ISAbe18

  • Family IS4
  • Group IS4Sa
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acinetobacter bereziniae
Acinetobacter bereziniae HPC229
DNA section
IS Length : 1269 bp

Ends


IR Length : 16/19

IRL : CACTGCTGTCCCATAGTTTGCTTTAAATAAAAAAACCTGAGTCCTAACAG
IRR : CATTGCTGTCCCACAATTTTTCACAAGAGGAAGCTCATTTGAGCTTCCTC

Insertion site


Left flankDirect repeatRight flankDR Length
aatgggacGtataggctcccattt9

DNA sequence

CACTGCTGTCCCATAGTTTGCTTTAAATAAAAAAACCTGAGTCCTAACAGTTAAAATATGAGTGTAAACAAACACTTTAACGACCAGGATTCAGGTTTTG
TCACATCAGAATACCGTATTTTATGAGCTAATTAAACCTGTTGTGCGACAGGATTTTGAACAACTTGCTAAAGTACACCATGTTGGACAGAAATTTAGAG
CGACTTCCCGTTGGGATCAGTTTATTGCCATATTGATGTCTCAATTCTCTTGTAGGCAAAGTCTGAGAGATATTCAATCCAATTTGGAGTGCCAACAGGA
AAAGCTAAGTCATCTCGGAGCAAAATCTATTCCCCGAAGCACGCTGGCACGAATCAATGAGCAGCAGCCTGCTGCCTTGTATCAACAGCTATTTTACAAG
TTGCTTAAATACTATGAACACTCAAAAGTAGCTCATAAATTTCGCTTTAAAAATCCCTTGTATTCCTTGGATGCCAGTCATATTGACCTGTCGCTTTCCT
TATGTGAATGGGCCAAAGTTCATGACTCAAAAGCCAGCATGAAACTCAGCATAGGATTGAATCACAGCAATGATATTCCTGAGTTTGTTGCAGTTGAAAA
TGGCAAAGAAAATGACATGGTACAAGGTCGCAAATTCCAGTTTCCTGCTGGCAGCATTGTAGTTTTTGATAAAGGCTATGTCGATTACCAATGGTATGCA
AATCTGACTGCTCAAAACATTGGATTTGTCACACGTTTTAGGCCTAAATCTGTGTATCAGGTGATCCAGCAACATCCAGTGCTTGAATCCAAAGGTATTC
TAAAAGATGAAACCATTCAGCTGAATAGCGCACATGCCCTAAAAAGAAAAGCCCCAGTGTTAAGAAGAATTGAATATAGAGATCAGCAAAGTGGCAAGCA
CTTTAGCTTTCTCAGCAATAACTTTCATTTAGCCGCCTCCACCATTGCGGCGATTTATAAAGATCGTTGGAAAGTTGAGCTGTTCTTTAAGGCGATTAAG
CAGAATCTCAAATTAAAAGCGTTTCTAGGCCGCAGCAGGAACGCAATTCAGACACAAATCTGGATTGCGATGATCGCCTATTTATTGGTGAGTTTCGCTC
AACATTTAGGGAAAACAGGTTGGACAGTTCAACGTTTACTCAGAATAATTCAAGTGAATTTGTTTGAAAGAAGAACTTTAAAAGCTTTATTTTCACCCGA
TAAAATACCCATAAAACAAGAGGAAGCTCAAATGAGCTTCCTCTTGTGAAAAATTGTGGGACAGCAATG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1152 bp383 aa981249+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSHQNTVFYELIKPVVRQDFEQLAKVHHVGQKFRATSRWDQFIAILMSQFSCRQSLRDIQSNLECQQEKLSHLGAKSIPRSTLARINEQQPAALYQQLFY
KLLKYYEHSKVAHKFRFKNPLYSLDASHIDLSLSLCEWAKVHDSKASMKLSIGLNHSNDIPEFVAVENGKENDMVQGRKFQFPAGSIVVFDKGYVDYQWY
ANLTAQNIGFVTRFRPKSVYQVIQQHPVLESKGILKDETIQLNSAHALKRKAPVLRRIEYRDQQSGKHFSFLSNNFHLAASTIAAIYKDRWKVELFFKAI
KQNLKLKAFLGRSRNAIQTQIWIAMIAYLLVSFAQHLGKTGWTVQRLLRIIQVNLFERRTLKALFSPDKIPIKQEEAQMSFLL

 

Blast result :
Comments
ISAbe18 is 78% aa similar to ISCja2.
References
1] Brovedan M.A. (2018) Direct submission.
2] Brovedan,M., Marchiaro,P.M., Moran-Barrio,J., Revale,S., Cameranesi,M., Brambilla,L., Viale,A.M. and Limansky,A.S. (2016) Genome Announc 4 (2).