ISAbe6
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Acinetobacter bereziniae | Acinetobacter bereziniae HPC229 |
DNA section
IS Length : 1197 bp
Ends
IR Length : 28/40
IRL : GGTAATCCCCCCTCAAAATAATTGAACTCAAAAGTAGAAAATCGAATAAT
IRR : GGTAATACTCCCATTTTTAATTGATTTTATAAGTAGAAAGGCAAAGTCCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ttccttta | atgg | aatttgtg | 4 |
DNA sequence
GGTAATCCCCCCTCAAAATAATTGAACTCAAAAGTAGAAAATCGAATAATCCTGTAAAGGAGATTTTTTATGAAAACATCTAAATATTCAGACAGCTTAA
TCATGAGTATTTTAAAACAAGCTGAATCAGGAACACCCGTGCCTAACTTATGCCGTGAACACGGAATGAGTTCCGCTACATTTTATAAATGGAAAGCGAA
ATATGGAGGTATGGATATTTCAATGATGACACGACTTAAAGAATTAGAAGCTGAAAATGCTCGTCTGAAACGTATGTATGCTGACGAACGTCTTAAAGCA
GATATTCTTCAGGATGCACTGTCAAAGAAGTTATAAAGCCTGTACAACGTAAATCTCTTGCTCAGCGAGCCGTTGAACAATTCAAGATTTCAGTGAGTAA
AGCTTGCTTCATCTTTCAACTTAGTCAAACCTGTTATCGTTATAAAGCTCAATTAGATGAGCAAAATAAGATGATTGCAGACTGGCTACTGAAGCTCACC
AACGAGAATAAGACTTGGGGCTTTCAGCTTTGCTTCTTGTACCTACGTAATGTCAAAGGCTATTCATGGAACCATAAACGTGTTTACCGAATTTATAAGG
AGCTTGAGCTTAACCTACGAATTAAACCAAACAAACGCATTCAGCGAATAAAGCCTGAACCGCTTACAGTCCCATCAACACCAAATGAATCGTGGTCAAT
GGACTTTATGCATGACCAACTGAGTGATGGTCGCAGTTACCGAATTCACAATGTTATTGATGATTATAACCGTGAGTCACTGGATATATTGATTGATTTT
TCTTTGCCAGCTCAACGAGTGCTGAGGGGATTGGATCAATTAATTGCATGGCGAGGTAAGCCAAAGCGGATCCGTAGTGATAATGGACCCGAGTATGTTA
GTGATTTGATGGAAGCATGGTGTACTCAACATGGAATTGAGCATGTATTTACTCAACCTGGTCATCCACAACAGAATGCTTATGTAGAACGCTTCAACAG
GACGGTGAGATATGAATGTTTGAATCAATATTTATTTAGAGAGCTTGAAGAAGTTGAAGACTATACAACGATATGGCAACACTTTTATAATCATGAACGA
CCACATATGTCAGTTGATGGTAAACCACCTAAATTTAAACAATGATAAGGACTTTGCCTTTCTACTTATAAAATCAATTAAAAATGGGAGTATTACC
TCATGAGTATTTTAAAACAAGCTGAATCAGGAACACCCGTGCCTAACTTATGCCGTGAACACGGAATGAGTTCCGCTACATTTTATAAATGGAAAGCGAA
ATATGGAGGTATGGATATTTCAATGATGACACGACTTAAAGAATTAGAAGCTGAAAATGCTCGTCTGAAACGTATGTATGCTGACGAACGTCTTAAAGCA
GATATTCTTCAGGATGCACTGTCAAAGAAGTTATAAAGCCTGTACAACGTAAATCTCTTGCTCAGCGAGCCGTTGAACAATTCAAGATTTCAGTGAGTAA
AGCTTGCTTCATCTTTCAACTTAGTCAAACCTGTTATCGTTATAAAGCTCAATTAGATGAGCAAAATAAGATGATTGCAGACTGGCTACTGAAGCTCACC
AACGAGAATAAGACTTGGGGCTTTCAGCTTTGCTTCTTGTACCTACGTAATGTCAAAGGCTATTCATGGAACCATAAACGTGTTTACCGAATTTATAAGG
AGCTTGAGCTTAACCTACGAATTAAACCAAACAAACGCATTCAGCGAATAAAGCCTGAACCGCTTACAGTCCCATCAACACCAAATGAATCGTGGTCAAT
GGACTTTATGCATGACCAACTGAGTGATGGTCGCAGTTACCGAATTCACAATGTTATTGATGATTATAACCGTGAGTCACTGGATATATTGATTGATTTT
TCTTTGCCAGCTCAACGAGTGCTGAGGGGATTGGATCAATTAATTGCATGGCGAGGTAAGCCAAAGCGGATCCGTAGTGATAATGGACCCGAGTATGTTA
GTGATTTGATGGAAGCATGGTGTACTCAACATGGAATTGAGCATGTATTTACTCAACCTGGTCATCCACAACAGAATGCTTATGTAGAACGCTTCAACAG
GACGGTGAGATATGAATGTTTGAATCAATATTTATTTAGAGAGCTTGAAGAAGTTGAAGACTATACAACGATATGGCAACACTTTTATAATCATGAACGA
CCACATATGTCAGTTGATGGTAAACCACCTAAATTTAAACAATGATAAGGACTTTGCCTTTCTACTTATAAAATCAATTAAAAATGGGAGTATTACC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 70 | 336 | + | No |
Description : First part of the transposase
ORF sequence :
MKTSKYSDSLIMSILKQAESGTPVPNLCREHGMSSATFYKWKAKYGGMDISMMTRLKELEAENARLKRMYADERLKADILQDALSKKL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 849 bp | 282 aa | 297 | 1145 | + | No |
Description : Second part of the transposase
ORF sequence :
SRYSSGCTVKEVIKPVQRKSLAQRAVEQFKISVSKACFIFQLSQTCYRYKAQLDEQNKMIADWLLKLTNENKTWGFQLCFLYLRNVKGYSWNHKRVYRIY
KELELNLRIKPNKRIQRIKPEPLTVPSTPNESWSMDFMHDQLSDGRSYRIHNVIDDYNRESLDILIDFSLPAQRVLRGLDQLIAWRGKPKRIRSDNGPEY
VSDLMEAWCTQHGIEHVFTQPGHPQQNAYVERFNRTVRYECLNQYLFRELEEVEDYTTIWQHFYNHERPHMSVDGKPPKFKQ
KELELNLRIKPNKRIQRIKPEPLTVPSTPNESWSMDFMHDQLSDGRSYRIHNVIDDYNRESLDILIDFSLPAQRVLRGLDQLIAWRGKPKRIRSDNGPEY
VSDLMEAWCTQHGIEHVFTQPGHPQQNAYVERFNRTVRYECLNQYLFRELEEVEDYTTIWQHFYNHERPHMSVDGKPPKFKQ
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1076 bp | 358 aa | 70 | 1145 | + | Yes |
Chemistry : DDE
ORF sequence :
MKTSKYSDSLIMSILKQAESGTPVPNLCREHGMSSATFYKWKAKYGGMDISMMTRLKELEAENARLKRMYADERLKADILQDALSKKVIKPVQRKSLAQR
AVEQFKISVSKACFIFQLSQTCYRYKAQLDEQNKMIADWLLKLTNENKTWGFQLCFLYLRNVKGYSWNHKRVYRIYKELELNLRIKPNKRIQRIKPEPLT
VPSTPNESWSMDFMHDQLSDGRSYRIHNVIDDYNRESLDILIDFSLPAQRVLRGLDQLIAWRGKPKRIRSDNGPEYVSDLMEAWCTQHGIEHVFTQPGHP
QQNAYVERFNRTVRYECLNQYLFRELEEVEDYTTIWQHFYNHERPHMSVDGKPPKFKQ
AVEQFKISVSKACFIFQLSQTCYRYKAQLDEQNKMIADWLLKLTNENKTWGFQLCFLYLRNVKGYSWNHKRVYRIYKELELNLRIKPNKRIQRIKPEPLT
VPSTPNESWSMDFMHDQLSDGRSYRIHNVIDDYNRESLDILIDFSLPAQRVLRGLDQLIAWRGKPKRIRSDNGPEYVSDLMEAWCTQHGIEHVFTQPGHP
QQNAYVERFNRTVRYECLNQYLFRELEEVEDYTTIWQHFYNHERPHMSVDGKPPKFKQ
Blast result :
Comments
ISAbe6 is 95% (transposase) aa similar to ISAba20.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] Brovedan M.A. (2018) Direct submission.