Glasner,J.D., Yang,C.H., Reverchon,S., Hugouvieux-Cotte-Pattat,N., Condemine,G., Bohin,J.P., Van Gijsegem,F., Yang,S., Franza,T., Expert,D., Plunkett,G. III, San Francisco,M.J., Charkowski,A.O., Py,B., Bell,K., Rauscher,L., Rodriguez-Palenzuela,P., Toussaint,A., Holeva,M.C., He,S.Y., Douet,V., Boccara,M., Blanco,C., Toth,I., Anderson,B.D., Biehl,B.S., Mau,B., Flynn,S.M., Barras,F., Lindeberg,M., Birch,P.R., Tsuyumu,S., Shi,X., Hibbing,M., Yap,M.N., Carpentier,M., Dassa,E., Umehara,M., Kim,J.F., Rusch,M., Soni,P., Mayhew,G.F., Fouts,D.E., Gill,S.R., Blattner,F.R., Keen,N.T. and Perna,N.T. (2011) J. Bacteriol. 193 (8), 2076-2077.