ISEch2
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP002038 | ND | Dickeya dadantii | Dickeya dadantii 3937 |
DNA section
IS Length : 1444 bp
Ends
IR Length : 19/23
IRL : TGGACTGCACCCCAAAAGTTGGATACCCAACTGAGTAAGGTGCAGTTTTT
IRR : TGGACTGACCCCATAAAGTTGGACAGTTCATGTTAAGCGGCTTTCAAGGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGCCCCGCTT | GTTA | TCGCGGGGCT | 4 |
DNA sequence
TGGACTGCACCCCAAAAGTTGGATACCCAACTGAGTAAGGTGCAGTTTTTTATGGCAACACCCAAGTATTCTCTCGAATTAAGACTCTCAGTCGTAAACC
ACTTTCTCTCGGGCAGGGATGGAACCCGTCGCACTGCCGAAATTTTTGGCGTCGAAAGGACATCAGTTCGACGCTGGGTCAGAGCCTTTCAACTTCATGG
GATTGATGGCATTACCTGGAAAAACGATAGCCATTCTGCAGATTTCAGGCTTATTGTTGTGCAGGCCGTTCTCAACGAAGAGCTTTCAATGCGTGAAGCT
GCGGCCCGCTTTAATATCTCAAATGAAACGGTTGTTCGTCGCTGGGTCTCCGTGTACAACGATGCGGGACCGGAAGTACTCCGGAATATGAAACGCGGAA
GGCCCCGGGAAATGACCAGAAAAACGACACCCCCTCCTGTTGATGCAGTGCTGGAACAGCTGTCTCCTGAAGAACTCAGGGCTGAGCTTCGCTATCTGCG
GGCGGAGAATGCCTATCTAAAAAAGCTGAAAGCCTTGGTTCAAAGCGAAAAAGACGGCAAAAAGCCCAAATAATCAGTGAACTAAGGCAAGGGCATGCCC
TCAATGACCTTCTGCGTGCAGCGGGCATGTCCCGAAGTACGTGGTATCACAACATCAGCGCGATGAAGCGAACGGATAAACATGCTGGGCTGAAAGCCAG
AATCGGCGACATTTACCACCATCATAAAGGGCGTTACGGATATCGCCGGATCACACTCTCGCTGAGAAAAGAGGGGATCGCGGTGAATCATAAGACGGTG
CAGCGCCTGATGGTGGAACTGTCGCTCCGCTCGCTGATTAGAGTGAAGAAATATCGCTCGTGGAAGGGAGAAACAGGCAAAGCAGCACCTAACATCCTTA
ACCGAAACTTCTGCGCGTCAAAATCCAACGAAAAGTGGGTTACAGATGTTACCGAGTTCTCGTTGCAGGGTAAGAAGTTGTACCTGTCTCCGGTTCTCGA
TCTGTTTAACAGGGAAATTATCTCCTACAGCCTGTCCGAAAGACCGGTCATGGATATGGTGAATACCATGCTGAGTGATGCGTTATCAGTGCTCAGTCCG
GAAGAGAGTCCGCTGCTGCACTCGGATCAGGGCTGGCAGTACCGTATGGCAGGTTATCAGGAAAAGTTAAAAGCAAAGGGCGTGGTGCAAAGTATGTCAC
GCAAAGGAAACTGTCTGGATAATGCCGTGATAGAGAACTTCTTTGGCACGTTGAAATCGGAGTGTTTTTATCCTGGTCAGTTCTGCAGCATTGAAGAGCT
GAAGCCGGCGATACAGGACTATATCCACTACTACAATAACGAGCGGATAAGCCTGAAGCTGAAAGGCCTGAGTCCGGTTGAATACCGAACCCAGGCCTTG
AAAGCCGCTTAACATGAACTGTCCAACTTTATGGGGTCAGTCCA
ACTTTCTCTCGGGCAGGGATGGAACCCGTCGCACTGCCGAAATTTTTGGCGTCGAAAGGACATCAGTTCGACGCTGGGTCAGAGCCTTTCAACTTCATGG
GATTGATGGCATTACCTGGAAAAACGATAGCCATTCTGCAGATTTCAGGCTTATTGTTGTGCAGGCCGTTCTCAACGAAGAGCTTTCAATGCGTGAAGCT
GCGGCCCGCTTTAATATCTCAAATGAAACGGTTGTTCGTCGCTGGGTCTCCGTGTACAACGATGCGGGACCGGAAGTACTCCGGAATATGAAACGCGGAA
GGCCCCGGGAAATGACCAGAAAAACGACACCCCCTCCTGTTGATGCAGTGCTGGAACAGCTGTCTCCTGAAGAACTCAGGGCTGAGCTTCGCTATCTGCG
GGCGGAGAATGCCTATCTAAAAAAGCTGAAAGCCTTGGTTCAAAGCGAAAAAGACGGCAAAAAGCCCAAATAATCAGTGAACTAAGGCAAGGGCATGCCC
TCAATGACCTTCTGCGTGCAGCGGGCATGTCCCGAAGTACGTGGTATCACAACATCAGCGCGATGAAGCGAACGGATAAACATGCTGGGCTGAAAGCCAG
AATCGGCGACATTTACCACCATCATAAAGGGCGTTACGGATATCGCCGGATCACACTCTCGCTGAGAAAAGAGGGGATCGCGGTGAATCATAAGACGGTG
CAGCGCCTGATGGTGGAACTGTCGCTCCGCTCGCTGATTAGAGTGAAGAAATATCGCTCGTGGAAGGGAGAAACAGGCAAAGCAGCACCTAACATCCTTA
ACCGAAACTTCTGCGCGTCAAAATCCAACGAAAAGTGGGTTACAGATGTTACCGAGTTCTCGTTGCAGGGTAAGAAGTTGTACCTGTCTCCGGTTCTCGA
TCTGTTTAACAGGGAAATTATCTCCTACAGCCTGTCCGAAAGACCGGTCATGGATATGGTGAATACCATGCTGAGTGATGCGTTATCAGTGCTCAGTCCG
GAAGAGAGTCCGCTGCTGCACTCGGATCAGGGCTGGCAGTACCGTATGGCAGGTTATCAGGAAAAGTTAAAAGCAAAGGGCGTGGTGCAAAGTATGTCAC
GCAAAGGAAACTGTCTGGATAATGCCGTGATAGAGAACTTCTTTGGCACGTTGAAATCGGAGTGTTTTTATCCTGGTCAGTTCTGCAGCATTGAAGAGCT
GAAGCCGGCGATACAGGACTATATCCACTACTACAATAACGAGCGGATAAGCCTGAAGCTGAAAGGCCTGAGTCCGGTTGAATACCGAACCCAGGCCTTG
AAAGCCGCTTAACATGAACTGTCCAACTTTATGGGGTCAGTCCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 522 bp | 173 aa | 52 | 573 | + | No |
Description : First part of the transposase
ORF sequence :
MATPKYSLELRLSVVNHFLSGRDGTRRTAEIFGVERTSVRRWVRAFQLHGIDGITWKNDSHSADFRLIVVQAVLNEELSMREAAARFNISNETVVRRWVS
VYNDAGPEVLRNMKRGRPREMTRKTTPPPVDAVLEQLSPEELRAELRYLRAENAYLKKLKALVQSEKDGKKPK
VYNDAGPEVLRNMKRGRPREMTRKTTPPPVDAVLEQLSPEELRAELRYLRAENAYLKKLKALVQSEKDGKKPK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 927 bp | 308 aa | 486 | 1412 | + | No |
Description : Second part of the transposase
ORF sequence :
ASLSAGGECLSKKAESLGSKRKRRQKAQIISELRQGHALNDLLRAAGMSRSTWYHNISAMKRTDKHAGLKARIGDIYHHHKGRYGYRRITLSLRKEGIAV
NHKTVQRLMVELSLRSLIRVKKYRSWKGETGKAAPNILNRNFCASKSNEKWVTDVTEFSLQGKKLYLSPVLDLFNREIISYSLSERPVMDMVNTMLSDAL
SVLSPEESPLLHSDQGWQYRMAGYQEKLKAKGVVQSMSRKGNCLDNAVIENFFGTLKSECFYPGQFCSIEELKPAIQDYIHYYNNERISLKLKGLSPVEY
RTQALKAA
NHKTVQRLMVELSLRSLIRVKKYRSWKGETGKAAPNILNRNFCASKSNEKWVTDVTEFSLQGKKLYLSPVLDLFNREIISYSLSERPVMDMVNTMLSDAL
SVLSPEESPLLHSDQGWQYRMAGYQEKLKAKGVVQSMSRKGNCLDNAVIENFFGTLKSECFYPGQFCSIEELKPAIQDYIHYYNNERISLKLKGLSPVEY
RTQALKAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1361 bp | 453 aa | 52 | 1412 | + | Yes |
Chemistry : DDE
ORF sequence :
MATPKYSLELRLSVVNHFLSGRDGTRRTAEIFGVERTSVRRWVRAFQLHGIDGITWKNDSHSADFRLIVVQAVLNEELSMREAAARFNISNETVVRRWVS
VYNDAGPEVLRNMKRGRPREMTRKTTPPPVDAVLEQLSPEELRAELRYLRAENAYLKKAESLGSKRKRRQKAQIISELRQGHALNDLLRAAGMSRSTWYH
NISAMKRTDKHAGLKARIGDIYHHHKGRYGYRRITLSLRKEGIAVNHKTVQRLMVELSLRSLIRVKKYRSWKGETGKAAPNILNRNFCASKSNEKWVTDV
TEFSLQGKKLYLSPVLDLFNREIISYSLSERPVMDMVNTMLSDALSVLSPEESPLLHSDQGWQYRMAGYQEKLKAKGVVQSMSRKGNCLDNAVIENFFGT
LKSECFYPGQFCSIEELKPAIQDYIHYYNNERISLKLKGLSPVEYRTQALKAA
VYNDAGPEVLRNMKRGRPREMTRKTTPPPVDAVLEQLSPEELRAELRYLRAENAYLKKAESLGSKRKRRQKAQIISELRQGHALNDLLRAAGMSRSTWYH
NISAMKRTDKHAGLKARIGDIYHHHKGRYGYRRITLSLRKEGIAVNHKTVQRLMVELSLRSLIRVKKYRSWKGETGKAAPNILNRNFCASKSNEKWVTDV
TEFSLQGKKLYLSPVLDLFNREIISYSLSERPVMDMVNTMLSDALSVLSPEESPLLHSDQGWQYRMAGYQEKLKAKGVVQSMSRKGNCLDNAVIENFFGT
LKSECFYPGQFCSIEELKPAIQDYIHYYNNERISLKLKGLSPVEYRTQALKAA
Blast result :
Comments
ISEch2 is 86% aa similar to ISKpn1.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder submission (2018)
2] Glasner,J.D., Yang,C.H., Reverchon,S., Hugouvieux-Cotte-Pattat,N., Condemine,G., Bohin,J.P., Van Gijsegem,F., Yang,S., Franza,T., Expert,D., Plunkett,G. III, San Francisco,M.J., Charkowski,A.O., Py,B., Bell,K., Rauscher,L., Rodriguez-Palenzuela,P., Toussaint,A., Holeva,M.C., He,S.Y., Douet,V., Boccara,M., Blanco,C., Toth,I., Anderson,B.D., Biehl,B.S., Mau,B., Flynn,S.M., Barras,F., Lindeberg,M., Birch,P.R., Tsuyumu,S., Shi,X., Hibbing,M., Yap,M.N., Carpentier,M., Dassa,E., Umehara,M., Kim,J.F., Rusch,M., Soni,P., Mayhew,G.F., Fouts,D.E., Gill,S.R., Blattner,F.R., Keen,N.T. and Perna,N.T. (2011) J. Bacteriol. 193 (8), 2076-2077 .
2] Glasner,J.D., Yang,C.H., Reverchon,S., Hugouvieux-Cotte-Pattat,N., Condemine,G., Bohin,J.P., Van Gijsegem,F., Yang,S., Franza,T., Expert,D., Plunkett,G. III, San Francisco,M.J., Charkowski,A.O., Py,B., Bell,K., Rauscher,L., Rodriguez-Palenzuela,P., Toussaint,A., Holeva,M.C., He,S.Y., Douet,V., Boccara,M., Blanco,C., Toth,I., Anderson,B.D., Biehl,B.S., Mau,B., Flynn,S.M., Barras,F., Lindeberg,M., Birch,P.R., Tsuyumu,S., Shi,X., Hibbing,M., Yap,M.N., Carpentier,M., Dassa,E., Umehara,M., Kim,J.F., Rusch,M., Soni,P., Mayhew,G.F., Fouts,D.E., Gill,S.R., Blattner,F.R., Keen,N.T. and Perna,N.T. (2011) J. Bacteriol. 193 (8), 2076-2077 .