ISAbe15
- Family IS5
- Group ISL2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| APPK01000036 | ND | Acinetobacter bereziniae | Acinetobacter bereziniae NIPH 3 Acinetobacter bereziniae HPC229 |
DNA section
IS Length : 869 bp
Ends
IR Length : 15/17
IRL : AGACTTCTTGCGAAAGTATAAAGTGTTTATAATAGCTTAATGAAATATGA
IRR : AGATCTCTTGCGAAAGTTATTTTTTAATCATCCAATTGATCAATCCTGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| atctcaac | ATT | aaaattct | 3 |
| CTATAATAGT | AATTTCTGAT | 0 |
DNA sequence
AGACTTCTTGCGAAAGTATAAAGTGTTTATAATAGCTTAATGAAATATGAAAATTTAACAAAATTCGATGATATTGAATTCAAGCGTCTAGTTGGAGTGA
CACGCCCCTTATTTTCTAAAATGATTCTTGTTTTAGAAGAGGCTGAACGTTTGAAGAAAAAATCTGGTCGCCCTCACAGCTTAGTACTAGAAGATCAATT
GTTATTAACATTGAAGTACTTACGCTCTTATAGTACTCAGCTTGAATTGGCTGCTGAGTTTGCAATAGCTGAAAGCAATGTCAATCGAACCATCCAAAAA
ATTGAGAATGGGCTTGTACAGTCTAAAGTATTTTCCTTACCAAAAAGAAATAAGGAAATTGCAAATCATGACTTTGTTATTGTAGACGTAACAGAATCAG
CGATAGAACGTCCTAAAAAAACAAATGCAGTTCTATAGTGGTAAAAAGAAAAAGCATACATTTAAAACTCAAATCATTTTTTGCCCTAAATGCAATCAGA
TACTGAGTATTCAGATAGATACAGGAAGAGTTCATGATTTAACTATTGCTCGACGCCATGCAAAAGAATTTTCTCAATTTGTTTGTATAATGGCAGATTT
AGCCTATAAAGGTTTTAAGGAAATTAAGGGTAAGTTGTTAATTCCAATAAAGAAACCGAAGAAAATGCAATTACCCAAAGAAGCTAAACGAATAAATAAA
GAAATAAGTCGACGTAGAATTTCAATAGAACATATTAATTCAAAATTAAAGACGTTTCGCATATTGTCTCAGCGCTATAGGAATAGACGTAAACGCTTTG
GTTTAAGAATGAATTTGATAGCAGGATTGATCAATTGGATGATTAAAAAATAACTTTCGCAAGAGATCT
CACGCCCCTTATTTTCTAAAATGATTCTTGTTTTAGAAGAGGCTGAACGTTTGAAGAAAAAATCTGGTCGCCCTCACAGCTTAGTACTAGAAGATCAATT
GTTATTAACATTGAAGTACTTACGCTCTTATAGTACTCAGCTTGAATTGGCTGCTGAGTTTGCAATAGCTGAAAGCAATGTCAATCGAACCATCCAAAAA
ATTGAGAATGGGCTTGTACAGTCTAAAGTATTTTCCTTACCAAAAAGAAATAAGGAAATTGCAAATCATGACTTTGTTATTGTAGACGTAACAGAATCAG
CGATAGAACGTCCTAAAAAAACAAATGCAGTTCTATAGTGGTAAAAAGAAAAAGCATACATTTAAAACTCAAATCATTTTTTGCCCTAAATGCAATCAGA
TACTGAGTATTCAGATAGATACAGGAAGAGTTCATGATTTAACTATTGCTCGACGCCATGCAAAAGAATTTTCTCAATTTGTTTGTATAATGGCAGATTT
AGCCTATAAAGGTTTTAAGGAAATTAAGGGTAAGTTGTTAATTCCAATAAAGAAACCGAAGAAAATGCAATTACCCAAAGAAGCTAAACGAATAAATAAA
GAAATAAGTCGACGTAGAATTTCAATAGAACATATTAATTCAAAATTAAAGACGTTTCGCATATTGTCTCAGCGCTATAGGAATAGACGTAAACGCTTTG
GTTTAAGAATGAATTTGATAGCAGGATTGATCAATTGGATGATTAAAAAATAACTTTCGCAAGAGATCT
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 399 bp | 132 aa | 40 | 438 | + | No |
Description : First part of the transposase
ORF sequence :
MKYENLTKFDDIEFKRLVGVTRPLFSKMILVLEEAERLKKKSGRPHSLVLEDQLLLTLKYLRSYSTQLELAAEFAIAESNVNRTIQKIENGLVQSKVFSL
PKRNKEIANHDFVIVDVTESAIERPKKTNAVL
PKRNKEIANHDFVIVDVTESAIERPKKTNAVL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 447 bp | 148 aa | 407 | 853 | + | No |
Description : Second part of the transposase
ORF sequence :
NVLKKQMQFYSGKKKKHTFKTQIIFCPKCNQILSIQIDTGRVHDLTIARRHAKEFSQFVCIMADLAYKGFKEIKGKLLIPIKKPKKMQLPKEAKRINKEI
SRRRISIEHINSKLKTFRILSQRYRNRRKRFGLRMNLIAGLINWMIKK
SRRRISIEHINSKLKTFRILSQRYRNRRKRFGLRMNLIAGLINWMIKK
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 814 bp | 270 aa | 40 | 853 | + | Yes |
Chemistry : DDE
ORF sequence :
MKYENLTKFDDIEFKRLVGVTRPLFSKMILVLEEAERLKKKSGRPHSLVLEDQLLLTLKYLRSYSTQLELAAEFAIAESNVNRTIQKIENGLVQSKVFSL
PKRNKEIANHDFVIVDVTESAIERPKKQMQFYSGKKKKHTFKTQIIFCPKCNQILSIQIDTGRVHDLTIARRHAKEFSQFVCIMADLAYKGFKEIKGKLL
IPIKKPKKMQLPKEAKRINKEISRRRISIEHINSKLKTFRILSQRYRNRRKRFGLRMNLIAGLINWMIKK
PKRNKEIANHDFVIVDVTESAIERPKKQMQFYSGKKKKHTFKTQIIFCPKCNQILSIQIDTGRVHDLTIARRHAKEFSQFVCIMADLAYKGFKEIKGKLL
IPIKKPKKMQLPKEAKRINKEISRRRISIEHINSKLKTFRILSQRYRNRRKRFGLRMNLIAGLINWMIKK
Blast result :
Comments
ISAbe15 is 76% (transposase) aa similar to ISAba27.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
This IS is disrupted by a copy of ISAbe6 (IS3 family), the sequence was reconstructed in silico by deletion of the ISAbe6 sequence and one of the direct repeat (4 bp) generated by this insertion.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
This IS is disrupted by a copy of ISAbe6 (IS3 family), the sequence was reconstructed in silico by deletion of the ISAbe6 sequence and one of the direct repeat (4 bp) generated by this insertion.
References
1] Brovedan M.A. (2018) Direct submission.
2] Brovedan,M., Marchiaro,P.M., Moran-Barrio,J., Revale,S., Cameranesi,M., Brambilla,L., Viale,A.M. and Limansky,A.S. (2016) Genome Announc 4 (2).
3] Cerqueira,G., Feldgarden,M., Courvalin,P., Perichon,B., Grillot-Courvalin,C., Clermont,D., Rocha,E., Yoon,E.-J., Nemec,A., Walker,B., Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B., Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A.M., Chapman,S.B., Dewar,J., Goldberg,J., Griggs,A., Gujja,S., Hansen,M., Howarth,C., Imamovic,A., Larimer,J., McCowan,C., Murphy,C., Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T., Sisk,P., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.(2013) Direct GenBank submission.
2] Brovedan,M., Marchiaro,P.M., Moran-Barrio,J., Revale,S., Cameranesi,M., Brambilla,L., Viale,A.M. and Limansky,A.S. (2016) Genome Announc 4 (2).
3] Cerqueira,G., Feldgarden,M., Courvalin,P., Perichon,B., Grillot-Courvalin,C., Clermont,D., Rocha,E., Yoon,E.-J., Nemec,A., Walker,B., Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B., Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A.M., Chapman,S.B., Dewar,J., Goldberg,J., Griggs,A., Gujja,S., Hansen,M., Howarth,C., Imamovic,A., Larimer,J., McCowan,C., Murphy,C., Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T., Sisk,P., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.(2013) Direct GenBank submission.