ISfinder Mirror Site

ISAbe8

Family IS30
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
LKDJ01000054	ND	Acinetobacter bereziniae	Acinetobacter bereziniae HPC229

DNA section

IS Length : 1077 bp

Ends

IR Length : 20/27

IRL : GGCAGACTAAAACTTGAAGTGCGACAGCGCATCCCAATTTAATTTAGTTT
IRR : AGCGTATTCAAACATGAAGTGTGACAGATTTAAAAGCCTGATGATAATCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ATTTCATACA	agg	TTTATGCTTA	3

DNA sequence

GGCAGACTAAAACTTGAAGTGCGACAGCGCATCCCAATTTAATTTAGTTTAAGGAGTGTCACATTGTCATACAAACATCTAAGTTTTGAAGATCGTACAG
CATTAATGCTTGAGTACCAAAAACCAGATTTTTCTCCTCGAAAATTTGCAAAACAAATTAATCAACATCATAGCGCGATATATCGTGAGTTAAATCGCAA
TTCAACTTCTGAATCATGCCAATCTAAATATGCTATTCAATATTCTTTATTAAGGCGTAGACGTGAGCATCATAAAATAAAACTTGATTCAATCCTATGG
AGATTTATGGTTCAAGCTCTCCGCTGTTTATGGTCTCATGAACAAATCTCTAAACGTTTAAAACAATTATCTGAGTCAAATGGTCATATGTGCATCAGTC
ATACAACGATTTATTCAACCATTCGAGCTTTACCTAAAGGTGAATTGAAGAAAGACTTGCTCTCTTGTTTGAGGCATAAAAATAAAAAACCTAAGGCGAA
TGGTGAACCTAAAAAAGATTCAATATTACAAGAAATTAAAACGATACATGAACGCCCACAGGATGTGGAAGAAAGATACCTGGACATTGGGAAGCTGATT
TAATTAAAGGCAAGGACAATAAGAGTTCGATTGCTACTCTGATTGAGCGCAATACTCGGCTTTGCATATTGGCAGTATTACCTGATGCAAAAGCAGAATC
TGTACGCTTGGCATTAACGAAGGCTTTGAAATATCTTCCAGATGAATTACGTAAAACACTGACATATGACCGAGGTCGAGAAATGGCAGAGCATAAAATT
TTAGAACAGGATTTAGGGATGGATGTATATTTTTGTGATCCGCATTCACCCTGGCAGAAAGGGACTTGTGAAAATATGAATGGTCTAATTCGTCAATATT
TACCTAAAGGTATTGACCTGAATCAAGCAGATCAGCATTATTTGAATCAAGTCGCTATGTCCCTCAATACAAGACCAACAAAGGCTTTAGATTGGTATAC
TCCGTTAGAGAAATTTACCCAATTAGTTGATTATCATCAGGCTTTTAAATCTGTCACACTTCATGTTTGAATACGCT

Recoding section

Recoding by frameshift
Frame -1
Type translational
Experimentally demonstrated No

Stimulators :

Shine-Dalgarno sequence : No
Secondary structure : No-structure

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
498 bp	162 aa	106	603	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MLEYQKPDFSPRKFAKQINQHHSAIYRELNRNSTSESCQSKYAIQYSLLRRRREHHKIKLDSILWRFMVQALRCLWSHEQISKRLKQLSESNGHMCISHT
TIYSTIRALPKGELKKDLLSCLRHKNKKPKANGEPKKDSILQEIKTIHERPQDVEERYLDIGKLI

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
519 bp	172 aa	552	1070	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

TPTGCGRKIPGHWEADLIKGKDNKSSIATLIERNTRLCILAVLPDAKAESVRLALTKALKYLPDELRKTLTYDRGREMAEHKILEQDLGMDVYFCDPHSP
WQKGTCENMNGLIRQYLPKGIDLNQADQHYLNQVAMSLNTRPTKALDWYTPLEKFTQLVDYHQAFKSVTLHV

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
965 bp	322 aa	106	1070	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLEYQKPDFSPRKFAKQINQHHSAIYRELNRNSTSESCQSKYAIQYSLLRRRREHHKIKLDSILWRFMVQALRCLWSHEQISKRLKQLSESNGHMCISHT
TIYSTIRALPKGELKKDLLSCLRHKNKKPKANGEPKKDSILQEIKTIHERPQDVEERKIPGHWEADLIKGKDNKSSIATLIERNTRLCILAVLPDAKAES
VRLALTKALKYLPDELRKTLTYDRGREMAEHKILEQDLGMDVYFCDPHSPWQKGTCENMNGLIRQYLPKGIDLNQADQHYLNQVAMSLNTRPTKALDWYT
PLEKFTQLVDYHQAFKSVTLHV

Blast result :

Comments

ISAbe8 is 88% aa similar to ISAba125.
The third is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.

References

1] Brovedan M.A. (2018) Direct submission.
2] Brovedan,M., Marchiaro,P.M., Moran-Barrio,J., Revale,S., Cameranesi,M., Brambilla,L., Viale,A.M. and Limansky,A.S. (2016) Genome Announc 4 (2).