ISAbe12
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| BBLJ01000004 | ND | Acinetobacter bereziniae | Acinetobacter bereziniae HPC229 |
DNA section
IS Length : 1236 bp
Ends
IR Length : 18/24
IRL : TGATCCTAGCCAAAGTAATGGACACACTGTCCCTCTGAAGATAATGTAAC
IRR : TGATCCTAGACACTATCGTGGACAGTACTTCCCTCTAAATTCTTAAAATA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ttagtagt | aat | aattgttg | 3 |
| ATTAATACCC | GAGTTTCCCT | 0 |
DNA sequence
TGATCCTAGCCAAAGTAATGGACACACTGTCCCTCTGAAGATAATGTAACGTTAACTTTGGAGATGTACACATGGCTAAACGCTTTAGTCCAGAATTTAA
ACAGCAAGCAATTGATTATGCACTTGCGAACTCACATGAATCATTAGCATCAATTGCGGTAAAGCTTGGTGTTGGTTACTCGACACTTGATAAATGGATT
CGTACTGCTAATCCAGAAGGATCAAGCAAACGTCAGTTAACACCTGAGCAACAGCGAATATTAGATCTAGAAAAAGAAGTCAAACAACTCAAAGAGGCAA
ATGACATACTAAAAAAAGTGCATGTGTATTTCCTCACCGATCAAGGCAAGAGAAGTACACGGTAATCAAAGACTTTGCAATGGAAAAGGTTAAAGTCACT
GTACTGTGCAAACAATTAGGTGTCAGTACTTCGGGGTTCTATGCATGGAGAAAACGTCAGGTACGTATAGCAAAGCAGTATGATGATTTACAAGCGGTGT
ATTGGCAGCACCACGCTCGTTTAGGTGCACCATCCCTCGTTCATGATATGCATGACCTAGGTTACAGCATGAGTGTGCGCACAATAGGTCGAATGCTTAA
AAAACCAGGATTACACAGTAAAATTGCCCGTAAATATAGGCATACGACTGATTCAAATCATCGTTTGCCAACAGCACCAAATTTGTTAGATCAACAGTTC
ACTGTAACTGAGCCAAATAAGGTCTGGACGACTGATATTACGTATATTCGAACGAAGGAAGGCTGGTTATATTTATGCGTGATACTAGATCTATTTAGCC
GACGTATCGTCGGTTGGCAGACAAGTACACGAATAGATCGTCAATTGGTATGTGATGCGTTTAACTATGCACTTGCACGTCAGGGTTATCCAACAGGCTT
GATGATTCATTCGGATCAAGGCAGTCAATATTGCAGTCGCGATTTCAGAGCTGTATTACTGACAAATAAGGCAATACAAAGTATGTCACGTAGGGGAAAT
TGTTGGGATAACGCAGTAACAGAAAGTTTTTTCCATACATTGAAAGGGCATGTGGTACATGACAGTGTATTTTCTACAAGAAAAGAGGCAAATTCGGTCT
TGTTTGAATATATTGAAGTTTACTACAATCGAATCAGAAGGCATTCTGCAAATGGTTGGTTAAGCCCTGAAGCTTTTGAGCAGAAATATTTTAAGAATTT
AGAGGGAAGTACTGTCCACGATAGTGTCTAGGATCA
ACAGCAAGCAATTGATTATGCACTTGCGAACTCACATGAATCATTAGCATCAATTGCGGTAAAGCTTGGTGTTGGTTACTCGACACTTGATAAATGGATT
CGTACTGCTAATCCAGAAGGATCAAGCAAACGTCAGTTAACACCTGAGCAACAGCGAATATTAGATCTAGAAAAAGAAGTCAAACAACTCAAAGAGGCAA
ATGACATACTAAAAAAAGTGCATGTGTATTTCCTCACCGATCAAGGCAAGAGAAGTACACGGTAATCAAAGACTTTGCAATGGAAAAGGTTAAAGTCACT
GTACTGTGCAAACAATTAGGTGTCAGTACTTCGGGGTTCTATGCATGGAGAAAACGTCAGGTACGTATAGCAAAGCAGTATGATGATTTACAAGCGGTGT
ATTGGCAGCACCACGCTCGTTTAGGTGCACCATCCCTCGTTCATGATATGCATGACCTAGGTTACAGCATGAGTGTGCGCACAATAGGTCGAATGCTTAA
AAAACCAGGATTACACAGTAAAATTGCCCGTAAATATAGGCATACGACTGATTCAAATCATCGTTTGCCAACAGCACCAAATTTGTTAGATCAACAGTTC
ACTGTAACTGAGCCAAATAAGGTCTGGACGACTGATATTACGTATATTCGAACGAAGGAAGGCTGGTTATATTTATGCGTGATACTAGATCTATTTAGCC
GACGTATCGTCGGTTGGCAGACAAGTACACGAATAGATCGTCAATTGGTATGTGATGCGTTTAACTATGCACTTGCACGTCAGGGTTATCCAACAGGCTT
GATGATTCATTCGGATCAAGGCAGTCAATATTGCAGTCGCGATTTCAGAGCTGTATTACTGACAAATAAGGCAATACAAAGTATGTCACGTAGGGGAAAT
TGTTGGGATAACGCAGTAACAGAAAGTTTTTTCCATACATTGAAAGGGCATGTGGTACATGACAGTGTATTTTCTACAAGAAAAGAGGCAAATTCGGTCT
TGTTTGAATATATTGAAGTTTACTACAATCGAATCAGAAGGCATTCTGCAAATGGTTGGTTAAGCCCTGAAGCTTTTGAGCAGAAATATTTTAAGAATTT
AGAGGGAAGTACTGTCCACGATAGTGTCTAGGATCA
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 294 bp | 97 aa | 72 | 365 | + | No |
Description : First part of the transposase
ORF sequence :
MAKRFSPEFKQQAIDYALANSHESLASIAVKLGVGYSTLDKWIRTANPEGSSKRQLTPEQQRILDLEKEVKQLKEANDILKKVHVYFLTDQGKRSTR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 927 bp | 308 aa | 305 | 1231 | + | No |
Description : Second part of the transposase
ORF sequence :
HTKKSACVFPHRSRQEKYTVIKDFAMEKVKVTVLCKQLGVSTSGFYAWRKRQVRIAKQYDDLQAVYWQHHARLGAPSLVHDMHDLGYSMSVRTIGRMLKK
PGLHSKIARKYRHTTDSNHRLPTAPNLLDQQFTVTEPNKVWTTDITYIRTKEGWLYLCVILDLFSRRIVGWQTSTRIDRQLVCDAFNYALARQGYPTGLM
IHSDQGSQYCSRDFRAVLLTNKAIQSMSRRGNCWDNAVTESFFHTLKGHVVHDSVFSTRKEANSVLFEYIEVYYNRIRRHSANGWLSPEAFEQKYFKNLE
GSTVHDSV
PGLHSKIARKYRHTTDSNHRLPTAPNLLDQQFTVTEPNKVWTTDITYIRTKEGWLYLCVILDLFSRRIVGWQTSTRIDRQLVCDAFNYALARQGYPTGLM
IHSDQGSQYCSRDFRAVLLTNKAIQSMSRRGNCWDNAVTESFFHTLKGHVVHDSVFSTRKEANSVLFEYIEVYYNRIRRHSANGWLSPEAFEQKYFKNLE
GSTVHDSV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1160 bp | 386 aa | 72 | 1231 | + | No |
Chemistry : DDE
ORF sequence :
MAKRFSPEFKQQAIDYALANSHESLASIAVKLGVGYSTLDKWIRTANPEGSSKRQLTPEQQRILDLEKEVKQLKEANDILKKSACVFPHRSRQEKYTVIK
DFAMEKVKVTVLCKQLGVSTSGFYAWRKRQVRIAKQYDDLQAVYWQHHARLGAPSLVHDMHDLGYSMSVRTIGRMLKKPGLHSKIARKYRHTTDSNHRLP
TAPNLLDQQFTVTEPNKVWTTDITYIRTKEGWLYLCVILDLFSRRIVGWQTSTRIDRQLVCDAFNYALARQGYPTGLMIHSDQGSQYCSRDFRAVLLTNK
AIQSMSRRGNCWDNAVTESFFHTLKGHVVHDSVFSTRKEANSVLFEYIEVYYNRIRRHSANGWLSPEAFEQKYFKNLEGSTVHDSV
DFAMEKVKVTVLCKQLGVSTSGFYAWRKRQVRIAKQYDDLQAVYWQHHARLGAPSLVHDMHDLGYSMSVRTIGRMLKKPGLHSKIARKYRHTTDSNHRLP
TAPNLLDQQFTVTEPNKVWTTDITYIRTKEGWLYLCVILDLFSRRIVGWQTSTRIDRQLVCDAFNYALARQGYPTGLMIHSDQGSQYCSRDFRAVLLTNK
AIQSMSRRGNCWDNAVTESFFHTLKGHVVHDSVFSTRKEANSVLFEYIEVYYNRIRRHSANGWLSPEAFEQKYFKNLEGSTVHDSV
Blast result :
Comments
ISAbe12 is 92% aa similar to ISAcsp3.
The third is a putative ORFAB transposase reconstructed in silico by a possible frameshift.
The third is a putative ORFAB transposase reconstructed in silico by a possible frameshift.
References
1] Brovedan M.A. (2018) Direct submission.
2] Brovedan,M., Marchiaro,P.M., Moran-Barrio,J., Revale,S., Cameranesi,M., Brambilla,L., Viale,A.M. and Limansky,A.S. (2016) Genome Announc 4 (2).
3] Hosoyama,A., Hashimoto,M., Hosoyama,Y., Noguchi,M., Uohara,A., Shimodaira,J., Ohji,S., Ichikawa,N., Kimura,A., Yamazoe,A. and Fujita,N.(2014) Direct GenBank submission.
2] Brovedan,M., Marchiaro,P.M., Moran-Barrio,J., Revale,S., Cameranesi,M., Brambilla,L., Viale,A.M. and Limansky,A.S. (2016) Genome Announc 4 (2).
3] Hosoyama,A., Hashimoto,M., Hosoyama,Y., Noguchi,M., Uohara,A., Shimodaira,J., Ohji,S., Ichikawa,N., Kimura,A., Yamazoe,A. and Fujita,N.(2014) Direct GenBank submission.