ISEfa9
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| EU999035 | ND | Enterococcus faecium | Enterococcus faecium BM4653 |
DNA section
IS Length : 1366 bp
Ends
IR Length : 35/41
IRL : TGATGTTAGAATATAAATAGTACAAAATAAACATAGACCATTTTCGATAA
IRR : TGGTCCTATGTCAAGAAAAAGTACAAATTAAATGTAACCAATTTTCTCTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TGATGTTAGAATATAAATAGTACAAAATAAACATAGACCATTTTCGATAAATAGTATATGATAGTCATAGATCAAGGAGGAGAATGGTTATGGCAAAAGG
TACAAAATATTCCCAACAGTTTAAAGAGGATGCAGTACAGTATCGCTTGGATCATCCGGAGCTGCCACTTCGCAAAGCTGCGGAGAATCTTGGAGTCAGC
GAATCTGCACTGAAAAACTGGATGAAAGTAGCAAAGGAACATAAAGGAGAGGTTCCTACCCGTGGTTCTGGAAATTATGCAAGTGAGGAAGCAAAAGAGA
TTGCCAGATTGCAGCGTGAATTACGAGATACGAAGGATGCTCTTGAAGTGTTAAAAAAAGCAATCGGTATTCTGGGAAACTGACAAAAGCTCTTTACACG
GCTACTTCTGAGTACGTGAAAGAAATCAAATCCCTGCCCGTGAAACGTCAGGTTTCTGTCAGCGGAATACTGAAAAAATTAGGCGTTTCACGTTCGGGTT
ACAATGCATGGAAGAAACGGGTTCCTTCGGACACGTCTGTTCGCCGTGCAGTTTTAAAAGAAAAAATTCAAAAGATCTACGAGGAATCCCATCAAAACTA
CGGTGCTCCTAAAATTACTGCAGAATTGCGAAAATCCGGGGAGTATGTTTCCGAAAAAACAGTTGGAAATTATATGCGTCAAATGGGAATCAGAGCCCAT
TGGGTGAAACCATATATCCAGACAACCATAGATTCTGATCTCAGCCAGAAACTCAAAAATATCCTCAACGAGGAATGCAATCCTGCTCATCCAGATGCTG
TCTGGTGTACAGATATTACTTATATTTGGACATTTGAAGGATTTGTGTATCTTACCAGTGTGATGGATCTGTATTCAAGAAAAATCATTTCCTGGGTGTT
GAGTGAGACGCTGGAGGCCTCCCACGTAGTGGAATGTGTTGAAAAAGCAAAACGTGTCAGAAATGTTGAAAAACCGTTGATCTTCCACTGCGACAGAGGA
TGCCAGTACGTATCGGAAGCATTTCAAAAAGCAACCAAAGGTATGATTCACAGTTATTCAAAGAAAGCATATCCATGGGATAACGCCTGTATAGAATCTT
TCCATGCACTTATGAAGAGAGAATGGATCAACCGGTTTAAAATCTTTAATTATGCACATGCACATAAATTGATTTTTGAATACATCGAAACATTTTACAA
TACCGTGCGCATTCACAGTCATTGTGGATATTTGTCTCCAAATGAATATGAGGAGCAATATCTTGAAAACATCGAGGAGAATGCAATAAAAATAGCAAGT
TAAACTTCGCTCAGAACAGAGAAAATTGGTTACATTTAATTTGTACTTTTTCTTGACATAGGACCA
TACAAAATATTCCCAACAGTTTAAAGAGGATGCAGTACAGTATCGCTTGGATCATCCGGAGCTGCCACTTCGCAAAGCTGCGGAGAATCTTGGAGTCAGC
GAATCTGCACTGAAAAACTGGATGAAAGTAGCAAAGGAACATAAAGGAGAGGTTCCTACCCGTGGTTCTGGAAATTATGCAAGTGAGGAAGCAAAAGAGA
TTGCCAGATTGCAGCGTGAATTACGAGATACGAAGGATGCTCTTGAAGTGTTAAAAAAAGCAATCGGTATTCTGGGAAACTGACAAAAGCTCTTTACACG
GCTACTTCTGAGTACGTGAAAGAAATCAAATCCCTGCCCGTGAAACGTCAGGTTTCTGTCAGCGGAATACTGAAAAAATTAGGCGTTTCACGTTCGGGTT
ACAATGCATGGAAGAAACGGGTTCCTTCGGACACGTCTGTTCGCCGTGCAGTTTTAAAAGAAAAAATTCAAAAGATCTACGAGGAATCCCATCAAAACTA
CGGTGCTCCTAAAATTACTGCAGAATTGCGAAAATCCGGGGAGTATGTTTCCGAAAAAACAGTTGGAAATTATATGCGTCAAATGGGAATCAGAGCCCAT
TGGGTGAAACCATATATCCAGACAACCATAGATTCTGATCTCAGCCAGAAACTCAAAAATATCCTCAACGAGGAATGCAATCCTGCTCATCCAGATGCTG
TCTGGTGTACAGATATTACTTATATTTGGACATTTGAAGGATTTGTGTATCTTACCAGTGTGATGGATCTGTATTCAAGAAAAATCATTTCCTGGGTGTT
GAGTGAGACGCTGGAGGCCTCCCACGTAGTGGAATGTGTTGAAAAAGCAAAACGTGTCAGAAATGTTGAAAAACCGTTGATCTTCCACTGCGACAGAGGA
TGCCAGTACGTATCGGAAGCATTTCAAAAAGCAACCAAAGGTATGATTCACAGTTATTCAAAGAAAGCATATCCATGGGATAACGCCTGTATAGAATCTT
TCCATGCACTTATGAAGAGAGAATGGATCAACCGGTTTAAAATCTTTAATTATGCACATGCACATAAATTGATTTTTGAATACATCGAAACATTTTACAA
TACCGTGCGCATTCACAGTCATTGTGGATATTTGTCTCCAAATGAATATGAGGAGCAATATCTTGAAAACATCGAGGAGAATGCAATAAAAATAGCAAGT
TAAACTTCGCTCAGAACAGAGAAAATTGGTTACATTTAATTTGTACTTTTTCTTGACATAGGACCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 294 bp | 97 aa | 90 | 383 | + | No |
Description : First part of the transposase
ORF sequence :
MAKGTKYSQQFKEDAVQYRLDHPELPLRKAAENLGVSESALKNWMKVAKEHKGEVPTRGSGNYASEEAKEIARLQRELRDTKDALEVLKKAIGILGN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 957 bp | 318 aa | 347 | 1303 | + | No |
Description : Second part of the transposase
ORF sequence :
SVKKSNRYSGKLTKALYTATSEYVKEIKSLPVKRQVSVSGILKKLGVSRSGYNAWKKRVPSDTSVRRAVLKEKIQKIYEESHQNYGAPKITAELRKSGEY
VSEKTVGNYMRQMGIRAHWVKPYIQTTIDSDLSQKLKNILNEECNPAHPDAVWCTDITYIWTFEGFVYLTSVMDLYSRKIISWVLSETLEASHVVECVEK
AKRVRNVEKPLIFHCDRGCQYVSEAFQKATKGMIHSYSKKAYPWDNACIESFHALMKREWINRFKIFNYAHAHKLIFEYIETFYNTVRIHSHCGYLSPNE
YEEQYLENIEENAIKIAS
VSEKTVGNYMRQMGIRAHWVKPYIQTTIDSDLSQKLKNILNEECNPAHPDAVWCTDITYIWTFEGFVYLTSVMDLYSRKIISWVLSETLEASHVVECVEK
AKRVRNVEKPLIFHCDRGCQYVSEAFQKATKGMIHSYSKKAYPWDNACIESFHALMKREWINRFKIFNYAHAHKLIFEYIETFYNTVRIHSHCGYLSPNE
YEEQYLENIEENAIKIAS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1214 bp | 404 aa | 90 | 1303 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKGTKYSQQFKEDAVQYRLDHPELPLRKAAENLGVSESALKNWMKVAKEHKGEVPTRGSGNYASEEAKEIARLQRELRDTKDALEVLKKSNRYSGKLTK
ALYTATSEYVKEIKSLPVKRQVSVSGILKKLGVSRSGYNAWKKRVPSDTSVRRAVLKEKIQKIYEESHQNYGAPKITAELRKSGEYVSEKTVGNYMRQMG
IRAHWVKPYIQTTIDSDLSQKLKNILNEECNPAHPDAVWCTDITYIWTFEGFVYLTSVMDLYSRKIISWVLSETLEASHVVECVEKAKRVRNVEKPLIFH
CDRGCQYVSEAFQKATKGMIHSYSKKAYPWDNACIESFHALMKREWINRFKIFNYAHAHKLIFEYIETFYNTVRIHSHCGYLSPNEYEEQYLENIEENAI
KIAS
ALYTATSEYVKEIKSLPVKRQVSVSGILKKLGVSRSGYNAWKKRVPSDTSVRRAVLKEKIQKIYEESHQNYGAPKITAELRKSGEYVSEKTVGNYMRQMG
IRAHWVKPYIQTTIDSDLSQKLKNILNEECNPAHPDAVWCTDITYIWTFEGFVYLTSVMDLYSRKIISWVLSETLEASHVVECVEKAKRVRNVEKPLIFH
CDRGCQYVSEAFQKATKGMIHSYSKKAYPWDNACIESFHALMKREWINRFKIFNYAHAHKLIFEYIETFYNTVRIHSHCGYLSPNEYEEQYLENIEENAI
KIAS
Blast result :
Comments
ISEfa9 is 56% aa similar to ISHahy5.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder submission (2018)
2] Depardieu,F., Foucault,M.L., Bell,J., Dubouix,A., Guibert,M., Lavigne,J.P., Levast,M. and Courvalin,P. (2009) Antimicrob. Agents Chemother. 53 (5), 1952-1963.
2] Depardieu,F., Foucault,M.L., Bell,J., Dubouix,A., Guibert,M., Lavigne,J.P., Levast,M. and Courvalin,P. (2009) Antimicrob. Agents Chemother. 53 (5), 1952-1963.