ISEfa15
- Family IS21
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| MF580438 | ND | Enterococcus faecium | Enterococcus faecium Enterococcus faecium E35048 plasmid pE35048-oc |
DNA section
IS Length : 2500 bp
Ends
IR Length : 39/50
IRL : TGTTTATGATAAGCTAAAAGTACCCCAAATTCTAATTGAAAATTCCCCTA
IRR : TGTATTTGTCAAGCGAAATTTCCCCCATATTCTAATCGAAAACTCCCCTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTTTAAACAACAGCACC | CTAAT | TTTGATGAACTAGTGAA | 5 |
DNA sequence
TGTTTATGATAAGCTAAAAGTACCCCAAATTCTAATTGAAAATTCCCCTAGAGGAAATATATAATATCCCTATGACAACTCATAGGGGGGACTAGGGGAT
GATAAGTTTAATGGATAAACAGAAGATAATAATAGATGGATTTTTAAATGGAAAATCTCAATGGGGAATCCATAGGGAAACAGGAATTTCTAGAAAAACA
ATTAGGAAATATATTAGGGAGTATGAAGAGAAGAGATCCAAATTATTAGAAGGAGAAGGTGATAAATTAATACTTACTGAAGAGATGATAGAGCCGCCAA
AATATGATTCTAGTAACAGACAAAAGGTAAAGTTAACAGATGAAATAATGGCTAGGATAGATTTTTATCTAGAGGAAAATGAAGAAAAAAGAAGGATCGG
CAAGAGTAAACAACAAAAGAAAAAAATAGATATACATGAGTGTTTAGTAGAAGAAGGCTACGACATAAGTTATCCGACAGTATGTAACTATATAAGGAAT
ATATCAGATGAAAAAAAGGAGGCCTACATCAGGCAAGAATATGAATTAGGAGATGTATCTGAATTTGATTGGGGCCATGTTAAATTAGAAATAAATGGGG
AGTCTAAAAACATACAAATGGCTGTAATGACAACAGCTAAGGGTAACTACAGGTTTTCGTATCTTTATCAAAATCAGAAGATGGAGAGTTTCCTAGATTC
ACATGTCAGATTTTTTAATCATGTGGGAGGTGTTCATAGGGAAATAGTATATGACAATATGAAGGTTGCAGTTAAGAGATTTGTAACAAAGGCTGATAAG
GAACCAACTGATGGACTTTTAAAACTATCCATGTATTATGGATTTAACTATAGATTTTGCAATGCTAGACGTGGAAATGAAAAAGGTCATGTGGAAAGGT
CAGTAGAATATGTAAGAAGAAAGGTATTTAGTAAAAAGGATACATTTGAAACACTAGAGGAGGCCAATAAGTATTTAGAAGAGGAACTGAATAAATTAAA
TTCAAAACCGCAGAAGTACAATGAAAACAAAACAGCAAAGGATATATTAATAGAGGAATTCCCCTACCTAATAAAACTTATGCCTACTTATGATATTGCA
AGGGTGGTGGAATTAAGGGTAAATAAATATTCAGTAATTTCAATAGATGAAAATAAATACTCAGTACCTGATTCTCTAGTCGGTAAATTTGTAACAGCAA
AAATATATCCAAATAATATAACAATCTACCATGAAAACATAAGGGTAACTGAGCATAAGAGGAGTTTTGGATTAAGTACATGGAATATAAAAATAAAACA
TTACCTAAATACATTAAAAAAGAAGCCTGGGGCAATTCATAACAGCACAGCCATGCGTCAAATGAATTCCAAGCTTCAAAACATATACAATAAATATTAT
ACCCAAAATCCAAGGGATTTCATAGACCTAATTGAATTAATTTCAGAAGAAGGTTTGGAAAGGATAGAAGAGATTATAAAAGAATTAGAAAGGATAAACC
CATTAAATATAGACACGGAAAAAATAAAGCTTCTGTGCCACAGAAAGGATGAATCCATAGGGGAAAAGACATACAAAAATACAGAGATAGAAAAACATTC
AAAGCTCATCCTAAATCACTATGGAGGATTACTAAAAGGTTCACCTGTAGAATTTGATAAGGAGGCTTTGATAATATGAAAAAGGATGCAATTAAAATAA
ATATTAAAAACTTAGCAAAGGAGTTAAAATTACCATATATAAGTAAAAGCATAGAAGAAGAAATAAAAGAAGCAAATATGAAGAATTTAACATACGAAGA
TTTTATCTTGTCGGTCCTGGAAAATGAACATGATTTAAGAAAATCTAATGGAATAAAAAGTAGGATTAGGCAAGCAAGATTTCCATATAAAAAATATATA
GAGGATTTAAGTTTAGAGGATTTACCCCAAGATGCAAGAGATAAATTAAAAATCTTTTCATCCTTAGAGTTCATAGAAACAGGCCAAAATATAATACTTG
CAGGTAACCCTGGAACTGGGAAGACGCATATGGCCATAGGTATTGGTATTAAGGCTTGTACTAAGGGCTACAAGGTTCTATTTACAACAATACCACTACT
TGTAAATGAATTAAAAGAAAGCAGATCAGAAAAAACATTAAGGGCTTTTGAAAGAAGATTTGAAAAATATGATCTAATAATAGCAGATGAATTAGGGTAT
ATATCTTTTGATAAAGAGGCTTCAGAACTACTTTTTACCTACCTCTCACTAAGAGCAGGAAGGAAATCAACAATAATAACTACCAATCTATCATTTGAAA
GGTGGGATGAAATATTTAAAGATCCAGTAATGACAGCAGCAATGATAGACAGGCTTACCCATAAATCATATATAGTGAATATGAATGGCAATTCTTATAG
ATTAAAAGAGACCCAGCTTTGGTTAGAAGGGCAATAATTTTTTATCCAGCTAGGGGAGTTTTCGATTAGAATATGGGGGAAATTTCGCTTGACAAATACA
GATAAGTTTAATGGATAAACAGAAGATAATAATAGATGGATTTTTAAATGGAAAATCTCAATGGGGAATCCATAGGGAAACAGGAATTTCTAGAAAAACA
ATTAGGAAATATATTAGGGAGTATGAAGAGAAGAGATCCAAATTATTAGAAGGAGAAGGTGATAAATTAATACTTACTGAAGAGATGATAGAGCCGCCAA
AATATGATTCTAGTAACAGACAAAAGGTAAAGTTAACAGATGAAATAATGGCTAGGATAGATTTTTATCTAGAGGAAAATGAAGAAAAAAGAAGGATCGG
CAAGAGTAAACAACAAAAGAAAAAAATAGATATACATGAGTGTTTAGTAGAAGAAGGCTACGACATAAGTTATCCGACAGTATGTAACTATATAAGGAAT
ATATCAGATGAAAAAAAGGAGGCCTACATCAGGCAAGAATATGAATTAGGAGATGTATCTGAATTTGATTGGGGCCATGTTAAATTAGAAATAAATGGGG
AGTCTAAAAACATACAAATGGCTGTAATGACAACAGCTAAGGGTAACTACAGGTTTTCGTATCTTTATCAAAATCAGAAGATGGAGAGTTTCCTAGATTC
ACATGTCAGATTTTTTAATCATGTGGGAGGTGTTCATAGGGAAATAGTATATGACAATATGAAGGTTGCAGTTAAGAGATTTGTAACAAAGGCTGATAAG
GAACCAACTGATGGACTTTTAAAACTATCCATGTATTATGGATTTAACTATAGATTTTGCAATGCTAGACGTGGAAATGAAAAAGGTCATGTGGAAAGGT
CAGTAGAATATGTAAGAAGAAAGGTATTTAGTAAAAAGGATACATTTGAAACACTAGAGGAGGCCAATAAGTATTTAGAAGAGGAACTGAATAAATTAAA
TTCAAAACCGCAGAAGTACAATGAAAACAAAACAGCAAAGGATATATTAATAGAGGAATTCCCCTACCTAATAAAACTTATGCCTACTTATGATATTGCA
AGGGTGGTGGAATTAAGGGTAAATAAATATTCAGTAATTTCAATAGATGAAAATAAATACTCAGTACCTGATTCTCTAGTCGGTAAATTTGTAACAGCAA
AAATATATCCAAATAATATAACAATCTACCATGAAAACATAAGGGTAACTGAGCATAAGAGGAGTTTTGGATTAAGTACATGGAATATAAAAATAAAACA
TTACCTAAATACATTAAAAAAGAAGCCTGGGGCAATTCATAACAGCACAGCCATGCGTCAAATGAATTCCAAGCTTCAAAACATATACAATAAATATTAT
ACCCAAAATCCAAGGGATTTCATAGACCTAATTGAATTAATTTCAGAAGAAGGTTTGGAAAGGATAGAAGAGATTATAAAAGAATTAGAAAGGATAAACC
CATTAAATATAGACACGGAAAAAATAAAGCTTCTGTGCCACAGAAAGGATGAATCCATAGGGGAAAAGACATACAAAAATACAGAGATAGAAAAACATTC
AAAGCTCATCCTAAATCACTATGGAGGATTACTAAAAGGTTCACCTGTAGAATTTGATAAGGAGGCTTTGATAATATGAAAAAGGATGCAATTAAAATAA
ATATTAAAAACTTAGCAAAGGAGTTAAAATTACCATATATAAGTAAAAGCATAGAAGAAGAAATAAAAGAAGCAAATATGAAGAATTTAACATACGAAGA
TTTTATCTTGTCGGTCCTGGAAAATGAACATGATTTAAGAAAATCTAATGGAATAAAAAGTAGGATTAGGCAAGCAAGATTTCCATATAAAAAATATATA
GAGGATTTAAGTTTAGAGGATTTACCCCAAGATGCAAGAGATAAATTAAAAATCTTTTCATCCTTAGAGTTCATAGAAACAGGCCAAAATATAATACTTG
CAGGTAACCCTGGAACTGGGAAGACGCATATGGCCATAGGTATTGGTATTAAGGCTTGTACTAAGGGCTACAAGGTTCTATTTACAACAATACCACTACT
TGTAAATGAATTAAAAGAAAGCAGATCAGAAAAAACATTAAGGGCTTTTGAAAGAAGATTTGAAAAATATGATCTAATAATAGCAGATGAATTAGGGTAT
ATATCTTTTGATAAAGAGGCTTCAGAACTACTTTTTACCTACCTCTCACTAAGAGCAGGAAGGAAATCAACAATAATAACTACCAATCTATCATTTGAAA
GGTGGGATGAAATATTTAAAGATCCAGTAATGACAGCAGCAATGATAGACAGGCTTACCCATAAATCATATATAGTGAATATGAATGGCAATTCTTATAG
ATTAAAAGAGACCCAGCTTTGGTTAGAAGGGCAATAATTTTTTATCCAGCTAGGGGAGTTTTCGATTAGAATATGGGGGAAATTTCGCTTGACAAATACA
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1581 bp | 526 aa | 99 | 1679 | + | No |
Chemistry : DDE
ORF sequence :
MISLMDKQKIIIDGFLNGKSQWGIHRETGISRKTIRKYIREYEEKRSKLLEGEGDKLILTEEMIEPPKYDSSNRQKVKLTDEIMARIDFYLEENEEKRRI
GKSKQQKKKIDIHECLVEEGYDISYPTVCNYIRNISDEKKEAYIRQEYELGDVSEFDWGHVKLEINGESKNIQMAVMTTAKGNYRFSYLYQNQKMESFLD
SHVRFFNHVGGVHREIVYDNMKVAVKRFVTKADKEPTDGLLKLSMYYGFNYRFCNARRGNEKGHVERSVEYVRRKVFSKKDTFETLEEANKYLEEELNKL
NSKPQKYNENKTAKDILIEEFPYLIKLMPTYDIARVVELRVNKYSVISIDENKYSVPDSLVGKFVTAKIYPNNITIYHENIRVTEHKRSFGLSTWNIKIK
HYLNTLKKKPGAIHNSTAMRQMNSKLQNIYNKYYTQNPRDFIDLIELISEEGLERIEEIIKELERINPLNIDTEKIKLLCHRKDESIGEKTYKNTEIEKH
SKLILNHYGGLLKGSPVEFDKEALII
GKSKQQKKKIDIHECLVEEGYDISYPTVCNYIRNISDEKKEAYIRQEYELGDVSEFDWGHVKLEINGESKNIQMAVMTTAKGNYRFSYLYQNQKMESFLD
SHVRFFNHVGGVHREIVYDNMKVAVKRFVTKADKEPTDGLLKLSMYYGFNYRFCNARRGNEKGHVERSVEYVRRKVFSKKDTFETLEEANKYLEEELNKL
NSKPQKYNENKTAKDILIEEFPYLIKLMPTYDIARVVELRVNKYSVISIDENKYSVPDSLVGKFVTAKIYPNNITIYHENIRVTEHKRSFGLSTWNIKIK
HYLNTLKKKPGAIHNSTAMRQMNSKLQNIYNKYYTQNPRDFIDLIELISEEGLERIEEIIKELERINPLNIDTEKIKLLCHRKDESIGEKTYKNTEIEKH
SKLILNHYGGLLKGSPVEFDKEALII
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 762 bp | 253 aa | 1676 | 2437 | + | No |
AG : IS21 helper
ORF sequence :
MKKDAIKINIKNLAKELKLPYISKSIEEEIKEANMKNLTYEDFILSVLENEHDLRKSNGIKSRIRQARFPYKKYIEDLSLEDLPQDARDKLKIFSSLEFI
ETGQNIILAGNPGTGKTHMAIGIGIKACTKGYKVLFTTIPLLVNELKESRSEKTLRAFERRFEKYDLIIADELGYISFDKEASELLFTYLSLRAGRKSTI
ITTNLSFERWDEIFKDPVMTAAMIDRLTHKSYIVNMNGNSYRLKETQLWLEGQ
ETGQNIILAGNPGTGKTHMAIGIGIKACTKGYKVLFTTIPLLVNELKESRSEKTLRAFERRFEKYDLIIADELGYISFDKEASELLFTYLSLRAGRKSTI
ITTNLSFERWDEIFKDPVMTAAMIDRLTHKSYIVNMNGNSYRLKETQLWLEGQ
Blast result :
Comments
ISEfa15 is 69% (transposase) aa similar to ISHahy12.
As a putative composite transposon found for the first time on plasmid pE35048-oc in Enterococcus faecium E35048, two ISEfa15 elements (one copy of which appear to be trucated at its 3'-end) in the same orientation flank the optrA resistance gene. This composite transposon may play a role in dissemination of the optrA resistance gene, as the presence of target site duplications (5'-CTAAT-3') flanking this transposon strongly suggests its transposition in a pRE25-like plasmid as F120805. Part of this transposon is able to exciss and form minicircles containing a truncated copy of ISEfa15 and the optrA gene.
As a putative composite transposon found for the first time on plasmid pE35048-oc in Enterococcus faecium E35048, two ISEfa15 elements (one copy of which appear to be trucated at its 3'-end) in the same orientation flank the optrA resistance gene. This composite transposon may play a role in dissemination of the optrA resistance gene, as the presence of target site duplications (5'-CTAAT-3') flanking this transposon strongly suggests its transposition in a pRE25-like plasmid as F120805. Part of this transposon is able to exciss and form minicircles containing a truncated copy of ISEfa15 and the optrA gene.
References