ISAbe17
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| LKDJ01000094.1 | ND | Acinetobacter bereziniae | Acinetobacter bereziniae HPC229 Acinetobacter bereziniae AberCHI-40-1 |
DNA section
IS Length : 1313 bp
Ends
IR Length : 20/25
IRL : TGTCCCGTCCTGAAATAAGTTTACACATAGGAGACTTATTTTATGGAACA
IRR : TGTCTCGTCCTAAAATAGATTGACAGTATTTCATTTAGGACCGAGTTAAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTAGGGCTAG | GCT | TTTTACCTTT | 3 |
DNA sequence
TGTCCCGTCCTGAAATAAGTTTACACATAGGAGACTTATTTTATGGAACATGAACAAAGCCAAAGAGTTAAACGTACTCAGAGAGATTATTCTTTTGCCT
TTAAAATGATGGTTATTCAGGAAGTTGAAAAAGGGCAACTTACTTACAAGCAAGTTCAGGCAAAATATGGTATTCAAGGAAGATCAACAGTTCTCGTCTG
GCTTCGTAAGTTTGGACAACAAGATTGGATTTCAAAGACCATGCCAAAATCATCTAAACGTCAGCTGACACCACAGCAAAGAATCCGCCAATTAGAAAAA
CAATTGGCTGAAGAGAAATTGAAAACTGAATTTGTAGAAGATGTCATCTACCATCTCGACAAAAAATGTGGGACTGACTTCCAAAAAAAGTATATCGAGC
ACGTTTCGAAGATTGGAAAAGCAAAAGACGACTAAGTGTTACTCGTTTATGCCAATGGCTTAAGATCTCAAGACAAGCCTACTATCAATCTGAAAAACGA
GCTCAAGTTAGGGTACGACAAACACAACAAGTTCTTGATTTAGTGATGAGTTATCGTTATCTCATGCCAAGCATTGGTACACGTAAATTATATTGGTTAA
TTACCCCTCAATTGTTAGCTCAAGGATTAAAGATTGGAAGAGATAAGCTATTTAATATATTGAAAGAAAATAACTTATTAATTCGGCCTAAACGTCGTTA
TACCAAAACAACTGATAGCAAACATTGGATGAAGAAACATCCCAATTTATTAAAGAACTACAAACCGACCCAAGCGAATGAGGTTCTGGTGAGTGATATA
ACTTACGTTGAAAGTGTGGAAGGCGTACATTACCTATCCTTGGTAACAGATGCTTATACACGGCAAATTAAAGGATATAAATTATCGAAGGATATGAAGG
CAGAGAATGTTGTACAAGCCTTTGAGATGGCGATGAGTAAGCAAAGAGATCGAGCAAAAGTTATGATTCACCATTCAGATAGAGGTTCTCAATATTGTTC
AGAACTTTACCAATCTGCATTAAGTAAACATGGGGTTCTCCCCTCAATGACAGATGGCTATGATTGTTACCAGAACGCCTTAGCTGAAAGAATAAACGGG
ATATTGAAACAAGAGTTTCTAACGATACGTTGTAATAGTATGAAAGAATTAGATCAACTGATTGCAGAATCAATAGAAATATATAATTGTTATAGACCAC
ATTTGAGCCTAGGTATGATGACACCTAATCAAATGTTTGAAAAAGCAAGAACCGAGCTAATTGCTTAACTCGGTCCTAAATGAAATACTGTCAATCTATT
TTAGGACGAGACA
TTAAAATGATGGTTATTCAGGAAGTTGAAAAAGGGCAACTTACTTACAAGCAAGTTCAGGCAAAATATGGTATTCAAGGAAGATCAACAGTTCTCGTCTG
GCTTCGTAAGTTTGGACAACAAGATTGGATTTCAAAGACCATGCCAAAATCATCTAAACGTCAGCTGACACCACAGCAAAGAATCCGCCAATTAGAAAAA
CAATTGGCTGAAGAGAAATTGAAAACTGAATTTGTAGAAGATGTCATCTACCATCTCGACAAAAAATGTGGGACTGACTTCCAAAAAAAGTATATCGAGC
ACGTTTCGAAGATTGGAAAAGCAAAAGACGACTAAGTGTTACTCGTTTATGCCAATGGCTTAAGATCTCAAGACAAGCCTACTATCAATCTGAAAAACGA
GCTCAAGTTAGGGTACGACAAACACAACAAGTTCTTGATTTAGTGATGAGTTATCGTTATCTCATGCCAAGCATTGGTACACGTAAATTATATTGGTTAA
TTACCCCTCAATTGTTAGCTCAAGGATTAAAGATTGGAAGAGATAAGCTATTTAATATATTGAAAGAAAATAACTTATTAATTCGGCCTAAACGTCGTTA
TACCAAAACAACTGATAGCAAACATTGGATGAAGAAACATCCCAATTTATTAAAGAACTACAAACCGACCCAAGCGAATGAGGTTCTGGTGAGTGATATA
ACTTACGTTGAAAGTGTGGAAGGCGTACATTACCTATCCTTGGTAACAGATGCTTATACACGGCAAATTAAAGGATATAAATTATCGAAGGATATGAAGG
CAGAGAATGTTGTACAAGCCTTTGAGATGGCGATGAGTAAGCAAAGAGATCGAGCAAAAGTTATGATTCACCATTCAGATAGAGGTTCTCAATATTGTTC
AGAACTTTACCAATCTGCATTAAGTAAACATGGGGTTCTCCCCTCAATGACAGATGGCTATGATTGTTACCAGAACGCCTTAGCTGAAAGAATAAACGGG
ATATTGAAACAAGAGTTTCTAACGATACGTTGTAATAGTATGAAAGAATTAGATCAACTGATTGCAGAATCAATAGAAATATATAATTGTTATAGACCAC
ATTTGAGCCTAGGTATGATGACACCTAATCAAATGTTTGAAAAAGCAAGAACCGAGCTAATTGCTTAACTCGGTCCTAAATGAAATACTGTCAATCTATT
TTAGGACGAGACA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 393 bp | 130 aa | 43 | 435 | + | No |
Description : First part of the transposase
ORF sequence :
MEHEQSQRVKRTQRDYSFAFKMMVIQEVEKGQLTYKQVQAKYGIQGRSTVLVWLRKFGQQDWISKTMPKSSKRQLTPQQRIRQLEKQLAEEKLKTEFVED
VIYHLDKECGTDFQKKYIEHVSKIGKAKDD
VIYHLDKECGTDFQKKYIEHVSKIGKAKDD
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 891 bp | 296 aa | 378 | 1268 | + | No |
Description : Second part of the transposase
ORF sequence :
LPKKVYRARFEDWKSKRRLSVTRLCQWLKISRQAYYQSEKRAQVRVRQTQQVLDLVMSYRYLMPSIGTRKLYWLITPQLLAQGLKIGRDKLFNILKENNL
LIRPKRRYTKTTDSKHWMKKHPNLLKNYKPTQANEVLVSDITYVESVEGVHYLSLVTDAYTRQIKGYKLSKDMKAENVVQAFEMAMSKQRDRAKVMIHHS
DRGSQYCSELYQSALSKHGVLPSMTDGYDCYQNALAERINGILKQEFLTIRCNSMKELDQLIAESIEIYNCYRPHLSLGMMTPNQMFEKARTELIA
LIRPKRRYTKTTDSKHWMKKHPNLLKNYKPTQANEVLVSDITYVESVEGVHYLSLVTDAYTRQIKGYKLSKDMKAENVVQAFEMAMSKQRDRAKVMIHHS
DRGSQYCSELYQSALSKHGVLPSMTDGYDCYQNALAERINGILKQEFLTIRCNSMKELDQLIAESIEIYNCYRPHLSLGMMTPNQMFEKARTELIA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1226 bp | 408 aa | 43 | 1268 | + | Yes |
Chemistry : DDE
ORF sequence :
MEHEQSQRVKRTQRDYSFAFKMMVIQEVEKGQLTYKQVQAKYGIQGRSTVLVWLRKFGQQDWISKTMPKSSKRQLTPQQRIRQLEKQLAEEKLKTEFVED
VIYHLDKECGTDFQKKYIEHVSKIGKAKDDLSVTRLCQWLKISRQAYYQSEKRAQVRVRQTQQVLDLVMSYRYLMPSIGTRKLYWLITPQLLAQGLKIGR
DKLFNILKENNLLIRPKRRYTKTTDSKHWMKKHPNLLKNYKPTQANEVLVSDITYVESVEGVHYLSLVTDAYTRQIKGYKLSKDMKAENVVQAFEMAMSK
QRDRAKVMIHHSDRGSQYCSELYQSALSKHGVLPSMTDGYDCYQNALAERINGILKQEFLTIRCNSMKELDQLIAESIEIYNCYRPHLSLGMMTPNQMFE
KARTELIA
VIYHLDKECGTDFQKKYIEHVSKIGKAKDDLSVTRLCQWLKISRQAYYQSEKRAQVRVRQTQQVLDLVMSYRYLMPSIGTRKLYWLITPQLLAQGLKIGR
DKLFNILKENNLLIRPKRRYTKTTDSKHWMKKHPNLLKNYKPTQANEVLVSDITYVESVEGVHYLSLVTDAYTRQIKGYKLSKDMKAENVVQAFEMAMSK
QRDRAKVMIHHSDRGSQYCSELYQSALSKHGVLPSMTDGYDCYQNALAERINGILKQEFLTIRCNSMKELDQLIAESIEIYNCYRPHLSLGMMTPNQMFE
KARTELIA
Blast result :
Comments
ISAbe17 is 71% (transposase) aa similar to ISSpe1.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Brovedan M.A. (2018) Direct submission.
2] Brovedan,M., Marchiaro,P.M., Moran-Barrio,J., Revale,S., Cameranesi,M., Brambilla,L., Viale,A.M. and Limansky,A.S. (2016) Genome Announc 4 (2).
3] Jones S,Lim. (2014) Direct GenBank submission.
2] Brovedan,M., Marchiaro,P.M., Moran-Barrio,J., Revale,S., Cameranesi,M., Brambilla,L., Viale,A.M. and Limansky,A.S. (2016) Genome Announc 4 (2).
3] Jones S,Lim. (2014) Direct GenBank submission.