ISCji1
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| KJ575543 | ND | Comamonas jiangduensis | Comamonas jiangduensis C-X2B |
DNA section
IS Length : 871 bp
Ends
IR Length : 15
IRL : AGTGGCTAACACCACTTCCCGTGAGAGCCCGTCAGAGCGGTTATGCTCGC
IRR : AGTGGCTAACACCACCGATCCACGAAGCGCGCACAGATGATCGCTGCCGC
Comments : Atypical ends : the rules for this family subgroup are ends beginning with GG or GA. Here, the ends are determined by the duplication at the insertion site.
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTTAGATGCA | CTAAG | CACATAATTG | 5 |
DNA sequence
AGTGGCTAACACCACTTCCCGTGAGAGCCCGTCAGAGCGGTTATGCTCGCAGCCCTCATGGCACGACGACCCGTAAGCAAAGAACTGTGGCGACAACTAC
AGCCCCTGATCCCGGCCTTTGTGCCTTCTGCCAAAGGCGGTGCACGCAAGCTCGCAATCAGCGATGAAGCCGCCCTCAACGGTATCTTGTTCGTGCTGCA
AACAGGCATTCCCTGGGAAGACCTTCCCCAATCTCTTGGGTACGGCAGCGGTATGACTTGCTGGCGGCGCCTGCGCGACTGGAATGCCGCGGGTGTCTGG
GAGCAGTTGCACCAGGCCATGCTGACTCGCTTGCGTGAACATGACCAAATCGATTGGAGCCGGGCCAGCATTGATGGCTCCTCGGTACCAAGCCCCCGGG
GGGCCAGGAAACAGGGCTCAACCCCACGGACAGAGGCAAGCTCGGCTCCAAGCGACACATCGTTGTAGACGCCAGAGGCATCCCGCTGGTGATCTTGGTA
AGCGGGGCGAACAGGCATGACTCCAAGATGTTCGAGAAGTGTGTGGATGCGATTCCTGCGATTGCAGGCTTGGCAGGACGCCCCCGTAAAAGGCCAGTCA
AGCTGCACGCTGACAAAGGCTACGACTTCAAGCGATGCCGAGCCCACCTGAGGCAGCGGGGCATCATTGGCCGGATTGCCAGACGAGGCATTGAAAGCAG
CGAGCGGCTGGGCAAGCACCGGTGGGTGGTGGAGAGGACGCACAGCTGGTTTGCAGGCTTTGGCAAGCTGCGAATCCGCTTTGAAAGACGGCTGGATATC
CACGAGGCGCTGCTGAAGTTGGCGGCAGCGATCATCTGTGCGCGCTTCGTGGATCGGTGGTGTTAGCCACT
AGCCCCTGATCCCGGCCTTTGTGCCTTCTGCCAAAGGCGGTGCACGCAAGCTCGCAATCAGCGATGAAGCCGCCCTCAACGGTATCTTGTTCGTGCTGCA
AACAGGCATTCCCTGGGAAGACCTTCCCCAATCTCTTGGGTACGGCAGCGGTATGACTTGCTGGCGGCGCCTGCGCGACTGGAATGCCGCGGGTGTCTGG
GAGCAGTTGCACCAGGCCATGCTGACTCGCTTGCGTGAACATGACCAAATCGATTGGAGCCGGGCCAGCATTGATGGCTCCTCGGTACCAAGCCCCCGGG
GGGCCAGGAAACAGGGCTCAACCCCACGGACAGAGGCAAGCTCGGCTCCAAGCGACACATCGTTGTAGACGCCAGAGGCATCCCGCTGGTGATCTTGGTA
AGCGGGGCGAACAGGCATGACTCCAAGATGTTCGAGAAGTGTGTGGATGCGATTCCTGCGATTGCAGGCTTGGCAGGACGCCCCCGTAAAAGGCCAGTCA
AGCTGCACGCTGACAAAGGCTACGACTTCAAGCGATGCCGAGCCCACCTGAGGCAGCGGGGCATCATTGGCCGGATTGCCAGACGAGGCATTGAAAGCAG
CGAGCGGCTGGGCAAGCACCGGTGGGTGGTGGAGAGGACGCACAGCTGGTTTGCAGGCTTTGGCAAGCTGCGAATCCGCTTTGAAAGACGGCTGGATATC
CACGAGGCGCTGCTGAAGTTGGCGGCAGCGATCATCTGTGCGCGCTTCGTGGATCGGTGGTGTTAGCCACT
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 426 bp | 141 aa | 43 | 468 | + | No |
Description : First part of the transposase
ORF sequence :
MLAALMARRPVSKELWRQLQPLIPAFVPSAKGGARKLAISDEAALNGILFVLQTGIPWEDLPQSLGYGSGMTCWRRLRDWNAAGVWEQLHQAMLTRLREH
DQIDWSRASIDGSSVPSPRGARKQGSTPRTEASSAPSDTSL
DQIDWSRASIDGSSVPSPRGARKQGSTPRTEASSAPSDTSL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 492 bp | 163 aa | 375 | 866 | + | No |
Description : Second part of the transposase
ORF sequence :
WLLGTKPPGGQETGLNPTDRGKLGSKRHIVVDARGIPLVILVSGANRHDSKMFEKCVDAIPAIAGLAGRPRKRPVKLHADKGYDFKRCRAHLRQRGIIGR
IARRGIESSERLGKHRWVVERTHSWFAGFGKLRIRFERRLDIHEALLKLAAAIICARFVDRWC
IARRGIESSERLGKHRWVVERTHSWFAGFGKLRIRFERRLDIHEALLKLAAAIICARFVDRWC
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 824 bp | 274 aa | 43 | 866 | + | Yes |
Chemistry : DDE
ORF sequence :
MLAALMARRPVSKELWRQLQPLIPAFVPSAKGGARKLAISDEAALNGILFVLQTGIPWEDLPQSLGYGSGMTCWRRLRDWNAAGVWEQLHQAMLTRLREH
DQIDWSRASIDGSSVPSPRGGQETGLNPTDRGKLGSKRHIVVDARGIPLVILVSGANRHDSKMFEKCVDAIPAIAGLAGRPRKRPVKLHADKGYDFKRCR
AHLRQRGIIGRIARRGIESSERLGKHRWVVERTHSWFAGFGKLRIRFERRLDIHEALLKLAAAIICARFVDRWC
DQIDWSRASIDGSSVPSPRGGQETGLNPTDRGKLGSKRHIVVDARGIPLVILVSGANRHDSKMFEKCVDAIPAIAGLAGRPRKRPVKLHADKGYDFKRCR
AHLRQRGIIGRIARRGIESSERLGKHRWVVERTHSWFAGFGKLRIRFERRLDIHEALLKLAAAIICARFVDRWC
Blast result :
Comments
ISCji1 is 92% aa similar to ISCte7.
The third ORF is the putative ORFAB reconstructed in silico by possible -1 frameshift.
ISCji1 is inserted into a class1 integron.
The third ORF is the putative ORFAB reconstructed in silico by possible -1 frameshift.
ISCji1 is inserted into a class1 integron.
References
1] Sally Partridge (2018) Direct submission.
2] Liang,J. and Lin,M. (2014) Direct GenBank submission.
2] Liang,J. and Lin,M. (2014) Direct GenBank submission.