ISAbe9

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
LKDJ01000013 ND Acinetobacter bereziniae
Acinetobacter bereziniae HPC229
DNA section
IS Length : 997 bp

Ends


IR Length : 27/38

IRL : TAGATAAATATATAAAGTTTGCTACACTATAAAAAACCTTACTGACTGCT
IRR : TAGCTAGTTAAATAAACTATGCTACATTTGATTAAACCCAATATCTAAAG

Insertion site


Left flankDirect repeatRight flankDR Length
TTTGAATGATTAGGAGTACTTT2

DNA sequence

TAGATAAATATATAAAGTTTGCTACACTATAAAAAACCTTACTGACTGCTGTTATGCCTAAGCCATACTCCAATGATCTTCGATACAAAGCTCTAGACTA
CTACAAAGAATGCCAGAATAAGTCTAAAACATGTACTGTTTTTAAGATTGCAAGAACGACTTTAGATCTCTGGATTCAGCTCGAACATCTCCAAGGAAAT
GCGAATAGACCACATCCCGAAAAAGCAGGTCGACCCTATAAAATTAAAGACCTAAAGGCTTTCAAAATCTTTGTTGAAACAACATCATTTTCACAGGCTA
AAGATCTGGTGCCATTATTTGAGCAAAGATTTGGTTATTCCGTCAGCTATGCCAATATCTTATTTGCTTTAAAAAAGCTGGGATGGAGTTATAAAAAAAG
AGTTTTCTCTACAGACAAGCCTGCAAAATAGGACGAGCAGTATTCCAATGGTTCCTGCCACAATGGAAACAGCAATATGGTGAAGAGTATATTTTATACA
TTGATGAATCAGGTATAAATACCAATGAGGTTGCTGAATATGGTTGGTCTGAGAAAGGAAAACGTTGTCGTGCATTAAGAACAGGTGGGCATAGAAGTAG
ACTAAATATAATCAGTGCTGTTCGATCAAGTGCAACGTTTAAATTCATTGCTCCCCTAATTTTTAAGGGCTCATGTGATCGCAATACTTTTACTGGCTGG
CTTGAATATTTACTCAAAGATCTAACAAGAGATAAAAATGGTCAGCCTCAAAAATATTTACTAATTTTAGATAATGCTTCTATTCACAAAGGTCGAAAAA
TTGAGGAATTGGCTCGCCATTACAATGCTAGGCTGATGTATTTGCCTGCTTATAGTCCAGACCTCAATCCAATTGAAAAAGCTTGGTCTATACTAAAAAG
AAAAGTCAGACATATTGTTAGCCAACAACAGAAAACTATTCTTGAGGCTTTAGATATTGGGTTTAATCAAATGTAGCATAGTTTATTTAACTAGCTA
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
378 bp125 aa54431+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MPKPYSNDLRYKALDYYKECQNKSKTCTVFKIARTTLDLWIQLEHLQGNANRPHPEKAGRPYKIKDLKAFKIFVETTSFSQAKDLVPLFEQRFGYSVSYA
NILFALKKLGWSYKKRVFSTDKPAK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
582 bp193 aa395976+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

KKSFLYRQACKIGRAVFQWFLPQWKQQYGEEYILYIDESGINTNEVAEYGWSEKGKRCRALRTGGHRSRLNIISAVRSSATFKFIAPLIFKGSCDRNTFT
GWLEYLLKDLTRDKNGQPQKYLLILDNASIHKGRKIEELARHYNARLMYLPAYSPDLNPIEKAWSILKRKVRHIVSQQQKTILEALDIGFNQM

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
923 bp307 aa54976+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPKPYSNDLRYKALDYYKECQNKSKTCTVFKIARTTLDLWIQLEHLQGNANRPHPEKAGRPYKIKDLKAFKIFVETTSFSQAKDLVPLFEQRFGYSVSYA
NILFALKKLGWSYKKKSFLYRQACKIGRAVFQWFLPQWKQQYGEEYILYIDESGINTNEVAEYGWSEKGKRCRALRTGGHRSRLNIISAVRSSATFKFIA
PLIFKGSCDRNTFTGWLEYLLKDLTRDKNGQPQKYLLILDNASIHKGRKIEELARHYNARLMYLPAYSPDLNPIEKAWSILKRKVRHIVSQQQKTILEAL
DIGFNQM

 

Blast result :
Comments
ISAbe9 is 59% aa similar to ISAtsp1.
The third is a putative ORFAB transposase reconstructed in silico by a possible frameshift.
References
1] Brovedan M.A. (2018) Direct submission.
2] Brovedan,M., Marchiaro,P.M., Moran-Barrio,J., Revale,S., Cameranesi,M., Brambilla,L., Viale,A.M. and Limansky,A.S. (2016) Genome Announc 4 (2).