ISAbe16
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| LKDJ01000092 | ND | Acinetobacter bereziniae | Acinetobacter bereziniae HPC229 |
DNA section
IS Length : 1426 bp
Ends
IR Length : 20/25
IRL : TGAACTGAACCCTATAAGTTGGACACAACTTATAGGGTATTTTTATGAAA
IRR : TGGACTGAACCCCAAATCTTGGACAGTTTAGTTAACGCTGGATTGAGTTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCAAATAAAAACCACTC | ATGT | TGAGTGGTTTTTATT | 4 |
DNA sequence
TGAACTGAACCCTATAAGTTGGACACAACTTATAGGGTATTTTTATGAAATACGATTTAAAGTTTAAACAAGCAGTCATTGCTTATTATGCTCAAGGCCA
TTCTAGTTTATCAACAGCCAAGCATTTTTCGATTAGTGATAAGGATGTCTCTAAATGGTTGGCTCAATTCAACTTAGGTGGAATTGATGCAATAAAACCA
AAACAGAATAAGACAATTTATACAGCTGAATTTAAATATAAAGTATTAACAACTATGCAGGATGAGCGATTAAGTTTATCTGATACAGCTCTTCGTTTTG
GTATTTCTTCCCCGAGTTTGATCAGTGTATGGCGTAAAATCTATCAAACCGATGGTATGCTTGGACTAGAACCAAAGTCTAAAGGAAGAGTACCCATGTC
TAAAAATAAACATAAATATATTGTTGATAAACCTGATCATGAAAAAACAACTGCTGAGCTTCAGCGTGAACTACTCTATTTACGCGCTGAGAATGCTTAC
TTAAAAAAGCTCGACGCCTTGCTGAATCCACCCAAAAAGGACAACAAGCAAGGTTGATTGATCAGATGAGTACTGAGTTTGCTTTGCCATTGCTTTTAGA
TATTGCTGAGTTATCGAGGAGTAGTTTCTACTATCATAAAGCCAAGCTAAATGCCGTAGATAAATATGCAGAGCTCAAGCAACGAATTTGCCAAATTTAT
GATCAGCATAAAGGTAGATATGGTTATAGACGAATCACTTTAGCCTTGAAGCAACAAGGTCTTCATCATAACCATAAATTGATTGCTAGGTTGATGAGAG
GCTTTGGTTTAATTTCATTGATTCGGCGTAAAAAGTACAATGCCTACAAAGGTACATCCAATAAAATAGTCAAAAATAAACTCAAACGTAACTTTGAAGC
GAAGCGACCCAATAAGTGTTGGCTGACAGATATAACCGAATTTAAAGTTGCTGATACGAAGTTATATTTATCTCCGATTTTAGACTGCTATAACAATGAG
ATTGTGAGCTATACACTTTCAAGACGCCCAGTATTTCAACTTGTTAAAGATATGCTTGATCAAGCCATAAGTCATTTACCAAACTCACGAAAACACAAGC
TTATGCTTCATTCAGATCAAGGCTGGCATTATCAAATGAGCCAATTTAGGAGTACGCTTAAAGCGAATAAGATTAAGCAAAGTATGAGTAGAAAAGGGAA
TTGTTGGGACAATGCTTTGATGGAGAGTTTCTTTGGTACATTAAAATCAGAGACGATTTATCTAGAGAAACCAGAAAGCATTGAAGTCTTAGAGCAACAA
ATTCATGACTACATCCATTACTATAATCATGAGAGAATACAAATTAAACTAAAAGGATTGAGCCCTGTTCAATTCAGAACTCAATCCAGCGTTAACTAAA
CTGTCCAAGATTTGGGGTTCAGTCCA
TTCTAGTTTATCAACAGCCAAGCATTTTTCGATTAGTGATAAGGATGTCTCTAAATGGTTGGCTCAATTCAACTTAGGTGGAATTGATGCAATAAAACCA
AAACAGAATAAGACAATTTATACAGCTGAATTTAAATATAAAGTATTAACAACTATGCAGGATGAGCGATTAAGTTTATCTGATACAGCTCTTCGTTTTG
GTATTTCTTCCCCGAGTTTGATCAGTGTATGGCGTAAAATCTATCAAACCGATGGTATGCTTGGACTAGAACCAAAGTCTAAAGGAAGAGTACCCATGTC
TAAAAATAAACATAAATATATTGTTGATAAACCTGATCATGAAAAAACAACTGCTGAGCTTCAGCGTGAACTACTCTATTTACGCGCTGAGAATGCTTAC
TTAAAAAAGCTCGACGCCTTGCTGAATCCACCCAAAAAGGACAACAAGCAAGGTTGATTGATCAGATGAGTACTGAGTTTGCTTTGCCATTGCTTTTAGA
TATTGCTGAGTTATCGAGGAGTAGTTTCTACTATCATAAAGCCAAGCTAAATGCCGTAGATAAATATGCAGAGCTCAAGCAACGAATTTGCCAAATTTAT
GATCAGCATAAAGGTAGATATGGTTATAGACGAATCACTTTAGCCTTGAAGCAACAAGGTCTTCATCATAACCATAAATTGATTGCTAGGTTGATGAGAG
GCTTTGGTTTAATTTCATTGATTCGGCGTAAAAAGTACAATGCCTACAAAGGTACATCCAATAAAATAGTCAAAAATAAACTCAAACGTAACTTTGAAGC
GAAGCGACCCAATAAGTGTTGGCTGACAGATATAACCGAATTTAAAGTTGCTGATACGAAGTTATATTTATCTCCGATTTTAGACTGCTATAACAATGAG
ATTGTGAGCTATACACTTTCAAGACGCCCAGTATTTCAACTTGTTAAAGATATGCTTGATCAAGCCATAAGTCATTTACCAAACTCACGAAAACACAAGC
TTATGCTTCATTCAGATCAAGGCTGGCATTATCAAATGAGCCAATTTAGGAGTACGCTTAAAGCGAATAAGATTAAGCAAAGTATGAGTAGAAAAGGGAA
TTGTTGGGACAATGCTTTGATGGAGAGTTTCTTTGGTACATTAAAATCAGAGACGATTTATCTAGAGAAACCAGAAAGCATTGAAGTCTTAGAGCAACAA
ATTCATGACTACATCCATTACTATAATCATGAGAGAATACAAATTAAACTAAAAGGATTGAGCCCTGTTCAATTCAGAACTCAATCCAGCGTTAACTAAA
CTGTCCAAGATTTGGGGTTCAGTCCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 513 bp | 170 aa | 45 | 557 | + | No |
Description : First part of the transposase
ORF sequence :
MKYDLKFKQAVIAYYAQGHSSLSTAKHFSISDKDVSKWLHQFNLGGIDAIKPKQNKTIYTAEFKYKVLTTMQDERLSLSDTALRFGISSPSLISVWRKIY
QTDGMLGLEPKSKGRVPMSKNKHKYIVDKPDHEKTTAELQRELLYLRAENAYLKKLDALLNPPKKDNKQG
QTDGMLGLEPKSKGRVPMSKNKHKYIVDKPDHEKTTAELQRELLYLRAENAYLKKLDALLNPPKKDNKQG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 909 bp | 302 aa | 491 | 1399 | + | No |
Description : Second part of the transposase
ORF sequence :
ECLLKKARRLAESTQKGQQARLIDQMSTEFALPLLLDIAELSRSSFYYHKAKLNAVDKYAELKQRICQIYDQHKGRYGYRRITLALKQQGLHHNHKLIAR
LMRGFGLISLIRRKKYNAYKGTSNKIVKNKLKRNFEAKRPNKRWLTDITEFKVADTKLYLSPILDCYNNEIVSYTLSRRPVFQLVKDMLDQAISHLPNSR
KHKLMLHSDQGWHYQMSQFRSTLKANKIKQSMSRKGNCWDNALMESFFGTLKSETIYLEKPESIEVLEQQIHDYIHYYNHERIQIKLKGLSPVQFRTQSN
VN
LMRGFGLISLIRRKKYNAYKGTSNKIVKNKLKRNFEAKRPNKRWLTDITEFKVADTKLYLSPILDCYNNEIVSYTLSRRPVFQLVKDMLDQAISHLPNSR
KHKLMLHSDQGWHYQMSQFRSTLKANKIKQSMSRKGNCWDNALMESFFGTLKSETIYLEKPESIEVLEQQIHDYIHYYNHERIQIKLKGLSPVQFRTQSN
VN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1355 bp | 450 aa | 45 | 1399 | + | Yes |
Chemistry : DDE
ORF sequence :
MKYDLKFKQAVIAYYAQGHSSLSTAKHFSISDKDVSKWLHQFNLGGIDAIKPKQNKTIYTAEFKYKVLTTMQDERLSLSDTALRFGISSPSLISVWRKIY
QTDGMLGLEPKSKGRVPMSKNKHKYIVDKPDHEKTTAELQRELLYLRAENAYLKKARRLAESTQKGQQARLIDQMSTEFALPLLLDIAELSRSSFYYHKA
KLNAVDKYAELKQRICQIYDQHKGRYGYRRITLALKQQGLHHNHKLIARLMRGFGLISLIRRKKYNAYKGTSNKIVKNKLKRNFEAKRPNKRWLTDITEF
KVADTKLYLSPILDCYNNEIVSYTLSRRPVFQLVKDMLDQAISHLPNSRKHKLMLHSDQGWHYQMSQFRSTLKANKIKQSMSRKGNCWDNALMESFFGTL
KSETIYLEKPESIEVLEQQIHDYIHYYNHERIQIKLKGLSPVQFRTQSNVN
QTDGMLGLEPKSKGRVPMSKNKHKYIVDKPDHEKTTAELQRELLYLRAENAYLKKARRLAESTQKGQQARLIDQMSTEFALPLLLDIAELSRSSFYYHKA
KLNAVDKYAELKQRICQIYDQHKGRYGYRRITLALKQQGLHHNHKLIARLMRGFGLISLIRRKKYNAYKGTSNKIVKNKLKRNFEAKRPNKRWLTDITEF
KVADTKLYLSPILDCYNNEIVSYTLSRRPVFQLVKDMLDQAISHLPNSRKHKLMLHSDQGWHYQMSQFRSTLKANKIKQSMSRKGNCWDNALMESFFGTL
KSETIYLEKPESIEVLEQQIHDYIHYYNHERIQIKLKGLSPVQFRTQSNVN
Blast result :
Comments
ISAbe16 is 64% (transposase) aa similar to ISAba45.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Brovedan M.A. (2018) Direct submission.
2] Brovedan,M., Marchiaro,P.M., Moran-Barrio,J., Revale,S., Cameranesi,M., Brambilla,L., Viale,A.M. and Limansky,A.S. (2016) Genome Announc 4 (2).
3] Cerqueira,G., Feldgarden,M., Courvalin,P., Perichon,B., Grillot-Courvalin,C., Clermont,D., Rocha,E., Yoon,E.-J., Nemec,A., Walker,B., Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B., Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A.M., Chapman,S.B., Dewar,J., Goldberg,J., Griggs,A., Gujja,S., Hansen,M., Howarth,C., Imamovic,A., Larimer,J., McCowan,C., Murphy,C., Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T., Sisk,P., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.(2013) Direct GenBank submission.
2] Brovedan,M., Marchiaro,P.M., Moran-Barrio,J., Revale,S., Cameranesi,M., Brambilla,L., Viale,A.M. and Limansky,A.S. (2016) Genome Announc 4 (2).
3] Cerqueira,G., Feldgarden,M., Courvalin,P., Perichon,B., Grillot-Courvalin,C., Clermont,D., Rocha,E., Yoon,E.-J., Nemec,A., Walker,B., Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B., Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A.M., Chapman,S.B., Dewar,J., Goldberg,J., Griggs,A., Gujja,S., Hansen,M., Howarth,C., Imamovic,A., Larimer,J., McCowan,C., Murphy,C., Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T., Sisk,P., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.(2013) Direct GenBank submission.